|
Antibodies to α-Synuclein and other synuclein Proteins
|
| From the Laboratories
|
Antigen
(Clone)
IgG |
Name
(Source)
used in |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
α-Synuclein
(mono)
|
α-Synuclein LB509
(Takeshi Iwatsubo, Department of Neuropathology & Neuroscience, Grad. School of Pharmaceutical Sciences, University of Tokyo)
Also see Abcam ab27766,
SantaCruz sc-58480, and others.
References:
Tokuda, 2010
Tsika, 2010
Xu, 2002 |
(epitope at the C terminus of the protein, residues 115-122) |
monoclonal mouse
|
IH, WB |
detects α-Synuclein, C-terminal |
human
(Paper: Baba M., et al., Amer. J. Pathol. 152:879-884,1998) |
α-Synuclein
(mono)
IgG
|
(Virginia Lee, University of Pennsylvania, CNDR, Philadelphia, PA)
used in paper by Kirik 2003 |
|
monoclonal mouse
|
IH |
|
|
α-Synuclein
(mono)
|
α-Synuclein LB509
(Virginia Lee, University of Pennsylvania, CNDR, Philadelphia, PA)
Reference: Giasson, 2002 |
immunogen = human α-synuclein |
monoclonal mouse
|
IH, WB |
react with human α-synuclein,
migrating at -18 kd, but nor cross with β-synuclein |
human
(Papers:
Giasson B I. Jakes R.. Goedert M. ... Lee V.M.-Y. J. Neurosci. Res., 59:528-533, 2000
Baba, et al. AmJ Pathol 152:879–884) |
α-Synuclein
(mono)
|
α-Synuclein Syn102, Syn202
(Virginia Lee, University of Pennsylvania, CNDR, Philadelphia, PA)
used in paper by Giasson, 2002 |
immunogen = human α- and β-synuclein |
monoclonal mouse
|
|
human α- and β-synuclein |
(reference:
Giasson B I. Jakes R.. Goedert M. ... Lee V.M.-Y. J. Neurosci. Res., 59:528-533, 2000) |
α-Synuclein
(mono)
|
α-Synuclein Syn505, Syn506, Syn514
(Virginia Lee, University of Pennsylvania, CNDR, Philadelphia, PA)
References:
Tsika, 2010
Giasson, 2002 |
generated using recombinant human α-synuclein oxidized/nitrated in vitro with peroxynitrite |
monoclonal mouse
|
IH, WB |
detect oxidized human α-synuclein; preferentially immunostain pathological α-syn compared to normal α-syn; complex conformational changes may be responsible for the observed antibody recognition patterns |
human, rat, mouse, canary
(reference papers:
Giasson BI. Duda JE. Murray IV. ... Lee V.M.-Y. Science., 290:985-989, 2000
Waxman, Giasson and Duda Acta Neuropathol. 2008 July; 116(1): 37–46.) |
α-Synuclein
(mono)
|
α-Synuclein nSyn823
(Virginia Lee, University of Pennsylvania, CNDR, Philadelphia, PA)
used in paper by Giasson, 2002 |
immunogen = nitrated α-synuclein |
monoclonal mouse
|
|
nitration specific α-synuclein |
(reference:
Giasson BI. Duda JE. Murray IV. ... Lee V.M.-Y. Science., 290:985-989, 2000) |
α-Synuclein
(mono)
|
α-Synuclein Syn303
(Virginia Lee, University of Pennsylvania, CNDR, Philadelphia, PA)
References:
Reference:
Tsika, 2010
Giasson, 2002 |
immunogen = oxidized α-synuclein |
monoclonal mouse
|
|
preferentially recognizes
oxidized and/or nitrated α-syn in human inclusions; recognizes oligomeric forms of α-syn in human brain tissue with α-syn aggregates |
human
(reference:
Giasson BI. Duda JE. Murray IV. ... Lee V.M.-Y. Science., 290:985-989, 2000) |
α-Synuclein
(mono)
|
α-Synuclein Syn211
(Virginia Lee, University of Pennsylvania, CNDR, Philadelphia, PA)
References:
Tsika, 2010
Giasson, 2002 |
immunogen = human α-synuclein (epitope is localized to amino acids 121-125 of human α-synuclein) |
monoclonal mouse
|
|
detects α-synuclein |
human
(reference:
Giasson B I. Jakes R.. Goedert M. ... Lee V.M.-Y. J. Neurosci. Res., 59:528-533, 2000) |
α-Synuclein
(mono)
|
α-Synuclein Syn204
(Virginia Lee, University of Pennsylvania, CNDR, Philadelphia, PA)
used in paper by Giasson, 2002 |
immunogen = human α-synuclein |
monoclonal mouse
|
|
|
(reference:
Giasson B I. Jakes R.. Goedert M. ... Lee V.M.-Y. J. Neurosci. Res., 59:528-533, 2000) |
α-Synuclein
(mono)
|
α-Synuclein Syn208
(Virginia Lee, University of Pennsylvania, CNDR, Philadelphia, PA)
References:
Tsika, 2010
Giasson, 2002 |
immunogen = full-length recombinant human α-synuclein |
monoclonal mouse
|
IB |
recognizes an epitope between amino acid residues 89-110 in human α-synuclein |
human
(reference:
Giasson B I. Jakes R.. Goedert M. ... Lee V.M.-Y. J. Neurosci. Res., 59:528-533, 2000) |
Antigen
(Clone)
IgG |
Name
(Source)
used in |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
α-Synuclein
(poly)
|
(Mark Cookson,, NIA at NIH)
used in paper by Sherer 2002 |
|
polyclonal rabbit
|
IC, WB |
|
|
α-Synuclein
(poly)
|
α-Synuclein SNL-1
(Virginia Lee, University of Pennsylvania, CNDR, Philadelphia, PA)
used in paper by Giasson, 1999 |
immunogen = synthetic peptides corresponding to amino acids 104-119 in α-syn |
polyclonal rabbit
|
|
|
(reference:
Giasson B I. Jakes R.. Goedert M. ... Lee V.M.-Y. J. Neurosci. Res., 59:528-533, 2000) |
α-Synuclein
(poly)
|
α-Synuclein SNL-4
(Virginia Lee, University of Pennsylvania, CNDR, Philadelphia, PA)
used in paper by Giasson, 1999 |
immunogen = a synthetic peptide corresponding to residues 2-12 of α-synuclein |
polyclonal rabbit
|
|
|
(reference:
Giasson B I. Jakes R.. Goedert M. ... Lee V.M.-Y. J. Neurosci. Res., 59:528-533, 2000) |
α-Synuclein phos Ser129
(mono)
|
11A5
(Tamie Chilcote, ActiveSite Pharmaceuticals, San Francisco, CA)
Reference:
Paleologou, 2010 |
immunogen = the peptide CAYEMPSEEGYQ, corresp. to aa 124-134, with a phosphorylated Serine 129 and a Cys residue added at the N terminus for coupling |
monoclonal mouse
|
ELISA, IA, IB, WB |
detects α-synuclein phosphotylated at Serine 129 |
human
(Paper, Anderson et al., 2006) |
α-Synuclein phos Ser87
(poly)
|
S87-P
(Hilal Lashel, The Swiss Federal Institute
of Technology-Lausanne)
Reference:
Paleologou, 2010 |
immunogen = a synthetic peptide that corresponds to amino acid residues 81–93 of human α-syn, with S87 being phosphorylated |
polyclonal rabbit
|
WB |
detects serine 87 phosphorylated forms (S87-P) of α-syn; no cross with unphosphorylated, S129A, or S129-P α-syn; detects a weak band at 14 kDa |
human, mouse |
α-Synuclein, C-term
(poly)
|
α-Synuclein, 116-131
(W.P. Gai, Department of Human Physiology and Centre for Neuroscience, Flinders University, Bedford Park, South Australia,
Reference:
Paleologou, 2010 |
immunogen = C-terminus of human a-synuclein (amino acids 116–131) |
polyclonal sheep
|
IB |
recognizes α-Synuclein, C-terminal |
human
(Paper, W.P. Gai, et al., J Neurochem. 1999 Nov;73(5):2093-100.) |
α-Synuclein, C-term
(poly)
|
α-Synuclein, 111-131
(W.P. Gai, Department of Human Physiology and Centre for Neuroscience, Flinders University, Bedford Park, South Australia |
immunogen = C-terminus of human a-synuclein (amino acids 111–131) |
polyclonal rabbit
|
IB |
recognizes α-Synuclein, in Lewy bodies and neural inclusions in brain sections from
Parkinson’s disease or MSA cases |
human
(Paper, W.P. Gai, et al., J Neurochem. 1999 Nov;73(5):2093-100.) |
β-Synuclein
(mono)
|
β-Synuclein Syn207
(Virginia Lee, University of Pennsylvania, CNDR, Philadelphia, PA)
used in paper by Giasson, 2002 |
|
monoclonal mouse
|
|
β-synuclein |
(reference:
Giasson B I. Jakes R.. Goedert M. ... Lee V.M.-Y. J. Neurosci. Res., 59:528-533, 2000) |
γ-Synuclein
(poly)
|
γ-Synuclein Syn
(Virginia Lee, University of Pennsylvania, CNDR, Philadelphia, PA)
used in paper by Giasson, 2002 |
|
polyclonal rabbit
|
|
γ-synuclein |
(reference:
Giasson BI. Duda JE. Forman MS. ... Lee V.M.-Y, Trojanowski JQ. Exp. Neurol., 172:354-362, 2001) |
| From the Manufacturers
|
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
αβ-synuclein
(syn 202)
IgG2a
|
Zymed/ Invitrogen
Cat# 32-8200
reference
Sherer 2002
reference
Cabin 2002 |
immunogen = recombinant human α- and β-synuclein |
monoclonal mouse
(affinity purified, liquid, PBS, azide) |
IF, IH(P), IP, WB |
both α-and β-synuclein; not γ-synuclein or synoretin |
human, mouse
(others not tested) |
α, β, γ-synuclein
(poly)
IgG
|
Autogen Bioclear
cat# sc-10717
produced by Santa Cruz |
immunogen = recombinant protein corresp. to a a 1-140 representing full length human α- β- and γ-synuclein |
polyclonal rabbit
(PBS, gelatin, azide) |
IF, IP, WB |
α-, β- and γ-synuclein |
human, rat, mouse |
α/ β/ γ-
Synuclein (FL-140)
(poly)
IgG
|
Santa Cruz
Cat# sc10717
Reference:
Tokuda, 2010 |
immunogen = recombinant protein corresp. to aa 1-140 representing full-length human α-synuclein |
polyclonal rabbit
(liquid, PBS, gelatin, azide) |
ELISA, IF, IP, WB |
recognizes α/ β/ γ-synuclein |
human, rat, mouse |
α/β-Synuclein
(Syn202)
IgG2a
|
Abcam
Cat# ab24715 |
immunogen = recombinant human α- and β- synuclein |
monoclonal mouse
(liquid, ascites, no preservative) |
IH(P), IP, WB |
recognises residues 130-140 in α- and β- synuclein |
human
(others not tested) |
α/β-Synuclein
(Syn 12)
IgG
|
Covance Research Products, Inc.
Cat# MMS 555R |
immunogen = nitrated alpha synuclein |
monoclonal mouse
(raw ascites fluid) |
IH, WB |
nitrated α/β-synuclein, but does not react with nonnitrated α-synuclein; recognizes nitration on either residues Y125 or Y136 in α-synuclein |
human |
α/β-Synuclein
(Syn 202)
IgG2a
|
Covance Research Products, Inc.
Cat# MMS 529R |
immunogen = recombinant alpha and beta synuclein |
monoclonal mouse
(raw ascites fluid) |
IH, IP, WB |
α- and β-synuclein, residues 130-140 |
human, rat, mouse, zebra, fish (various) |
α/β-Synuclein
(Syn202)
IgG2a
|
Santa Cruz
Cat# sc-32281 |
immunogen = recombinant human α/β-synuclein |
monoclonal mouse
(liquid, PBS, gelatin, azide]) |
IF, IP, WB |
α-synuclein and β-synuclein |
human, rat, mouse |
α/β-Synuclein
(poly)
IgG
|
Santa Cruz
Cat# sc7012 |
immunogen = peptide mapping at the N-terminus of human α-synuclein |
polyclonal goat
(liquid, PBS, gelatin, azide, affinity purified; blocking peptide [sc-7012]) |
IF, WB |
α-synuclein and β-synuclein |
human, rat, mouse |
α-Synuclein
(2B2D1 + 2B2A11)
|
Abcam
cat# ab36615 |
immunogen =
Ni-NTA purified truncated recombinant human alpha Synuclein expressed in E. coli strain BL21 (DE3)
|
monoclonal mouse
(liquid, ascites) |
ELISA, IH(F, P), WB |
α-Synuclein; cytoplasmic and nuclear; membrane bound in dopaminergic neurons |
human
(others not tested) |
α-Synuclein
(4B12)
IgG1
|
Abcam
Cat# ab1904 |
immunogen = recomb. human alpha synuclein, produced in E. coli |
monoclonal mouse
(liquid, precipitated and dialyzed supernate, PBS, azide) |
DB, ELISA, IH(F, P), WB |
α-Synuclein |
human
(others not tested, not mouse or rat) |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
α-Synuclein
(4D6)
IgG1
|
Abcam
Cat# ab1903 |
immunogen = recomb. human alpha synuclein, produced in E. coli |
monoclonal mouse
(liquid, precipitated and dialyzed supernate, PBS, no preservative) |
ELISA, IH(F, P), WB |
α-Synuclein |
human, rat, mouse
(others not tested) |
α-Synuclein
(LB509)
IgG1
|
Abcam
Cat# ab27766 |
immunogen = Lewy bodies purified from patients suffering dementia with Lewy bodies |
monoclonal mouse
(liquid, PBS, no preservative, protein G purified) |
ELISA, IH(F, P), WB |
α-Synuclein; epitope located in region encoded by aa 115-122 |
human
(others not tested, not rodent) |
α-Synuclein
(Syn12)
IgG
|
Abcam
Cat# ab24592 |
immunogen = full length protein human, nitrated synuclein
|
monoclonal mouse
(liquid, ascites, PBS, BSA, glycerol, azide, affinity purified) |
IH(P), WB |
reacts with nitrated α-synuclein (either Y125 or Y136) as well as β-synuclein but does not react with nonnitrated α-synuclein |
human
(others not tested) |
α-Synuclein
(syn204)
IgG2a,κ
|
Abcam
Cat# abB3309 |
immunogen = recomb. full length human alpha synuclein |
monoclonal mouse
(liquid, protein-A purified, PBS, BSA, azide) |
WB |
highly specific for α-synuclein and does not react with beta and gamma synucleins |
human
(others not tested not mouse or rat) |
α-Synuclein
(Syn514)
IgG
|
Abcam
Cat# ab24717 |
immunogen = full length protein human, oxidized alpha synuclein |
monoclonal mouse
(liquid, ascites, no preservative) |
IH(P), WB |
recognises native and nitrated/oxidized α-synuclein; some cross-reactivity with β and γ synucleins |
human
(others not tested) |
α-Synuclein
(poly)
IgG
|
Abcam
cat# ab6162 |
immunogen = synthetic peptide M P V D P D N E A Y E M P S E E (aa 116-131) of human α-synuclein coupled to diphtheria toxoid with a cysteine residue attached to the N-terminal of the peptide |
polyclonal sheep
(liquid, affinity purified) |
IH(F, P), WB |
alpha synuclein |
human, rat, mouse, rodent (various)
(others not tested) |
α-Synuclein
(poly)
IgG
|
Abcam
Cat# ab2080 |
immunogen = synthetic peptide (aa 116-131) on the C-terminus of human alpha synuclein peptide |
polyclonal goat
(whole antiserum, no preservative) |
ELISA, IH(P), WB |
α-Synuclein C-terminal |
human
(others not tested) |
α-Synuclein
(poly)
IgG
|
Abcam
Cat# ab15530 |
immunogen = synthetic peptide: G I L E D M P V D P D N E A Y E M P S E E, corresp. to C-terminal aa 111-131 of human alpha synuclein
|
polyclonal rabbit
(liquid, PBS, BSA, azide, affinity purified) |
IH(P) |
alpha Synuclein; not beta synuclein |
human, rat |
α-Synuclein
(poly)
IgG
|
Abcam
Cat# ab21976 |
immunogen = synthetic peptide: A K E G V V A A A E T K Q G V, corresp. to aa 11-26 of human alpha synuclein |
polyclonal sheep
(liquid, whole antiserum, filtered) |
IH(P), WB |
alpha Synuclein |
human |
α-Synuclein
(poly)
IgG
|
Abcam
Cat# ab15531 |
immunogen = synthetic peptide: G I L E D M P V D P D N E A Y E M P S E E, corresp. to C-terminal aa 111-131 of human alpha synuclein
|
polyclonal rabbit
(liquid, PBS, BSA, azide, affinity purified; prediluted) |
IH(P) |
alpha Synuclein; not beta synuclein |
human, rat |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
α-Synuclein
(poly)
IgG
|
Abcam
Cat# ab16784
Reference:
Clinton, 2010 |
immunogen = synthetic peptide: E A Y E M P S E E G Y Q D Y E P E A, corresp. to C- terminal aa 123-140 of alpha synuclein. |
polyclonal guinea pig
(liquid, whole antiserum, azide) |
IC, WB |
alpha Synuclein |
human, rat, mouse, cow
(other species not tested) |
α-Synuclein
(poly)
IgG
|
Abcam
cat# ab21975 |
immunogen =
synthetic peptide: A K E G V V A A A E K T K Q G V, corresp. to aa 11-26 of human α- Synuclein
|
polyclonal sheep
(liquid, filtered serum, no preservative) |
IH(P), WB |
α- Synuclein |
human
(expect mouse, rat chicken due to seq. homology; other species not tested) |
α-Synuclein
(syn204)
IgG2a
|
A B R - Thermo Scientific
Cat# MA1-21893 |
immunogen = recombinant full length human alpha-synuclein |
monoclonal mouse
(liquid,PBS, BSA, azide, protein A purified) |
WB |
α-Synuclein |
human |
α-Synuclein
(poly)
IgG
|
A B R - Thermo Scientific
Cat# PA1-21121 |
immunogen = synthetic peptide from human C-terminal aa sequence (commercially sensitive) |
polyclonal rabbit
(liquid, PBS, BSA, azide, affinity purified) |
IH(P) |
α-Synuclein |
human, rat |
α-Synuclein
(4D6)
|
Accurate
Cat #
YSRT-MAC2206 |
|
monoclonal mouse
|
ELISA, IH(F, P), WB |
α-Synuclein |
human, rat, mouse |
α-Synuclein
(KM51)
|
Accurate
Cat #
MED-CLA499 |
|
monoclonal mouse
(lyophilized, supernatant) |
IH(P); not WB |
α-Synuclein not β-Synuclein |
human |
α-Synuclein
(KM51)
|
Accurate
Cat #
MED-CLA891 |
|
monoclonal mouse
(supernatant) |
IH(P); not WB |
α-Synuclein not β-Synuclein |
human |
α-Synuclein
(mono)
IgG1
|
A D I
Cat# SYN11 |
immunogen = recombinant human synuclein-alpha protein |
monoclonal rabbit
(neat or affinity purified; control peptide available) |
ELISA, WB; IH?, IF? |
α-Synuclein |
human
(86% homology with mouse, rat and chicken. others not known) |
α-Synuclein
(poly)
|
A D I
Cat# SYN12 |
immunogen = 19 aa peptide locating near the C-terminus of human synuclein-alpha, form 2 |
polyclonal rabbit
(neat or affinity purified, control peptide) |
ELISA, IF, IH, IP, WB |
rat-α-synuclein form 2; not forms 1 or 3, and not β-Synuclein |
rat |
α-Synuclein
(kit)
|
Amersham GE Healthcare
Cat# RPN5902 |
|
|
ELISA |
specific for synuclein; measures whole protein and peptide fragment 117-131 |
|
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
α-Synuclein
(mono)
IgG2a,κ
|
AnaSpec
Cat# 53877 |
immunogen = human recombinant synuclein-α |
monoclonal mouse
(liquid, ascites, protein-A purified, PBS, BSA, azide) |
WB |
detects α-Synuclein |
human |
α-Synuclein
(mono)
IgG1
|
Autogen Bioclear
cat# sc-12767
produced by Santa Cruz |
immunogen = aa 121-125 of human α-synuclein |
monoclonal mouse
(liquid, PBS, gelatin, azide) |
IF, IP, WB |
α-synuclein |
human |
α-Synuclein
(syn204)
IgG2a
Autogen Bioclear
cat# ABJ1343 |
immunogen = human recombinant α-synuclein |
monoclonal mouse
(liquid, PBS, BSA, azide, Protein A purified) |
WB |
α-synuclein; not β- or γ-synuclein |
human
(not mouse or rat; other species not tested) |
|
α-Synuclein
(Syn204)
IgG2a
|
Autogen Bioclear
cat# sc-32280
produced by Santa Cruz |
immunogen = human recombinant α-synuclein |
monoclonal mouse
(liquid, PBS, gelatin, azide) |
IF, IP, WB |
α-synuclein |
human |
α-Synuclein
(poly)
IgG
|
Autogen Bioclear
cat# sc-7011
produced by Santa Cruz |
immunogen = C-terminus of human α-synuclein |
polyclonal goat
(liquid, PBS, azide, gelatin, affinity purified; blocking peptide [sc-7011P]; rabbit antibody [sc-7011R]) |
IF, IP, WB |
α-synuclein; no cross with β-synuclein |
human, rat, mouse |
α-Synuclein
(poly)
IgG
|
Autogen Bioclear
cat# sc-7012
produced by Santa Cruz |
immunogen = peptide mapping at the N-terminus of human α-synuclein |
polyclonal goat
(liquid, PBS, gelatin, azide, affinity purified; blocking peptide [sc-7012P]) |
IF, WB |
α-synuclein and β-synuclein |
human, rat, mouse |
α-Synuclein
(poly)
|
Autogen Bioclear
cat# SYN11S
affin. pur.
cat# SYN11A |
immunogen = 15 aa peptide near C-terminus of human α-synuclein |
polyclonal rabbit
(neat serum, thimerosal or merthiolate) |
ELISA, IF, IH(F), IP, WB |
α-synuclein |
human, rat, mouse |
α-Synuclein
(poly)
|
Autogen Bioclear
cat# SYN12-S
affin. pur.
cat# SYN12-A |
immunogen = 19 aa peptide near C-terminus of rat α-synuclein form 2 |
polyclonal rabbit
(neat serum, thimerosal or merthiolate) |
ELISA, IF, IH(F), IP, WB |
α-synuclein, form 2; not forms 1or 3; not β-synuclein |
rat, mouse |
α-Synuclein
(15G7)
rat IgG2a
|
Axxora
cat# ALX-804-258 References:
Neumann, 2006 |
immunogen = synthetic peptide corresp. to aa 116-131 of human α-synuclein |
monoclonal rat
|
IH(F, P), IP, WB |
α-synuclein; not recombinant β- or γ-synuclein. |
human
(not mouse) |
α-Synuclein
(4B12)
IgG1
|
Axxora
Cat# SIG-9730
produced by Signet |
immunogen = purified human α-synuclein produced in E. coli |
monoclonal mouse
(frozen) |
ELISA, IC, IH(F, P), WB |
α-synuclein; no cross with β- and γ-synucleins |
human
(not rat) |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
α-synuclein
(4D6)
IgG1
|
Axxora
Cat# SIG-9720
produced by Signet |
immunogen = purified human α-synuclein produced in E. coli |
monoclonal mouse
(frozen) |
ELISA, IH(F, P), WB |
α-synuclein; no cross with β- or γ-synucleins |
human, rat, mouse |
α-synuclein
(LB509)
IgG1
|
Axxora
Cat# SIG-9725
produced by Signet |
immunogen = synthetic peptide corresp. to aa 115-122 of human α-synuclein |
monoclonal mouse
|
ELISA, IH(F, P), WB |
α-synuclein |
human
(not mouse) |
α-Synuclein
(poly)
|
Biogenesis AdB Serotec
Cat# 84831000 |
aa 116-131 on the C-terminus ofhuman synt. αsynuclein |
polyclonal goat
(liquid, affinity purified, PBS, azide) |
ELISA, IH(P) |
aa 116-131 on the C-terminus ofhuman αsynuclein |
human |
α-Synuclein
(poly)
|
Biomol Enzo Life Sciences
Cat# SA3400 |
immunogen = a synthetic peptide corresponding to residues 117-131 of human α-synuclein. |
polyclonal rabbit
(liquid, PBS, azide) |
IH, IP, WB |
α-synuclein |
human, rat
(not mouse) |
α-Synuclein
(Syn211)
IgG1
|
Biosource Invitrogen
Cat# AHB0261 |
immunogen = recombinant human α-synuclein, aa 121-125 |
monoclonal mouse
(liquid, affinity purified IgG, PBS, BSA, azide) |
ELISA, IH(P), WB |
19 kDa protein, α-synuclein |
human
(not mouse or rat; others not tested) |
α-Synuclein
(poly)
|
Biosource Invitrogen
Cat# AHB0211 |
immunogen = synthetic peptide M P V D P D N E A Y E M P S E E corresp. to aa 116-131 of C-terminus of human α-synuclein. |
polyclonal goat
(liquid, whole serum, azide) |
ELISA, IH(P) |
the C-terminus of α-synuclein |
human
(others not tested) |
α-Synuclein
(poly)
|
Biosource Invitrogen
Cat# AHB0401 |
|
polyclonal rabbit
|
IH, WB |
α-synuclein |
human, rat |
α-Synuclein
(poly)
|
Calbiochem EMD Biosciences
Cat# 575000 |
aa. 116-131 (MPVDPDNE AYEMPSEE) of the human synt. α-synuclein protein, coupled to KLH |
polyclonal goat
(liquid, no preserv.) |
ELISA, IH(P) |
human a-synuclein protein |
human |
α-Synuclein
(poly)
|
Calbiochem EMD Biosciences
Cat# PC325 |
aa. 123-140 (EAYEMPSEE GYQDYEPEA) of the C-terminal of the cloned rat α-synuclein |
polyclonal guinea pig
(undiluted serum, azide) |
IF, IH(F), WB |
α-synuclein in the central nervous system |
human, rat |
α-Synuclein
(LB509)
IgG1,κ
|
Cambio
cat# 18-0215
produced by Zymed/Invitrogen |
immunogen = Lewy bodies purified from patients sufferingfrom DLB |
monoclonal mouse
(IHC Concentrate) |
IH(F, P), WB |
α-synuclein,
and not β-synucleinor γ-synuclein |
human |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
α-Synuclein
(poly)
|
Cell Signaling
Cat# 2642
reference
Xu, 2002 |
immunogen = syntheticpeptide (KLH coupled) derived from the C-terminal seq. of human α-synuclein |
polyclonal rabbit
(affinity purified, liquid, HEPES, BSA, glycerol) |
IP, WB |
alpha isoform (18 kDa) of synuclein; the antibody may cross-react with beta-synuclein |
monkey, human, rat, mouse |
α-Synuclein
(7B12.2)
IgG
|
Chemicon and Upstate are Millipore
cat# MAB5320 |
immunogen = recombinant human α-synuclein
|
monoclonal mouse
(liquid, filtered supernatant, no preservative) |
IH(F), WB |
α-synuclein; no reactivity with β- or γ-synuclein |
human
(not rat; others not tested) |
α-Synuclein
(Syn211)
|
Chemicon and Upstate are Millipore
cat# 36-008 |
immunogen = full-length recombinant human α-Synuclein
|
monoclonal mouse
(liquid, ascites, azide) |
IH, IP, WB |
α-synuclein (~14kDa) |
human, zebra fish
(not mouse or rat; others not tested) |
α-Synuclein
(poly)
|
Chemicon and Upstate are Millipore
cat# AB5038
References:
Clinton, 2010
Paleologou, 2010, both, unspecified formulation |
immunogen = peptide seq. N-G I L E D M P V D P D N E A Y E M P S E E C-C, corresp. to human α-synuclein a.a. 111-131 coupled to KLH |
polyclonal rabbit
(lyophilized, serum, no preservative) |
IC, IH(F, P), WB |
detects α-synuclein (14-19kDa); the immunizing peptide was chosen to reduce cross-reactivity of AB5038 with β-synuclein to a minimum |
human, rat
(others not tested) |
α-Synuclein
(poly)
|
Chemicon and Upstate are Millipore
cat# AB5038P
Reference:
Paleologou, 2010 |
immunogen = peptide seq. N-G I L E D M P V D P D N E A Y E M P S E E C-C corresp. to human α-synuclein a.a. 111-131coupled to KLH |
polyclonal rabbit
(Ig, affinity purified, lyophilized) |
IH(P), WB |
detects α-synuclein; (14-19kDa) |
human, rat
(others not tested ) |
α-Synuclein
(poly)
|
Chemicon and Upstate are Millipore
cat# AB5242 |
immunogen = a a. 116-131 on the C-terminus of human α-synuclein |
polyclonal goat
(serum, thimerosal) |
ELISA, IH(P) |
aa. 116-131 on the C-terminus of human α-synuclein |
human
(others not tested) |
α-Synuclein
(poly)
|
Chemicon and Upstate are Millipore
cat# AB5334P |
immunogen = a a. 116-131 of human α-synuclein |
polyclonal sheep
(Ig, affinity purified, lyophilized) |
IH(F, P), WB |
α-synuclein |
human, rat
(others not tested) |
α-Synuclein
(poly)
|
Chemicon and Upstate are Millipore
cat# AB5336P |
immunogen = synthetic peptide corresp. to a a. 108-120 of human α-synuclein |
polyclonal sheep
(Ig, affinity purified, lyophilized) |
IH(F, P) |
α-synuclein |
human, rat, mouse
(others not tested) |
α-Synuclein
(LB509)
IgG1
|
Covance Research Products, Inc.
Cat# SIG-39726 |
|
monoclonal mouse
(purified antibody, PBS; no preservatives or carrier proteins) |
DB, IH(F, P), WB |
LB509 recognizes amino acids 115-122 of human α-synuclein |
human
(not rodent) |
α-Synuclein
(Syn 204)
IgG2a
|
Covance Research Products, Inc.
Cat# MMS 530R |
immunogen = recombinant alpha synuclein |
monoclonal mouse
(raw ascites fluid) |
IH, IP, WB |
α-synuclein, residues 1-130 |
human |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
α-Synuclein
(Syn 514)
IgG
|
Covance Research Products, Inc.
Cat# MMS 556R |
immunogen = oxidized alpha synuclein |
monoclonal mouse
(raw ascites fluid) |
IH, WB |
recognizes native α-synuclein and nitrated/oxidized α-synuclein; some cross with β- and γ-synucleins |
human |
α-Synuclein
(poly)
|
EMD Biosciences, Inc.
Cat# PC325 |
immunogen = synthetic peptide (E A Y E M P S E E G Y Q D Y E P E A) aa. 123-140 of the C-terminal of the cloned rat α-synuclein |
polyclonal guinea pig
(liquid, serum) |
IF, IH(F), WB |
α-synuclein |
human, rat, mouse, bovine |
α-Synuclein
(poly)
|
EMD Biosciences, Inc.
Cat# 575000 |
immunogen = synthetic peptide (M P V D P D N E A Y E M P S E E) corresp. to aa 116-131 of human α-synuclein, conj. to KLH |
polyclonal goat
(liquid, neat serum, no preservative) |
ELISA, IH(P) |
α-synuclein |
human, rat, mouse |
α-Synuclein
(poly)
|
Fitzgerald
Cat# 20-SG70 |
immunogen = synthetic peptide equiv. to aa. 116-131 of C-terminal of PD human α-synuclein |
polyclonal goat
(Neat serum, no preservatives) |
ELISA, IH(P), WB |
C-terminus aa. 116-131 of α-synuclein |
human |
α-Synuclein
(4B12)
IgG1
|
GenWay Biotech
Cat# 20-272-192257 |
immunogen = recomb. human alpha-synuclein produced in E. coli. |
monoclonal mouse
(liquid, supernatant, purified, PBS, azide) |
DB, ELISA, IH, WB |
α-synuclein |
|
α-Synuclein
(4D6)
IgG1
|
GenWay Biotech
Cat# 18-783-71669 |
immunogen = recomb. human alpha-synuclein |
monoclonal mouse
(liquid, Ig fraction, purified, PBS, azide) |
ELISA, IH, WB |
α-synuclein |
rat, mouse |
α-Synuclein
(4D6)
IgG1
|
GenWay Biotech
Cat# 20-272-192256 |
immunogen = recomb. human alpha-synuclein produced in E. coli. |
monoclonal mouse
(liquid, supernatant, purified, PBS, no preservative) |
ELISA, IH, WB |
α-synuclein |
|
α-Synuclein
(poly)
|
GenWay Biotech
Cat# 18-783-76340 |
immunogen = synthetic peptide corresp. to aa 116-131 of the C-terminus of α-synuclein |
polyclonal goat
(liquid, serum, azide) |
ELISA, IH(P) |
α-synuclein in brain, spinal cord |
human |
α-Synuclein
(poly)
|
GenWay Biotech
Cat# 18-272-196445 |
immunogen = synthetic peptide corresp. to aa 111-131 near the C-terminus of human α-synuclein |
polyclonal rabbit
(liquid, PBS, BSA, azide, affinity purified) |
IH(P) |
α-synuclein; does not react with β-synuclein |
|
α-Synuclein
(poly)
|
GenWay Biotech
Cat# 18-272-196446 |
immunogen = synthetic peptide near the C-terminus of human α-synuclein |
polyclonal rabbit
(liquid, PBS, BSA, azide, affinity purified) |
IH(P) |
α-synuclein; does not react with β-synuclein |
|
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
α-Synuclein
(poly)
|
Li Starfish
cat# AFF-SA3400
produced by Affiniti |
synthetic peptide corresponding to residues [Cys],117-131of human α-synuclein |
polyclonal rabbit
(azide, purified) |
IB, IH |
brain-derived α-synuclein. |
human, rat
(rat to a lesser extent) |
α-Synuclein
(mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C26325 |
immunogen = recombinant full-length oxidized human α-Synuclein |
monoclonal mouse
(IgG fractionation) |
ELISA, IC, IF, IH, WB |
reacts with the nitrated/oxidized and non-nitrated forms of human α-Synuclein protein. Cross-reactivity with β- and γ-synucleins has not been observed in bacterially-expressed and purified recombinant proteins |
human
(95% seq. homology with mouse and rat) |
α-Synuclein
(mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C26329 |
immunogen = recombinant human α- and β-Synuclein |
monoclonal mouse
(protein A purified) |
ELISA, IC, IF, IH(P), WB |
α- and β- Synuclein; no crossreaction with γ-synuclein or synoretin |
human, mouse |
α-Synuclein
(mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C11640 |
immunogen = recombinant human α-Synuclein |
monoclonal mouse
(IgG fractionation) |
ELISA, IH(F, P), WB |
α-Synuclein |
human, rat, mouse |
α-Synuclein
(mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C26327 |
immunogen = recombinant human α-Synuclein |
monoclonal mouse
(protein A purified) |
ELISA, IH, IP, WB |
α-Synuclein; no cross with β- and γ-Synuclein or synoretin |
human |
α-Synuclein
(mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C26941 |
|
monoclonal mouse
|
ELISA, IH, WB |
α-Synuclein |
human |
α-Synuclein
(mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C26330 |
immunogen = full-length nitrated recombinant human α-synuclein |
monoclonal mouse
(ascites) |
IC, IH, WB |
recognizes recombinant, nitrated α-synuclein (14.5kD) or nitrated β--Synuclein (17kD) |
human |
α-Synuclein
(mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C26331 |
immunogen = full-length nitrated recombinant human α-synuclein |
monoclonal mouse
(ascites) |
IC, IH, WB |
recognizes recombinant, nitrated α-synuclein (14.5kD) or nitrated β--Synuclein (17kD) |
human |
α-Synuclein
(mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C26326 |
immunogen = purified E. coli produced human α-synuclein |
monoclonal mouse
(DEAE-column chromatography) |
ELISA, WB |
α-synuclein |
human |
α-Synuclein
(mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C26328 |
immunogen = Lewy Bodies purified from brains of patients suffering dementia with Lewy Bodies |
monoclonal mouse
(protein A purified) |
IH, WB |
α-synuclein; no crossreaction with β- and γ--synuclein |
human |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
α-Synuclein
(poly)
|
LifeSpan Biosciences, Inc.
Cat# LS-C26322 |
immunogen = synthetic peptide: corresp. to aa116-131, M P V D P D N E A Y E M P S E E |
polyclonal goat
|
ELISA, IH(F, P), WB |
α-Synuclein |
human |
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