|
Antibodies to Fibroblast Growth Factor (FGF)-related Antigens
|
| From the Manufacturers
|
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
aFGF
(poly)
|
American Qualex
Cat# R0340 |
|
polyclonal
|
|
FGF acidic |
human |
aFGF
(poly)
|
Antigenex
Cat #
RHF320 or
RHF320-B
(Biotin) |
immunogen = highly purified recombinant human FGF acidic |
polyclonal rabbit
(affinity purified, lyophilized) |
ELISA, Neut, WB |
FGF acidic |
human |
aFGF
(poly)
|
Biotrend
Cat# C-500P1-7
C-500P1-7L |
immunogen = highly pure (>98%) recomb. human FGF-acidic. |
polyclonal rabbit
(Ig fract, affinity purified, sterile filtered, PBS, lyophilized) |
ELISA, Neut, WB |
detects FGF acidic |
human |
aFGF
(poly)
|
Biotrend
Cat# C-500P1-7bt
C-500P1-7btL |
immunogen = highly pure (>98%) recomb. human FGF-acidic. |
polyclonal rabbit
(Ig fract, affinity purified, sterile filtered, PBS, biotin conj., lyophilized) |
ELISA, Neut, WB |
detects FGF acidic |
human |
aFGF
(poly)
IgG
|
Fitzgerald
Cat# 70-FR34
discontinued |
immunogen = highly pure (>98%) recombinant human FGF acidic |
polyclonal rabbit
(sterile filtered, PBS, no preservative, lyophilized) |
ELISA, Neut, WB |
detects FGF acidic; no reactivity to a cytokine panel |
human |
aFGF
(poly)
|
ID Labs Biotechnology
Cat# 311210500 |
immunogen = highly pure recombinant hFGF-acidic |
polyclonal rabbit
(sterile filtered, PBS, lyophilized) |
ELISA, Neut, WB |
FGF acidic |
human |
aFGF
(poly)
|
PeproTech
Cat# 500-P17 |
immunogen = highly pure recombinant human FGF acidic |
polyclonal rabbit
(serum, affinity purified, sterile filtered, lyophilized) |
ELISA, Neut, WB |
FGF acidic |
human |
aFGF
(poly)
|
PeproTech
Cat#
500-P17Bt |
Immunogen = recombinant human FGF-acidic (98% pure) |
polyclonal rabbit
(lyophilized, affinity purified, biotin labeled) |
ELISA, WB |
FGF-acidic |
human |
aFGF
(poly)
|
ProSci
Cat#
XP-5136 |
Immunogen = recombinant human FGF-acidic (>98% pure) |
polyclonal rabbit
(affinity purified, sterile filtered) |
ELISA, Neut, WB |
FGF-acidic |
human |
aFGF
(poly)
|
ProSci
Cat#
XP-5136BT |
Immunogen = recombinant human FGF-acidic (>98% pure) |
polyclonal rabbit
(lyophilized, affinity purified, sterile filtered, biotinylated) |
ELISA, WB |
FGF-acidic |
human |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
aFGF
(3117)
IgG1
|
R&D Systems
Cat #
MAB-232 |
immunogen = E. coli-derived recombinant human FGF acidic |
monoclonal mouse
(protein-G affinity purified, PBS, trehalose, lyophilized) |
ELISA, WB |
FGF acidic; no cross reactivity with other family members |
human |
aFGF
(poly)
IgG
|
R&D Systems
Cat #
AB-32NA |
immunogen = purified bovine FGF acidic |
polyclonal rabbit
(protein-A, PBS, lyophilized) |
ELISA, Neut, WB |
Bovine FGF acidic and human recombinant FGF acidic |
human, bovine |
aFGF
(poly)
IgG
|
R&D Systems
Cat #
AF-232 |
immunogen = E. coli-derived recombinant hFGF acidic |
polyclonal goat
(affinity purified, PBS, lyophilized) |
ELISA, Neut, WB |
rhFGF acidic; cross reacts with bovine FGF acidic and rhβ-ECGF |
human, bovine |
aFGF
(poly)
IgG
|
R&D Systems
Cat #
BAF-232 |
immunogen = E. coli-derived recombinant hFGF acidic |
polyclonal goat
(affinity purified, PBS, Biotin, lyophilized) |
WB |
Bovine FGF acidic and human recombinant FGF acidic |
human, bovine |
aFGF
(FA-88)
IgG2a
|
Sigma Aldrich
Cat# F9666 |
immunogen = recombinant human FGF acidic; epitope at or near receptor binding site of aFGF |
monoclonal mouse
(liquid, purified, sterile filtered, PBS) |
DB, ELISA, IH, Neut |
native and recombinant human FGF acidicic,human FGF-basic |
human, bovine |
aFGF
(poly)
IgG
|
Sigma Aldrich
Cat# F5521 |
immunogen = purified native bovine FGF acidic |
polyclonal rabbit
(protein-A, PBS, affinity purified, no preservative, lyophilized) |
ELISA, IB, Neut |
FGF acidic, not FGF basic |
human, bovine |
aFGF
(1.A.4)
IgM
|
US Biological
Cat #
F4210-04 |
immunogen = purified human recombinant acidic FGF expressed in yeast cells |
monoclonal mouse
(affinity purified, liquid, Tris) |
ELISA, WB |
FGF acidic |
human |
aFGF
(poly)
IgG
|
US Biological
Cat #
F4210-03 |
immunogen = highly purified recombinant antigen |
polyclonal rabbit
(affinity purified antiserum, PBS, Biotin, lyophilized) |
ELISA, Neut, WB |
detects FGF acidic |
human |
aFGF
(poly)
IgG
|
US Biological
Cat #
F4210-05A |
immunogen = highly purified human recombinant antigen |
polyclonal rabbit
(affinity purified antiserum, lyophilized) |
ELISA, WB |
FGF acidic |
human |
aFGF
(poly)
|
US Biological
Cat #
F4210-04B |
immunogen = human recombinant acidic FGF |
polyclonal rabbit
(affinity purified, liquid, Tris-HCl) |
ELISA, IC, WB |
FGF acidic |
human |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
aFGF
(1C10)
|
Wako Chemicals
Cat #
015-14221 |
|
monoclonal mouse
|
IC |
FGF acidic |
human |
aFGF
(AF1-114)
|
Wako Chemicals
Cat #
018-14211 |
|
monoclonal mouse
|
IC |
FGF acidic |
human |
aFGF
(AF1-52)
|
Wako Chemicals
Cat #
018-14191 |
|
monoclonal mouse
|
IC |
FGF acidic |
human |
aFGF
(AF1-81)
|
Wako Chemicals
Cat #
011-14201 |
|
monoclonal mouse
|
IC |
FGF acidic |
human |
aFGF
(poly)
|
Wako Chemicals
Peptide Institute,
Cat# Y260 |
|
polyclonal rabbit
(serum) |
|
aFGF (1-15) |
human |
aFGF /FGF-1
(poly)
|
Promokine
Cat# PK-AB815-60340 |
Immunogen = highly pure (>98%) recombinant hFGF-acidic |
polyclonal rabbit
(lyophilized, affinity purified, sterile filtered, PBS) |
ELISA, WB |
detects FGF acidic |
human |
basic FGF
(3)
IgG1
|
BD Transduction
Cat#
610871 |
Immunogen = full length human FGF basic |
monoclonal mouse
(liquid, buffered, BSA, glycerol, HRPO) |
IF, WB |
FGF basic, 18-24 kda by WB |
human, rat, mouse, dog, chicken |
bFGF
(F-3)
IgG1,κ
|
Abazyme
Cat #
MO-C40046H |
immunogen = purified recomb. human FGF basic |
monoclonal mouse
(protein-G affinity purified, PBS, lyophilized) |
ELISA |
FGF basic |
human
(others not tested) |
bFGF
(F-343)
IgG1,κ
|
Abazyme
Cat #
MO-C40046A |
immunogen = purified recomb. human FGF basic |
monoclonal mouse
(protein-G affinity purified, PBS, lyophilized) |
ELISA |
FGF basic |
human
(others not tested) |
bFGF
(F-538)
IgG1,κ
|
Abazyme
Cat #
MO-C40046G |
immunogen = purified recomb. human FGF basic |
monoclonal mouse
(protein-G affinity purified, PBS, lyophilized) |
ELISA |
FGF basic |
human
(others not tested) |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
bFGF
(poly)
|
ATS
Cat# AB07 |
Immunogen = amino acids 1-24 synthetic fragment of bovine FGF-2 |
polyclonal rabbit
(liquid, serum) |
IB, IH(P) |
FGF, basic |
human, mammal (various) |
bFGF
(poly)
|
ATS
Cat# AB08 |
Immunogen = amino acids 1-23 synthetic fragment of rat FGF-2 (1-46) |
polyclonal rabbit
(liquid, serum) |
IB, IH(P) |
FGF, basic |
rat, mammal (various) |
bFGF
(poly)
|
American Qualex
Cat# M8310 |
|
polyclonal
|
|
FGF basic |
human |
bFGF
(poly)
|
American Qualex
Cat# R0360 |
|
polyclonal
|
|
FGF basic |
human |
bFGF
(poly)
|
A R P
Cat#
11-PFGF-B |
immunogen = recombinant hFGF-basic |
polyclonal rabbit
(liquid, purified, sterile filtered, PBS) |
ELISA, Neut |
detects FGF basic |
human |
bFGF
(mono)
IgG1
|
Ab Solutions
Cat #
AS24-P |
immunogen = human recombinant fibroblast growth factor (hbFGF) |
monoclonal mouse
(liquid, Protein-A, PBS, sterile filtered, azide) |
ELISA, IH(P) |
FGF basic, not human acidic or a panel of other human cytokines |
human |
bFGF
(mono)
IgG1
|
Ab Solutions
Cat #
AS25-B |
immunogen = human recombinant fibroblast growth factor (hbFGF) |
monoclonal mouse
(liquid, Protein-A, PBS, sterile filtered, azide, Biotin conj) |
ELISA, IH(P) |
FGF basic, not human acidic or a panel of other human cytokines |
human |
bFGF
(mono)
IgG1
|
Ab Solutions
Cat #
AS25-P |
immunogen = human recombinant fibroblast growth factor (hbFGF) |
monoclonal mouse
(liquid, Protein-A, PBS, sterile filtered, azide, purified) |
ELISA, IH(P) |
FGF basic, not human acidic or a panel of other human cytokines |
human |
bFGF
(poly)
|
Antigenex
Cat #
RHF322 or
RHF322-B
(Biotin) |
immunogen = highly purified recombinant human FGF basic |
polyclonal rabbit
(affinity purified, lyophilized) |
ELISA, Neut, WB |
FGF basic |
human |
bFGF
(BDI306)
IgG1
|
Biodesign Meridian LifeScience
Cat# P14306M |
Immunogen = purified recomb. human FGF-basic |
monoclonal mouse
(protein-G purified, PBS, no preservative, lyophilized) |
ELISA |
FGF basic |
human |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
bFGF
(poly)
IgG
|
Biogenesis AdB Serotec
Cat# 4460-0104 |
Immunogen = purified recombinant hFGF-basic |
polyclonal rabbit
(purified Ig, liquid, PBS, azide) |
Neut, WB |
FGF basic, not acidic FGF |
human, rat, mouse, bovine |
bFGF
(poly)
IgG
|
Biogenesis AdB Serotec
Cat# AHP830 |
Immunogen = recombinant human FGF-basic |
polyclonal goat
(affinity purified IgG, PBS, azide, no preservative or stabilisers, lyophilized) |
ELISA, FA, WB |
recognizes human FGF basic |
human |
bFGF
(bFGF88)
IgG2b
|
BioGenex
Cat# AM359-5M |
Immunogen = synthetic peptide of FGF-basic coupled to KLH |
monoclonal mouse
(liquid, ascites diluted in PBS, BSA, azide) |
IH(P) |
detects basic FGF; can be used for the study of myometrial smooth muscle cells, uterine leiomyomas, cardiac myocytes, arterial endothelium, gastric carcinoma, and invasive or metastatic melanoma |
human |
bFGF
(poly)
IgG
|
Biomedical Technologies
Cat# BT583 |
immunogen = human recombinant bFGF |
polyclonal rabbit
(Ig fraction, purified) |
IH, WB; Inhibition |
FGF basic, not FGF acidic (WB) |
human, rat, mouse, bovine |
bFGF
(kit)
|
Biosource Invitrogen
Cat# LMG0021 |
|
(1. Antibody bead 10X concentrate solution 2. Biotin antibody concentrate 3. msGF std) |
ELISA |
FGF basic in serum, plasma,
buffered solution or tissue culture supernatant |
mouse |
bFGF
(kit)
|
Biosource Invitrogen
Cat# LHG0021 |
|
(1. Antibody bead 10x solution 2. Biotin antibody concentrate 3. hGF std) |
IF |
FGFb in serum, plasma, buffered solution or tissue culture supernat |
human |
bFGF
(poly)
IgG
|
Calbiochem EMD Biosciences
Cat# PC15 |
immunogen = synthetic peptide corr. to aa 147-153 of human bFGF |
polyclonal rabbit
(liquid, purified, PBS, azide, gelatin) |
ELISA, IH(P) |
recognizes bFGF, 18kDa protein in SK-HEP-1 cells and colon adenocarcinoma tissue, and may detect larger forms (22, 23 and 24 kDa) |
human |
bFGF
(poly)
IgG
|
Calbiochem EMD Biosciences
Cat# PC16 |
immunogen = synthetic peptide corr. to aa 40-63 of human bFGF |
polyclonal rabbit
(liquid, PBS, glycerol, no preservative) |
IH(P), WB |
recognizes bFGF, 18kDa protein, in fetal liver and kidney tissue |
human, rat |
bFGF
(mono)
IgG1
|
Chemicon and Upstate are Millipore
Cat# MAB120
Discontinued |
Immunogen = human FGF basic (18 kDa) |
monoclonal mouse
(purified, liquid, azide) |
IF, WB |
FGF basic |
human
(others not tested) |
bFGF
(4B5)
IgG2a
|
Chemicon and Upstate are Millipore
Cat# 04-531
produced by Upstate
Discontinued |
immunogen = recomb. protein encompassing aa. 303-408 of human full-length FGFR1 |
monoclonal mouse
(liquid protein-A purified) |
IF, WB |
FGFR1 (118 kDa) |
human
(others not tested) |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
bFGF
(AS24)
IgG1
|
Exalpha Biologicals
Cat# L210M |
immunogen = in vitro cell culture, protein G purified |
monoclonal mouse
(sterile filtered, PBS, azide) |
ELISA, IH |
basic FGF; not acidic FGF or a panel of other human cytokines |
human |
bFGF
(AS25)
IgG1
|
Exalpha Biologicals
Cat# L215M |
immunogen = in vitro cell culture, protein G purified |
monoclonal mouse
(sterile filtered, PBS, azide, Biotin) |
ELISA |
basic FGF; not acidic FGF or a panel of other human cytokines |
human |
bFGF
(mono)
IgG1,κ
|
PeproTech
Cat# 500-M38 |
Immunogen = recombinant human FGF-basic |
monoclonal mouse
(lyophilized, protein A purified) |
ELISA |
FGF-basic |
human |
bFGF
(poly)
|
PeproTech
Cat#
500-P18 |
immunogen = highly pure recombinant human FGF basic |
polyclonal rabbit
(serum, affinity purified, sterile filtered, lyophilized) |
ELISA, Neut, WB |
FGF basic |
human |
bFGF
(poly)
|
PeproTech
Cat#
500-P18Bt |
immunogen = highly pure recombinant human FGF basic |
polyclonal rabbit
(serum, affinity purified, sterile filtered, Biotin, lyophilized) |
ELISA, WB |
FGF basic |
human |
bFGF
(kit)
|
PeproTech
Cat#
900-K08 |
|
mouse
(anti-hFGF-basic as capture. Biotinylated rabbit anti-hFGF-basic as detection.) |
ELISA |
Natural or recombinant FGF-basic |
human |
bFGF
(mono)
IgG1,κ
|
ProSci
Cat#
XP-5718-M |
Immunogen = recombinant human FGF-basic |
monoclonal mouse
(lyophilized, Protein A purified from ascites) |
ELISA |
FGF-basic |
human |
bFGF
(poly)
|
ProSci
Cat#
XP-5137 |
Immunogen = recombinant human FGF-basic |
polyclonal rabbit
(lyophilized, affinity purified, sterile filtered) |
ELISA, Neut, WB |
FGF-basic |
human |
bFGF
(poly)
|
ProSci
Cat#
XP-5137BT |
Immunogen = recombinant human FGF-basic (98% pure) |
polyclonal rabbit
(lyophilized, affinity purified, sterile filtered) |
ELISA, WB |
FGF-basic |
human |
bFGF
(10043)
IgG2a
|
R&D Systems
Cat #
BAM-233 |
immunogen = E. coli-derived recombinant human FGF basic, aa 10 - 155 |
monoclonal mouse
(protein-G, PBS, BSA, Biotin, lyophilized) |
ELISA |
FGF basic |
human, bovine |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
bFGF
(10060)
IgG2b
|
R&D Systems
Cat #
MAB-233 |
immunogen = E. coli-derived recombinant human FGF basic |
monoclonal mouse
(protein-G, IgG fraction, PBS, trehalose, lyophilized) |
ELISA; capture |
FGF basic; no cross reactivity with other family members |
human |
bFGF
(poly)
IgG
|
R&D Systems
Cat #
AB-233-NA |
immunogen = purified, E. coli-derived, recomb. human fibroblast growth factor basic |
polyclonal goat
(protein-G, PBS, trehalose, lyophilized) |
ELISA, IH(P), Neut, WB |
detects FGF basic |
human, bovine |
bFGF
(poly)
IgG
|
R&D Systems
Cat #
AB-33-NA |
immunogen = purified bovine FGF basic |
polyclonal rabbit
(protein-A, PBS, trehalose, lyophilized) |
ELISA, Neut, WB |
detects bovine FGF basic and human recombinant FGF basic |
human, bovine |
bFGF
(poly)
IgG
|
R&D Systems
Cat #
AF-233-NA |
immunogen = purified, bovine FGF basic |
polyclonal goat
(affinity purified, PBS, trehalose, lyophilized) |
ELISA, IH(P), Neut, WB |
rhFGF basic and bovine FGF basic |
human, bovine |
bFGF
(poly)
IgG
|
R&D Systems
Cat #
BAF-233 |
immunogen = E. coli-derived recombinant human FGF basic |
polyclonal goat
(affinity purified, PBS, BSA, Biotin, lyophilized) |
IH(P), WB |
FGF basic; very low cross reaction with rhFGF acidic and rhFGF-7 |
human |
bFGF
(kit)
|
R&D Systems
Cat #
DY-233 |
|
mouse
(capture and detection antibodies, fhFGF standard) |
ELISA |
FGF basic, natural and recombinant; cell culture supernates and serum |
human |
bFGF
(kit)
|
R&D Systems
Cat #
DFB50 |
|
(Capture ab: mo anti-hFGF-basic Detection ab: mo anti-hFGF-basic conj HRP) |
ELISA |
detects FGF-basic (cell culture supernates, serum and plasma) |
human |
bFGF
(kit)
|
R&D Systems
Cat# HSFB100D |
|
(5.5 hour solid phase ELISA; contains E. coli-expressed recombinant human FGF basic and antibodies raised against the recombinant factor) |
ELISA |
FGF-basic (serum, plasma and urine) |
human |
bFGF
(MC-GF1)
IgG2a
|
AbD Serotec
Cat #
MCA-1400G |
immunogen = recombinant human basic FGF |
monoclonal mouse
(IgG, affinity purified, liquid, PBS, azide) |
IH(F) |
binds strongly to bFGF attached to proteo glycans, but is competitivelydisplaced by heparin; not for bFGF bound to cell surface receptors |
human |
bFGF
(poly)
IgG
|
AbD Serotec
Cat #
AHP-830 |
immunogen = highly purified recomb. human basic FGF |
polyclonal goat
(affinity purified, no preservative, PBS, lyophilized) |
ELISA, Neut, WB |
FGF basic |
human
(others not tested) |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
bFGF
(FB-8)
IgG1
|
Sigma Aldrich
Cat# F6162 |
immunogen = recombinant human FGF basic |
monoclonal mouse
(liquid, sterile filtered, PBS) |
DB, ELISA, IB, Neut, RIA |
native and recombinant human FGF basic,not human FGF-acidic |
human, bovine |
bFGF
(poly)
IgG
|
Sigma Aldrich
Cat# F3393 |
immunogen = synthetic peptides corr. to aa 1-24 of bovine FGF basic |
polyclonal rabbit
(sterile filtered, KLH, PBS, no preservative, lyophilized) |
ELISA, IH(P), Neut, WB |
FGF basic, not FGF acidic or BSA |
human, bovine |
bFGF
(poly)
IgG
|
Sigma Aldrich
Cat# F5537 |
immunogen = purified recomb. human FGF basic, expressed in E. coli |
polyclonal rabbit
(liquid IgG fraction, sterile filtered, PBS, azide) |
DB, IB, Neut, RIA |
detects FGF basic, not recomb. human FGF-acidic, IL-1α,IL-1β, IL-3 or TNF-α, nor with mouse or human IL-6 |
human |
bFGF
(3)
IgG1
|
BD Transduction
Cat#
610870 |
Immunogen = full length human FGF basic |
monoclonal mouse
(liquid, buffered, BSA, azide, glycerol) |
IF, WB |
FGF basic, 18-24 kda by WB |
human, rat, mouse, dog, chicken |
bFGF
(6)
IgG1
|
BD Transduction
Cat#
610072 |
Immunogen = full length human FGF basic |
monoclonal mouse
(liquid, buffered, BSA, glycerol, azide) |
IF, IH, IP, WB |
FGF basic, 18-24 kda by WB |
human, rat, mouse, dog, chicken |
bFGF
(6)
IgG1
|
BD Transduction
Cat#
610073 |
Immunogen = full length human FGF basic |
monoclonal mouse
(liquid, buffered, BSA, glycerol, azide) |
IF, IH, IP, WB |
FGF basic, 18-24 kda by WB |
human, rat, mouse, dog, chicken |
bFGF
(mono)
IgG
|
US Biological
Cat #
F4210-14J |
Immunogen = synthetic peptide (GFFLRIHPDGRVDGVREKSDPHIK), corresp. to amino acids 40-63 of Human fgf basic |
monoclonal rabbit
(liquid, buffer, glycerol) |
IH(P), WB |
detects FGF-basic |
human, rat |
bFGF
(0.T.49)
IgG1κ
|
US Biological
Cat #
F4210-21 |
immunogen = purified bovine brain basic FGF |
monoclonal mouse
(liquid, DEAE-52 purified, PBS, glycerol) |
IH(F, P), WB |
recognizes native and heat-inactivated bovine FGF basic; not bovine FGF acidic |
human, rat, mouse, bovine |
bFGF
(0.T.50)
IgG1κ
|
US Biological
Cat #
F4210-22 |
immunogen = purified bovine brain basic FGF |
monoclonal mouse
(liquid, DEAE purified, glycerol, TBS, no preservative) |
Neut; not WB |
recognizes non-denatured form of bovine basic fgf; no cross with bovine acidic fgf |
human, rat, mouse, bovine |
bFGF
(0.T.50)
IgG1κ
|
US Biological
Cat #
F4210-22X |
immunogen = purified bovine brain basic FGF |
monoclonal mouse
(liquid, DEAE purified, TBS, glycerol, no preservative) |
Neut; not WB |
recognizes non-denatured form of bovine basic fgf; no cross with bovine acidic fgf |
human, rat, mouse, bovine |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
bFGF
(1.A.3)
IgG1
|
US Biological
Cat #
F4210-14 |
immunogen = purified human recombinant basic FGF expressed in yeast cells |
monoclonal mouse
(affinity purified, liquid, PBS, glycerol) |
WB |
recognizes rhFGF basic; not rhFGF acidic |
human |
bFGF
(3H124)
IgG1,κ
|
US Biological
Cat #
F4210-14A |
immunogen = recombinant human basic FGF |
monoclonal mouse
(protein-G affinity purified, PBS, lyophilized) |
ELISA |
detects FGF-basic |
human |
bFGF
(3H125)
IgG1,κ
|
US Biological
Cat #
F4210-14B |
Immunogen = purified recombinant human FGF-basic |
monoclonal mouse
(lyophilized, Protein G purified, PBS) |
ELISA |
detects FGF-basic |
human |
bFGF
(3H126)
IgG1,κ
|
US Biological
Cat #
F4210-14C |
Immunogen = purified recombinant human FGF-basic |
monoclonal mouse
(lyophilized, Protien G purified, PBS) |
ELISA |
detects FGF-basic |
human |
bFGF
(3H127)
IgG1,κ
|
US Biological
Cat #
F4210-14D |
Immunogen = purified recombinant human FGF-basic |
monoclonal mouse
(lyophilized, Protein G purified, PBS) |
ELISA |
detects FGF-basic |
human |
bFGF
(3H128)
IgG1,κ
|
US Biological
Cat #
F4210-14E |
Immunogen = purified recombinant human FGF-basic |
monoclonal mouse
(lyophilized, Protein G purified, PBS) |
ELISA |
detects FGF-basic |
human |
bFGF
(3H129)
IgG2a,κ
|
US Biological
Cat #
F4210-14F |
Immunogen = purified recombinant human FGF-basic |
monoclonal mouse
(lyophilized, protein G purified, PBS) |
ELISA |
detects FGF-basic |
human |
bFGF
(3H130)
IgG2a,κ
|
US Biological
Cat #
F4210-14G |
Immunogen = purified recombinant human FGF-basic |
monoclonal mouse
(lyophilized, Protein G purified, PBS) |
ELISA |
detects FGF-basic |
human |
bFGF
(3H2133)
IgG2a
|
US Biological
Cat #
F4210-14H |
Immunogen = recombinant human FGF-basic |
monoclonal mouse
(liquid, protein G purified, PBS, azide) |
ELISA, IH(F) |
recognizes FGF-basic
Binds strongly to bFGF attached to proteoglycans, but is completely displaced by heparin.
Not suitable for detecting bFGF bound to cell surface receptors. |
human |
bFGF
(7F5)
IgG1,κ
|
US Biological
Cat #
F4210-15A |
immunogen = recombinant human fgf basic |
monoclonal mouse
(affinity purified lyophilized) |
ELISA |
FGF-basic, 100%, no reactivity to acytokine panel |
human |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
bFGF
(8k214)
IgG2a
|
US Biological
Cat #
F4210-14K |
immunogen = recombinant full length human protein |
monoclonal mouse
(liquid, affinity purified, PBS, azide) |
ELISA, IH(F) |
detects FGF basic; binds strongly to bfgf attached to proteoglycans, but is competitively displaced by heparin |
human |
bFGF
(poly)
IgG
|
US Biological
Cat #
F4210-12 |
immunogen = highly purified recombinant human FGF basic |
polyclonal rabbit
(affinity purified serum, PBS, Biotin, lyophilized) |
ELISA, WB |
detects FGF basic |
human |
bFGF
(poly)
IgG
|
US Biological
Cat #
F4210-15C |
immunogen = recombinant human FGF basic |
polyclonal rabbit
(sterile-filtered, PBS, lyophilized) |
ELISA, WB |
recognizes FGF-basic |
human |
bFGF
(poly)
IgG
|
US Biological
Cat #
F4210-13 |
immunogen = highly pure recombinant human FGF-basic |
polyclonal rabbit
(lyophilized PBS, affinity purified) |
ELISA, Neut, WB |
recognizes FGF-basic |
human |
bFGF
(poly)
IgG
|
US Biological
Cat #
F4210-13A |
Immunogen = recombinant human FGF-basic (highly pure) |
polyclonal rabbit
(lyophilized PBS, affinity purified, biotinylated) |
ELISA, WB |
recognizes FGF-basic |
human |
bFGF
(poly)
IgG
|
US Biological
Cat #
F4210-11A |
immunogen = highly purified recombinant basic FGF |
polyclonal rabbit
(affinity purified, PBS, lyophilized) |
ELISA, WB |
recognizes FGF-basic |
human |
bFGF
(bFM-1)
IgG1κ
|
Upstate and Chemicon are Millipore
Cat# 05-117 |
immunogen = purified bovine brain basic FGF |
monoclonal mouse
(purified, PBS, sterilized) |
Neut, RIA |
basic FGF; no cross reactivity with bovine acidic FGF |
human, rat, mouse, bovine |
bFGF
(bFM-2)
IgG1κ
|
Upstate and Chemicon are Millipore
Cat# 05-118 |
immunogen = purified bovine brain basic FGF |
monoclonal mouse
(purified, PBS, sterilized) |
IH(F, P), WB |
basic FGF, 17.5 kDa; no cross reactivity with bovine acidic FGF |
human, rat, mouse, bovine |
bFGF
(3H3)
IgG1
|
Wako Chemicals
Cat #
014-14311 |
immunogen = human bFGF, mutein CS23 |
monoclonal mouse
(ascites, affinity purified, BSA, PBS, azide, lyophilized) |
ELISA, IC, WB |
FGF basic; no cross reaction with FGF acidic or HST1 |
human |
bFGF
(3H3)
IgG1
|
Wako Chemicals
Cat #
013-14641 |
immunogen = human bFGF, mutein CS23 |
monoclonal mouse
(affinity purified, peroxidase, Kathon, BSA, PBS, lyophilized) |
IC |
FGF basic |
human |
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