|
Antibodies to Caspase-1,-2 and -3 proteins
|
| From the Manufacturers
|
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
Caspase-α, IN
(poly)
IgG
|
AnaSpec
Cat# 55369
Anti- Caspase-α (IN), Z-Fish |
Immunogen = synthetic peptide derived from the intermediate region of zebrafish caspase-α protein |
polyclonal rabbit
(liquid, affinity purified, MOPS buffer , BSA, glycerol) |
IH, WB |
recognizes Caspase-α |
zebra fish |
Caspase-β, IN
(poly)
IgG
|
AnaSpec
Cat# 55370
Anti- Caspase-β (IN), Z-Fish |
Immunogen = synthetic peptide derived from the intermediate region of zebrafish caspase-β protein |
polyclonal rabbit
(liquid, affinity purified, MOPS buffer , BSA, glycerol) |
IH, WB |
recognizes Caspase-β |
zebra fish |
Caspase-1
(poly)
IgG
|
AnaSpec
Cat# 53291 |
Immunogen = a
synthetic peptide from the second quarter of human Caspase 1 protein |
polyclonal rabbit
(liquid, affinity purified, PBS, BSA,azide) |
IH(P), IP, WB |
recognizes Caspase-1 |
human, rat, mouse |
Caspase-1 p-10 (C-20)
(poly)
IgG
|
Santa Cruz
Cat# sc-515
Reference:
Rao, 2008 |
immunogen = caspase-1 p10 of human origin (epitope at C-terminus) |
polyclonal rabbit
(liquid, affinity purified, PBS, gelatin, azide) |
ELISA, IF, IP, WB |
detects p10 subunit and precursor of caspase-1 of human origin; non cross-reactive with caspase p20 |
human |
Caspase-1, CT
(poly)
IgG
|
AnaSpec
Cat# 54209 |
Immunogen = a
16 amino acid peptide from near C-terminus of human caspase-1 |
polyclonal rabbit
(liquid, affinity purified, PBS, azide) |
ELISA, IH, WB |
recognizes Caspase-1 |
human |
Caspase-1, IN
(poly)
IgG
|
AnaSpec
Cat# 54210 |
Immunogen = a
15 amino acid peptide from near the internal sequence of human caspase-1 |
polyclonal rabbit
(liquid, affinity purified, PBS, azide) |
ELISA, IH, WB |
recognizes Caspase-1 |
human |
Caspase-10
(poly)
|
AnaSpec
Cat# 27007 |
Immunogen = a
synthetic peptide from C-terminus of human caspase 10 |
polyclonal rabbit
(liquid, affinity purified, PBS, BSA, azide) |
IH |
recognizes Caspase-10 |
human |
Caspase-10, CT
(poly)
IgG
|
AnaSpec
Cat# 53292 |
Immunogen = a
peptide corresponding to amino acids 505-521 of human FLICE2 |
polyclonal rabbit
(liquid, affinity purified, PBS, azide) |
ELISA, WB |
recognizes Caspase-10 |
human |
Caspase-14
(poly)
IgG
|
AnaSpec
Cat# 54109 |
Immunogen = a
16 amino acid peptide from near the C-terminus of human caspase-14 |
polyclonal rabbit
(liquid, affinity purified, PBS, azide) |
ELISA, IH, WB |
recognizes Caspase-14 |
human, rat, mouse |
Caspase-2
(18E809)
IgM
|
Abcam
Cat# ab13870 |
immunogen =
peptide corresp.to a portion of human Caspase 2
|
monoclonal mouse
(liquid, PBS, gelatin, azide) |
WB |
caspase-2 |
human
(expect mouse, rat) |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
Caspase-2
(3G63)
IgG2a
|
Abcam
Cat# ab18132 |
immunogen =
recombinant full length protein (human)
|
monoclonal mouse
(liquid, PBS, azide, protein A purified) |
ELISA, WB |
Caspase 2; no cross reaction with other proteins in caspase family |
human |
Caspase-2
(4i13)
IgG2a
|
Abcam
Cat# ab18029 |
immunogen =
synthetic peptide human C- terminal
|
monoclonal mouse
(liquid, ascites, HEPES, NaCl, BSA, glycerol, no preservative) |
WB |
detects endogenous levels of proCaspase 2 as well as its 14 and 12 kDa cleaved fragments |
human
(not mouse) |
Caspase-2
(B22)
IgG1
|
Abcam
Cat# ab18835 |
immunogen =
recombinant full length human Caspase 2
|
monoclonal mouse
(liquid, PBS, BSA, glycerol, azide, protein G purified) |
WB |
detects the 48 kDa of human procaspase-2 |
human |
Caspase-2
(Y154)
IgG
|
Abcam
Cat# ab32021 |
immunogen =
synthetic peptide corresp. to residues in the C-terminus of human Caspase 2 p18 subunit
|
monoclonal rabbit
(liquid, tris-glycine saline, BSA, glycerol, azide) |
IP, WB;
not FACS, IC, IH |
specific for the pro form and the p18 cleaved form of Caspase 2 |
human
(not mouse, rat) |
Caspase-2
(Y61)
IgG
|
Abcam
Cat# ab32435 |
immunogen =
synthetic peptide -corresp. to N-terminus of human Caspase-2 p15 subunit
|
monoclonal rabbit
(liquid, tris-glycine saline, BSA, glycerol, azide) |
FC, WB;
not IC, IP |
recognizes both pro-form (~50kDa) and
p15 cleaved-form of Caspase-2 |
human, mouse
(not rat) |
Caspase-2
(poly)
IgG
|
Abcam
Cat# ab407 |
immunogen =
synthetic C-terminal of human peptide
|
polyclonal rabbit
(liquid, whole serum) |
IC, IH, WB |
reacts with caspase-2 from cell extracts; recognizes the uncleaved form of Caspase 2
|
human
(other species not tested) |
Caspase-2
(poly)
IgG
|
Abcam
Cat# ab25896 |
immunogen =
synthetic peptide, consisting of 16 N-terminal aa of human Caspase 2
|
polyclonal rabbit
(liquid, PBS, azide, affinity purified) |
WB |
recognises Caspase-2
|
human, mouse |
Caspase-2
(poly)
IgG
|
Abcam
Cat# ab18737 |
immunogen=
synthetic peptide surrounding aa 417 of mouse caspase-2
|
polyclonal rabbit
(liquid, PBS, BSA, glycerol, thimerosal, affinity purified) |
WB |
detects the full length and small subunit of caspase 2; also detects a 30kDa intermediate fragment in human samples |
human, rat, mouse |
Caspase-2
(poly)
IgG
|
Abcam
Cat# ab22686 |
immunogen =
recombinant full length human protein
|
polyclonal rabbit
(liquid, whole serum, azide) |
WB |
recognises proenzyme (49 kDa), large (19 kDa) and small (12 kDa) subunits of mature enzyme; no cross-reactivity with other caspases tested (caspases 1, 3 to 10 and 14) |
human
(other species not tested) |
Caspase-2
(10C6)
IgG2a
|
Chemicon and Upstate are Millipore
Cat# MAB3501 |
immunogen: =
raised against the P19 subunit of recombinant human Caspase-2 protein |
monoclonal rat
(liquid, bronidox) |
ELISA, FC, IP, WB |
recognizes a reactive epitope that lies in the P19 subunit of human Caspase-2 (ICH-1, Nedd-2) |
monkey, human, mouse, dog
(other species not tested) |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
Caspase-2
(11B4)
IgG2a
|
Chemicon and Upstate are Millipore
Cat# MAB3507 |
immunogen =
raised against the P19 subunit of recombinant human Caspase-2 protein |
monoclonal rat
(liquid, bronidox) |
ELISA, FC, IP, WB |
recognizes a reactive epitope that lies in the P19 subunit of human Caspase-2 (ICH-1, Nedd-2) |
monkey, human, mouse, dog |
Caspase-2
(kit)
|
Chemicon and Upstate are Millipore
Cat# APT163 |
|
(Colorimetric assay, VDVAD) |
|
|
mammal (various) |
Caspase-2
(kit)
|
Chemicon and Upstate are Millipore
Cat# APT162
Discontinued |
|
(Fluorometric assay, VDVAD) |
|
|
mammal (various) |
Caspase-2
(mono)
IgG
|
Epitomics
Cat# 1060-1 |
Immunogen = synthetic peptide corresponding to N-terminus of hCaspase-2 p15 subunit |
monoclonal rabbit
(liquid, tris-glycine, azide, glycerol) |
FC, WB |
Caspase-2 (pro-form and p15 cleaved form) |
human |
Caspase-2
(mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C12462
available in USA only |
immunogen =
recombinant human caspase-2 (p19 subunit)
|
monoclonal rat
(protein G purified) |
ELISA, FC, IC, IH(F) |
caspase-2 p19 subunit |
monkey, human, rat, mouse, dog |
Caspase-2
(mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C12454
available in USA only |
immunogen =
recombinant full length human Caspase-2 protein
|
monoclonal mouse
(protein A purified) |
ELISA, WB |
caspase-2; no cross-reaction with other proteins in the caspase family |
human |
Caspase-2
(mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C12459
available in USA only |
immunogen =
synthetic peptide corresp. to the C-terminal portion of human caspase-2
|
monoclonal mouse
(ascites) |
ELISA, WB |
detects endogenous levels of human procaspase-2 as well as its 14 and 12kD cleaved fragments |
human |
Caspase-2
(mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C12464
available in USA only |
immunogen =
His-tagged p19 fragment of recombinant human caspase-2
|
monoclonal rat
(protein G purified) |
IP, WB |
caspase-2 (p19); detects bands of ~51kDa (procaspase-2), ~32kDa and ~18kDa (cleavage products) on WB |
monkey, human, rat, mouse, dog |
Caspase-2
(mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C21297
available in USA only |
immunogen =
a synthetic peptide corresp. to N-terminus of human Caspase-2 p15 subunit |
monoclonal rabbit
(supernatant) |
FC, WB |
Caspase-2 p15 subunit
|
human, rat, mouse |
Caspase-2
(mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C21298
available in USA only |
immunogen =
a synthetic peptide corresp. to C-terminus of human Caspase-2 p18 subunit |
monoclonal rabbit
(supernatant) |
IP, WB |
Caspase-2 p18 subunit
|
human |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
Caspase-2
(mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C505 |
immunogen =
synthetic peptide corresp. to a portion of human Caspase-2
|
monoclonal mouse
(liquid, PBS, gelatin, azide) |
WB |
caspase 2 |
human |
Caspase-2
(mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C12456
available in USA only |
immunogen =
recombinant protein of full-length human caspase-2 |
monoclonal rabbit
|
WB |
Caspase-2 and a 48 kD pro-caspase-2 |
human |
Caspase-2
(mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C12457
available in USA only |
immunogen =
recombinant human caspase-2 |
monoclonal rabbit
(protein G purified) |
WB |
caspase-2 long (48kDa) and short forms |
human |
Caspase-2
(mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C12458
available in USA only |
immunogen = synthetic peptide corresp. to a portion of human Caspase-2 |
monoclonal rabbit
(ascites) |
WB |
caspase-2 long (48kDa) form |
human, rat, mouse |
Caspase-2
(mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C12463
available in USA only |
immunogen =
recombinant human Caspase-2 (32-169aa) purified from E. coli
|
monoclonal rat
(protein G purified) |
ELISA, WB |
caspase 2 (ICH, Nedd2) |
human |
Caspase-2
(poly)
|
LifeSpan Biosciences, Inc.
Cat# LS-C18764 |
immunogen =
N-terminus of rat Caspase-2 protein through a cysteine residue link, conjugated to KLH |
polyclonal rabbit
(protein G purified) |
ELISA, FC, IF |
caspase-2 |
rat, mouse |
Caspase-2
(poly)
|
LifeSpan Biosciences, Inc.
Cat# LS-C461 |
immunogen =
synthetic 16 aa peptide from the N-terminus of human Caspase-2 |
polyclonal rabbit
(liquid, PBS, azide) |
IC, WB |
caspase-2 |
human, mouse |
Caspase-2
(poly)
|
LifeSpan Biosciences, Inc.
Cat# LS-C26865 |
|
polyclonal rabbit
|
IH, WB |
caspase-2 |
human, mouse |
Caspase-2
(poly)
|
LifeSpan Biosciences, Inc.
Cat# LS-C12461
available in USA only |
immunogen =
synthetic peptide derived from the C-terminal portion of human caspase-2
|
polyclonal rabbit
(protein A purified) |
ELISA, WB |
recognizes caspase 2 pro form and the small subunit of active caspase 2 |
human, rat, mouse, chicken |
Caspase-2
(poly)
|
LifeSpan Biosciences, Inc.
Cat# LS-C644 |
immunogen =
synthetic peptide of human Caspase-2
|
polyclonal rabbit
(liquid, PBS, azide, protein G purified) |
WB |
caspase 2 |
human |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
Caspase-2
(poly)
|
LifeSpan Biosciences, Inc.
Cat# LS-C12455
available in USA only |
immunogen =
synthetic peptide of human caspase-2 (aa residues 15-27)
|
polyclonal rabbit
(protein A purified) |
WB |
detects 50, 34 and 30kD proteins, corresponding to the two procaspase 2 isoforms and the intermediate cleavage fragments (pro-domain and large subunit) |
human, rat, mouse |
Caspase-2
(poly)
|
LifeSpan Biosciences, Inc.
Cat# LS-C12460
available in USA only |
immunogen =
synthetic peptide, M A A S S G R S Q S S L H R K G L C corresp. to the N-terminus of rat Caspase-2 protein (KLH) through a cysteine residue linker
|
polyclonal rabbit
(DEAE-column chromatography) |
ELISA |
caspase 2 long and short forms |
rat, mouse
(not human) |
Caspase-2
(poly)
|
LifeSpan Biosciences, Inc.
Cat# LS-C12465
available in USA only |
immunogen =
synthetic peptide of human caspase-2
|
polyclonal rabbit
|
WB |
caspase 2, cleaved |
human |
Caspase-2
(18E809.3)
IgM, κ
|
Novus Biologicals
Cat# NB100-56686 |
|
monoclonal mouse
(liquid, ascites) |
WB |
Caspase-2 |
human
(not tested in other species) |
Caspase-2
(Y154)
IgG
|
Novus Biologicals
Cat# NB110-55656 |
immunogen =
C-terminus of human Caspase-2 p18 subunit
|
monoclonal rabbit
(liquid, tris-glycine saline, glycerol, BSA, azide) |
IP, WB |
recognize both pro-form (~50kDa) and p18 cleaved-form of Caspase-2; predicted to detect the splice isoform (35kDa) based on seq. homology |
human
(notmouse or rat; other species not tested) |
Caspase-2
(Y61)
IgG
|
Novus Biologicals
Cat# NB110-55655 |
immunogen =
N-terminus of human Caspase-2 p15 subunit
|
monoclonal rabbit
(liquid, tris-glycine saline, glycerol, BSA, azide) |
FC, WB |
recognize both pro-form (~50kDa) and p15 cleaved-form of Caspase-2 |
human, mouse
(not rat; other species not tested) |
Caspase-2
(poly)
|
Novus Biologicals
Cat# H00000835-A01
supplied by Abnova
available in North America only
|
immunogen = partial recombinant CASP2 |
polyclonal mouse
(liquid, purified, no preservative) |
WB |
CASP2 - caspase 2, apoptosis-related cysteine protease (neural precursor cell expressed, developmentally down-regulated 2)
|
human
(other species not tested) |
Caspase-2
(poly)
IgG
|
Novus Biologicals
Cat#NB 100-1867
|
immunogen = synthetic human C-terminal peptide from CASP2 |
polyclonal rabbit
(lyopholized, sera, purified) |
IC, IH, WB |
CASP2 - caspase 2 from cell extracts and uncleaved form |
human
(other species not tested) |
Caspase-2
(poly)
IgG
|
Novus Biologicals
Cat#NB 100-56013
|
|
polyclonal rabbit
(lyopholized, sera, affinity purified) |
IC, IF, WB |
CASP2 - caspase 2 |
human, mouse
(other species not tested) |
Caspase-2
(poly)
IgG
|
Novus Biologicals
Cat# NB100-56689 |
|
polyclonal rabbit
(liquid, affinity purified) |
WB |
Caspase-2 |
human
(not tested in other species) |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
Caspase-2
(35)
IgG1
|
BD Pharmingen
Cat# 611023 150μg
Cat# 611022 50μg |
|
monoclonal mouse
(liquid, BSA, glycerol, azide, purified) |
IF, WB |
Caspase-2 |
human |
Caspase-2
(G310-1248)
IgG1
|
BD Pharmingen
Cat# 551093 50μg
Cat# 551094 150μg |
Immunogen = recombinant full-length hCaspase-2 (short form) |
monoclonal mouse
(liquid ascites, buffer, BSA, glycerol, azide, affinity purified) |
WB |
Caspase-2 (Ich-1) long (48kDa) and short (35kDa) forms |
human |
Caspase-2
(G310-1248)
IgG1
|
BD Pharmingen
Cat# 554131 |
Immunogen = recombinant full-length hCaspase-2 (short form) |
monoclonal mouse
(liquid, azide, affinity purified) |
WB |
Caspase-2 (Ich-1) long (48kDa) and short (35kDa) forms |
human |
Caspase-2
(poly)
Ig
|
BD Pharmingen
Cat# 552786 |
Immunogen = partially processed form of human caspase-2 |
polyclonal rabbit
(serum in buffer, azide) |
WB |
Caspase-2 (pro-form 48kDA and cleaved form (15kDa) |
human, rat, mouse |
Caspase-2
(mono)
IgG2b
|
Santa Cruz
Cat# sc-53928 |
immunogen =
amino acids 31-169 of human Caspase 2 |
monoclonal mouse
(liquid, PBS, gelatin, azide) |
WB |
Caspase 2
|
human
(other species not tested) |
Caspase-2
(mono)
IgG1
|
Santa Cruz
Cat# sc-56045 |
immunogen = full length human Caspase 2 |
monoclonal mouse
(liquid, PBS, gelatin, azide) |
WB |
Procaspase 2 |
human |
Caspase-2
(poly)
IgG1
|
Santa Cruz
Cat# sc-623 |
immunogen = peptide mapping at the N-terminus of human Caspase 2 |
polyclonal rabbit
(liquid, PBS, gelatin, azide, affinity purified; blocking peptide sc-623P) |
IF, IH(P), WB |
detects caspase-2 propeptide, caspase-2L and caspase-2S |
human, rat, mouse |
Caspase-2
(1C10)
IgG2b
|
AbD Serotec
Cat# MCA2563 |
immunogen = recombinant human caspase-2 (32-169aa) purified from E.coli
|
monoclonal mouse
(liquid, PBS, azide, protein G purified) |
ELISA, WB |
recognises recombinant caspase-2, a 53 kDa member of the cysteine-aspartic acid protease (caspase) family |
human |
Caspase-2
(kit)
|
AbD Serotec
Cat# ICT918 25 test
Cat# ICT919 100 test |
apoptosis detection
|
|
IF;
FLICA, FAM
fluorochrome inhibitor |
detects active caspases |
|
Caspase-2
(35)
IgG1
|
BD Transduction
Cat# 611022/3 |
Immunogen = human caspase-2 (Ich-1) long form |
monoclonal mouse
(liquid buffer, BSA, glycerol, azide, purified) |
IF, WB |
Caspase-2 (Ich-1L) |
human |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
Caspase-2
(Y61)
IgG
|
Upstate and Chemicon are Millipore
Cat# 04-571 |
immunogen =
synthetic peptide corresp. to N-terminus of human Caspase-2 p15 subunit |
monoclonal rabbit
(liquid, purified, Tris, saline, BSA, glycerol, azide) |
FC, WB |
Caspase 2; should recognize both pro-form (~50kDa) and p15 cleaved-form of Caspase-2
|
human, mouse |
Caspase-2 active
(poly)
IgG
|
Abcam
Cat# ab2251 |
immunogen =
synthetic peptide derived from within residues 300-400 of human Caspase 2, conjugated to KLH
|
polyclonal rabbit
(liquid, PBS, BSA, azide, affinity purified) |
ELISA |
recognises C-terminal aspartate D316 to distinguish the activated Caspase 2 |
human, rat, mouse |
Caspase-2, IN
(poly)
IgG
|
AnaSpec
Cat# 55368
Anti- Caspase-2 (IN), Z-Fish |
Immunogen = synthetic peptide derived from the intermediate region of zebrafish caspase-2 protein |
polyclonal rabbit
(liquid, affinity purified, MOPS buffer , BSA, glycerol) |
IH, WB |
recognizes Caspase-2 |
zebra fish |
Caspase-2, NT
(poly)
IgG
|
AnaSpec
Cat# 54205 |
Immunogen = a
16 amino acid peptide from the N-terminus of human caspase-2 |
polyclonal rabbit
(liquid, affinity purified, PBS, azide) |
ELISA, IC, WB |
recognizes Caspase-2 |
human, mouse |
Caspase-2, NT
(poly)
IgG
|
AnaSpec
Cat# 55367
Anti- Caspase-2 (NT), Z-Fish |
Immunogen = synthetic peptide derived from the N-terminal region of
zebrafish caspase-2 protein |
polyclonal rabbit
(liquid, affinity purified, MOPS buffer , BSA, glycerol) |
IH, WB |
recognizes Caspase-2 |
zebra fish |
Caspase-2L
(mono)
IgG1
|
Santa Cruz
Cat# sc-5292 |
immunogen = aa 317-435 mapping at the C-terminus of Caspase 2 |
monoclonal mouse
(liquid, PBS, gelatin, azide) |
IF, IP, WB |
detection of the p13 subunit, the full length caspase-2 precursor and caspase-2L |
human
(to a lesser extent, mouse and rat) |
Caspase-2L
(poly)
IgG
|
Santa Cruz
Cat# sc-626
Cat# sc-626G (goat) |
immunogen = peptide mapping at the C-terminus of mouse Caspase 2L |
polyclonal rabbit
(liquid, PBS, gelatin, azide, affinity purified; blocking peptide sc-626P) |
IF, IH(P), IP, WB |
detects the p13 subunit, the p12 subunit, full length caspase-2, and caspase-2L |
human, rat, mouse |
Caspase-2L
(poly)
IgG
|
Santa Cruz
Cat# sc-625
Cat# sc-625G (goat) |
immunogen = peptide mapping at the C-terminus of human Caspase 2L |
polyclonal rabbit
(liquid, PBS, gelatin, azide, affinity purified; blocking peptide sc-625P; conj. agarose sc-625AC) |
IF, IP, WB |
detects the p13 subunit, the p12 subunit, full length caspase-2, and caspase-2L; no cross-reaction with caspase-2S or the p18 subunit |
human |
Caspase-2L
(poly)
IgG
|
Santa Cruz
Cat# sc-8985 |
immunogen = aa 317-435 mapping at the C-terminus of Caspase 2 |
polyclonal rabbit
(liquid, PBS, gelatin, azide) |
IF, IP, WB |
detection of the p12 subunit, the p13 subunit, the full length caspase-2 precursor and caspase-2L |
human, rat, mouse |
Caspase-2S
(poly)
IgG
|
Santa Cruz
Cat# sc-15379 |
immunogen = epitope corresp. to aa 153-297 of human Caspase 2 |
polyclonal rabbit
(liquid, PBS, gelatin, azide) |
IF, IP, WB |
detection of full length caspase-2 precursor, the p18 subunit, caspase-2S and caspase 2L ; no cross-reaction with p12 subunit or the p13 subunit |
human, rat, mouse |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
Caspase-2L
(poly)
IgG
|
Biolegend
Cat# 634001 (125 μl)
cat# 634002 (500 μl) |
immunogen = peptide mapping to the C-terminal of human caspase-2L |
polyclonal rabbit
(affinity purified, PBS, gelatin, azide) |
IH(P), IP, WB |
detects the caspase-2L p12 subunit and precursor (also designated Ich-1L) |
human, rat, mouse |
Caspase-2L
(poly)
IgG
|
Novus Biologicals
Cat# NB120-7979 |
immunogen = synthetic peptide corresp. to the C-terminal of human caspase 2L |
polyclonal rabbit
(liquid, PBS, azide, gelatin, purified) |
IH, IP, WB |
caspase-2L p12 subunit |
human, rat, mouse
(other species not tested) |
Caspase-3
(31A893)
IgG1
|
Abcam
Cat# ab13586 |
immunogen =
recombinant fusion protein (6xHis tagged full-length human Caspase 3
|
monoclonal mouse
(liquid, PBS, azide, protein G purified) |
WB |
detects pro caspase 3, testing positive in NIH 3T3 cell lysate |
human |
Caspase-3
(3CSP03)
IgG2a
|
Abcam
Cat# ab2171 |
immunogen =
recombinant full length human protein
|
monoclonal mouse
(liquid, PBS, azide, protein A purified) |
IH(F, P), IP, WB |
Caspase 3 (32kDa) |
human |
Caspase-3
(3F48)
IgG1,κ
|
Abcam
Cat# ab17863 |
immunogen =
recombinant full length human protein
|
monoclonal mouse
(liquid, PBS, BSA, azide, protein G purified) |
IP, WB |
caspase-3 |
human
(other species not tested) |
Caspase-3
(3F49)
IgG2a
|
Abcam
Cat# ab17868 |
immunogen =
recombinant full length human protein
|
monoclonal mouse
(liquid, PBS, BSA, azide, protein A purified) |
IH(P), IP, WB |
Caspase 3 |
human
(other species not tested) |
Caspase-3
(3G2)
IgG1
|
Abcam
Cat# ab17815 |
immunogen =
synthetic peptide D S G I S L D N S Y K M D Y P E M, corresp. to aa 28-44 of human Caspase 3
|
monoclonal mouse
(liquid, HEPES, NaCl, BSA, glycerol) |
WB |
detects endogenous levels of full length (35kDa) and large fragment (17/19kD) of caspase 3 resulting from cleavage at Asp175 |
human
(expect mouse, rat, rabbitm cat chicken, dog and pig) |
Caspase-3
(4-1-18)
IgG2a
|
Abcam
Cat# ab18328 |
immunogen =
recombinant full length human protein
|
monoclonal mouse
(liquid, PBS, azide, affinity purified, biotinylated) |
WB |
Caspase-3
|
human
(expect mouse, rat, rabbit, chimp, cat, dog, pig, chinese hamster) |
Caspase-3
(4H334)
IgG
|
Abcam
Cat# ab17819 |
immunogen =
synthetic peptide of human N- terminal residues adjacent to Asp175, conjugated to KLH; epitode = between aa 161-175
|
monoclonal rabbit
(liquid, supernatant, HEPES, NaCl, BSA, glycerol, no preservative) |
IC, IP, WB |
detects endogenous levels of full length (35kDa) and large fragment (17/19kD) of caspase 3 resulting from cleavage at Asp175 |
human |
Caspase-3
(4i29)
IgG2a
|
Abcam
Cat# ab17862 |
immunogen =
N-terminal 6HIS tagged fusion protein corresp. to full length human Caspase 3
|
monoclonal mouse
(liquid, tris-glycine saline, azide, protein G purified) |
DB, ELISA |
recognizes full-length Caspase-3 at 32kDa and 17kDa subunit of 10ng recombinant
|
human
(not dog) |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
Caspase-3
(CPP32 4-1-18)
IgG2a,κ
|
Abcam
Cat# ab7850 |
immunogen =
recombinant full length human protein
|
monoclonal mouse
(liquid, ascites, azide) |
WB |
Caspase-3 |
human
(not mouse, rat) |
Caspase-3
(E61)
IgG
|
Abcam
Cat# ab32150 |
immunogen =
synthetic peptide corresp. to N-terminal residues of human pro-Caspase-3.
|
monoclonal rabbit
(liquid, tris-glycine saline, BSA, glycerol, azide) |
FC, IC, IF, IH(P), IP, WB |
recognizes the pro-form of Caspase-3 only; no reaction with the cleaved forms (active enzyme) of Caspase-3 |
human, mouse
(not rat; other species not tested) |
Caspase-3
(E83-103)
IgG
|
Abcam
Cat# ab32499 |
immunogen =
synthetic peptide corresp. to residues following Ser29 of human caspase-3 (N-terminus of p17 subunit).
|
monoclonal rabbit
(liquid, tris-glycine saline, BSA, glycerol, azide, protein A purified) |
FC, IC, IH(P), IP, WB |
detects pro-form (35kD) of caspase 3 only; does not recognize any cleaved caspases |
human, mouse
(not rat; other species not tested) |
Caspase-3
(E83-77)
IgG
|
Abcam
Cat# ab32042 |
immunogen = synthetic peptide corresp. to residues following Ser29 of human Caspase 3 (N terminus of p17 subunit).
|
monoclonal rabbit
(liquid, tris-glycine saline, BSA, glycerol, azide) |
IC, IF, IH(F, P), WB;
not FACS or IP |
detects the active (cleaved) form of Caspase 3 only; does not recognize the pro form of Caspase 3 |
human, mouse
(not rat; other species not tested) |
Caspase-3
(E87)
IgG
|
Abcam
Cat# ab32351 |
immunogen =
synthetic peptide corresp. to residues from within the first 100 aa in the p17 subunit of human Capase 3
|
monoclonal rabbit
(liquid, tris-glycine saline, BSA, glycerol, azide) |
IC, IH(P), IP, WB;
not FACS |
pro form and the p17 cleaved form of Caspase 3 |
human, rat
(not mouse; other species not tested) |
Caspase-3
(T46L)
IgG1
|
Abcam
Cat# ab2300 |
immunogen =
full length mouse protein
|
monoclonal mouse
(liquid, BSA, glycerol, azide) |
WB |
Caspase 3 (32 kDa) |
rat, mouse |
Caspase-3
(poly)
IgG
|
Abcam
Cat# ab408 |
immunogen = synthetic human peptide |
polyclonal rabbit
(liquid, whole antisera) |
IH(P), WB |
caspase 3 (32 kDa) |
human
(other species not tested) |
Caspase-3
(poly)
IgG
|
Abcam
Cat# ab4104 |
immunogen =
recombinant full length human protein
|
polyclonal rabbit
(liquid, whole antisera, azide) |
IH(P), WB |
Caspase 3 protease 32kDa, the 17kDa cleavage fragment but not the 11kDa fragment |
human
(other species not tested) |
Caspase-3
(poly)
IgG
|
Abcam
Cat# ab7980 |
immunogen =
synthetic human peptide (N terminal)
|
polyclonal rabbit
(liquid, PBS, azide, affinity purified) |
IH(P), IP, WB |
caspase-3 p20 subunit and precursor (also designated CPP32) |
human
(other species not tested) |
Caspase-3
(poly)
IgG
|
Abcam
Cat# ab1080 |
immunogen =
synthetic peptide, R G T E L D C G I E T D, corresp. to internal sequence aa 164-175 of human Caspase 3
|
polyclonal goat
(liquid, BSA, azide, affinity purified) |
WB |
caspase-3 |
human
(expect rat, mouse, dog, pig, sheep, hamster) |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
Caspase-3
(mono)
IgG2a
|
AnaSpec
Cat# 53295 |
Immunogen = a
recombinant full-length caspase-3 protein |
monoclonal mouse
(liquid, protein A purified, PBS, BSA,azide) |
IH(P), IP, WB |
recognizes Caspase-3 |
human |
Caspase-3
(19)
IgG2a
|
Beckman Coulter, Inc.
Cat# IM2120 |
|
monoclonal mouse
(liquid, purified) |
IH(F, P), WB |
Caspase 3 |
human |
Caspase-3
(4-1-18)
IgG2a,κ
|
Biolegend
Cat# 622703 (25 μg)
Cat# 622704 (100 μg) |
immunogen = recombinant full length human protein |
monoclonal mouse
(affinity purified, PBS, azide, Biotin conj.) |
WB |
recognizes 32 kD pro-caspase-3 and 17 kD cleaved caspase-3 |
human |
Caspase-3
(4-1-18)
IgG2a,κ
|
Biolegend
Cat# 622701 (25 μg)
Cat# 622702 (100 μg) |
immunogen = recombinant full length human protein |
monoclonal mouse
(affinity purified, PBS, azide) |
WB |
recognizes 32 kD pro-caspase-3 and 17 kD cleaved caspase-3 |
human |
Caspase-3
(poly)
IgG
|
Biolegend
Cat# 634101 (152 μl)
Cat# 634102 (500 μl) |
immunogen = peptide mapping to the N-terminal domain of human caspase-3 prescursor |
polyclonal rabbit
(affinity purified, PBS, gelatin, azide) |
IH, IP, WB |
detects the caspase-2L p12 subunit and precursor (also designated Ich-1L) |
human |
Caspase-3
(kit)
|
Cell Technology
Cat# APO Active 3 PE |
immunogen = aa 163-175 of murine caspase-3 |
rabbit
(PE) |
FC, IF |
caspase-3
(protocol) |
human, rat, mouse |
Caspase-3
(kit)
|
Cell Technology
Cat# APO Active 3 |
immunogen = aa 163-175 of murine caspase-3 |
rabbit
|
FC, IF |
caspase-3
(protocol) |
human, mouse |
Caspase-3
(poly)
|
Chemicon and Upstate are Millipore
Cat# AB3640 |
immunogen =
peptide corresp. to a.a. 104-117 of human Caspase 3 |
polyclonal rabbit
(liquid, PBS, azide, affinity purified) |
WB |
recognizes a region within the p17 large subunit of Caspase 3; detects both pro- and large subunit forms, as well as partially processed intermediates in apoptotically induced cells |
human, mouse
(other species not tested) |
Caspase-3
(kit)
|
Chemicon and Upstate are Millipore
Cat# APT165 |
|
(Colorimetric activity assay, DEVD) |
|
|
mammal (various) |
Caspase-3
(kit)
|
Chemicon and Upstate are Millipore
Cat# APT131 |
|
(Colorimetric activity assay, DEVD) |
|
|
mammal (various) |
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