|
Antibodies to CaM Kinase II proteins
|
| From the Manufacturers
|
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
α-CaM Kinase II
(CBα-2)
IgG2a,κ
|
Histo-Line
Cat# 13-7300
supplied by Zymed |
Immunogen = purified rat brain CaM Kinase |
monoclonal mouse
(liquid, PBS, azide, affinity purified) |
ELISA, IH(P), IP, WB |
Specific for α subunit of CaM Kinase II |
human, rat, mouse, goldfish |
α-CaM Kinase II
(CBα-2)
IgG2a,κ
|
Zymed/ Invitrogen
Cat# 137300 |
Immunogen = purified rat brain CaM Kinase |
monoclonal mouse
(liquid, PBS, azide, affinity purified) |
ELISA, IH(P), IP, WB |
Specific for α subunit of CaM Kinase II |
human, rat, mouse, fish (various) |
β-CaM Kinase II
(CBβ-1)
IgG2b,κ
|
Histo-Line
Cat# 13-9800
supplied by Zymed |
Immunogen = purified rat brain CaM Kinase |
monoclonal mouse
(liquid, PBS, azide, Protein A purified) |
ELISA, IH(P), IP, WB |
Specific for β subunit of CaM Kinase II |
human, rat, mouse, sea urchin, goldfish |
β-CaM Kinase II
(CBβ-1)
IgG2b,κ
|
Zymed/ Invitrogen
Cat# 13-9800 |
Immunogen = purified rat brain CaM Kinase |
monoclonal mouse
(liquid, PBS, azide, Protein A purified) |
ELISA, IH(P), IP, WB |
Specific for β subunit of CaM Kinase II |
human, rat, mouse, sea urchin, fish (various) |
CaM KII phos Thr305
(poly)
Ig
|
Sigma Aldrich
Cat# P5997 |
Immunogen = synthetic peptide corresp. to aa residues surrounding the rat CaMKinase II phosphorylated on threonine 305 |
polyclonal rabbit
(liquid, whole serum, HEPES, NaCl, BSA, glycerol) |
DB, WB |
CaM Kinase IIα (50 kDa) and β (60 kDa) phosphorylated at threonine 305 |
human, rat, mouse, bovine, xenopus, chicken |
CaM KIIα
(poly)
IgG
|
Sigma Aldrich
Cat# C6974 |
Immunogen = synthetic peptide (K W Q I V H F H R S G A P S V L P H) corresp. to the C-terminal aa (461-478) region of rat CaMKinase IIα |
polyclonal rabbit
(liquid, PBS, azide) |
IP, WB |
CaMKinase IIα (50 kDa); no cross-reaction with CaMKIIβ, γ and δ isoforms (60 kDa) |
human, rat, mouse, chicken |
Cam Kinase II
(6G9)
IgG1
|
A B R - Thermo Scientific
Cat# MA1-048 |
immunogen = partially purified rat CaM Kinase II |
monoclonal mouse
(liquid, PBS, azide) |
IF, IP, WB |
CaMK2α (50kDa, phospho and non-phospho); no cross with beta subunit |
rat |
Cam Kinase II
(poly)
IgG
|
A B R - Thermo Scientific
Cat# PA1-14077 |
immunogen = synthetic non-phospho peptide corresp. to human CaM Kinase II around the phosphorylation site of threonine 286 (Q-E-T-P-V-D) |
polyclonal rabbit
(liquid, PBS, NaCl, azide, glycerol) |
WB |
detects CaMKII |
human |
Cam Kinase II
(poly)
IgG
|
A B R - Thermo Scientific
Cat# PA1-21407 |
immunogen = synthetic peptide corresp. to the N-terminal residues C7 T R F T D E Y Q L F E E L20 of rat CaMKII (delta isoform), conj. to KLH |
polyclonal rabbit
(liquid, BBS, affinity purified) |
WB |
detects CaMKII |
human, rat, guinea pig, mouse, hamster, rabbit, bovine, chicken |
CaM Kinase II
(poly)
IgG
|
Calbiochem EMD Biosciences
Cat# 208703 |
Immunogen = synthetic peptide corresp. to 14 aa (7-20, N-terminal), C T R F T D E Y Q L F E E L, based on the rat CaM Kinase II delta isoform, conj. to KLH |
polyclonal rabbit
(liquid, Tris-glycine, azide, affinity purified) |
ELISA, WB |
CaM Kinase II all three isoforms |
rat, mouse |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
CaM Kinase II
(6G9)
IgG1
|
Chemicon and Upstate are Millipore
Cat# MAB8699
Reference:
Townsend, 2007 |
Immunogen = purified type II CaM Kinase |
monoclonal mouse
(liquid, potassium phosphate saline, Protein A purified) |
IC, IF, IH, RIA, WB |
recognizes both the recombinant and native phosphorylated and non-phosphorylated α subunit of CaM kinase II only; no cross-reaction with the 60kDa β subunit in either phosphorylation state |
human, rat, mouse, bovine, frog, chicken |
CaM Kinase II
(6G9)
IgG1
|
Chemicon and Upstate are Millipore
Cat# MAB3119 |
Immunogen = partially purified rat CaMK II |
monoclonal mouse
(liquid, protein-G purified, PBS, glycerol, azide) |
IC, IF, IH, RIA, WB |
CaM Kinase II, α isoform, both phos. and non-phos. |
human, rat, mouse, bovine, frog, chicken
(other species not tested) |
CaM Kinase II
(poly)
Ig
|
Chemicon and Upstate are Millipore
Cat# AB3111
discontinued
suggested replacement |
Immunogen = rat CaMK II synthetic peptide (a.a. 7-20), conjugated to KLH |
polyclonal rabbit
(liquid, purified Ig, BBS) |
WB; not IP |
CaM Kinase II, all isoforms but β |
human, rat, guinea pig, mouse, hamster, rabbit, bovine, chicken
(other species not tested) |
Cam Kinase II
(6G9)
IgG1
|
Affiniti - Biomol
Cat# SA162
produced by Enzo Life Sciences |
Immunogen = partially purified rat CaMK2 |
monoclonal mouse
(liquid, ascites, purified, PBS, azide) |
IH, WB |
CaMK2α (50kDa, α-subunit, phosphorylated and non-phosphorylated) |
rat, mouse, frog, chicken |
CaM Kinase II
(kit)
|
MBL International
Cat# CY-1173 |
|
mouse
(single site, non radioisotopic, phospho specific) |
ELISA |
phospho CaMKII |
|
CaM Kinase II
(poly)
|
RDI /Fitzgerald
Cat# 20R-CR009
(was RDI-CAMK2abR) |
Immunogen = synthetic peptide aa 7-20 (CTRFTDEYQLFEEL) from rat CaM kinase II, conj. to KLH |
polyclonal rabbit
(liquid, sodium borate, boric acid, saline, EDAT) |
WB |
60 kDa CaMKII; expect α, δ and γ, but not β |
human, rat, guinea pig, mouse, hamster, rabbit, bovine, chicken |
CaM Kinase II
(poly)
|
Rockland
Cat# 600-401-873 |
Immunogen = synthetic peptide corresp. to aa 6-23 of human CaMKinase II protein |
polyclonal rabbit
(liquid, potassium phosphae saline, azide, affinity purified) |
ELISA, IF, WB |
CaMKinaseII |
human, rat, mouse, rabbit, pig, zebra, dog, fish (various), frog, chicken |
CaM Kinase II
(poly)
IgG
|
Upstate and Chemicon are Millipore
Cat# 07-1496 |
immunogen = synthetic peptide corresp. to the N-terminus of Rat CaMK II |
polyclonal rabbit
(liquid, affinity purified IgG, BBS, PBS, azide) |
WB |
detects CaM Kinase II isoforms, 50-72 kDa; reacts with all but the β isoform |
human, rat, guinea pig, mouse, hamster, rabbit, bovine, chicken |
CaM Kinase II
(kit)
|
Upstate and Chemicon are Millipore
Cat# 17-135 |
|
(radioactive detection;
capacity, 200 assays) |
Kinase assay |
CaM Kinase II |
|
CaM Kinase II
(kit)
|
Upstate and Chemicon are Millipore
Cat# 32-089 |
|
(FP-645nm FarRed Assay) |
Kinase assay |
CaM Kinase II |
|
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
CaM Kinase II
(kit)
|
Upstate and Chemicon are Millipore
Cat# 32-009 |
|
(FP Fluorescein Green Assay) |
Kinase assay |
CaM Kinase II |
|
CaM Kinase II
(6G9)
IgG1
|
VWR International, Inc.
Cat# 100177-616
supplied by Chemicon |
Immunogen = partially purified rat CaMK II |
monoclonal mouse
(liquid, protein-G purified, PBS, PMSF, glycerol) |
IC, IF, IH, RIA, WB |
CaM Kinase II, alpha isoform, both phos. and non-phos. |
human, rat, mouse, bovine, frog, chicken |
CaM Kinase II
(6G9)
IgG1
|
VWR International, Inc.
Cat# 100179-230
supplied by Millipore |
Immunogen = purified type II CaM Kinase |
monoclonal mouse
(liquid, potassium phosphate saline, Protein A purified) |
IC, IF, IH, RIA, WB |
recognizes both the recombinant and native phosphorylated and non-phosphorylated α subunit of CaM kinase II only; no cross-reaction with the 60kDa β subunit in either phosphorylation state |
human, rat, mouse, bovine, frog, chicken |
CaM Kinase II
(poly)
|
VWR International, Inc.
Cat# RL600-401-873
supplied by Rockland |
Immunogen = synthetic peptide corresp. to aa 6-23 of human CaMKinase II protein |
polyclonal rabbit
(liquid, potassium phosphae saline, azide, affinity purified) |
ELISA, IF, WB |
CaMKinaseII |
human, rat, mouse, rabbit, pig, zebra, dog, fish (various), frog, chicken |
CaM Kinase II
(kit)
|
VWR International, Inc.
Cat# 100191-740
supplied by MBL |
|
mouse
(single site, non radioisotopic, phospho specific) |
ELISA |
phospho CaMKII |
|
Cam Kinase II α phos Thr286
(poly)
IgG
|
A B R - Thermo Scientific
Cat# PA1-26727 |
immunogen = synthetic phosphopeptide corresp. to a portion of human CaM Kinase II α containing threonine 286
|
polyclonal rabbit
(liquid, PBS, BSA, NaCl, azide, glycerol, affinity purified) |
WB |
detects CaMKII α (phospho T286) |
human
(expect mouse, rat) |
CaM Kinase II β
(poly)
|
ImmunoKontact
Cat# 221-MG-1C11B |
|
polyclonal
|
|
|
human |
Cam Kinase II phos Thr286
(22B1)
IgG1
|
A B R - Thermo Scientific
Cat# MA1-047 |
Immunogen = synthetice peptide M281 H R Q E(pT) V D C L K K F N294 of rat CaM KII |
monoclonal mouse
(liquid, PBS, azide, purified) |
ELISA, IC, IF, IH, IP, WB |
CaM KinaseIIα phosT286; no cross with non-phos or with beta subunit either phos or non-phos |
rat, mouse |
Cam Kinase II phos Thr286
(poly)
IgG
|
A B R - Thermo Scientific
Cat# PA1-4614 |
Immunogen = phosphopeptide corresp. to aa residues surrounding the phospho-Thr286 found in rat brain CaM Kinase II |
polyclonal rabbit
(liquid, HEPES, NaCl, BSA, glycerol, affinity purified) |
WB |
specific for the ~50 kDa α-CaM Kinase II subunit and the ~60 kDa β-CaM Kinase II subunit phosphorylated at Thr286 |
human, rat, mouse, xenopus
(expect bovine, chicken) |
Cam Kinase II phos Thr286
(poly)
IgG
|
A B R - Thermo Scientific
Cat# PA1-12734 |
immunogen = synthetic phosphopeptide corresp. to rat brain CaM Kinase II |
polyclonal rabbit
(10 miniblots in HEPES, NaCl, BSA, glycerol) |
WB |
detects phospho-CAM Kinase II |
human, rat, mouse, xenopus |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
Cam Kinase II phos Thr286
(poly)
IgG
|
A B R - Thermo Scientific
Cat# PA1-14076 |
immunogen = synthetic phosphopeptide corresp. to human brain CaM KinaseII around the phosphorylation site of threonine 286 (Q-E-T-P-V-D)
|
polyclonal rabbit
(liquid, PBS, NaCl, azide, glycerol) |
WB |
detects phospho-CAM Kinase II |
human, mouse |
Cam Kinase II phos Thr286
(poly)
IgG
|
A B R - Thermo Scientific
Cat# PA1-22993 |
immunogen = synthetic phosphopeptide derived from human CaMKII around the phosphorylation site of threonine 286 (Q-E-TP-V-D) |
polyclonal rabbit
(liquid, PBS, glycerol, saline, azide, affinity purified) |
WB |
detects CaMKII (phospho T286) |
human, rat, mouse |
Cam Kinase II phos Thr286
(poly)
|
A B R - Thermo Scientific
Cat# PA1-84819 |
Immunogen = synthetice peptide corresp. to an amino acid sequence within CAMKII which includes phosphor. Thr286 |
polyclonal rabbit
(liquid, HEPES, BSA, glycerol, azide) |
WB |
detects CaM KinaseII phosThr286 |
rat
(expect human, mouse, Xenopus) |
Cam Kinase II phos Thr286
(22B1)
IgG1
|
Affiniti - Biomol
Cat# SA-234 |
Immunogen = synthetice peptide M (281) H R Q E(pT) V D C L K K F N (294) of CMK2 phosThr286 |
monoclonal mouse
(liquid, PBS, azide) |
IF, WB |
CaM KinaseIIα phosT286; no cross with non-phos protein |
rat, mouse |
Cam Kinase II phos Thr286
(poly)
IgG1
|
Affiniti - Biomol
Cat# SA-523 |
Immunogen = phosphopeptide corresp. to aa residues surrounding Thr286 of rat brain CaMKinase II |
polyclonal mouse
(liquid, HEPES, NaCl, BSA, glycerol, affinity purified) |
WB |
CaM KinaseIIα (50kDa) and CaM KinaseIIβ (60kDa) when phosphorylated on T286 |
human, rat, mouse, xenopus |
CaM Kinase II phos Thr286
(poly)
|
Histo-Line
Cat# 36-8700
supplied by Zymed |
Immunogen = synthetic phospho-peptide corresp. to aa surrounding the phosphorylated Thr286 of Cam Kinase II |
polyclonal rabbit
(liquid, Hepes, NaCl, BSA, glycerol) |
WB |
CaM Kinase II phosphorylated at Thr286 (50kDa, 60kDa) |
rat |
CaM Kinase II phos Thr286
(poly)
|
ImmunoKontact
Cat# 221-MG-1CK2 |
immunogen = synthetic peptide corresp. to aa residues surrounding the phosphorylated Thr286 found in rat brain CaM Kinase II |
polyclonal rabbit
(liquid, HEPES, NaCl, BSA, glycerol, purified) |
WB |
specific for ~50kDa α--CaM Kinase II and the ~60kDa β-CaM Kinase II phosphorylated at Thr286 |
human, rat |
CaM Kinase II phos Thr286
(poly)
|
Phospho- solutions
Cat# p1005-286
Reference:
Townsend, 2007 |
Immunogen = phosphopeptide corresp. to aa surrounding Thr286 from rat brain CaM Kinase II |
polyclonal rabbit
(liquid, Hepes, NaCl, BSA, glycerol, affinity purified) |
WB |
CaM Kinase II α subunit (~50kDa) and β subunit (~60kDa) phosphorylated at Thr286 |
human, rat, mouse, bovine, xenopus, chicken |
CaM Kinase II phos Thr286
(poly)
|
Zymed/ Invitrogen
Cat# 36-8700 |
Immunogen = synthetic phospho-peptide corresp. to aa surrounding the phosphorylated Thr286 of Cam Kinase II |
polyclonal rabbit
(liquid, Hepes, NaCl, BSA, glycerol) |
WB |
CaM Kinase II phosphorylated at Thr286 (50kDa, 60kDa) |
rat |
Cam Kinase II phos Thr305
(poly)
|
A B R - Thermo Scientific
Cat# PA3-4615 |
Immunogen = phosphopeptide corresp. to aa residues surrounding the phospho-Thr found in rat brain CaM Kinase II |
polyclonal rabbit
(neat serum) |
WB |
specific for the ~50 kDa α-CaM Kinase II subunit and the ~60 kDa β-CaM Kinase II subunit phosphorylated at Thr305 |
human, rat, mouse, bovine, xenopus, chicken |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
Cam Kinase II phos Thr305
(poly)
IgG
|
A B R - Thermo Scientific
Cat# PA1-12735 |
immunogen = synthetic phosphopeptide corresp. to rat brain CaM Kinase II |
polyclonal rabbit
(10 miniblots as whole antisera) |
WB |
detects phospho-CAM Kinase II |
human, rat, mouse, bovine, xenopus, chicken |
Cam Kinase II phos Thr305
(poly)
IgG1
|
Affiniti - Biomol
Cat# SA-524 |
Immunogen = phosphopeptide corresp. to aa residues surrounding Thr305 of rat brain CaMKinase II |
polyclonal mouse
(liquid, HEPES, NaCl, BSA, glycerol, affinity purified) |
WB |
CaM KinaseIIα (50kDa) and CaM KinaseIIβ (60kDa) when phosphorylated on T305 |
human, rat, mouse, xenopus |
CaM Kinase II phos Thr305
(poly)
|
Histo-Line
Cat# 36-7800
supplied by Zymed |
Immunogen = synthetic phospho-peptide corresp. to aa surrounding the phosphorylated Thr305 of CaM Kinase II |
polyclonal rabbit
(liquid, Hepes, NaCl, BSA, glycerol) |
WB |
CaM Kinase II phosphorylated at Thr305 |
rat |
CaM Kinase II phos Thr305
(poly)
IgG
|
ImmunoKontact
Cat# 223-MG-CAMK2 |
immunogen = peptide corresp. to aa surrounding the phosphorylated Thr305 found in rat brain CaM Kinase II |
polyclonal rabbit
(liquid, azide, neat serum) |
WB |
CaMKinase 11 phos Thr305 |
rat
(expect bovine, chicken, human , mouse and xenopus) |
CaM Kinase II phos Thr305
(poly)
|
Phospho- solutions
Cat# p1005-305
Reference:
Townsend, 2007 |
Immunogen = phosphopeptide corresponding to aa surrounding the phospho-Thr305 from rat brain CaM Kinase II |
polyclonal rabbit
(liquid, Neat serum) |
WB |
CaM Kinase II α subunit (~50kDa) and β subunit (~60kDa) phosphorylated at Thr305 |
human, rat, mouse, dog, bovine, zebra fish, xenopus, chicken |
CaM Kinase II phos Thr305
(poly)
|
Zymed/ Invitrogen
Cat# 36-7800 |
Immunogen = synthetic phospho-peptide corresp. to aa surrounding the phosphorylated Thr305 of CaM Kinase II |
polyclonal rabbit
(liquid, Hepes, NaCl, BSA, glycerol) |
WB |
CaM Kinase II phosphorylated at Thr305 |
rat |
CaM Kinase IIα
(6G9)
IgG1
|
Calbiochem EMD Biosciences
Cat# NB12 |
Immunogen = partially purified rat CaM kinase II |
monoclonal mouse
(liquid, PBS, azide) |
IF, WB |
CaM Kinase II α subunit (50kDa, phosphorylated and non-phosphorylated).
Does not cross-react with the β subunit (60kDa) in either phosphorylation state. |
rat |
CaM Kinase IIα
(6G9)
IgG1
|
VWR International, Inc.
Cat# 80000-924
supplied by Calbiochem |
Immunogen = partially purified rat CaM kinase II |
monoclonal mouse
(liquid, PBS, azide) |
IF, WB |
CaM Kinase II α subunit (50kDa, phosphorylated and non-phosphorylated).
Does not cross-react with the β subunit (60kDa) in either phosphorylation state. |
rat |
CaM Kinase IIα phos
(22B1)
IgG1
|
VWR International, Inc.
Cat# 80000-926
supplied by Calbiochem |
Immunogen = phosphorylated (Thr286) peptide corresponding to aa 281-294 (M H R Q E T V D C L K K F N) of the mouse/rat CaM kinase II α subunit |
monoclonal mouse
(liquid, PBS, azide, purified) |
ELISA, IF, WB |
CaM Kinase II α subunit |
rat |
CaM Kinase IIα phos Thr286
(22B1)
IgG1
|
Calbiochem EMD Biosciences
Cat# NB13 |
Immunogen = phosphorylated (Thr286) synthetic peptide corresp. to aa 281-294 (M H R Q E T V D C L K K F N) of the mouse/rat CaM kinase II α subunit |
monoclonal mouse
(liquid, PBS, azide, purified) |
ELISA, IF, WB |
recognizes the ~50 kDa α subunit of CaM kinase II phosphorylated at Thr286. Does not cross-react with un-phosphorylated a subunit of CaM kinase II or with the b subunit in either phosphorylation state |
rat |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
CaM Kinase II, α subunit
(6G9)
IgG1
|
Upstate and Chemicon are Millipore
Cat# 05-532 |
Immunogen = partially purified rat CaM Kinase II, α-subunit |
monoclonal mouse
(liquid, tris-glycine saline, glycerol, azide, Protein G purified) |
IH, WB |
CaM Kinase II α-subunit (50 kDa) |
rat, bovine |
CaM Kinase II, α subunit
(6G9)
IgG1
|
VWR International, Inc.
Cat# 101229-966
supplied by Chemicon |
Immunogen = partially purified rat CaM Kinase II, α-subunit |
monoclonal mouse
(liquid, tris-glycine saline, glycerol, azide, Protein G purified) |
IH, WB |
CaM Kinase II α-subunit (50 kDa) |
rat, bovine |
CaM Kinase II, α subunit, phospho Thr286
(22B1)
IgG1
|
VWR International, Inc.
Cat# 101229-968
supplied by Chemicon |
Immunogen = synthetic peptide corresponding to aa 281-294 of rat CaM Kinase II α-subunit |
monoclonal mouse
(liquid, tris-glycine saline, azide, Protein G purified) |
WB |
Recognized phospho-CaM Kinase II α-subunit (50 kDa). Additional proteins observed at 125 kDa and 135 dKa. |
human, rat, mouse, chicken |
CaM Kinase II, α/β, phospho Thr286/287
(poly)
IgG
|
VWR International, Inc.
Cat# 101230-272
supplied by Chemicon |
Immunogen = synthetic peptide, K M H R Q E [pT]V D C L K, correspond. to aa 281-291 of rat CaM Kinase II α-subunit |
polyclonal rabbit
(liquid, tris-glycine saline, glycerol, azide) |
WB |
Recognized phospho-CaM Kinase II α (50 kDa) and β (60 kDa) subunits. |
rat, mouse, porcine, drosophila, xenopus |
CaM Kinase II, γ subunit
(poly)
IgG
|
Upstate and Chemicon are Millipore
Cat# 07-743 |
Immunogen = 6His-tagged fusion protein corresp. to residues 7-305 of ferret CaM Kinase II, γ subunit |
polyclonal rabbit
(liquid, affinity purified, Tris, glycerol, azide) |
WB |
CaM Kinase II γ-subunit |
rat, ferret |
Cam KinaseII
(6G9)
IgG1
|
Acris Antibodies
Cat# SM5005P |
Immunogen = partially purified rat CaMK2 |
monoclonal mouse
(liquid, PBS, azide) |
IF, IP, WB |
CaMK2α (50kDa, phosphorylated and non-phosphorylated) |
rat |
Cam KinaseII phos Thr286
(22B1)
IgG1
|
Acris Antibodies
Cat# SM5004P |
Immunogen = synthetic peptide M (281) H R Q E (pT) V D C L K K F N (294) of CMK2 phosThr286 |
monoclonal mouse
(liquid, PBS, azide, purified) |
ELISA, IP, WB |
CaM KinaseIIα phosT286 |
rat |
Cam KinaseII phos Thr286
(poly)
Ig
|
Acris Antibodies
Cat# SP7030P |
|
polyclonal rabbit
(affinity purified) |
WB |
|
human, rat, mouse, xenopus |
Cam KinaseIIα
(1C6)
IgG2a
|
Acris Antibodies
Cat# H00000815-M05 |
Immunogen = partially recombinant CaMK2α with GST tag (AAH40457, aa 305-410) |
monoclonal mouse
(liquid, PBS, azide, purified) |
ELISA, WB |
CaMK2α |
human |
Cam KinaseIIα
(1H7)
IgG2a
|
Acris Antibodies
Cat# H00000815-M02 |
Immunogen = partially recombinant CaMK2α with GST tag (AAH40457, aa 305-410) |
monoclonal mouse
(liquid, PBS, azide, purified) |
ELISA, WB |
CaMK2α |
human |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
Cam KinaseIIα
(2C11)
IgG2b
|
Acris Antibodies
Cat# H00000815-M04 |
Immunogen = partially recombinant CaMK2α with GST tag (AAH40457, aa 305-410) |
monoclonal mouse
(liquid, PBS, azide, purified) |
ELISA, WB |
CaMK2α |
human |
Cam KinaseIIα
(2C12)
IgG2b
|
Acris Antibodies
Cat# H00000815-M03 |
Immunogen = partially recombinant CaMK2α with GST tag (AAH40457, aa 305-410) |
monoclonal mouse
(liquid, PBS, azide, purified) |
ELISA, WB |
CaMK2α |
human |
Cam KinaseIIα
(2C4)
IgG2a
|
Acris Antibodies
Cat# H00000815-M01 |
Immunogen = partially recombinant CaMK2α with GST tag (AAH40457, aa 305-410) |
monoclonal mouse
(liquid, PBS, azide, purified) |
ELISA, WB |
CaMK2α |
human |
Cam KinaseIIα phos Thr286
(poly)
Ig
|
Acris Antibodies
Cat# BP7014 |
Immunogen = synthetic phospho-peptide derived from a region of human CaMKIIα that contains threonine 286 |
polyclonal rabbit
(liquid, PBS, BSA, glycerol, azide, affinity purified) |
WB |
CaMKIIα (50kDa, phospho-specific Thr286) |
human, rat, mouse |
CaMK2 α
(poly)
|
ABGENT
Cat# AP7206b |
Immunogen = synthetic peptide selected within aa 400-478 of human CAMK2α |
polyclonal rabbit
(liquid, PBS, azide, Protein G purified) |
ELISA, FC, IH(P), IP, WB |
CAMK2α |
human |
CaMK2 α
(poly)
|
VWR International, Inc.
Cat# 80083-906
supplied by Abgent |
Immunogen = synthetic peptide selected within aa 400-478 of human CAMK2α |
polyclonal rabbit
(liquid, PBS, azide, Protein G purified) |
ELISA, FC, IH(P), IP, WB |
CAMK2α |
human |
CaMK2 α, C-term
(poly)
|
AMS Biotechnology
Cat# AP7206b
supplied by Abgent |
Immunogen = synthetic peptide selected within aa 400~478 of human CAMK2α |
polyclonal rabbit
(liquid, PBS, azide, Protein G purified) |
ELISA, IH, WB |
CAMK2α |
human |
CaMK2 β
(poly)
|
ABGENT
Cat# AP7207a |
Immunogen = synthetic peptide selected within aa 500-550 of human CAMK2β. |
polyclonal rabbit
(liquid, PBS, azide, Protein G purified) |
ELISA, FC, IH(P), IP, WB |
CaMK2β |
human, primate (various) |
CaMK2 β
(poly)
|
VWR International, Inc.
Cat# 80083-908
supplied by Abgent |
Immunogen = synthetic peptide selected within aa 500-550 of human CAMK2β. |
polyclonal rabbit
(liquid, PBS, azide, Protein G purified) |
ELISA, FC, IH(P), IP, WB |
CaMK2β |
human, primate (various) |
CaMK2 β, C-term
(poly)
|
AMS Biotechnology
Cat# AP7207a
supplied by Abgent |
Immunogen = synthetic peptide selected within aa 500~550 of human CAMK2β. |
polyclonal rabbit
(liquid, PBS, azide, Protein G purified) |
ELISA, FC, IH(P), IP, WB |
CaMK2β |
human, primate (various) |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
CaMK2 γ
(poly)
|
ABGENT
Cat# AP7210a |
Immunogen = synthetic peptide selected from aa 397-411 of human CAMK2γ. |
polyclonal rabbit
(liquid, PBS, azide, Protein G purified) |
ELISA, FC, IH(P), IP, WB |
CAMK2γ. |
human |
CaMK2 γ
(poly)
|
VWR International, Inc.
Cat# 80083-914
supplied by Abgent |
Immunogen = synthetic peptide selected from aa 397-411 of human CAMK2γ. |
polyclonal rabbit
(liquid, PBS, azide, Protein G purified) |
ELISA, FC, IH(P), IP, WB |
CAMK2γ. |
human |
CaMK2 γ, C-term
(poly)
|
AMS Biotechnology
Cat# AP7210a
supplied by Abgent |
Immunogen = synthetic peptide selected from aa 397-411 of human CAMK2γ. |
polyclonal rabbit
(liquid, PBS, azide, Protein G purified) |
ELISA, FC, IH(P), IP, WB |
CAMK2γ. |
human |
CaMK2 δ
(poly)
|
ABGENT
Cat# AP7209a |
Immunogen = synthetic peptide selected within aa 450-499 of human CAMK2δ |
polyclonal rabbit
(liquid, PBS, azide, Protein G purified) |
ELISA, FC, IH(P), IP, WB |
CaMK2δ |
human, primate (various) |
CaMK2 δ
(poly)
|
VWR International, Inc.
Cat# 80083-912
supplied by Abgent |
Immunogen = synthetic peptide selected within aa 450-499 of human CAMK2δ |
polyclonal rabbit
(liquid, PBS, azide, Protein G purified) |
ELISA, FC, IH(P), IP, WB |
CaMK2δ |
human, primate (various) |
CaMK2 δ, C-term
(poly)
|
AMS Biotechnology
Cat# AP7209a
supplied by Abgent |
Immunogen = synthetic peptide selected within aa 450~499 of human CAMK2δ |
polyclonal rabbit
(liquid, PBS, azide, Protein G purified) |
ELISA, FC, IH(P), IP, WB |
CaMK2δ |
human, primate (various) |
CaMK2 δ-like
(poly)
|
ABGENT
Cat# AP7208a |
Immunogen = synthetic peptide selected from aa 324-338 of human CAMK2 δ-like |
polyclonal rabbit
(liquid, PBS, azide, Protein G purified) |
ELISA, FC, IH(P), IP, WB |
CaMK2δ-like |
human |
CaMK2 δ-like
(poly)
|
VWR International, Inc.
Cat# 80083-910
supplied by Abgent |
Immunogen = synthetic peptide selected from aa 324-338 of human CAMK2 δ-like |
polyclonal rabbit
(liquid, PBS, azide, Protein G purified) |
ELISA, FC, IH(P), IP, WB |
CaMK2δ-like |
human |
CaMK2 δ-like, C-term
(poly)
|
AMS Biotechnology
Cat# AP7208a
supplied by Abgent |
Immunogen = synthetic peptide selected from aa 324~338 of human CAMK2 δ-like |
polyclonal rabbit
(liquid, PBS, azide, Protein G purified) |
ELISA, FC, IH(P), IP, WB |
CaMK2δ-like |
human |
CaMK2α
(mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C21126
available in USA only |
Immunogen = synthetic thiophosphated (Thr286) peptide corresp. to aa 281-294 (M H R Q E T (PSO3) V D C L K K F N) from the α-subunit of mouse and rat CaM kinase II (Ca2+/ Calmodulin-dependent protein kinase II) |
monoclonal mouse
|
ELISA, IC, IP, WB |
recombinant and native phosphorylated -α subunit of rat CaM kinase II.
|
rat, mouse |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
CaMK2α
(mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C21190
available in USA only |
Immunogen = purified rat brain CaM Kinase |
monoclonal mouse
(DEAE-column chromatography) |
ELISA, IC, IP, WB |
α subunit of CaM Kinase II; no significant cross-reactivity was seen with the B, B1, γB, δA, δB and δC isoforms
|
human, rat, mouse, goldfish |
CaMK2α
(mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C21118
available in USA only |
Immunogen = partially purified rat CaM Kinase |
monoclonal mouse
(protein G purified) |
IH, RIA, WB |
phosphorylated and non-phosphorylated forms are recognized. Expected reactivity includes the α-, γ- and δ-isoforms, but not the β-isoforms |
rat, mouse, bovine |
CaMK2α
(mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C21119
available in USA only |
Immunogen = partially purified rat CaM kinase |
monoclonal mouse
|
IC, IP, WB |
phosphorylated and non-phosphorylated α-subunit of CaM kinase II |
rat |
CaMK2α
(mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C21186
available in USA only |
Immunogen = partially purified rat CaM kinase α-subunit |
monoclonal mouse
(protein G purified) |
ELISA, IH, WB |
α-subunit of CaM kinase II |
human, rat, bovine |
CaMK2α
(mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C21120
available in USA only |
Immunogen = purified type II CaM kinase |
monoclonal mouse
(protein A purified) |
IC, WB |
α-subunit of CaM kinase II |
rat, mammal (various), frog, chicken |
CaMK2α
(mono)
|
LifeSpan Biosciences, Inc.
Cat# LS-C21131
available in USA only |
Immunogen = partially purified rat CaM kinaseII |
monoclonal mouse
(protein G purified) |
IC, WB |
α-subunit of CaM kinase II (~50-60kDa) phosphorylated and non-phosphorylated forms; expect γ- and δ-subunits; not β-subunit |
rat, mouse, bovine |
CaMK2α
(poly)
|
LifeSpan Biosciences, Inc.
Cat# LS-C19073 |
Immunogen = synthetic peptide corresp. to aa 6-23 of human CaM Kinase II protein |
polyclonal rabbit
(affinity purified) |
ELISA, IF, WB |
CaM Kinase II |
human, rabbit, pig, zebra fish, frog |
CaMK2α
(poly)
|
LifeSpan Biosciences, Inc.
Cat# LS-C21117
available in USA only |
Immunogen = synthetic peptide corresp. to rat CaMK II synthetic peptide (aa. 7-20), conjugated to KLH |
polyclonal rabbit
(IgG fract.) |
ELISA, WB; not IP |
CaM Kinase II (60kDa) α-, γ- and δ-subunit; not the β-subunit |
rat |
CaMK2α
(poly)
|
LifeSpan Biosciences, Inc.
Cat# LS-C21124
available in USA only |
Immunogen = synthetic phospho-peptide derived from a region of human CaMKII that contains threonine 286 |
polyclonal rabbit
(unpurified serum) |
ELISA, WB |
CaM Kinase II (60kDa) |
human, rat, mouse |
CaMK2α
(poly)
|
LifeSpan Biosciences, Inc.
Cat# LS-C21125
available in USA only |
Immunogen = human CaMII phosphorylated synthetic peptide, corresp. to aa 281-288 and conjugated to KLH |
polyclonal rabbit
(DEAE-column chromatography) |
IP, WB |
CaM Kinase IIα (~60kDa and ~50kDa) |
human |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
CaMK2α
(poly)
|
LifeSpan Biosciences, Inc.
Cat# LS-C21122
available in USA only |
Immunogen = synthetic peptide around the N-terminal aa seq. of human CaMKII, (KLH coupled) |
polyclonal rabbit
(protein A purified) |
WB |
endogenous leels of total CaM Kinase II |
human |
CaMK2α phos Thr286
(poly)
|
LifeSpan Biosciences, Inc.
Cat# LS-C246 |
Immunogen = synthetic peptide |
polyclonal rabbit
(HEPES, NaCl, BSA, glycerol, affinity purified) |
WB |
CaM Kinase IIα and β phosphorylated at Thr286 |
human, rat, mouse |
CaMK2α phos Thr286
(poly)
|
LifeSpan Biosciences, Inc.
Cat# LS-C21121
available in USA only |
Immunogen = synthetic phospho-peptide corresp. to aa residues surrounding the phosphorylated Thr286 |
polyclonal rabbit
|
WB |
CaM Kinase II α- (50kDa) and β- (60kDa) subunits phosphorylated at Thr286 |
human |
CaMK2α phos Thr286
(poly)
|
LifeSpan Biosciences, Inc.
Cat# LS-C21123
available in USA only |
Immunogen = synthetic peptide corresp. to residues around threonine 286 of human CaMKII (KLH coupled) |
polyclonal rabbit
(protein A purified) |
ELISA, WB |
endogenous leels of CaM Kinase II only when phosphorylated at Threonine286 |
human, rat, mouse |
CaMK2α phos Thr286
(poly)
|
LifeSpan Biosciences, Inc.
Cat# LS-C21127
available in USA only |
Immunogen = synthetic peptide aa281-291 (K M H R Q E [pT] V D C L K) corresp. to rat calmodulin- dependent protein kinase II, subunit |
polyclonal rabbit
(protein A purified) |
WB |
phos-CaM Kinase II α- and β- subunits |
human, rat, mouse, porcine, drosophila, xenopus |
CaMK2α phos Thr286
(poly)
|
LifeSpan Biosciences, Inc.
Cat# LS-C21129
available in USA only |
Immunogen = synthetic phospho-peptide corresp. to an aa seq. within CAMKII which includes phosphorylated Thr 286 |
polyclonal rabbit
(DEAE-column chromatography) |
WB |
phos-CaM Kinase II α- and β- subunits when phosphorylated at Thr286 |
human, rat, mouse, xenopus |
CaMK2α phos Thr286
(poly)
|
LifeSpan Biosciences, Inc.
Cat# LS-C21188
available in USA only |
Immunogen = synthetic phospho-peptide corresp. to an aa seq. within CAMKII which includes phosphorylated Thr 286 |
polyclonal rabbit
(DEAE-column chromatography) |
WB |
recognizes the activated form of CaMKII (calmodulin-dependent protein kinase II) only when phosphorylated at Thr286
|
human |
CaMK2α phos Thr286
(poly)
|
LifeSpan Biosciences, Inc.
Cat# LS-C21189
available in USA only |
Immunogen = synthetic phospho-peptide corresp. to aa residues surrounding the phosphorylated Thr286 of CaM kinase II |
polyclonal rabbit
|
WB |
recognizes the activated form of CaMKII (calmodulin-dependent protein kinase II) only when phosphorylated at Thr286
|
human |
CaMK2α phos Thr305
(poly)
|
LifeSpan Biosciences, Inc.
Cat# LS-C247 |
Immunogen = synthetic peptide |
polyclonal rabbit
(HEPES, NaCl, BSA, glycerol, unpurified neat serum) |
WB |
CaM Kinase IIα and β phosphorylated at Thr305 |
human, rat, mouse, bovine, chicken |
CaMK2α phos Thr305
(poly)
|
LifeSpan Biosciences, Inc.
Cat# LS-C21128
available in USA only |
Immunogen = synthetic peptide corresp. to aa residues around phosphorylated Thr 305 within CAMKII |
polyclonal rabbit
(protein G purified) |
IH(F), WB |
CaM Kinase IIα and β when phosphorylated at Thr305 |
human, rat, mouse, xenopus |
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