|
Antibodies to Presenilin-Complex Proteins, Nicastrin and Ubiquilin
|
| From the Laboratories
|
Antigen (Clone) IgG |
Name (Source) used in |
Immunogen (Epitope) |
Host (Formulation) |
Methods glossary |
Specificity |
Reactivity top |
APH-1a, pan (poly) IgG
|
2021 (Harald Steiner, Christian Haass Adolf-Butenandt-Institute; Ludwig-
Maximilians-University Munich) Paper Reference: Sato, 2007 |
immunogen = the loop three
domain of APH-1a (residues 103-115) |
polyclonal rabbit (liquid, affinity purified) |
IB |
pan-APH-1a antibody; allows detection of all APH-1a splice forms |
human |
Nicastrin (poly)
|
R302 D.Miller, P. Savam, Institute for Basic Research in Developmental Disorders Reference: Lazarov, 2006 |
|
polyclonal |
IF, IP, WB |
C-terminal 20 amino acids, 690-709, of Nicastrin |
|
Nicastrin (poly)
|
SP716 G Thinkaran, H Xu Paper Reference: Liu, Y, 2009 |
immunogen = synthetic peptide corresponding to residues 62-93 |
polyclonal rabbit (antiserum) |
IP, WB |
specific for Nicastrin |
mouse |
PEN-2 (poly)
|
anti-1638 PEN-2 (Ross Francis,
Exelixis, Inc.,
South San Francisco, CA) used
in paper by Steiner, 2002 |
N-terminus aa 4-15 of human PEN-2 |
polyclonal |
|
|
rat (reference: Francis, R., McGrath, G., ... Nye, J. S., and Curtis, D. (2002) Dev. Cell 3, 85-97) |
PEN-2 (poly)
|
CR8 (Tae-Wan Kim,
Center for Neurobiology and Behavior, Columbia University) used
in paper by Luo, 2003 |
C-terminal 25 aa of PEN-2 |
polyclonal |
WB |
|
|
PEN-2 (poly)
|
anti-PNT2 (Gopal Thinakaran,
Department of Neurobiology, University of Chicago) used
in paper by Luo, 2003 Reference: Liu, Y, 2009
|
N-terminal 26 aa of PEN-2 |
polyclonal rabbit |
WB |
|
|
PEN-2 (poly)
|
anti-PEN-2 (Masafumi Shimojo, et al,
Laboratory for Alzheimer's Disease, RIKEN Brain Science Institute, Wako-shi, Saitama, Japan Reference: Osawa, 2008 |
immunogen = synthesized peptide corresp. to human N-terminal sequence (NLERVSN EEKLNLC) |
polyclonal rabbit |
IB |
PEN-2, N-terminal |
human |
PEN-2 (poly)
|
anti-PEN-2 (Masafumi Shimojo, et al,
Laboratory for Alzheimer's Disease, RIKEN Brain Science Institute, Wako-shi, Saitama, Japan Reference: Osawa, 2008 |
immunogen = synthesized peptide corresp. to human C-terminal sequence (CALGDYLS FTIPLGTP) |
polyclonal rabbit |
IB |
PEN-2, C-terminal |
human |
| From the Manufacturers
|
Antigen (Clone) IgG |
Distributor (Producer) |
Immunogen (Epitope) |
Host (Formulation) |
Methods glossary |
Specificity |
Reactivity top |
APH 1b (poly) IgG
|
Abcam
cat# ab24614 |
immunogen = synthetic peptide from loop region of human Aph-1b; Epitope seq. C K L L K K A S E G L K S I N P G E T A P S M R |
polyclonal rabbit (liquid, PBS, glycerol, BSA, azide, affinity purified) |
IF, WB |
Aph 1b |
human (others not tested) |
APH 1b (poly) IgG
|
Abcam
cat# ab24613 |
immunogen = synthetic peptide containing the C- terminal seq. of human Aph 1b; Epitope seq. C Q D K N F L L Y N Q R S R Q D K N F L L Y N Q R S R |
polyclonal rabbit (liquid, PBS, BSA, glycerol, azide, affinity purified) |
IF, WB |
Aph-1b |
human (others not tested) |
APH1 (poly)
|
ABGENT
cat# AP6302a |
immunogen = synthetic peptide, 10-30 aa, at the N-term of first 110 aa of human APH1, conj. to KLH |
polyclonal rabbit (liquid, PBS, azide, protein G purified) |
ELISA |
Aph1 |
human (others not tested) |
APH1 (poly)
|
ABGENT
cat# AP6302b |
immunogen synthetic peptide, 10-30 aa, at the C-term of last 50 aa of human APH1 |
polyclonal rabbit (liquid, PBS, azide, protein G purified) |
ELISA |
Aph1 |
human (others not tested) |
APH1 (poly)
|
AMS Biotechnology
cat# AP6302a supplied by Abgent |
immunogen = synthetic peptide, 10-30 aa, at the N-term of first 110 aa of human APH1, conj. to KLH |
polyclonal rabbit (liquid, PBS, azide, protein G purified) |
ELISA |
Aph1 |
human (others not tested) |
APH1 (poly)
|
AMS Biotechnology
cat# AP6302b supplied by Abgent |
immunogen synthetic peptide, 10-30 aa, at the C-term of last 50 aa of human APH1 |
polyclonal rabbit (liquid, PBS, azide, protein G purified) |
ELISA |
Aph1 |
human (others not tested) |
APH-1 (poly)
|
A B R - Thermo Scientific
Cat# PA1-2010 Reference: Sato, 2007 |
immunogen = Synthetic peptide, KLH conjugated, corresponding to residues C(245)RRQEDSRVM VYSALRIPPED(265) of human APH-1aL/C-terminal |
polyclonal rabbit (liquid, affinity purified, PBS, BSA, azide, glycerol) |
IF, IP, WB |
recognizes APH-1 |
human, mouse |
APH-1 (poly) IgG
|
Acris Antibodies
Cat# SP5419P |
immunogen = peptide, C R R Q E D S R V M V Y S A L R I P P E D, corresp. to aa 245-265 at C-terminal domain of the long isoform of human Aph-1a, conj. to KLH |
polyclonal rabbit (liquid, PBS, BSA, glycerol, azide, affinity purified) |
IF, IP, WB |
Aph-1 |
human, mouse |
APH-1 (poly) IgG
|
Autogen Bioclear
cat# sc-30242 supplied by Santa Cruz |
immunogen = peptide mapping within an internal region of APH-1 of human origin. |
polyclonal goat (liquid, PBS, azide, gelatin, affinity purified) |
IF, WB |
18 kDa Aph-1 |
human, rat, mouse |
APH-1 (poly) IgG
|
Autogen Bioclear
cat# sc-30237 supplied by Santa Cruz |
immunogen = peptide mapping within an internal region of APH-1 of C.Elegans origin. |
polyclonal goat (liquid, PBS, azide, gelatin, affinity purified) |
IF, WB |
Aph-1 |
C. elegans |
Antigen (Clone) IgG |
Distributor (Producer) |
Immunogen (Epitope) |
Host (Formulation) |
Methods glossary |
Specificity |
Reactivity top |
APH-1 (poly) IgG
|
Autogen Bioclear
cat# sc-30239 supplied by Santa Cruz |
immunogen = peptide mapping at the C-terminus of APH-1 of C.Elegans origin. |
polyclonal goat (liquid, PBS, azide, gelatin, affinity purified) |
IF, WB |
Aph-1 |
C. elegans |
APH-1 (poly)
|
Chemicon and Upstate are Millipore
Cat# AB9214 |
immunogen = synthetic peptide from hAPH-1a
|
polyclonal rabbit (liquid, serum, PBS, no preservative) |
WB |
Aph-1 (~29kDa) |
mouse (others not tested) |
APH-1 (poly) IgG
|
Insight Biotechnology
Cat# SP5419P supplied by Acris available in UK and Ireland only |
immunogen = peptide, (C R R Q E D S R V M V Y S A L R I P P E D), corresp. to aa 245-265 from the human Aph-1aL/CT, conj. to KLH |
polyclonal rabbit (liquid, PBS, BSA, glycerol, azide, affinity purified) |
IF, IP, WB |
Aph-1 |
human, mouse |
APH-1 (poly) IgG
|
Insight Biotechnology
cat# sc-30242 supplied by Santa Cruz available in UK only |
immunogen = peptide mapping within an internal region of APH-1 of human origin. |
polyclonal goat (liquid, PBS, azide, gelatin, affinity purified) |
IF, WB |
18 kDa Aph-1 |
human, rat, mouse |
APH-1 (poly) IgG
|
Insight Biotechnology
cat# sc-30237 supplied by Santa Cruz available in UK only |
immunogen = peptide mapping within an internal region of APH-1 of C.Elegans origin. |
polyclonal goat (liquid, PBS, azide, gelatin, affinity purified) |
IF, WB |
Aph-1 |
C. elegans |
APH-1 (poly) IgG
|
Insight Biotechnology
cat# sc-30239 supplied by Santa Cruz available in UK only |
immunogen = peptide mapping at the C-terminus of APH-1 of C.Elegans origin. |
polyclonal goat (liquid, PBS, azide, gelatin, affinity purified) |
IF, WB |
Aph-1 |
C. elegans |
APH-1 (poly) IgG
|
Li Starfish
Cat# A-SP5419P supplied by Acris |
immunogen = peptide, C R R Q E D S R V M V Y S A L R I P P E D, corresp. to aa 245-265 at C-terminal domain of the long isoform of human Aph-1a, conj. to KLH |
polyclonal rabbit (liquid, PBS, BSA, glycerol, azide, affinity purified) |
IF, IP, WB |
Aph-1 |
human, mouse |
APH-1 (poly) IgG
|
Santa Cruz
cat# sc-30242 |
immunogen = peptide mapping within an internal region of APH-1 of human origin. |
polyclonal goat (liquid, PBS, azide, gelatin, affinity purified) |
IF, WB |
18 kDa Aph-1 |
human, rat, mouse |
APH-1 (poly) IgG
|
Santa Cruz
cat# sc-30237 |
immunogen = peptide mapping within an internal region of APH-1 of C.Elegans origin. |
polyclonal goat (liquid, PBS, azide, gelatin, affinity purified) |
IF, WB |
Aph-1 |
C. elegans |
APH-1 (poly) IgG
|
Santa Cruz
cat# sc-30239 |
immunogen = peptide mapping at the C-terminus of APH-1 of C.Elegans origin. |
polyclonal goat (liquid, PBS, azide, gelatin, affinity purified) |
IF, WB |
Aph-1 |
C. elegans |
Antigen (Clone) IgG |
Distributor (Producer) |
Immunogen (Epitope) |
Host (Formulation) |
Methods glossary |
Specificity |
Reactivity top |
APH-1 (poly) IgG
|
Vinci Biochem
Cat# PA1-2010 supplied by ABR |
immunogen = synthetic peptide corresponds to aa 245-265 from human APH-1a Long/CT |
polyclonal rabbit (IgG, affinity purified, PBS, BSA, azide, glycerol) |
IF, IP, WB |
C-Terminal domain of the long isoform of APH-1a |
human, mouse |
APH1, C-term (poly)
|
Interchim
cat# GL1750 supplied as AP6302B |
immunogen synthetic peptide, 10-30 aa, at the C-term of last 50 aa of human APH1 |
polyclonal rabbit (liquid, PBS, azide, protein G purified) |
ELISA |
Aph-1 |
human |
APH1, N-term (poly)
|
Interchim
cat# GL1750 supplied as AP6302A |
immunogen = synthetic peptide, 10-30 aa, at the N-term of first 110 aa of human APH1, conj. to KLH |
polyclonal rabbit (liquid, PBS, azide, protein G purified) |
ELISA |
Aph-1 |
|
APH1a (poly) IgG
|
Abcam
cat# ab12104 |
immunogen = synthetic peptide derived from residues 50-150 of human APH1a |
polyclonal rabbit (liquid, PBS, BSA, azide, affinity purified) |
WB |
recognises both isoforms of APH1a (encoded on chromosome 1)
|
human, rat, mouse (others not tested) |
APH1a (poly) IgG
|
Abcam
cat# ab19390 |
immunogen = synthetic peptide (C R R Q E D S R V M V Y S A L R I P P E D corresp. to C-terminal domain of the long isoform of aa 245-265 of human APH1a, conj. to KLH |
polyclonal rabbit (liquid, PBS, glycerol, PBS, azide, affinity purified) |
IF, IP, WB |
Anterior Pharynx Defective 1 (APH1a) |
human, mouse (others not tested) |
APH1a (poly) IgG
|
GeneTex
Cat# GTX12104 supplied by Abcam |
immunogen = synthetic peptide (A D E G L A S L S E D G R S P I S I R), conjugated to KLH, corresp. to C-terminal aa 97-115 of human APH1a |
polyclonal rabbit (liquid, PBS, BSA, azide, affinity purified) |
WB |
recognises both isoforms of APH1a (encoded on chromosome 1) |
human, mouse (others not tested) |
APH1a (poly) IgG
|
GeneTex
Cat# GTX19390 supplied by Abcam |
immunogen = synthetic peptide (C R R Q E D S R V M V Y S A L R I P P E D corresp. to C-terminal domain of the long isoform of aa 245-265 of human APH1a, conj. to KLH |
polyclonal rabbit (liquid, PBS, glycerol, PBS, azide, affinity purified) |
IF, IP, WB |
Anterior Pharynx Defective 1 (APH1a) |
human, mouse (others not tested) |
APH1a (poly) IgG
|
Novus Biologicals
cat# NB100-61660 |
immunogen = synthetic peptide, C-HVTDRSDARLQYG, representing the internal region of the sequence |
polyclonal goat (liquid, antisera, Tris-saline, BSA, azide, affinity purified) |
ELISA, WB |
~26kDa APH1a |
human (expect mouse, rat from homology) |
Aph-1a (poly) IgG
|
Euromedex
Cat# A2298-77 supplied by US Biological |
immunogen = synthetic peptide derived from the C-terminal region of human Aph-1a |
polyclonal rabbit (liquid, PBS, azide, glycerol, affinity purified) |
IP, WB |
Aph-1a long isoform only(~29kDa, fragment at ~23kDa) |
human, mouse |
Aph-1a (poly) IgG
|
Europa
Cat# UBA2298-77 supplied by US Biological |
immunogen = synthetic peptide derived from the C-terminal region of human Aph-1a |
polyclonal rabbit (liquid, PBS, azide, glycerol, affinity purified) |
IP, WB |
Aph-1a Long form only (~29kD, fragment at ~23kD) |
human, mouse |
Antigen (Clone) IgG |
Distributor (Producer) |
Immunogen (Epitope) |
Host (Formulation) |
Methods glossary |
Specificity |
Reactivity top |
Aph-1a (poly)
|
Histo-Line
Cat# 38-3600 supplied by Zymed |
immunogen = synthetic peptide derived from the C-terminal region of human Aph-1a |
polyclonal rabbit (liquid, PBS, azide, affinity purified) |
IP, WB |
Aph-1a (~23kDa) long isoform only |
human, mouse |
Aph-1a (poly) IgG
|
US Biological
Cat# A2298-77 |
immunogen = synthetic peptide derived from the C-terminal region of human Aph-1a |
polyclonal rabbit (liquid, PBS, azide, glycerol, affinity purified) |
IP, WB |
Aph-1a long isoform only(~29kDa, fragment at ~23kDa) |
human, mouse |
Aph-1a (poly)
|
Zymed/ Invitrogen
Cat# 38-3600 References: Tian Y, 2010 Deng, 2006 |
immunogen = synthetic peptide derived from the C-terminal region of human Aph-1a |
polyclonal rabbit (liquid, PBS, azide, affinity purified) |
IP, WB |
Aph-1a (~23kDa) long isoform only |
human, mouse |
APH-1aL (poly) IgG
|
Calbiochem EMD Biosciences
Cat# PC728 |
immunogen = synthetic peptide (C R R Q E D S R V M V Y S A L R I P P E D) corresp. to aa 245-265 of human and mouse Aph-1aL |
polyclonal rabbit (liquid, NEAT serum) |
IP, WB |
Aph-1aL protein (~30kDA) |
human, rat, mouse |
Aph-1aL C-Terminal (poly)
|
Covance Research Products, Inc.
Cat# PRB-550P Reference: Zhao, 2010 |
immunogen = human Aph-1aL/ C-terminal region sequence C R R Q E D S R V M V Y S A L R I P P E D |
polyclonal rabbit (liquid, affinity purified) |
IH, WB |
Aph-1aL |
human |
Aph-1aL/S Loop (poly)
|
Covance Research Products, Inc.
Cat# PRB-551P |
immunogen = human Aph-1aL/S Loop region 92-115 with seq C K L L K K A D E G L A S L S E D G R S P I S I R |
polyclonal rabbit (liquid, affinity purified) |
IH, WB |
Aph-1aL/S loop |
human |
Aph-1b C-Terminal (poly)
|
Covance Research Products, Inc.
Cat# PRB-552P |
immunogen = human Aph-1b/C-terminal region sequence C Q D K N F L L Y N Q R S R Q D K N F L L Y N Q R S R of human Aph-1b. |
polyclonal rabbit (liquid, affinity purified) |
IH, WB |
Aph-1b; some reactivity with Aph-1aL/S |
human |
Aph-1b Loop (poly)
|
Covance Research Products, Inc.
Cat# PRB-553P |
immunogen = human Aph-1b/loop region and recognizes the sequence C K L L K K A S E G L K S I N P G E T A P S M R |
polyclonal rabbit (liquid, BSA, glycerol, azide, affinity purified) |
IH, WB |
Aph-1b/loop region |
human |
APH-1L, O2C2 C-terminal (poly)
|
Covance Research Products, Inc.
Cat# PRB-550P |
immunogen = human Aph-
1aL/C-terminal region (epitope at CRRQEDSRVM VYSALRIPPED of
human Aph-1aL) |
polyclonal rabbit (liquid, affinity purified) |
IF, WB |
recognizes the C-terminal of human Aph-1aL |
human |
CSEN (2B3-A1) IgG1,κ
|
Novus Biologicals
Cat# H00030818-M01 supplied by Abnova only available in North America |
immunogen = full-length recombinant CSEN with GST tag |
monoclonal mouse (liquid, purified, no preservative) |
WB |
CSEN - calsenilin, presenilin binding protein, EF-hand transcription factor |
human |
Antigen (Clone) IgG |
Distributor (Producer) |
Immunogen (Epitope) |
Host (Formulation) |
Methods glossary |
Specificity |
Reactivity top |
CSEN (poly)
|
Novus Biologicals
Cat# H00030818-A01 supplied by Abnova only available in North America |
immunogen =
full-length recombinant CSEN with GST tag |
polyclonal mouse |
WB |
CSEN - calsenilin, presenilin binding protein, EF hand transcription factor |
human |
Nicastrin (poly) IgG
|
Abcam
cat# ab3444 |
immunogen = synthetic peptide (I N A K A D V L F I A P R E P G A V S Y corr. to C-terminal aa 688-708 from human nicastrin |
polyclonal rabbit (liquid, affinity purified, PBS, BSA, azide) |
WB |
~110 kDa nicastrin |
human, mouse (others not tested) |
Nicastrin (poly) IgG
|
Abcam
cat# ab24741 |
immunogen = synthetic peptide, conj. to KLH, corr. to N-terminal aa 103-124 of nicastrin |
polyclonal rabbit (liquid, affinity purified) |
IC, IF, IP, WB |
~85 kDa nicastrin |
human, mouse (others not tested) |
Nicastrin (poly)
|
Abnova Corporation
cat# H00023385-A01 |
immunogen = NCSTN (AAH47621, 16 a.a. ~ 115 a.a) partial recombinant protein with GST tag; GenBank Acc# BC047621 |
polyclonal mouse (liquid, glycerol) |
ELISA, WB |
Nicastrin |
|
Nicastrin (poly) IgG
|
A B R - Thermo Scientific
Cat# PA1-758 reference Lee, 2002 Gu, 2002 Puglielli, 2001 Deng, 2006 |
immunogen = synthetic peptide corr. to aa 688-708 of human nicastrin; one aa substitution between human and mouse sequence |
polyclonal rabbit (epitope affinity purified IgG, PBS, BSA, azide) |
WB |
Nicastrin in tissues and cells; ~110 kDa in WB |
human, mouse |
Nicastrin (poly) IgG
|
A B R - Thermo Scientific
Cat# PA1-86755 |
immunogen = a 18 amino acid peptide sequence near the internal region of human nicastrin |
polyclonal rabbit (liquid, PBS, azide) |
WB |
detects Nicastrin |
human, rat, mouse |
Nicastrin (poly) IgG
|
A B R - Thermo Scientific
Cat# PA1-86778 |
immunogen = a 17 amino acid peptide sequence near the carboxy terminus of human nicastrin |
polyclonal rabbit (liquid, PBS, azide) |
WB |
detects Nicastrin |
human, rat, mouse |
Nicastrin (poly) IgG
|
Acris Antibodies
Cat# SP5274P |
immunogen = synthetic peptide, C I N A K A D V L F I A P R E P G A V S Y, corresp. to aa 688-708 from human nicastrin |
polyclonal rabbit (liquid, PBS, BSA, azide, affinity purified) |
Neut, WB |
nicastrin (~110kDa) |
human, mouse |
Nicastrin (poly) IgG
|
Acris Antibodies
Cat# NB300-566 supplied by Novus |
immunogen = synthetic peptide, C I N A K A D V L F I A P R E P G A V S Y corresp. to aa 688-708 from human nicastrin |
polyclonal rabbit (liquid, PBS, BSA, azide) |
WB |
Nicastrin (~110kDa) |
human, mouse (others not tested) |
Nicastrin (poly) IgG
|
Acris Antibodies
Cat# NICN11-A supplied by Alpha Diagnostics |
immunogen = 19aa peptide from C-terminus of human nicastrin, conj. to KLH |
polyclonal rabbit (liquid, affinity purified) |
ELISA, IH, WB |
nicastrin (~80kDa) |
human (others not tested) |
Antigen (Clone) IgG |
Distributor (Producer) |
Immunogen (Epitope) |
Host (Formulation) |
Methods glossary |
Specificity |
Reactivity top |
Nicastrin (poly)
|
Acris Antibodies
Cat# NICN11-S supplied by Alpha Diagnostics |
immunogen = 19aa peptide from the C-terminus of human nicastrin conj to KLH |
polyclonal rabbit (liquid, unpurified serum) |
ELISA, IH, WB |
nicastrin (~80kDa) |
human (others not tested) |
Nicastrin (poly) IgG
|
A D I
Cat# NICN11-A |
immunogen = 19aa peptide from C-terminus of human nicastrin, conj. to KLH |
polyclonal rabbit (liquid, affinity purified) |
ELISA, IH, WB |
nicastrin (~80kDa) |
human (others not tested) |
Nicastrin (poly)
|
A D I
Cat# NICN11-S |
immunogen = 19aa peptide from the C-terminus of human nicastrin conj to KLH |
polyclonal rabbit (liquid, unpurified serum) |
ELISA, IH, WB |
nicastrin (~80kDa) |
human (others not tested) |
Nicastrin (poly) IgG
|
AMS Biotechnology
Cat# Q06730R supplied by Biodesign |
immunogen = synthetic peptide corr. to aa residues 689-709 at C-terminus of nicastrin |
polyclonal rabbit (liquid, protein-A purified, PBS, no preservative) |
IP, WB |
reacts with nicastrin protein |
human |
Nicastrin (poly) IgG
|
Autogen Bioclear
Cat# NICN11-A supplied by Alpha Diagnostics |
immunogen = 19aa peptide from C-terminus of human nicastrin, conj. to KLH |
polyclonal rabbit (liquid, affinity purified) |
ELISA, IH, WB |
nicastrin (~80kDa) |
human (others not tested) |
Nicastrin (poly)
|
Autogen Bioclear
Cat# NICN11-S supplied by Alpha Diagnostics |
immunogen = 19aa peptide from the C-terminus of human nicastrin conj to KLH |
polyclonal rabbit (liquid, unpurified serum) |
ELISA, IH, WB |
nicastrin (~80kDa) |
human (others not tested) |
Nicastrin (poly) IgG
|
Autogen Bioclear
cat# sc-14369 supplied by Santa Cruz |
immunogen = peptide mapping near the N-terminus of Nicastrin of human origin. |
polyclonal goat (liquid, PBS, azide, gelatin, affinity purified) |
IF, IP, WB |
120 and 145 kDa Nicastrin |
human, rat, mouse |
Nicastrin (poly) IgG
|
Autogen Bioclear
cat# sc-25648 supplied by Santa Cruz |
immunogen = epitope corresp. to aa 1-300 at the N-terminus of Nicastrin of human origin. |
polyclonal rabbit (liquid, PBS, azide, gelatin, affinity purified) |
IF, IP, WB |
120 and 145 kDa Nicastrin |
human, rat, mouse |
Nicastrin (poly) IgG
|
Axxora
Cat# ALX-210-561 |
immunogen = synthetic peptide corresp. to aa 688-708 (C I N A K A D V L F I A P R E P G A V S Y) of human nicastrin |
polyclonal rabbit (liquid, PBS, BSA, azide, affinity purified) |
WB |
nicastrin (~110kDa) |
human, mouse |
Nicastrin (poly)
|
Axxora
Cat# SIG-9370 supplied by Signet |
immunogen = synthetic peptide corresp. to aa 698-709 of human nicastrin |
polyclonal rabbit (frozen, PBS, purified, concentrated) |
IP, WB |
nicastrin |
human |
Antigen (Clone) IgG |
Distributor (Producer) |
Immunogen (Epitope) |
Host (Formulation) |
Methods glossary |
Specificity |
Reactivity top |
Nicastrin (poly)
|
Bachem
Cat# T-4789 |
Immunogen = aa 1-17 of human nicastrin |
polyclonal rabbit (purified antiserum) |
|
|
|
Nicastrin (poly)
|
Bachem
Cat# T-4788 |
Immunogen = aa 1-17 of human nicastrin |
polyclonal rabbit (diluted antiserum) |
RIA |
|
|
Nicastrin (poly)
|
Bachem
Cat# T-4787 |
Immunogen = aa 1-17 of human nicastrin |
polyclonal rabbit (undiluted antiserum) |
IH |
|
|
Nicastrin (poly)
|
Bachem
Cat# S-4790 |
Immunogen = aa 656-676 of human nicastrin |
polyclonal rabbit (undiluted antiserum) |
IH |
|
|
Nicastrin (poly)
|
Bachem
Cat# S-4791 |
Immunogen = aa 656-676 of human nicastrin |
polyclonal rabbit (diluted antiserum) |
RIA |
|
|
Nicastrin (kit)
|
Bachem
Cat# S-3189 |
Immunogen = aa 1-17 of human nicastrin |
rabbit |
IF |
|
|
Nicastrin (kit)
|
Bachem
Cat# S-2239 |
Immunogen = aa 1-17 of human nicastrin |
rabbit |
RIA |
|
|
Nicastrin (kit)
|
Bachem
Cat# S-1263 |
Immunogen = aa 656-676 of human nicastrin |
rabbit (high sensitivity) |
ELISA |
|
|
Nicastrin (kit)
|
Bachem
Cat# S-1262 |
Immunogen = aa 1-17 of human nicastrin |
rabbit (high sensitivity) |
ELISA |
|
|
Nicastrin (kit)
|
Bachem
Cat# S-3190 |
Immunogen = aa 656-676 of human nicastrin |
rabbit |
IF |
|
|
Antigen (Clone) IgG |
Distributor (Producer) |
Immunogen (Epitope) |
Host (Formulation) |
Methods glossary |
Specificity |
Reactivity top |
Nicastrin (kit)
|
Bachem
Cat# S-2240 |
Immunogen = aa 656-676 of human nicastrin |
rabbit |
RIA |
|
|
Nicastrin (kit) IgG
|
Bachem
Cat# S-4792 |
Immunogen = aa 656-676 of human nicastrin |
rabbit (purified antiserum) |
|
|
|
Nicastrin (poly) IgG
|
Biodesign Meridian LifeScience
Cat# Q06730R |
immunogen = synthetic peptide corr. to aa residues 689-709 at C-terminus of nicastrin |
polyclonal rabbit (liquid, protein-A purified, PBS, no preservative) |
IP, WB |
reacts with nicastrin protein |
human |
Nicastrin (poly) IgG
|
Biotrend
Cat# NICN11-A supplied by ADI(?) |
immunogen = 19aa peptide from C-terminus of human nicastrin, conj. to KLH |
polyclonal rabbit (IgG fraction, liquid, affinity purified) |
ELISA, IH, WB |
nicastrin (~80kDa) |
human |
Nicastrin (poly)
|
Biotrend
Cat# NICN11-S supplied by ADI(?) |
immunogen = 19aa peptide from the C-terminus of human nicastrin conj to KLH |
polyclonal rabbit (antiserum) |
ELISA, IH, WB |
nicastrin (~80kDa) |
human |
Nicastrin (poly) IgG
|
Calbiochem EMD Biosciences
Cat# 481905 |
immunogen = synthetic peptide corr. to aa residues 689-709 at C-terminus of nicastrin |
polyclonal rabbit (liquid, undiluted serum, conj. with KLH) |
IB, IP, RIA |
both the latent and active forms of Nicastrin |
human, mouse |
Nicastrin (poly) IgG
|
Calbiochem EMD Biosciences
Cat# 481906 |
immunogen = synthetic peptide corr. to aa residues 62-93 at N-terminus of nicastrin |
polyclonal rabbit (liquid, undiluted serum, conj. with KLH) |
IB, IF, IP |
both the letent and active forms of Nicastrin |
human, mouse |
Nicastrin (poly) IgG
|
CEDARLANE Laboratories
Cat# NICN11-A supplied by A D I |
immunogen = 19aa peptide from C-terminus of human nicastrin, conj. to KLH |
polyclonal rabbit (IgG fraction, liquid, affinity purified) |
ELISA, IH, WB |
nicastrin (~80kDa) |
human (others not tested) |
Nicastrin (poly)
|
CEDARLANE Laboratories
Cat# NICN11-S supplied by A D I |
immunogen = 19aa peptide from the C-terminus of human nicastrin conj to KLH |
polyclonal rabbit (antiserum) |
ELISA, IH, WB |
nicastrin (~80kDa) |
human (others not tested) |
Nicastrin (poly)
|
Cell Signaling
Cat# 3632 |
immunogen = synthetic peptide corr. to C-terminus of human Nicastrin, conj. to KLH |
polyclonal rabbit (affinity purified) |
WB |
endogenous levels of Nicastrin |
monkey, human, rat, mouse |
Antigen (Clone) IgG |
Distributor (Producer) |
Immunogen (Epitope) |
Host (Formulation) |
Methods glossary |
Specificity |
Reactivity top |
Nicastrin (mono) IgG2b
|
Chemicon and Upstate are Millipore
Cat# MAB5556 |
immunogen = synthetic peptide from mouse Nicastrin |
monoclonal mouse (liquid, ascites, no preservative) |
IC(C), IP, WB |
nicastrin |
human, rat, mouse (expect most mammals) |
Nicastrin (poly)
|
Chemicon and Upstate are Millipore
Cat# AB5890 |
immunogen = synthetic peptide corresponds to aa 690-708 of mouse nicastrin |
polyclonal guinea pig (Liquid, serum, no preserve) |
IH, WB |
Nicastrin in neural cell bodies in hippocampal formation, cerebral cortex, thalamic nuclei, Purkinje cells of the cerebellum, motoneurons in the brainstem and spinal cord |
rat (from homology expect human, mouse) |
Nicastrin (poly)
|
Chemicon and Upstate are Millipore
Cat# AB5944 Discontinued |
immunogen =
synthetic peptide from the C-terminus of human Nicastrin |
polyclonal rabbit (liquid, affinity purified) |
IH, WB |
nicastrin |
human (others not tested) |
Nicastrin (poly)
|
Covance Research Products, Inc.
Cat# PRB-365P |
immunogen = C-terminal fragment of nicastrin corresp. to a peptide containing residues 691-709 [C]-N A K A D V L F I A P R E P G A V S Y, conj. to KLH |
polyclonal rabbit (liquid, affinity purified) |
IF, IP, WB |
nicastrin |
human, mouse |
Nicastrin (poly)
|
Covance Research Products, Inc.
Cat# PRB-364P |
immunogen = N-terminal fragment of nicastrin corresp. to a peptide containing residues 103-124 (C F T R D L M E K L K G R T S R I A G L A V S L), coupled to KLH |
polyclonal rabbit (liquid, affinity purified) |
IF, IP, WB |
nicastrin |
human, mouse |
Nicastrin (poly) IgG
|
Covance Research Products, Inc.
Cat# SIG-39370 supplied by Signet |
immunogen = aa 698-709 of nicastrin |
polyclonal rabbit (liquid, purified, PBS, no carrier, no preservative) |
IP, WB |
nicastrin protein |
human |
Nicastrin (poly)
|
Euromedex
cat# H00023385-A01 supplied by Abnova |
immunogen = NCSTN (AAH47621, 16 a.a. ~ 115 a.a) partial recombinant protein with GST tag; GenBank Acc# BC047621 |
polyclonal mouse (liquid, glycerol) |
ELISA, WB |
nicastrin |
|
Nicastrin (poly)
|
Euromedex
Cat# N2525 supplied by US Biological |
immunogen = synthetic peptide from the C-terminus of human Nicastrin |
polyclonal rabbit (liquid, affinity purified) |
IH, WB |
Nicastrin (~100-130kDa and ~35kDa) |
human |
Nicastrin (poly)
|
Euromedex
Cat# N2525-06 supplied by US Biological |
immunogen = synthetic peptide (KLH coupled) corresp. to residues surrounding the C-terminus of human Nicastrin |
polyclonal rabbit (liquid, HEPES, NaCl, BSA, glycerol, affinity purified) |
WB |
endogenous levels of nicastrin |
human |
Nicastrin (poly) IgG
|
Euromedex
Cat# N2525-05 supplied by US Biological |
immunogen = synthetic peptide corresp. to the mouse Nicastrin protein |
polyclonal guinea pig (liquid, serum, no preservative) |
IH, WB |
nicastrin |
rat (expect human, mouse) |
Antigen (Clone) IgG |
Distributor (Producer) |
Immunogen (Epitope) |
Host (Formulation) |
Methods glossary |
Specificity |
Reactivity top |
Nicastrin (poly) IgG
|
Euromedex
Cat# N2525-10 supplied by US Biological |
immunogen = 19 aa synthetic peptide within the C-terminus of human nicastrin, coupled to KLH |
polyclonal rabbit (liquid, neat serum, glycerol) |
ELISA, WB |
nicastrin |
human |
Nicastrin (poly)
|
Euromedex
Cat# N2525-11 supplied by US Biological |
immunogen = 19 aa peptide within the C-terminus of human nicastrin, coupled to KLH |
polyclonal rabbit (liquid, PBS, azide, glycerol, affinity purified) |
ELISA, WB |
nicastrin |
human |