|
Antibodies to CD11b, Integrin proteins
|
| From the Manufacturers
|
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
α3β1 Integrin
(M-KID2)
IgG1
|
Chemicon and Upstate are Millipore
Cat# MAB1992
Reference:
Hoe, 2009 |
immunogen = human renal carcinoma cell line (KJ29) |
monoclonal mouse
(liquid, protein-A purified IgG, PBS, BSA, azide) |
ELISA, IC, IH(P), IP, RIA |
Recognizes an epitope related to the VLA-3 complex (alpha-3/beta-1 integrin) |
human |
α5β1 Integrin
(BMA5)
IgG2b,κ
|
Chemicon and Upstate are Millipore
Cat# MAB1984 |
|
monoclonal rat
(liquid, ascites, no preservatives) |
FC, IP, IS; not WB |
detects Integrin α5/β1 |
mouse |
α5β1 Integrin
(BMB5)
IgG3
|
Chemicon and Upstate are Millipore
Cat# MAB2514 |
immunogen = mouse embryonal carcinoma P19 cells with myeloma P3X63Ag9.653 cells |
monoclonal rat
(liquid, ascites, no preservatives) |
FC, IH, IP, IS, WB |
detects Integrin α5/β1 |
mouse |
α5β1 Integrin
(BMC5)
IgG2c
|
Chemicon and Upstate are Millipore
Cat# MAB2575 |
immunogen = mouse integrin VLA-5 (alpha5beta1) from mouse embryonal carcinoma P19 cells |
monoclonal rat
(liquid, ascites) |
FC, IH(F), IS; not IH(P) |
detects Integrin α5/β1 |
mouse |
α5β1 Integrin
(HA5)
IgG2b,κ
|
Chemicon and Upstate are Millipore
Cat# MAB1999 |
|
monoclonal rat
(liquid, protein-A purified) |
FC, IP; not WB |
detects Integrin α5/β1 |
human, pig |
α5β1 Integrin
(JBS5)
IgG1
|
Chemicon and Upstate are Millipore
Cat# MAB1969 |
immunogen = Jurkat cells |
monoclonal mouse
(liquid, ascites, unpurified, no preservatives) |
IH, IP, IS |
recognizes Integrin α5/β1 |
monkey, human |
αM/CD11b Integrin
(238446)
IgG2B
|
R&D Systems
Cat# MAB16991 |
immunogen = NS0-derived rhIntegrin αM extracellular domain |
monoclonal mouse
(filtered, protein-G purified IgG fraction, PBS, trehalose, lyophilized) |
FC, IC |
detects Integrin αM |
human |
αM/CD11b Integrin
(238446)
IgG2B
|
R&D Systems
Cat# FAB16991A |
immunogen = NS0-derived rhIntegrin αM extracellular domain |
monoclonal mouse
(liquid, ready to use, APC conj.) |
FC |
detects Integrin αM |
human |
αM/CD11b Integrin
(238446)
IgG1
|
R&D Systems
Cat# MAB16992 |
immunogen = NS0-derived rhIntegrin αM extracellular domain |
monoclonal mouse
(filtered, protein-G purified IgG fraction, PBS, trehalose, lyophilized) |
ELISA, WB |
detects Integrin αM |
human |
αM/CD11b Integrin
(238446)
IgG2B
|
R&D Systems
Cat# FAB16991P |
immunogen = NS0-derived rhIntegrin αM extracellular domain |
monoclonal mouse
(liquid, ready to use, Phycoerythrin conj.) |
FC |
detects Integrin αM |
human |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
αM/CD11b Integrin
(ICRF44)
IgG1
|
R&D Systems
Cat# MAB1699 |
immunogen = mouse B cells |
monoclonal mouse
(filtered, protein-G purified, PBS, trehalose, lyophilized) |
FC, IH |
detects Integrin αM |
human |
αM/CD11b Integrin
(ICRF44)
IgG1
|
R&D Systems
Cat# BAM1699 |
immunogen = mouse B cells |
monoclonal mouse
(filtered, protein-G purified, biotinylated, PBS, trehalose, lyophilized) |
FC, IH |
detects Integrin αM |
human |
αM/CD11b Integrin
(M1/70)
IgG2B
|
R&D Systems
Cat# MAB1124 |
immunogen = Con A-activated splenocytes |
monoclonal rat
(filtered, protein-G purified IgG fraction, PBS, trehalose, lyophilized) |
FC, IH, IP |
recognizes Integrin αM |
mouse
(some cross with human reported) |
αM/CD11b Integrin
(M1/70)
IgG2B
|
R&D Systems
Cat# FAB1124F |
immunogen = Con A-activated splenocytes |
monoclonal rat
(liquid, PBS, Carboxy fluorescein conj., azide) |
FC |
recognizes Integrin αM |
mouse
(some cross with human reported) |
αM/CD11b Integrin
(M1/70)
IgG2B
|
R&D Systems
Cat# BAM1124 |
immunogen = Con A-activated splenocytes |
monoclonal rat
(filtered, protein-G purified IgG fraction, PBS, BSA, biotinylated, lyophilized) |
FC, IH, IP |
recognizes Integrin αM |
mouse
(some cross with human reported) |
αM/CD11b Integrin
(M1/70)
IgG2B
|
R&D Systems
Cat# FAB1124P |
immunogen = Con A-activated splenocytes |
monoclonal rat
(liquid, PBS, Phycoerythrin conj., azide) |
FC |
recognizes Integrin αM |
mouse
(some cross with human reported) |
αM/CD11b Integrin
(poly)
IgG
|
R&D Systems
Cat# AF1699 |
immunogen = NS0-derived rhIntegrin αM extracellular domain |
polyclonal goat
(filtered, affinity purified, PBS, trehalose, lyophilized) |
ELISA, WB |
detects Integrin αM |
human |
CD11b αM Integrin
(44)
IgG1
|
Chemicon and Upstate are Millipore
Cat# CBL145 |
immunogn = synovial cells and human monocytes |
monoclonal mouse
(liquid, purified, PBS, BSA, azide.
Also available Phycoerythrin conjugated, CBL145P) |
FC, IH, IP, IS |
reacts with the integrin heterodimer of 165 kDa which comprises the CD11b receptor |
monkey, human, baboon |
CD11b αM Integrin
(ICO-GM1)
IgG2a
|
Chemicon and Upstate are Millipore
Cat# MAB2117
Reference:
( Bhaskar K, 2010, mouse anti-CD11b, but see also CBL145) |
immunogn = leukemic cells of CML |
monoclonal mouse
(liquid, purified, PBS, azide) |
FC, IC, IH(P), IP, IS |
specific to CD11b; reacts with the CR3-receptor of inactivated C3b component of complement (C3bi) |
human |
CD11b αM Integrin
(M1/170.15.1)
IgG2b
|
Chemicon and Upstate are Millipore
Cat# CBL1313 |
immunogn = B10 mouse spleen cells enriched with T cells |
monoclonal rat
(liquid, purified, PBS, azide
Also available Phycoerythrin conjugated, CBL1313P) |
FC, IF, IH(F), IP; not IH(P) |
specific to CD11b; recognizes a differentiation antigen situated on mononuclear phagocytes and granulocytes; the expression of this antigen (Mac 1) increases during monocyte maturation |
mouse |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
CD11b αM Integrin
(M1/170.15.1)
IgG2b
|
Chemicon and Upstate are Millipore
Cat# MAB1387Z |
immunogn = T cell enriched splenocytes from B10 mice |
monoclonal rat
(liquid, protein-G purified, PBS, no preservatives) |
FC, IF, IH(F), IP, IS; not IH(P) |
recognizes the murine alphaM integrin chain; stains polymorphonuclear cells, monocytes and NK cells |
human, mouse |
CD11b αM Integrin
(OX-42)
IgG2a
|
Chemicon and Upstate are Millipore
Cat# CBL1512 |
immunogn = rat peritoneal macrophages |
monoclonal mouse
(liquid, protein-A affinity purified, PBS, azide.
Also available protein-G purified, PBS, no preservatives, CBL1512Z, or FITC conjugated, CBL1512F) |
FC, IH(P), IP |
recognizes most macrophages (including resident peritoneal and activated macrophages), Kupffer cells, but only about 35% of alveolar macrophages; also labels dendritic cells extensively, granulocytes and cells with the morphology of microglia in brain |
rat |
CD11b /αM Integrin
(17aba)
IgM,κ
|
ATCC
Cat# CRL-248™ |
immunogen = human acute monocytic leukemia cells (FAM M6) |
monoclonal mouse
(frozen liquid, Modified Dulbecco's medium, heat-inactivated gamma globulin free horse serum) |
|
reacts with human CD11b, and blocks all known CD11b dependent activities in vitro |
human, rat, dog |
CD11b /αM Integrin
(44aacb)
IgG2a,κ
|
ATCC
Cat# CRL-249™ |
immunogen = human acute monocytic leukemia cells (FAM M6) |
monoclonal mouse
(frozen liquid, Modified Dulbecco's medium, heat-inactivated gamma globulin free horse serum) |
|
reacts with human CD11b, and blocks a variety of CD11b dependent activities in vitro |
human, primate (various) |
CD11b /αM Integrin
(LM2/1.6.11)
IgG1
|
ATCC
Cat# CRL-204™ |
immunogen = immunoprecipitates of human granulocyte lysates |
monoclonal mouse
(frozen liquid, ATCC complete growth medium) |
|
reacts with CD11b/MAC-1, and is specific for the alpha chain |
human |
CD11b /αM Integrin
(OKM-1)
IgG2b
|
ATCC
Cat# CRL-8026™ |
immunogen = human peripheral blood mononuclear cells (monocytes) |
monoclonal mouse
(frozen liquid, ATCC-formulated Dulbecco's Modified Medium, fetal bovine serum) |
|
reacts with CD11b |
human |
CD11b /integrin α M-chain
(5C6)
IgG2b
|
AbD Serotec
Cat# MCA711
References:
Heneka, 2010
Lee, 2007 |
immunogen = Thioglycollate elicted Peritoneal Macrophages (TPM) |
monoclonal rat
(liquid, purified IgG, PBS, azide
See list of other formats) |
FC, IH(F), IP |
recognizes mouse complement type 3 receptor (CR3) and precipitates a heterodimer of 165 and 95kD |
human, mouse |
CD11b /integrin α M-chain
(ED7)
IgG1
|
AbD Serotec
Cat# MCA618R |
immunogen = Rat spleen cell homogenate |
monoclonal mouse
(liquid, affinity purified IgG, azide) |
FC, IH(F); not IH(P) |
reacts with rat spleen cell homogenate; recognises small ramified microglia in the central nervous system |
rat |
CD11b /integrin α M-chain
(M1/70.15)
IgG2b
|
AbD Serotec
Cat# MCA74 |
immunogen = T cell enriched splenocytes from B10 mice |
monoclonal rat
(liquid, supernatant, Tris saline, calf serum, azide
See list of other formats) |
FC, IH(F, P), IP |
recognizes the murine CD11b cell surface antigen |
human, mouse, rabbit |
CD11b /integrin α M-chain
(M1/70.15)
IgG2b and IgG1
|
AbD Serotec
Cat# DC055
Reference:
Grathwohl, 2009 |
immunogen = T cell enriched splenocytes from B10 mice |
monoclonal rat
(PBS, BSA, azide, lyophilized
dual color, ALEXA FLUOR® 488 / RPE) |
FC, IH |
recognizes murine CD115 and CD11b cell surface antigen |
mouse |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
CD11b /Mac-1α
(M1/70)
IgG2b,κ
|
eBioscience
Cat# 15-0112
Reference:
Chambers, 2007 |
|
monoclonal rat
(liquid, phosphate buffer, azide, PE-Cy5 conj.) |
FC |
specific for CD11b and Mac-1α /αM β2 integrin |
mouse |
CD11b /Mac-1α
(ICRF44/44)
IgG1,κ
|
BD Pharmingen
Cat# 558123 |
immunogen = Human monocytes |
monoclonal mouse
(liquid, affinity purified, aqueous buffered solution, azide, Pacific Blue™ conj.
Also available APC-Cy™7 conj, #557754
PE-Cy™5 conj, #555389
PE-Cy™7 conj, #557743) |
FC |
reacts with CD11b; significantly inhibits polymorphonuclear leukocyte aggregation in response to fMLP. |
human |
CD11b /Mac-1α
(ICRF44/44)
IgG1,κ
|
BD Pharmingen
Cat# 555388 |
immunogen = Human monocytes |
monoclonal mouse
(liquid, affinity purified, aqueous buffered solution, BSA, azide, R-PE conj.
Also available Biotin Conj, #555387) |
FC |
reacts with CD11b; significantly inhibits polymorphonuclear leukocyte aggregation in response to fMLP. |
human |
CD11b /Mac-1α
(ICRF44/44)
IgG1,κ
|
BD Pharmingen
Cat# 557701 |
immunogen = Human monocytes |
monoclonal mouse
(aqueous buffered solution, affinity purified, stabilizer, azide, Alexa Fluor® 488 conj.
Also available Alexa Fluor® 700 conj, #557918) |
FC |
reacts with CD11b; significantly inhibits polymorphonuclear leukocyte aggregation in response to fMLP |
human |
CD11b /Mac-1α
(ICRF44/44)
IgG1,κ
|
BD Pharmingen
Cat# 550374 |
immunogen = Human monocytes |
monoclonal mouse
(aqueous buffered solution, affinity purified, BSA, goat serum, azide) |
IH(F); WB reported. Not IH(P) |
detects CD11b; significantly inhibits polymorphonuclear leukocyte aggregation in response to fMLP. |
human |
CD11b /Mac-1α
(ICRF44/44)
IgG1,κ
|
BD Pharmingen
Cat# 555386 |
immunogen = Human monocytes |
monoclonal mouse
(liquid, affinity purified, aqueous buffered solution, azide.
Also available No azide/low endotoxin, #555385) |
FC, IH(F); WB reported |
reacts with CD11b; significantly inhibits polymorphonuclear leukocyte aggregation in response to fMLP. |
human |
CD11b /Mac-1α
(M1/70)
IgG2b,κ
|
BD Pharmingen
Cat# 557960 |
immunogen = C57BL/10 mouse splenic T cells and concanavalin A-activated C57BL/10 splenocytes |
monoclonal rat
(aqueous buffered solution, affinity purified, stabilizer, azide, Alexa Fluor® 700 conj.) |
FC |
reacts with CD11b; reportedly blocks cell adherence
and C3bi binding, but it does not block cell-mediated lysis. Cross-reaction of mAb M1/70 with CD11b on human monocytes,
polymorphonuclear leukocytes, and NK cells has been reported |
human, mouse |
CD11b /Mac-1α
(M1/70)
IgG2b,κ
|
BD Pharmingen
Cat# 557657 |
immunogen = C57BL/10 mouse splenic T cells and concanavalin A-activated C57BL/10 splenocytes |
monoclonal rat
(aqueous buffered solution, affinity purified, stabilizer, azide, APC-Cy7 conj.
Also available PerCP-Cy5.5 conj, #550993
PE-Cy™7 conj, #552850
APC conj, #553312
PE conj, #553311) |
FC |
reacts with CD11b; reportedly blocks cell adherence
and C3bi binding, but it does not block cell-mediated lysis. Cross-reaction of mAb M1/70 with CD11b on human monocytes,
polymorphonuclear leukocytes, and NK cells has been reported |
human, mouse |
CD11b /Mac-1α
(M1/70)
IgG2b,κ
|
BD Pharmingen
Cat# 557686 |
immunogen = C57BL/10 mouse splenic T cells and concanavalin A-activated C57BL/10 splenocytes |
monoclonal rat
(aqueous buffered solution, affinity purified, stabilizer, azide, Alexa Fluor® 647 conj.) |
FC |
reacts with CD11b; reportedly blocks cell adherence
and C3bi binding, but it does not block cell-mediated lysis. Cross-reaction of mAb M1/70 with CD11b on human monocytes,
polymorphonuclear leukocytes, and NK cells has been reported |
human, mouse |
CD11b /Mac-1α
(M1/70)
IgG2b,κ
|
BD Pharmingen
Cat# 557672 |
immunogen = C57BL/10 mouse splenic T cells and concanavalin A-activated C57BL/10 splenocytes |
monoclonal rat
(aqueous buffered solution, affinity purified, stabilizer, azide, Alexa Fluor® 488 conj.) |
FC |
reacts with CD11b; reportedly blocks cell adherence
and C3bi binding, but it does not block cell-mediated lysis. Cross-reaction of mAb M1/70 with CD11b on human monocytes,
polymorphonuclear leukocytes, and NK cells has been reported |
human, mouse |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
CD11b /Mac-1α
(M1/70)
IgG2b,κ
|
BD Pharmingen
Cat# 550282
Reference:
Kim, 2009 |
immunogen = C57BL/10 mouse splenic T cells and concanavalin A-activated C57BL/10 splenocytes |
monoclonal rat
(affinity purified, IHC diluent containing serum and azide) |
FC, IH(F); Blocking and IP reported. Not IH(P) |
detects CD11b; stains the macrophages, granulocytes, dendritic cells, and NK cells |
human, mouse |
CD11b /Mac-1α
(M1/70)
IgG2b,κ
|
BD Pharmingen
Cat# 553309 |
immunogen = C57BL/10 mouse splenic T cells and concanavalin A-activated C57BL/10 splenocytes |
monoclonal rat
(aqueous buffered solution, affinity purified, stabilizer, azide, Biotin conj.
Also available FITC conj, #553310) |
FC; IF reported |
reacts with CD11b; reportedly blocks cell adherence
and C3bi binding, but it does not block cell-mediated lysis. Cross-reaction of mAb M1/70 with CD11b on human monocytes,
polymorphonuclear leukocytes, and NK cells has been reported |
human, mouse |
CD11b /Mac-1α
(M1/70)
IgG2b,κ
|
BD Pharmingen
Cat# 553308 |
immunogen = C57BL/10 mouse splenic T cells and concanavalin A-activated C57BL/10 splenocytes |
monoclonal rat
(aqueous buffered solution, affinity purified, azide
Also available No azide/low endotoxin, #553307, for IP, blocking) |
FC; IF reported |
reacts with CD11b; reportedly blocks cell adherence
and C3bi binding, but it does not block cell-mediated lysis. Cross-reaction of mAb M1/70 with CD11b on human monocytes,
polymorphonuclear leukocytes, and NK cells has been reported |
human, mouse |
CD11b/c
(poly)
|
Novus Biologicals
Cat# NB110-40766 |
immunogen =
synthetic peptide made to an internal region (within residues 500-600) of the mouse CD11 (b/c) protein |
polyclonal rabbit
(liquid, antisera, Tris-glycine, saline, azide, affinity purified.
Also available as HRP, Biotin or HiLyte Fluor™ 488 conj.) |
IH(P), WB |
reacts with CD11; doublet is seen ~95 and 103 kDa in WB |
human, mouse
(others not tested) |
CD11b/CD18 αMβ2 Integrin
(ED7)
IgG1
|
Chemicon and Upstate are Millipore
Cat# MAB1408 |
immunogn = spleen cell homogenate with FCA |
monoclonal mouse
(liquid, ascites, protein-A affinity purified, PBS, azide.) |
IC, IH |
recognizes a membrane antigen on rat macrophages, monocytes, dendritic cells and granulocytes; recognizes small ramified (micro) glial cells in the central nervous system |
rat |
CR3/CD11b
(OX42)
IgG2a
|
AbD Serotec
MCA275R
Reference:
Ryu, 2009 |
immunogen = rat peritoneal macrophages |
monoclonal goat
(liquid, affinity purified, PBS, azide
See list of conjugates) |
FC, IH(F), IP |
reacts with the rat equivalent of human CD11b, the receptor for the iC3b component of complement; CD11b is expressed on most macrophages |
rat
(expect others) |
Integrin-β-5
(poly)
|
AnaSpec
Cat# 53588 |
immunogen = a synthetic peptide from the C-terminus of human integrin 5 subunit |
polyclonal rabbit
(liquid, affinity purified, PBS, BSA, azide) |
IF, IH(P), IP, WB |
detects Integrin-β5 |
human, rat, mouse, hamster |
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