|
Antibodies to Synaptosomal Proteins
|
| From the Laboratories
|
Antigen
(Clone)
IgG |
Name
(Source)
used in |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
Synaptophysin
(poly)
|
antibodies from
Fukawa et al.,
Reference:
Ziskin, 2007 |
|
polyclonal rabbit
|
|
|
|
Synaptotagmin
(mono)
|
anti- synaptotagmin
Reinhard Jahn
Max-Planck-Institute
Reference:
Ferguson, 2007 |
|
monoclonal mouse
|
|
|
|
VAMP2
(69.1)
|
anti-VAMP2
Reinhard Jahn
Max-Planck-Institute
Reference:
Ferguson, 2007 |
|
monoclonal mouse
|
|
|
|
| From the Manufacturers
|
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
Complexin-1,2
(poly)
|
Synaptic Systems
Cat# 122002 |
immunogen = synthetic peptide KYLPGPLQDMFKK (aa 122 - 134 in human) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue |
polyclonal rabbit
(antiserum, lyophilized.) |
IC, IH(P), IP, WB |
recognizes complexin 1 and 2 |
human, rat, mouse, electric ray |
Munc-18
(poly)
IgG
|
Axxora
Cat #
ALX-210-247
Reference:
Ihara, 2007 |
immunogen = synthetic peptide corresponding to aa 58-70 (V58EDINKRREPIPS70) of human Munc-18 |
polyclonal rabbit
(liquid, IgG fraction, affinity purified, PBS, BSA, azide) |
IP, WB |
~67 kDa Munc-18 |
rat, mouse |
Munc-18
(31)
IgG1
|
BD Transduction
Cat# 610336/7
Reference:
Burré J, 2010 |
immunogen = Rat Munc-18-1, aa. 381-567, near the C-terminal |
monoclonal mouse
(aqueous buffered solution, BSA, glycerol, azide) |
IF, IH, IP, WB |
recognizes Munc-18, 68 kDa |
human, rat, mouse |
SNAP 25
(SMI-81)
IgG1
|
Covance Research Products, Inc.
Cat# SMI-81R
from Sternberger Monoclonals
Reference:
Burré J, 2010 |
immunogen =
whole, unaltered SNAP-25 (epitope lies in the amino end of the C-terminal of SNAP-25) |
monoclonal mouse
(ascites fluid, azide) |
ELISA, IC, IH, WB |
detects SNAP 25; visualizes synapse-rich areas of brain and retina in a wide variety of mammalian and non-mammalian species |
various animal |
SNAP 25
(SP12)
IgG1
|
Covance Research Products, Inc.
Cat# MMS-614R |
immunogen =
crude synaptic preparation from the post mortem human brain. |
monoclonal mouse
(ascites fluid) |
ELISA, IC, WB |
detects SNAP 25; reacts with native, denatured and recombinant pre-synaptic membrane SNAP-25 (A and B isoforms) expressed in COS cells |
human, rodent (various), pig, cat |
SNAP 25
(mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C11536 |
immunogen =
crude human synaptic immunoprecipitate |
monoclonal mouse
(supernatant) |
ELISA, IH(P), WB |
SNAP 25 |
human, rat, hamster, pig |
SNAP 25
(mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C10246 |
immunogen =
crude human synaptic immunoprecipitate |
monoclonal mouse
(ascites) |
ELISA, IH(P), WB |
synaptosomal associated protein 25 (SNAP 25); recognizes fusion protein from COS cells but not from bacterial |
human, rat, hamster, porcine |
SNAP 25
(mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C10247 |
immunogen =
crude human synaptic immunoprecipitate |
monoclonal mouse
(protein A purified) |
ELISA, IH(P), WB |
synaptosomal associated protein 25 (SNAP 25); recognizes fusion protein from COS cells but not from bacterial |
human, rat, hamster, porcine |
SNAP 25
(mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C26789 |
|
monoclonal mouse
(supernatant) |
ELISA, IH(P), WB |
recognizes SNAP 25 |
monkey, human, rat, mouse, bovine, chicken |
SNAP 25
(mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C10243 |
immunogen = synaptic vesicle-containing fractions IP from human brain homogenates using anti-human synaptophysin monoclonal antibodies |
monoclonal mouse
(protein G purified) |
IH(P), WB |
recognizes both the A and B isoforms of SNAP25, Mr ~27kDa |
human, rat, mouse, hamster, porcine, bovine, xenopus
(tick) |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
SNAP 25
(mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C26790 |
|
monoclonal mouse
|
IH, WB |
recognizes SNAP 25 |
human |
SNAP 25
(mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C10249 |
immunogen = synaptic vesicle-containing fractions immunoprecipitated |
monoclonal mouse
(protein G purified) |
IH, WB |
recognizes SNAP 25 both A and B isoforms |
human, rat, mouse, hamster, porcine, bovine, xenopus |
SNAP 25
(mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C10250 |
immunogen = crude human synaptic immunoprecipitate |
monoclonal mouse
(supernatant) |
IH(P) |
recognizes SNAP 25 |
human, rat, hamster, porcine |
SNAP 25
(poly)
|
LifeSpan Biosciences, Inc.
Cat# LS-C20213
available in USA only |
immunogen = synthetic peptide of
C-terminus of SNAP25 (D E A N Q R A T K M L G S G) |
polyclonal goat
(affinity purified) |
ELISA, WB |
recognizes synaptosomal associated protein 25 (SNAP 25); recognise both reported isoforms NP_003072 and NP_570824 |
human, rat, mouse, dog |
SNAP 25
(poly)
|
LifeSpan Biosciences, Inc.
Cat# LS-C30224 |
immunogen = synthetic peptide |
polyclonal rabbit
(affinity purified) |
ELISA, WB |
recognizes synaptosomal associated protein 25 (SNAP 25) |
human, rat, mouse, dog |
SNAP 25
(poly)
|
LifeSpan Biosciences, Inc.
Cat# LS-C10245 |
immunogen = synthetic peptide from the C-terminal heptadeapeptide of mouse SNAP-25 corresp. to residues Cys+ 190-206: C R I D E A N Q R A T K M L G S G |
polyclonal rabbit
(unpurified serum) |
IH, WB |
recognizes synaptosomal associated protein 25 (SNAP 25); will not detect SNAP 25 following proteolysis with botulinum neurotoxins A and E; no crossreactivity with SNAP 23 |
rat, mouse, bovine |
SNAP 25
(poly)
|
LifeSpan Biosciences, Inc.
Cat# LS-C10248 |
immunogen = synthetic peptide corresponding to aa 195-206 |
polyclonal rabbit
(IgG fractionation) |
IC, WB |
recognizes SNAP 25 |
rat, mouse, bovine, zebra fish |
SNAP 25
(poly)
|
LifeSpan Biosciences, Inc.
Cat# LS-C10251 |
immunogen = synthetic peptide corresp. to aa195-206 from human SNAP25 |
polyclonal rabbit
(affinity purified) |
ELISA, WB |
recognizes synaptosomal associated protein 25 (SNAP 25) |
human |
SNAP 25
(SP 12)
IgG1
|
Sigma Aldrich
Cat# S5187 |
immunogen = crude human synaptic precipitate |
monoclonal mouse
(buffered aqueous solution, purified IgG, PBS, azide) |
ELISA, IB, IH |
synaptosomal associated protein 25 (SNAP 25) |
human, rat, hamster, pig |
SNAP 25
(poly)
|
Sigma Aldrich
Cat# S9684
Reference:
Yuan, 2008 |
immunogen = synthetic peptide corresp. to the N-terminal of human SNAP25 (aa 9-29) with C-terminally added lysine conj. to KLH |
polyclonal rabbit
(buffered aqueous solution, IgG fraction, PBS, azide) |
IB, IH |
synaptosomal associated protein 25 (SNAP 25) |
human, rat, mouse |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
SNAP 25
(poly)
|
Sigma Aldrich
Cat# HPA001830, Prestige Antibodies™, Powered by Atlas Antibodies |
immunogen = SNAP 25 recomb. protein epitope signature tag (PrEST)
|
polyclonal rabbit
(solution, affinity isolated, PBS, glycerol, azide) |
IH, WB |
synaptosomal associated protein 25 (SNAP 25) |
human |
SNAP-25
(poly)
IgG
|
Alomone
Cat #
ANR-001
Reference:
Ihara, 2007 |
immunogen = GST fusion protein with the full-length rat SNAP-25 |
polyclonal rabbit
(liquid, IgG fraction, protein-A purified, PBS, BSA, azide, Lyophilized) |
IH, IP, WB |
~25 kDa SNAP-25 |
rat, mouse, torpedo |
SNAP-25
(poly)
|
Chemicon and Upstate are Millipore
Cat# AB1762
Reference:
Adlard, 2010 |
immunogen = synthetic peptide from the C-terminal heptadeapeptide of mouse SNAP-25 (corresponding to residues Cys + 190-206: [C]RIDEANQR ATKMLGSG). |
polyclonal rabbit
(liquid serum, azide) |
IH, WB |
reacts with SNAP-25; no cross reactivity with SNAP-23 is expected |
bovine |
SNAP-25
(71.1)
IgG1
|
Synaptic Systems
Cat# 111011, purified, 111001, ascites
Reference:
Burré J, 2010 |
immunogen = recombinant full length rat SNAP 25 (epitope in the conserved N-terminal portion of the protein, aa 20-40 ) |
monoclonal mouse
(purified IgG or ascites, lyophilized) |
IC, IH, IP, WB |
specific for SNAP 25; recognizes splice variants SNAP 25A and B, and the Botulinum neurotoxin A and E cleavage products |
human, rat, mouse, various vertebrate
(various invertebrates) |
SNAP-25
(20 /SNAP-25)
IgG1
|
BD Transduction
Cat# 610366 |
immunogen = mouse SNAP-25 (epitope within aa. 8-29) |
monoclonal mouse
(aqueous, buffered, BSA, glycerol, azide) |
IF, WB |
25 kDa SNAP-25 |
rat, mouse |
SNAP-25
(BR05)
IgG2b
|
Wako Chemicals
Cat# 014-15771 |
myeloma cell, P3X63Ag8.653, fused with spleen cells of BALB/c mice which were immunized with rat brain synaptosome |
monoclonal mouse
|
IF, WB |
SNAP-25 |
rat |
Synapsin
(poly)
|
Cell Signaling
Cat# 2312
Reference:
Zhang, 2009 |
immunogen = a synthetic peptide corresponding to residues surrounding Ser9 of human synapsin I |
polyclonal rabbit
(liquid, affinity purified, HEPES, saline, BSA, glycerol) |
IF(F), WB |
detects endogenous levels of total synapsin protein, is expected to recognize all synapsin isoforms |
rat, mouse
(expect human) |
Synapsin I
(3C5)
IgG2a
|
Chemicon and Upstate are Millipore
Cat# MAB10137 |
immunogen = recomb. human synapsin I |
monoclonal mouse
(liquid, supernatant, azide) |
WB |
74kDa Synapsin I |
human |
Synapsin I
(poly)
|
Chemicon and Upstate are Millipore
Cat# AB1543P
Reference:
Goddard, 2007 |
immunogen = synapsin I (mixture of Ia & Ib) purified from bovine brain |
polyclonal rabbit
(Ig, affinity purified, no preservative, lyophilized) |
IC, IH, IP, WB |
Synapsin I (Mr of 80,000 and 77,000) |
human, rat, mouse, bovine |
Synapsin I
(poly)
|
Chemicon and Upstate are Millipore
Cat# AB1543
Reference:
Tampellini, 2007
Stevens, 2007
Nakamoto, 2007
(both, unknown formulation) |
immunogen = synapsin I (mixture of Ia & Ib) purified from bovine brain |
polyclonal rabbit
(liquid, serum, no preservative) |
IC, IH, IP, WB |
Synapsin I (Mr of 80,000 and 77,000) |
human, rat, mouse, bovine |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
Synapsin I
(poly)
|
Molecular Probes Invitrogen
Cat# A6442
Reference:
Trinchese, 2008 |
immunogen = bovine brain synapsin I |
polyclonal rabbit
(affinity purified, PBS, lyophilized) |
ELISA, IP, WB |
active against Synapsin I; it has little or no activity against synapsin II |
human, rat, mouse, bovine |
Synapsin I
(8)
IgG1
|
BD Transduction
Cat# 611392/3
Reference:
Giorgi, 2007 |
immunogen = rat synapsin Ia (epitope between aa. 528-541, in the proline-rich region) |
monoclonal mouse
(aqueous, purified, buffered, BSA, glycerol, azide) |
IF, WB |
80 kDa synapsin I |
rat, mouse |
Synapsin I
(poly)
|
Zymed/ Invitrogen
Cat# 51-5200 |
immunogen = purified bovine brain Synapsin 1 |
polyclonal rabbit
(affinity purified antiserum, ammonium bicarbonate, lyophilized) |
IH, IP, WB |
recognizes the synapsin 1 doublet on Western blots corresponding to Synapsin 1α and 1β; both punctate and diffuse immuno reactivity is present |
human, rat, mouse, bovine |
Synapsin I, phos Ser549
(poly)
|
Chemicon and Upstate are Millipore
Cat# AB9846 |
immunogen = synthetic peptide corresp. to amino acids surrounding the phospho Ser549 of rat Synapsin I |
polyclonal rabbit
(affinity purified Ig, liquid, HEPES, saline BSA, glycerol) |
WB |
Synapsin I phospho Ser549; ~78 kDa protein doublet in WB |
rat
(immunogen sequence identical in human, mouse, non-human primate, bovine and canine) |
Synapsin I, phos Ser549
(poly)
|
Zymed/ Invitrogen
Cat# 486100 |
immunogen = synthetic peptide corresp. to amino acids surrounding the phospho Ser549 of rat Synapsin I |
polyclonal rabbit
(affinity purified Ig, liquid, HEPES, saline BSA, glycerol) |
WB |
Synapsin I phospho Ser549; ~78 kDa protein doublet in WB |
rat
(immunogen sequence identical in human, mouse, non-human primate, bovine and canine) |
Synapsin I, phos Ser603
(poly)
|
Chemicon and Upstate are Millipore
Cat# AB5883
and AB5883-25UL |
immunogen = Synthetic peptide corresp. to amino acids surrounding the phospho Ser603 of rat Synapsin I |
polyclonal rabbit
(affinity purified Ig, liquid, HEPES, saline BSA, glycerol) |
WB |
Synapsin I phospho Ser603; ~78 kDa |
rat
(immunogen sequence identical in human, mouse and Xenopus) |
Synapsin I, phos Ser603
(poly)
IgG
|
Chemicon and Upstate are Millipore
Cat# 07-567 |
immunogen = peptide surrounding pSer603 of rat Synapsin I |
polyclonal rabbit
(protein-A purified, liquid, Tris, saline, azide, glycerol) |
WB |
Synapsin I phospho Ser603 |
rat
(expect human, mouse and bovine from homology) |
Synapsin I, phos Ser603
(poly)
|
Zymed/ Invitrogen
Cat# 36-9600 |
immunogen = synthetic phosphopeptide corresp. to the regions surrounding the phosphor Ser603 residue of rat Synapsin I |
polyclonal rabbit
(affinity purified, liquid, HEPES, BSA, glycerol) |
WB |
recognizes Synapsin I when phosphor. at Ser603 |
rat |
Synapsin I, phos Ser62 +67
(poly)
|
Zymed/ Invitrogen
Cat# 486200 |
immunogen = phosphopeptide corresponding to amino acid residues surrounding the phosphoserines 62 and 67 of Synapsin I. |
polyclonal rabbit
(affinity purified, liquid, HEPES, saline, BSA, glycerol) |
WB |
specific for ~78 kDa synapsin I doublet phosphorylated at serines 62 and 67 |
rat
(immunogen sequence identical in mouse and bovine) |
Synapsin I, phos Ser62,67
(poly)
|
Chemicon and Upstate are Millipore
Cat# AB9848 |
immunogen = synthetic peptide corresp. to amino acids surrounding the phospho Ser62,67 site of rat Synapsin I |
polyclonal rabbit
(affinity purified Ig, liquid, HEPES, saline BSA, glycerol) |
WB |
Synapsin I phospho Ser62,67; ~78 kDa protein doublet in WB |
rat
(immunogen sequence identical in mouse and bovine) |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
Synapsin I, phos Ser9
(poly)
|
Zymed/ Invitrogen
Cat# 36-9600 |
immunogen = synthetic phosphopeptide corresp. to the regions surrounding the phosphor Ser9 residue of rat Synapsin I |
polyclonal rabbit
(affinity purified, liquid, HEPES, BSA, glycerol) |
WB |
recognizes Synapsin I when phosphor. at Ser9 |
rat |
Synapsin IIa
(1)
IgG1
|
BD Transduction
610666/7
Reference:
Ihara, 2007 |
immunogen = rat synapsin IIa, aa. 451-586 |
monoclonal mouse
(aqueous, buffered, BSA, azide, glycerol, purified) |
IF, IH, IP, WB |
74 kDa synapsin IIa |
human, rat |
Synapsin phos Ser9
(poly)
|
Cell Signaling
Cat# 2311 |
immunogen = a synthetic peptide corresponding to residues surrounding Ser9 of human synapsin I |
polyclonal rabbit
(liquid, affinity purified, HEPES, saline, BSA, glycerol) |
WB |
detects endogenous levels of synapsin only when phosphorylated at serine 9, is expected to recognize all synapsin isoforms |
rat, mouse
(expect human) |
Synapsin-I
(poly)
|
Sigma Aldrich
Cat# S193
Reference:
Tampellini, 2009 |
immunogen = synapsin I from bovine brain |
polyclonal rabbit
(affinity isolated antibody, lyophilized from ammonium bicarbonate) |
ELISA, IB, IH(F), IP |
reacts with the synapsin I doublet. It may be used to localize and detect synapsin I (synapsins Ia and Ib are collectively referred to as synapsin I) in nerve terminals |
human, rat, mouse, bovine |
Synaptobrevin
(4E240)
IgM
|
Abcam
Cat# ab18013 |
immunogen = synaptic vesicle containing fractions which were immunoprecipitated from human brain homogenates by anti-human synaptophysin antibodies |
monoclonal mouse
(liquid, PBS, PMSF, glycerol,) |
WB |
detects a ~18kD protein in WB, corresp. to predicted size of VAMP; detects VAMP1 (aa 1-118) and VAMP2 (aa 33-96)-GST fusion proteins expressed in E. coli |
monkey, human, rat, guinea pig, mouse, hamster, rabbit, pig, sheep, cow, xenopus
(other species not tested) |
Synaptobrevin
(4H302)
IgM
|
Abcam
Cat# ab18015 |
immunogen = synaptic vesicle-containing fractions which were immunoprecipitated from human brain homogenates using anti-human synaptophysin monoclonal antibodies |
monoclonal mouse
(liquid, ascites, no preservative) |
WB |
reacts with the VAMP1 (aa 1-118) and the VAMP2 (aa 33-96)-GST fusion proteins expressed in E. coli |
human, rat, mouse, hamster, pig, cow, xenopus |
Synaptobrevin
(SP10)
IgM
|
Abcam
Cat# ab24735 |
immunogen =
tissue / cell preparation -a crude synaptic preparation of post mortem human brain
|
monoclonal mouse
(liquid, ascites, no preservative) |
ELISA, IH, WB |
reacts with native, denatured and recombinant synaptic vesicle proteins Synaptobrevin 1 (aa 1-118) and Synaptobrevin 2 expressed in a bacterial system (aa 33-96)
|
human, rodent (various), pig
(other species not tested) |
Synaptobrevin
(SP11)
IgG1
|
Abcam
Cat# ab11104 |
immunogen = crude human synaptic immunoprecipitate |
monoclonal mouse
(liquid, PBS, azide, protein G purified) |
ELISA, IH(P), WB |
recognises the human synaptic vesicle protein synaptobrevin, VAMP; it is not known whether it recognises VAMP1 or VAMP2 |
human, pig
(not rat; other species not tested) |
Synaptobrevin
(poly)
IgG
|
Abcam
Cat# ab28362 |
immunogen = details unavailable |
polyclonal sheep
(liquid, PBS, azide) |
IP, WB |
VAMP (Synaptobrevin)-1/2 |
rat |
Synaptobrevin
(SP10)
IgM
|
Novus Biologicals
Cat# NB 600-780 |
immunogen = information is considered commercially sensitive; Epitope is within aa 1-118 on VAMP1 and aa 33-96 on VAMP2
|
monoclonal mouse
(liquid, PBS, azide) |
WB |
recognises synaptobrevin (VAMP) and is reactive with both VAMP1 and VAMP2 |
human, rat, hamster, pig |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
Synaptobrevin 1
(mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C10971 |
immunogen =
crude synaptic immunoprecipitate (human) |
monoclonal mouse
(ascites) |
ELISA, IH, WB |
VAMP-1 and VAMP-2 fusion protein |
human, rat, hamster, porcine, salamander |
Synaptobrevin 1
(mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C10972 |
immunogen =
IP synaptic vesicle-containing fractions from human brain homogenates |
monoclonal mouse
(protein G purified) |
IH, WB |
VAMP-1 and VAMP-2 fusion protein expressed in E. coli |
monkey, human, rat, guinea pig, mouse, hamster, rabbit, porcine, sheep, bovine, xenopus |
Synaptobrevin 1
(poly)
|
LifeSpan Biosciences, Inc.
Cat# LS-C26481
available in USA only |
|
polyclonal sheep
|
IP, WB |
Synaptobrevin 1 |
human |
Synaptobrevin 1
(poly)
|
LifeSpan Biosciences, Inc.
Cat# LS-C10980 |
immunogen = synthetic peptide corresp. to aa 1-18 (M S A P A Q P P A E G T E G A A P G C) of rat VAMP-1 (vesicle associated membrane protein-1) |
polyclonal rabbit
(affinity purified) |
IP, WB |
Synaptobrevin 1 (~15kDa) |
human, rat, mouse, dog |
Synaptobrevin/VAMP-1
(poly)
|
Synaptic Systems
Cat# 104 002 |
immunogen = synthetic peptide SAPAQPPAEGTEG (aa 2-14 in rat synaptobrevin 1) coupled to |
polyclonal rabbit
(lyophilized; antiserum) |
IC, IH, IP, WB |
specific for VAMP 1; no cross reactivity to VAMP 2 and VAMP 3 |
human, rat, mouse, hamster
(no signal in chicken) |
Synaptobrevin/VAMP-1,2,3
(10.1)
IgG1
|
Synaptic Systems
Cat# 104 001 |
immunogen = electrophoretically purified rat VAMP 1 |
monoclonal mouse
(lyophilized; ascites) |
IC, IH, IP, WB; not IC on non-neuronal cells |
Specific for all three isoforms VAMP 1, 2, 3.
reacts with the conserved middle-portion of the molecule. |
human, rat, mouse, various animal |
Synaptobrevin/VAMP-1,2,3
(poly)
|
Synaptic Systems
Cat# 104 102 |
immunogen = recombinant protein of the cytoplasmic part of rat cellubrevin (aa 1 - 81) |
polyclonal rabbit
(lyophilized; antiserum) |
IC, IP, WB |
Specific for all three isoforms VAMP 1, 2, 3.
recommended for IC on non-neuronal cells |
human, rat, mouse
(others not tested) |
Synaptobrevin/VAMP-1,2,3
(poly)
|
Synaptic Systems
Cat# 104 203 |
immunogen = recombinant protein of the cytoplasmic part of rat cellubrevin (aa 1 - 81) |
polyclonal rabbit
(lyophilized; affinity purified, RSA for stabilization) |
IC, WB |
Specific for all three isoforms VAMP 1, 2, 3 |
human, rat, mouse
(others not tested) |
Synaptobrevin/VAMP-2
(poly)
IgG1
|
Synaptic Systems
Cat# 104 202 |
immunogen = synthetic peptide (SATAATVP PAAPAGEG) corresponding to residues 2-17 of Synaptobrevin 2 in rat, coupled to KLH |
polyclonal rabbit
(lyophilized; antiserum) |
IC, IH, IP, WB |
Synaptobrevin2/ VAMP2; no cross with VAMP1 or VAMP3; recognizes the Botulinumtoxin B cleavage product (aa 1-76) with reduced affinity |
human, rat, mouse, hamster
(no signal in chicken) |
Synaptobrevin-2
(poly)
IgG
|
Wako Chemicals
Cat# 018-15791 |
synthetic polypeptide (residues 1-20) of rat synaptobrevin 2, conj. with KLH |
polyclonal rabbit
|
IF, WB |
Synaptobrevin-2 |
rat |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
Synaptobrevin-2/VAMP-2
(69.1)
IgG1
|
Synaptic Systems
Cat# 104211
available fluorescence labelled as 104211C3
References:
Burré J, 2010
Ihara, 2007
|
immunogen = synthetic peptide (SATAATVP PAAPAGEG) corresponding to residues 2-17 of rat Synaptobrevin 2, coupled to KLH |
monoclonal mouse
(lyophilized, purified IgG) |
IH(P), IP, WB; IC for 1042111C3; ELISA capture |
specific for Synaptobrevin2/ VAMP2; no cross with VAMP1 or VAMP3 |
human, rat, mouse, hamster
(no signal in chicken) |
Synaptogyrin 2
(poly)
IgG
|
Abcam
Cat# ab31285 |
immunogen =
synthetic peptide: C I Y G E G Y S N A H E S K Q, corresp. to internal seq. aa 42-57 of human Synaptogyrin 2
|
polyclonal goat
(liquid, tris-buffered saline, BSA, azide, affinity purified) |
ELISA, WB |
Synaptogyrin 2 |
human
(expect mouse, chimpanzee due to seq. homology; other species not tested) |
Synaptogyrin 2
(poly)
IgG
|
Novus Biologicals
Cat# NB 100-1490 |
immunogen =
peptide with seq. C I Y G E G Y S N A H E S K Q, from the internal of the protein seq. according to NP_004701 |
polyclonal goat
(liquid, tris-saline, BSA, azide) |
WB |
Synaptogyrin 2 |
human
(expect mouse and chimpanzee due to seq. homology) |
Synaptogyrin 3
(poly)
IgG
|
Abcam
Cat# ab26059 |
immunogen =
synthetic peptide: C Q R T A P G P A T T Q A G D, corresp. to C- terminal aa 131-144 of human Synaptogyrin 3
|
polyclonal goat
(liquid, tris-buffered saline, BSA, azide, affinity purified) |
ELISA |
Synaptogyrin 3 |
human, chimpanzee, rat, dog |
Synaptogyrin 3
(poly)
IgG
|
Novus Biologicals
Cat# NB 100-1484 |
immunogen =
synthetic peptide C Q R T A P G P A T T Q A G D representing the C-terminus of the human protein (resides 131-144), according to NP_004200 |
polyclonal goat
(liquid, tris-saline, BSA, azide) |
ELISA, WB |
Synaptogyrin 3 |
human, chimpanzee, rat, dog
(other species not tested) |
Synaptogyrin 4
(poly)
IgG
|
Abcam
Cat# ab27480 |
immunogen =
synthetic peptide: H I P K S L Q E L A N S C, corresp. to aa 2-14 of human Synaptogyrin 4
|
polyclonal goat
(liquid, tris saline, BSA, azide, affinity purified) |
ELISA, WB |
Synaptogyrin 4 |
human
(other species not tested) |
Synaptogyrin 4
(poly)
IgG
|
Novus Biologicals
Cat# NB 300-494 |
immunogen =
synthetic peptide H I P K S L Q E L A N S-C, representing the N-terminus of the human protein sequence according to NP_036583. |
polyclonal goat
(liquid, tris-saline, BSA, azide) |
ELISA, WB |
Synaptogyrin 4 |
human |
Synaptophysin
(4E206)
IgM
|
Abcam
Cat# ab18008 |
immunogen =
synaptic vesicle-containing fractions immunoprecipitated from human brain homogenates using anti-human Synaptophysin monoclonal antibodies
|
monoclonal mouse
(liquid, PBS, PMSF, glycerol, no preservative, purified) |
IH(P), IP, WB |
Synaptophysin |
human, rat, mouse, cow |
Synaptophysin
(4H255)
IgM
|
Abcam
Cat# ab18007 |
immunogen =
synaptic vesicle-containing fractions immunoprecipitated from human brain homogenates using anti-human Synaptophysin monoclonal antibodies
|
monoclonal mouse
(liquid, PBS, ascites, glycerol, PMSF) |
WB |
Synaptophysin |
human, rat, mouse, cow |
Synaptophysin
(SP11)
|
Abcam
Cat# ab16659 |
immunogen =
synthetic peptide human
|
monoclonal rabbit
(liquid, supernatant, azide) |
IH(F, P), WB |
Synaptophysin |
human
(other species not tested) |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
Synaptophysin
(SP11)
IgG
|
Abcam
Cat# ab27601 |
immunogen = synthetic peptide of human Synaptophysin
|
monoclonal rabbit
(liquid, PBS, BSA, azide, protein A purified; prediluted) |
IH(P) |
recognises an epitope on the C terminus human Synaptophysin |
human
(other species not tested) |
Synaptophysin
(SP15)
IgM
|
Abcam
Cat# ab11105 |
immunogen = crude human synaptic immunoprecipitate |
monoclonal mouse
(liquid, supernate, purified, gel filtration, PBS, azide) |
ELISA, IH(P), WB |
reacts with neuroendocrine cells and with a wide spectrum of neuroendocrine neoplasms of neural type; reacts with synaptophysin expressed in CHO cells |
human, rat, hamster, pig
(others not tested) |
Synaptophysin
(SP17)
IgM
|
Abcam
Cat# ab24729 |
immunogen =
tissue / cell preparation of a crude synaptic preparation from the post mortem brain (Human
|
monoclonal mouse
(liquid, ascites, no preservative) |
ELISA, IC, WB |
reacts with native, denatured and recombinant synaptic vesicle protein synaptophysin expressed in CHO cells |
human, rat, pig
(other species not tested) |
Synaptophysin
(SY38)
IgG1
|
Abcam
Cat# ab8049 |
|
monoclonal mouse
(diluted ascites, azide) |
IH(F, P) |
reacts with neuroendocrine cells and with a wide spectrum of neuroendocrine neoplasms of neural type |
rat, mouse, cow
(others not tested) |
Synaptophysin
(SYP02)
IgG1
|
Abcam
Cat# ab6245 |
immunogen = synthetic peptide encoding a region near the C-terminal end of synaptophysin |
monoclonal mouse
(liquid, protein-A, PBS, azide) |
IH(F, P) |
38 kDa synaptophysin; reacts with neuroendocrine neoplasms of neural as well as epithelial types |
human
(others not tested) |
Synaptophysin
(YE269)
IgG
|
Abcam
Cat# ab32127 |
immunogen =
synthetic peptide corresponding to residues in C- terminus (cytoplasmic domain) of human Synaptophysin
|
monoclonal rabbit
(liquid, supernatant, tris-glycine saline, BSA, glycerol, azide) |
IC, IF, IH(P), WB;
not FACS or IP |
Synaptophysin |
human, rat, mouse
(expect bovine due to seq. homology; other species not tested) |
Synaptophysin
(poly)
IgG
|
Abcam
Cat# ab7837 |
immunogen = synthetic human synaptophysin peptide |
polyclonal rabbit
(antiserum, Tris, carrier, azide, prediluted) |
IH(F, P) |
reacts with hydrophilic peptide sequence of synaptophysin, in a wide spectrum of neuroendocrine neoplasms of neural type |
human
(others not tested) |
Synaptophysin
(poly)
|
Abcam
Cat# ab8547 |
immunogen = synthetic human peptide |
polyclonal rabbit
(antiserum, buffered, green food dye, azide, predilute) |
IH(P) |
reacts with neuroendocrine cells and with a wide spectrum of neuroendocrine neoplasms of neural type |
human
(others not tested) |
Synaptophysin
(poly)
IgG
|
Abcam
Cat# ab23754 |
immunogen =
synthetic peptide derived from within residues 1-100 of human Synaptophysin, conjugated to KLH
|
polyclonal rabbit
(liquid, PBS, BSA, azide, affinity purified) |
WB |
Synaptophysin |
human
(expect mouse (86%), rat (86%) and cow (100%) due to seq. homology; other species not tested) |
Synaptophysin
(poly)
IgG
|
Abcam
Cat# ab32594 |
immunogen =
synthetic peptide, G P G G Y G P Q D S Y G P Q G G Y Q P D, corresp. to aa 253-272 of rat Synaptophysin
|
polyclonal rabbit
(liquid, PBS, BSA, azide, affinity purified) |
WB |
Synaptophysin |
rat, mouse, sheep, cow
(expect human due to seq. homology; other species not tested) |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
Synaptophysin
(poly)
IgG
|
Abcam
Cat# ab14692 |
immunogen =
synthetic peptide, F A T C G S Y S G E L Q L S V D C A N K T E, corresp. to aa 41-62 of human Synaptophysin
|
polyclonal rabbit
(liquid, azide, affinity purified) |
IH(F), IP, WB |
synaptophysin; no cross-reaction with other synaptic vesicle proteins such as synaptotagmin, GAP45 and SNAP25 proteins |
human, rat, mouse
(expect cow due to seq. homology; other species not tested) |
Synaptophysin
(EP10)
IgG1
|
A B R - Thermo Scientific
Cat# SA 3066 |
immunogen = human crude synaptosomal preparation |
monoclonal mouse
(purified Ig, concentrate, azide) |
IH, WB |
Synaptophysin (38 kD) in fibres and in nerve endings |
human |
Synaptophysin
(SP15)
IgM
|
A B R - Thermo Scientific
Cat# SA 3068 |
immunogen = human crude synaptosomal preparation |
monoclonal mouse
(purified Ig, concentrate, azide) |
IH, WB |
Synaptophysin (38 kD) in fibres and in nerve endings |
human, rat, hamster, pig |
Synaptophysin
(SY38)
IgG1
|
A B R - Thermo Scientific
Cat# SA 3064 |
immunogen = synaptophysin from presynaptic vesicles |
monoclonal mouse
(serum, concentrate, azide) |
IH, WB |
Synaptophysin (38 kD) in fibres and in nerve endings |
human, rat, mouse, bovine |
Synaptophysin
(mono)
IgG1
|
Accurate
Cat# ICCMSY |
immunogen = synaptophysin from presynaptic vesicles |
monoclonal mouse
(ascites,PBS, BSA, azide) |
IH(F, P) |
Synaptophysin |
|
Synaptophysin
(mono)
|
Accurate
cat #
NBM AB382C |
|
monoclonal mouse
(concent,high titer) |
IH(F, P); (w/NB reagent) |
Synaptophysin |
human |
Synaptophysin
(mono)
|
Accurate
cat #
NBM AB382P |
|
monoclonal mouse
(ready to use) |
IH(F, P); (w/NB reagent) |
Synaptophysin |
human |
Synaptophysin
(27G12)
|
Accurate
cat #
MED CLA546 |
|
monoclonal mouse
(supernat) |
IH(F, P), WB |
Synaptophysin, 38 kD |
human |
Synaptophysin
(27G12)
|
Accurate
cat#
MED CLA646 |
|
monoclonal mouse
(ready to use) |
|
Synaptophysin, 38 kD |
human |
Synaptophysin
(27G12)
|
Accurate
cat #
MED CLA647 |
|
monoclonal mouse
(ready to use) |
|
Synaptophysin, 38 kD |
human |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
Synaptophysin
(SP15)
|
Accurate
cat #
YSRT MCA1307 |
|
monoclonal mouse
(purified) |
ELISA, IH(P), WB |
Synaptophysin |
human, hamster, swine, various species |
Synaptophysin
(SVP-38)
|
Accurate
cat #
BMVD1130 |
|
monoclonal mouse
|
IH(F, P) |
Synaptophysin, 38 kD |
human, rat, guinea pig, swine |
Synaptophysin
(SY38)
|
Accurate
cat #
CBL203 |
|
monoclonal mouse
|
IH(F, P) |
Synaptophysin |
human |
Synaptophysin
(SY38)
|
Accurate
cat #
YM9013 |
|
monoclonal mouse
(dilute, ascites) |
IH(F, P) |
Synaptophysin, 38 kD |
rat, mouse, bovine |
Synaptophysin
(SY38)
|
Accurate
cat #
AXL899M |
|
monoclonal mouse
|
|
Synaptophysin |
|
Synaptophysin
(poly)
|
Accurate
cat #
MED CLA554 |
|
polyclonal rabbit
|
|
Synaptophysin |
|
Synaptophysin
(poly)
|
Accurate
cat #
MED CLA461 |
|
polyclonal rabbit
|
IH(F, P); not WB |
Synaptophysin |
human |
Home
