|
Antibodies to Cathepsin proteins
|
| From the Manufacturers
|
Antigen (Clone) IgG |
Distributor (Producer) |
Immunogen (Epitope) |
Host (Formulation) |
Methods glossary |
Specificity |
Reactivity top |
Cathepsin B (CB131) IgG2b
|
Abcam
Cat# ab26395 |
immunogen = recombinant full length protein (human)
|
monoclonal mouse (liquid, supernatant, azide) |
IH(P); not IH(F) or WB |
Cathepsin B |
human (others not tested) |
Cathepsin B (poly) IgG
|
Abcam
Cat# ab33538 |
immunogen = recombinant rat pro Cathepsin B1 |
polyclonal rabbit (liquid, tris-glycine saline, glycerol; protein A purified) |
IH, WB |
recognizes Procathepsin B (~40kDa) and mature Cathepsin B (25kD, 26kD and 30kD |
rat, mouse (others not tested) |
Cathepsin B (poly) IgG
|
Abcam
Cat# ab30443 |
immunogen = full length native protein (purified): Cathepsin B from human liver |
polyclonal rabbit |
ELISA, WB |
recognises liver Cathepsin B; no cross-reaction with Cathepsin L or Cathepsin H. |
human (others not tested) |
Cathepsin B (poly) IgG
|
Abcam
Cat# ab211 |
immunogen = human Cathepsin B purified from liver
|
polyclonal sheep (liquid, EACA, benzamidine, glycine buffered saline, EDTA) |
WB |
recognises human Cathepsin B, a lysosomal cysteine protease of 29kD |
human (others not tested) |
Cathepsin B (poly) IgG
|
Abcam
Cat# ab35255 |
immunogen = full length native purified protein from human placenta
|
polyclonal sheep (liquid, PBS, azide; conj. FITC) |
IF |
Cathepsin B |
human (expect others; others not tested) |
Cathepsin B (poly) IgG
|
Abcam
Cat# ab35258 |
immunogen = full length native human purified Cathepsin B protein from placenta
|
polyclonal sheep (liquid, PBS, no preservative; conj. HRP) |
ELISA |
Cathepsin B |
human (expect others; others not tested) |
Cathepsin B (CB131) IgG2b
|
A B R - Thermo Scientific
Cat# MA1-23148 |
immunogen = recombinant full length human protein |
monoclonal mouse (liquid, supernatant, azide) |
IH(P) |
cathepsin B |
human |
Cathepsin B (CB131)
|
Accurate
Cat# MEDCLA962-01 |
|
monoclonal mouse (supernatant, lyophilized) |
IH(P); not WB or IH(F) |
|
human |
Cathepsin B (CB131)
|
Accurate
Cat# MEDCLA962-1 |
|
monoclonal mouse (supernatant, lyophilized) |
IH(P); not WB or IH(F) |
|
human |
Cathepsin B (poly)
|
Accurate
Cat# YSRTAHP18H |
|
polyclonal sheep (purified) |
ID, WB |
|
human |
Antigen (Clone) IgG |
Distributor (Producer) |
Immunogen (Epitope) |
Host (Formulation) |
Methods glossary |
Specificity |
Reactivity top |
Cathepsin B (poly)
|
Accurate
Cat# YSRTAHP591 |
|
polyclonal rabbit |
IH(F, P) |
|
human |
Cathepsin B (CB131) IgG2b
|
Acris Antibodies
Cat# SM2106P |
immunogen = recombinant human cathepsin B |
monoclonal mouse (lyophilized, supernatant, azide) |
IH(P); not IH(F) or WB |
Cathepsin B |
human |
Cathepsin B (poly)
|
Acris Antibodies
Cat# SP2043P |
|
polyclonal rabbit (liquid,PBS, BSA, azide, affinity purified) |
IH(F, P) |
Cathepsin B, precursor and mature forms, also enzyme/inhibitor complexes |
human |
Cathepsin B (poly)
|
Acris Antibodies
Cat# SP1127P |
immunogen = human cathepsin B purified from liver |
polyclonal sheep (liquid, GBS, EACA, EDTA, benzamidine, azide, purified) |
IP, WB |
cathepsin B |
human |
Cathepsin B (poly)
|
Acris Antibodies
Cat# BP332HRP |
immunogen = native cathepsin B from placenta |
polyclonal sheep (liquid, PBS; conj. to HRP) |
ELISA |
cathepsin B |
human, various species |
Cathepsin B (poly)
|
Acris Antibodies
Cat# BP332AP |
immunogen = highly pure cathepsin B from human placenta |
polyclonal sheep (liquid, PBS, glycerol, ZnCl2, MgCl2, azide; conj. AP) |
ELISA |
Cathepsin B |
human, various species |
Cathepsin B (poly)
|
Acris Antibodies
Cat# BP332F |
immunogen = highly pure cathepsin B from human placenta |
polyclonal sheep (liquid, PBS, azide; conj. FITC) |
IF |
cathepsin B |
human, various species |
Cathepsin B (poly)
|
Acris Antibodies
Cat# BP332 |
immunogen = highly pure cathepsin B from human placenta |
polyclonal sheep (liquid, PBS, azide) |
ELISA |
cathepsin B |
human, various species |
Cathepsin B
|
American Qualex
Cat# R3480 |
|
|
|
|
human |
Cathepsin B (CB59-4B11) IgG1
|
Axxora
Cat# ALX-804-431 |
immunogen = recombinant human procathepsin B, epitope (E212PGYSP217) of human cathepsin B. |
monoclonal mouse (liquid, PBS, thimerosal, protein G-affinity purified) |
IH(P), WB |
Recognizes mature human single or two chain cathepsin B and
procathepsin B. Does not cross-react with mature cathepsins H, K, L, S, V, W or procathepsins L, S, W. |
human |
Antigen (Clone) IgG |
Distributor (Producer) |
Immunogen (Epitope) |
Host (Formulation) |
Methods glossary |
Specificity |
Reactivity top |
Cathepsin B (kit)
|
Axxora
Cat# ICT-937-T025 and ICT-937-T100 |
|
(Magic Redâ„¢-(RR)2; substrate-based assay) |
FM |
Cathepsin B |
|
Cathepsin B (poly)
|
Biomol Enzo Life Sciences
Cat# SA-361 |
|
polyclonal rabbit (liquid, serum, azide) |
ELISA, WB |
Cathepsin B not cathepsins D, H or L |
human |
Cathepsin B (poly)
|
Biotrend
Cat# 1910-8304 |
Immunogen = highly pure Cathepsin B, from human placenta |
polyclonal sheep (Ig fraction, PBS, HRP conj) |
ELISA |
|
human (expect other species) |
Cathepsin B (poly)
|
Biotrend
Cat# 1910-8204 |
Immunogen = highly pure Cathepsin B, from human placenta |
polyclonal sheep (Ig fraction, PBS, azide, FITC conj) |
ELISA |
|
human (expect other species) |
Cathepsin B (poly)
|
Biotrend
Cat# 1910-8104 |
Immunogen = highly pure Cathepsin B, from human placenta |
polyclonal sheep (Ig fraction, PBS, azide, glycerol, ZnCl2, MgCl2, AP conj) |
ELISA; perhaps IB, IH |
|
human (expect other species) |
Cathepsin B (poly)
|
Biotrend
Cat# 1910-8004 |
Immunogen = highly pure Cathepsin B, from human placenta |
polyclonal sheep (Ig fraction, PBS, azide) |
ELISA; perhaps IB, IH |
|
human (expect other species) |
Cathepsin B (kit)
|
Biotrend
Cat# LC1161(A) |
|
(SCEA Red-(RR)2) |
FC, IF |
|
|
Cathepsin B (kit)
|
Biotrend
Cat# LC1161 |
|
(SCEA Red-(RR)2) |
FC, IF |
|
|
Cathepsin B (poly)
|
BioVision
Cat# 3190-100 |
immunogen = purified cathepsin B from human liver |
polyclonal rabbit (liquid, PBS, gelatin, thimerosal, affinity purified) |
IH, WB |
cathepsin B (pro and mature forms) |
human |
Cathepsin B (kit)
|
BioVision
Cat# K140-100 |
|
|
fluorescent assay |
cathepsin B |
|
Antigen (Clone) IgG |
Distributor (Producer) |
Immunogen (Epitope) |
Host (Formulation) |
Methods glossary |
Specificity |
Reactivity top |
Cathepsin B (CA10) IgG2a
|
Calbiochem EMD Biosciences
Cat# IM27L |
immunogen = cathepsin B purified from human liver; epitope within the heavy chain of mature cathepsin B |
monoclonal mouse (lyophilized, ammonium bicarbonate buffer, BSA) |
IF, IP, WB |
cathepsin (~43kDa latent and ~31kDa active forms) |
human, porcine, bovine (not rat) |
Cathepsin B (poly) IgG
|
Calbiochem EMD Biosciences
Cat# PC41 |
immunogen = mature human cathepsin B purified from liver |
polyclonal rabbit (liquid, PBS, gelatin, azide) |
IH(P), WB |
Cathepsin B (~43kDa latent and ~31kDa active forms) |
human |
Cathepsin B (poly) IgG
|
Calbiochem EMD Biosciences
Cat# 219408 |
immunogen = purified cathepsin B from human liver |
polyclonal rabbit (liquid, PBS, azide, purified) |
ELISA, WB |
cathepsin B; not cathepsin D, H or L |
human |
Cathepsin B (kit)
|
Cell Technology
Cat# CDK100-1/2 catalog online at website |
|
(substrate-based assay, cell permeable, fluorogenic, lyophilized) |
IF(C) |
|
|
Cathepsin B (poly)
|
Chemicon and Upstate are Millipore
Cat# AB4064 |
immunogen = Synthetic peptide corresponding to aa 92-108 of human cathepsin B. |
polyclonal rabbit (liquid, affinity purified, PBS, azide) |
WB |
recognizes the precursor
(~45kDa) and both mature forms of cathepsin B (~28kDa, 30kDa) |
human (others not tested) |
Cathepsin B (poly)
|
Chemicon and Upstate are Millipore
Cat# 06-480 supplied by Upstate |
immunogen = recomb. rat procathepsin B 1 |
polyclonal rabbit (liquid, Tris, azide) |
IH, WB |
recognizes procathepsin B (40kDa) and mature cathepsin B (25kDa, 26kDa and 30kDa) |
rat, mouse (others not tested) |
Cathepsin B (poly) IgG
|
Fitzgerald
Cat# CR6009RP |
immunogen = purified liver cathepsin-B |
polyclonal rabbit (liquid, PBS, azide) |
ELISA, WB |
cathepsin B; no cross-reaction with cathepsin H or L |
human |
Cathepsin B (poly)
|
Fitzgerald
Cat# 20-CR71 |
immunogen = cathepsin B from human liver |
polyclonal rabbit (liquid, PBS, azide) |
ELISA, IB |
cathepsin B; no cross reaction with cathepsin D, L or H |
human |
Cathepsin B (3E1) IgG2a
|
GeneTex
Cat# GTX75751 |
|
monoclonal mouse (liquid, PBS, azide, purified IgG) |
IH, WB |
cathepsin B (precursor and mature form) |
human (others not tested) |
Cathepsin B (CB131) IgG2b
|
GeneTex
Cat# GTX46395 |
immunogen = full length human protein |
monoclonal mouse (liquid, supernatant, azide) |
IH |
cathepsin B |
human (others not tested) |
Antigen (Clone) IgG |
Distributor (Producer) |
Immunogen (Epitope) |
Host (Formulation) |
Methods glossary |
Specificity |
Reactivity top |
Cathepsin B (CB131) IgG2b
|
GeneTex
Cat# GTX80410 |
immunogen = recombinant human cathepsin B |
monoclonal mouse (lyophilized, supernatant, azide) |
IH |
cathepsin B |
human (others not tested) |
Cathepsin B (poly) IgG
|
GeneTex
Cat# GTX20211 supplied by Abcam |
immunogen = human Cathepsin B purified from liver
|
polyclonal sheep (liquid, EACA, benzamidine, glycine buffered saline, EDTA) |
WB |
recognises human Cathepsin B, a lysosomal cysteine protease of 29kD |
human (others not tested) |
Cathepsin B (poly)
|
GeneTex
Cat# GTX74392 |
|
polyclonal rabbit (liquid, purified) |
IH |
cathespin B (mature and prescursor forms) |
human |
Cathepsin B (poly)
|
GeneTex
Cat# GTX74143 |
immunogen =
human cathepsin B purified from liver |
polyclonal sheep (liquid, glycine buffered saline, EDTA, EACA, benzamidine) |
ID, WB |
caathepsin B |
human |
Cathepsin B (CB131) IgG2b
|
GenWay Biotech
Cat# 20-783-71977 |
immunogen = recombinant human cathepsin B |
monoclonal rabbit (lyophilized, supernatant, azide) |
IH(P) |
cathepsin B |
human |
Cathepsin B (poly) IgG
|
GenWay Biotech
Cat# 18-783-76190 |
immunogen = human cathepsin B purified from liver |
polyclonal sheep (liquid, glycine buffered saline, azide, EACA, benzamidine, EDTA, purified) |
WB |
cathepsin B |
human |
Cathepsin B (poly)
|
ISL (Immune Systems Limited)
Cat# 126-0553 |
immunogen = native human cathepsin B |
polyclonal sheep (alkaline phosphate conj) |
|
|
human |
Cathepsin B (poly)
|
ISL (Immune Systems Limited)
Cat# 126-0552 |
immunogen = native human cathepsin B, from placenta |
polyclonal sheep (purified Ig) |
|
|
human |
Cathepsin B (poly)
|
ISL (Immune Systems Limited)
Cat# 126-0554 |
immunogen = native human cathepsin B, from placenta |
polyclonal sheep (FITC conj) |
|
|
human |
Cathepsin B (poly)
|
ISL (Immune Systems Limited)
Cat# 126-0555 |
immunogen = native human cathepsin B, from placenta |
polyclonal sheep (HRP conj) |
|
|
human |
Antigen (Clone) IgG |
Distributor (Producer) |
Immunogen (Epitope) |
Host (Formulation) |
Methods glossary |
Specificity |
Reactivity top |
Cathepsin B (CB131)
|
Kamiya
Cat# MC-130 |
immunogen = prokaryotic recombinant protein corresp. to the full length mature human cathepsin B molecule |
monoclonal mouse (lyophilized, supernatant, azide) |
IH(P) |
cathepsin B |
human |
Cathepsin B (kit)
|
Kamiya
Cat# KT-088 |
|
|
FM |
cathepsin B |
|
Cathepsin B (kit)
|
Leinco Technologies
Cat# C137 |
|
(SCEA Red-(RR)2) |
|
|
|
Cathepsin B (kit)
|
Leinco Technologies
Cat# C1161 |
|
(SCEA Red-(RR)2) |
IF |
|
|
Cathepsin B (mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C21362 |
immunogen = recombinant human procathepsin B
|
monoclonal mouse (protein G purified) |
IH, WB |
recognizes mature human single or two chain cathepsin B and procathepsin B; no crossreaction with mature cathepsins H, K, L, S, V, W or procathepsins L, S, W |
human |
Cathepsin B (mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C21361 |
immunogen = recombinant human cathepsin B
|
monoclonal mouse (supernatant) |
IH |
cathepsin B |
human |
Cathepsin B (poly)
|
LifeSpan Biosciences, Inc.
Cat# LS-C21357 |
immunogen = mature form cathepsin B, purified from human liver
|
polyclonal rabbit (DEAE-column chromatography) |
IH, WB |
cathepsin B pro- and mature forms |
human |
Cathepsin B (poly)
|
LifeSpan Biosciences, Inc.
Cat# LS-C21359 |
immunogen = recombinant rat pro-cathepsin B
|
polyclonal rabbit (protein A purified) |
IH, WB |
procathepsin B (~40kD) and mature cathepsin B (25kD, 26kD and 30kD). |
rat, mouse |
Cathepsin B (poly)
|
LifeSpan Biosciences, Inc.
Cat# LS-C21360 |
immunogen = human liver cathepsin B
|
polyclonal rabbit (protein A purified) |
ELISA, WB |
Cathepsin B; no crossreaction with Cathepsin L, Cathespin D or Cathepsin H
|
human |
Cathepsin B (poly)
|
LifeSpan Biosciences, Inc.
Cat# LS-C11270 available in USA only |
immunogen = native highly pure cathepsin B from human placenta
|
polyclonal sheep (ammonium sulfate purification) |
ELISA |
Cathepsin B
|
human |
Antigen (Clone) IgG |
Distributor (Producer) |
Immunogen (Epitope) |
Host (Formulation) |
Methods glossary |
Specificity |
Reactivity top |
Cathepsin B (poly)
|
LifeSpan Biosciences, Inc.
Cat# LS-C21358 available in USA only |
immunogen = human cathepsin B purified from liver |
polyclonal sheep (Ion exchange chromatography) |
WB |
Cathepsin B
|
human |
Cathepsin B (poly) IgG1
|
MBL International
Cat# JM-3190-100 |
|
polyclonal rabbit (liquid, PBS, gelatin, azide, affinity purified) |
IH, WB |
cathepsin B (pro and mature forms) |
human |
Cathepsin B (kit)
|
MBL International
Cat# JM-K140-100 |
|
|
Fluorescent activity assay |
Cathepsin B |
|
Cathepsin B (CB 59-4B11)
|
MoBiTec GmbH
Cat# 3012LS |
|
monoclonal |
|
|
human |
Cathepsin B (poly)
|
Neuromics
Cat# GT15046 |
Immunogen = recomb. human Cathepsin B |
polyclonal goat (liquid, affinity purified, PBS, Trehalose) |
ELISA, WB |
|
human |
Cathepsin B (poly)
|
Neuromics
Cat# GT15047 |
Immunogen = recomb. mouse Cathepsin B |
polyclonal goat (liquid, affinity purified, PBS, Trehalose) |
ELISA, IH, WB |
|
mouse |
Cathepsin B (CB131) IgG2b
|
Novocastra
Cat# NCL-CATH-B |
immunogen = prokaryotic recombinant protein corresp. to the full length mature human cathepsin B molecule |
monoclonal mouse (lyophilized, supernatant, azide) |
IH(P); not WB |
cathepsin B |
human |
Cathepsin B (CB131) IgG2b
|
Novus Biologicals
Cat# NB600-1111 |
immunogen = recombinant cathepsin B |
monoclonal mouse (liquid, PBS, azide) |
IH(P); not WB or IH(F) |
specific for cathepsin B |
human (others not tested) |
Cathepsin B (poly) IgG
|
Novus Biologicals
Cat# NB100-62439 |
immunogen = highly pure cathepsin B from human placenta |
polyclonal sheep (liquid, antisera, azide, alkaline phosphatase conj.) |
ELISA |
specific for cathepsin B |
human (others not tested) |
Cathepsin B (poly) IgG
|
Novus Biologicals
Cat# NB100-62187 |
|
polyclonal sheep (liquid, purified IgG, azide) |
ID, WB |
specific for cathepsin B |
human (others not tested) |
Antigen (Clone) IgG |
Distributor (Producer) |
Immunogen (Epitope) |
Host (Formulation) |
Methods glossary |
Specificity |
Reactivity top |
Cathepsin B (poly) IgG
|
Novus Biologicals
Cat# NB100-62440 |
immunogen = highly pure cathepsin B from human placenta |
polyclonal sheep (liquid, antisera, PBS, HRP conj.) |
ELISA |
specific for cathepsin B |
human (expect other species) |
Cathepsin B (poly) IgG
|
Novus Biologicals
Cat# NB100-64436 |
|
polyclonal sheep (liquid, serum, azide, FITC conj.) |
IF |
specific for cathepsin B |
human (others not tested) |
Cathepsin B (155714) IgG1
|
R&D Systems
Cat# MAB2176 |
immunogen = purified, NS0-derived, recombinant human cathepsin B |
monoclonal mouse (lyophilized, PBS, trehalose, protein G purified) |
IP |
cathepsin B |
human |
Cathepsin B (173317) IgG1
|
R&D Systems
Cat# MAB965 |
immunogen = purified, NS0-derived, recombinant mouse cathepsin B, aa 18-339 |
monoclonal rat (lyophilized, PBS, trehalose, protein G purified) |
WB |
Cathepsin pro and active forms |
human, mouse |
Cathepsin B (poly) IgG
|
R&D Systems
Cat# AF953 |
immunogen = NS0-derived human cathepsin B |
polyclonal goat (lyophilized, PBS, trehalose, affinity purified) |
ELISA, IH, IP, WB |
cathepsin B |
human |
Cathepsin B (poly) IgG
|
R&D Systems
Cat# BAF953 |
immunogen = purified, NS0-derived, recombinant human cathepsin B |
polyclonal goat (lyophilized, PBS, BSA, affinity purified, biotinylated) |
IH, WB |
cathepsin B |
human |
Cathepsin B (poly) IgG
|
R&D Systems
Cat# AF965 |
immunogen = purified, NS0-derived, recombinant mouse cathepsin B aa 18-30 |
polyclonal goat (lyophilized, PBS, trehalose, affinity purified) |
ELISA, IH, Neut, WB |
cathepsin B |
mouse |
Cathepsin B (poly) IgG
|
R&D Systems
Cat# BAF965 |
immunogen = purified, NS0-derived recombinant mouse cathepsin B, aa 18-339 |
polyclonal goat (lyophilized, PBS, trehalose, affinity purified; biotinylated) |
IH, WB |
cathepsin B |
mouse |
Cathepsin B (poly) IgG
|
Santa Cruz
Cat# sc-6490 |
immunogen = peptide mapping at the C-terminus of human cathepsin B |
polyclonal goat (liquid, PBS, gelatin, azide, affinity purified) |
IF, WB |
cathepsin B |
human, rat, mouse |
Cathepsin B (poly) IgG
|
Santa Cruz
Cat# sc-6493 |
immunogen = peptide mapping at the N-terminus of human cathepsin B |
polyclonal goat (liquid, PBS, gelatin, azide, affinity purified; sc-6493P blocking peptide) |
IF, IP, WB |
cathepsin B |
human, rat, mouse |
Antigen (Clone) IgG |
Distributor (Producer) |
Immunogen (Epitope) |
Host (Formulation) |
Methods glossary |
Specificity |
Reactivity top |
Cathepsin B (poly) IgG
|
Santa Cruz
Cat# sc-6491 |
immunogen = peptide mapping at the N-terminus of human cathepsin B |
polyclonal goat (liquid, PBS, gelatin, azide, affinity purified; sc-6491P blocking peptide) |
IF, WB |
cathepsin B |
human |
Cathepsin B (poly) IgG
|
Santa Cruz
Cat# sc-13985 |
immunogen = amino acids 1-339 representing full length human cathepsin B |
polyclonal rabbit (liquid, PBS, gelatin, azide) |
IF, IP, WB |
Cathepsin B |
human, rat, mouse |
Cathepsin B (CB131) IgG2b
|
AbD Serotec
Cat# MCA2373 |
immunogen = recombinant human cathepsin B |
monoclonal mouse (lyophilized, supernatant, azide) |
IH(P); not IH(F) and WB |
cathepsin B |
human |
Cathepsin B (poly) IgG
|
AbD Serotec
Cat# AHP18H |
immunogen = human cathepsin B purified from liver
|
polyclonal sheep (liquid, glycine buffered saline, EACA, benzamidine, EDTA, purified) |
ID, WB |
cathepsin B |
human |
Cathepsin B (poly)
|
AbD Serotec
Cat# 1910-8204 |
immunogen = native, from placenta
|
polyclonal sheep (liquid, PBS, azide, conj. FITC) |
IF |
cathepsin B |
human |
Cathepsin B (poly)
|
AbD Serotec
Cat# 1910-8004 |
immunogen = native, from placenta
|
polyclonal sheep (liquid, PBS, azide, purified) |
ELISA |
cathepsin B |
human (expect broad reaction) |
Cathepsin B (poly)
|
AbD Serotec
Cat# 1910-8104 |
immunogen = native, highly pure from placenta
|
polyclonal sheep (liquid, PBS, azide, glycerol, ZnCl2, MgCl2) |
ELISA |
cathepsin B |
human |
Cathepsin B (kit)
|
AbD Serotec
Cat# ICT938 |
|
|
FM; fluorescent assay |
cathepsin B |
|
Cathepsin B (kit)
|
AbD Serotec
Cat# ICT937 |
|
|
FM; fluorescent assay |
cathepsin B |
|
Cathepsin B (6A221) IgG2b
|
US Biological
Cat# C2097-11 |
immunogen = recombinant human cathepsin B |
monoclonal mouse (lyophilized, PBS, azide, supernatant) |
IH(P); not IH(F) or WB |
cathepsin B |
human |
Antigen (Clone) IgG |
Distributor (Producer) |
Immunogen (Epitope) |
Host (Formulation) |
Methods glossary |
Specificity |
Reactivity top |
Cathepsin B (6D231) IgG2b
|
US Biological
Cat# C2097-11M |
immunogen = recombinant human procathepsin B |
monoclonal mouse (liquid, PBS, thimerosal, affinity purified) |
IH, WB |
cathepsin B epitope E P G Y S P (aa 212-217); recognizes mature single or two chain cathepsin B and procathepsin B. Does not crossreact with mature cathepsins H, K, L, S, V, W or procathepsins L, S, W. |
human |
Cathepsin B (poly) IgG
|
US Biological
Cat# C2097-10 |
immunogen = human liver cathepsin B |
polyclonal rabbit (liquid, azide, purified) |
ELISA, WB |
Cathepsin B; no cross-reaction with Cathepsin L, Cathespin D or Cathepsin H. |
human |
Cathepsin B (poly) IgG
|
US Biological
Cat# C2097-03 |
immunogen = mature form of purified human liver cathepsin B |
polyclonal rabbit (liquid, thimerosal, gelatin, affinity purified) |
IH, WB |
Cathepsin B (pro and mature forms) |
human |
Cathepsin B (poly) IgG
|
US Biological
Cat# C2097-03D |
immunogen = purified human liver cathepsin B |
polyclonal sheep (liquid, GBS, aszide, EACA, Benzamidine, EDTA, purified) |
ID, WB |
cathepsin B |
human |
Cathepsin B (poly) IgG
|
US Biological
Cat# C2097-03A |
|
polyclonal rabbit (liquid, BSA, azide, affinity purified) |
IH(F, P) |
Cathepsin B (precursor and mature forms) |
human |
Cathepsin B (poly) IgG
|
US Biological
Cat# C2097-05 |
immunogen = recombinant rat pro-Cathepsin B1 |
polyclonal rabbit (liquid, tris-glycine saline, azide, protein A purified) |
IH, WB |
recognizes procathepsin B (~40kDa) and mature cathepsin B (25kD, 26kD and 30kD) |
rat, mouse |
Cathepsin D (poly)
|
BioGenex
Cat# AR205-5R Reference: Funk, 2010 |
immunogen = purified human placental Cathespin D |
polyclonal rabbit (liquid, pooled filtered antisera,
diluted in PBS, pH 7.6, BSA, sodium azide, ready to use. Also available concentrated, PU205-UP) |
IH(P) |
stains Cathepsin D |
human |
Cathepsin D (49) IgG2a
|
BD Transduction
Cat# 610800/01 |
immunogen = aa. 173-359 of human cathepsin B |
monoclonal mouse (liquid, aqueous buffered solution, purified, BSA, glycerol, azide) |
WB |
detects cathepsin D |
human |
Cathepsin L (204101) IgG2A
|
R&D Systems
Cat# MAB9521 Reference: Blum, 2007 |
immunogen = NS0-derived, recomb. human cathepsin L |
monoclonal rat (lyophilized, PBS, trehalose, protein G purified) |
ELISA, IH, IP, WB |
cathepsin L |
human, mouse |
Cathepsin L (poly) IgG
|
R&D Systems
Cat# AF1515 |
immunogen = NS0-derived, recomb. mouse cathepsin L (aa 18-334) |
polyclonal goat (lyophilized, PBS, trehalose, affinity purified, filtered) |
ELISA, IH, WB |
cathepsin L |
mouse |