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Home
:
Research
:
Compendia
:
Antibodies
Antibody Directory
Initial Search
Antigen Class:
-- all --
14-3-3
4E-BP1
4-hydroxy-nonenal
5-HT /serotonin
ABC, ABC transporter
ACC
ACE
Acetylcholine-related
Actin related
ADAM and ADAMTS
Adaptin
Adaptor Protein Complex (AP)
Adenomatus Polyposis Coli /APC
Adenylate cyclase
AFP
AGE and RAGE
Akt and serine/threonine kinases
Alix
Alpha-1-antitrypsin
α-, β-, γ-secretase
AMCase
Amino acids, phosphorylated
AMPAR, NMDAR, GluR1, GluR2
Amphiphysin
AMPK
Amyloid-beta related
Annexin II
AP-2 transcription factor
Apolipoproteins
APP, APLP
Arc
ASCL1/ASH1/MASH1
ASK
ATPase
Bad
Barrier-to-autointegration factor /BAF
Bassoon
Bax
Bcl and BAG
BDNF, pro-BDNF
β-Catenin related
β-galactosidase
Biotin
BiP and GRP78/KDEL
B-Lymphocyte
BrdU
BRSK
Bruchpilot
BSA
Cadherin
Calbindin & Calretinin
Calcium Channel
Calmodulin
Calnexin
Calpain
Calpastatin
Calponin
Calreticulin
calsyntenin
CaM Kinase II
Casein Kinase
CASK
Caspase
Cathepsin
Caveolin and GLUT
CD11b, Integrins
CD147
CD40L
CD45
CDK1/CDC2 and CDK2
CDK4, CDK5 and p25/35
CD_ except CD11b, CD40, CD45 and CD147
c-Fos and c-Jun
Chemokine receptor
CHIP
Charged multivesicular body protein /CHMP
Chromogranin
Chx
CI-MPR
Clathrin
CNPase
Cofilin
Collagen
Complement proteins
Connexin
COX1, COX2, COX3
Cre Recombinase
CREB, cAMP and CBP
CRF
Cyclins A, B, B1 and D2
CYP46A1
Cystatin C
Cytochrome C
DCC
DCP
DDX6 /RCK
Desmin and Vimentin
Digoxigenin
DJ-1
Dlg/Discs-large
Dlx2
DNA/RNA Oxidative Damage
Dopamine related: DBH, DRD, DDC
Doublecortin
DR6
Drebrin
Dynamin
Dynamin related protein
Dynein
eIF /Eukaryotic initiation factor
ECE
ECT, Lipid Metabolism
EEA1
EGF, EGFr
EGR-1 /Zif268
Elav proteins
Elongation Factors
Endosomes
ERAB
Erythroid cells
Exocyst Complex
F4/80
FADD
FAS-Related
F-box
Fe65
FGF, FGFR
Fibroblasts
FKBP12
FKHR/FoxO proteins
FLAG, ECS and other tags
Flotillin
Fragile-X /FMRP
Frizzled related
Furin Convertase
Futsch
GABA related and Gephyrin
GAD65/67
GalR, Substance P
Gangliosides
GAP related
GAPDH /G3PDH
GATA
GFAP
GFP and RFP
GITR
glucocorticoid receptor /GR
Glutamine Synthetase
Go
Golgi matrix/membrane, GM130
Grb-2
GRKs
GSK-3
GST
HA and HIS peptides
Hb9
Heat Shock Proteins
Heme Oxygenase, Peroxiredoxin
Heparan Sulfate, proteoglycans, MSRA
HES
Histone deacetylase (HDAC)
histones
hnRNP
Homer
Hox
Human nucleii
Huntingtin
Huntington's-related
Iba-1
IFN-γ
IGF Receptor
IgG
Insulin Receptor
Interleukins
IRF
iron metabolism: transferrin, ferroportin, CP
IRS
Islet
JAK2
JNK/SAPK-1/2/3 and MAPK8-10
Ki-67
Kinesin Heavy Chain
Lamin and Nuclear Pore Complex
Laminin
Lamp
Lck and Lck Ligand
LDL Receptor family
LHX2
LL37 /CAP18
LRRK2
LTE4
Ly-6 surface proteins
MAPK, ERK
MAPKAPK/MAPK activated kinase
MAPs
Matrix Metalloproteinase (MMP)
MeCP2
MEK/MAP2K
MEKK/MAP3K and MAPKAP1
MEKKK/MAP4K
Melanocyte Stimulating Hormone
Met-Enkephalin
MHC related proteins
Mint
Mitochondria related
MKK
Monocytes
mTOR-related
myc and V5 epitope tags
Myelin Basic Protein
Myocardin
Myosin
Nanog
NCP1
NDUFS4
Neprilysin /CD10
Nestin, Neural Progenitors
NeuN
Neurexin
Neuro D
Neurofib Tangles and p62
Neurofilament
Neurogenin
Neuropeptide Y
Neurophysin Related
NFkB, IkB and related proteins
NG2
NGF, NGF-related
NGFR, Trk-A/B
Nieman Pick C1
Nitrotyrosine
Nkx
NOS
Notch, Jagged, Delta
NOX2
NSE
NUMB and NUMBLike
Occludin
O-GlcNAc
Olfactory Proteins
Oligodendrocyte markers
Optineurin
Orexin
p19
p21 and p27
p38 MAPK and MAPK11-14
p53
PAK
PARP
Parvalbumin
PAX6
PCAF
PCNA
PCTAIRE
PDGF, PDGF Receptor
PGC
Phosphorylated Strings
PIAS3
PINK1
PIP
PKA, PKC and related
Plaque-related
Potassium channel, voltage gated
Presenilin Complex, Nicastrin, Ubiquilin
Presenilins
Prion protein
Progranulin /PCDGF
Proline disulfide isomerase /PDI
Proteasome related
Protein Phosphatase related
proteolipid protein /PLP
PSA-NCAM
PSD95
PTPs
Rab or Ras related, except Rab3
Rac and Cdc42
RAP
Recoverin
Reelin
Retrolinkin
Retromer Complex
Rho
Rhodopsin
Ribonucleotide Reductase
Ryanodine receptor
S-100
S6 Kinase
Sestrin
SHP2
SIRT1/Sir2 and SIRT2
SNX
SOCS3/CIS3
SOD
Sodium channel, voltage gated
Somatostatin related
Sortilins
SOS-1
Sox
Spectrins
Splicing Factors
SR-BI
Src
SREBP
SRF
SSEA
Stat1, Stat3 and related proteins
Sulfiredoxin
SUMO
Synaptic Vesicle proteins
Synaptosomal proteins
Synucleins
TACE
TAR DNA-binding protein /TDP-43
Tau
Tbr
TdT
TGF
Thioredoxin related
TIA related
Tip60
TLR /Toll-like Receptor
TMP21
TNF
Tom
tPA and PAI-1
TRA-1
Trans-Golgi network /TGN
TRRAP
Tryptophan Hydroxylase
TSG101
Tubulin
TUC (TOAD/Ulip/CRMP)
Tyrosine Hydroxylase
Tyrosine kinase
UbcH
Ubiquitin
Urocortin
VAChT
Vav
VCAM
VDAC /Porin
VEGF and VEGF Receptor
Veli
VGAT
vGluT
von Willebrand factor
Vps35
WNT3
ZO-1
(plus)
Keywords:
any keywords
all keywords
Search Results:
Antigen Class
=14-3-3
Narrow Results
Host:
Method:
Species Reactivity:
Distributor:
-- select --
chicken
goat
mouse
rabbit
sheep
----------------
all monoclonal
all polyclonal
-- select --
ELISA
FC
IC
IF
IH
IH(P)
IP
WB
and
-- select --
Arabidopsis thaliana
bovine
C. elegans
chicken
cow
dog
human
mammal (various)
monkey
mouse
pig
rabbit
rat
sheep
various vertebrate
xenopus
and
-- select --
Abcam Ltd
AnaSpec, Inc
Biolegend, Inc.
LifeSpan Biosciences
Novus Biologicals, I
SANTA CRUZ BIOTECHNO
United States Biolog
exclude selected host
-- select --
ELISA
FC
IC
IF
IH
IH(P)
IP
WB
-- select --
Arabidopsis thaliana
bovine
C. elegans
chicken
cow
dog
human
mammal (various)
monkey
mouse
pig
rabbit
rat
sheep
various vertebrate
xenopus
1 to 100 of 136 results
Important Notice:
We are striving to include as many companies or products as possible, but we do not make a claim to being comprehensive. We may inadvertently omit specific companies or products due to a lack of information.
Antibodies to 14-3-3 proteins
From the Manufacturers
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity
Reactivity
top
14-3-3
(3F7)
IgG
1
, κ
Abcam
Cat# ab14108
immunogen = recombinant full length human 14-3-3 gamma protein
monoclonal mouse
(liquid, PBS, BSA, azide, protein G purified)
IP, WB
14-3-3 protein
human
14-3-3
(poly)
IgG
Abcam
Cat# ab9063
immunogen = a mixture of 14-3-3 fusion proteins (beta, eta, tau, and sigma)
polyclonal rabbit
(liquid, whole antiserum, PBS, azide, BSA, thimerisol)
IP, WB
14-3-3 isoforms
human, rat, mouse
(all mammals)
14-3-3
(poly)
IgG
Abcam
Cat# ab6081
immunogen = synthetic peptide with the sequence D K S E L V Q K A K L A E Q A E R Y D, mapping to the N-terminal domain of human 14-3-3
polyclonal rabbit
(liquid, PBS, azide, gelatin, affinity purified)
IH(P)
, IP, WB
14-3-3 isoforms (alpha, beta, zeta, and theta)
human, rat, mouse
14-3-3
(poly)
IgG
Abcam
Cat# ab14112
immunogen = 20 aa synthetic peptide derived from the N-terminus of the human 14-3-3 beta/alpha protein
polyclonal rabbit
(liquid, PBS, azide, affinity purified)
ELISA, WB
broadly reactive with members of the 14-3-3 family of proteins (~29–32kDa)
human, rat, mouse, cow, C. elegans, xenopus
14-3-3
(poly)
IgG
Abcam
Cat# ab30579
immunogen = synthetic peptide corresp. to aa residues from the C-terminal region of human 14-3-3 protein, conj. to KLH
polyclonal rabbit
(liquid, HEPES, BSA, NaCl, no preservative, glycerol, affinity purified)
WB
14-3-3 protein
human, rat, mouse, xenopus
14-3-3
(poly)
IgY
Abcam
Cat# ab59143
immunogen = full length
E coli
-derived fusion protein
polyclonal chicken
(liquid, PBS, no preservative, affinity purified)
WB
14-3-3 protein
human, rat, mouse
14-3-3
(poly)
IgG
Abcam
Cat# ab35364
immunogen = full length native 14-3-3 protein purified from human brain
polyclonal sheep
(liquid, PBS, azide, protein G purified)
WB
recognises the lower molecular weight subunit (26 kDa) of 14-3-3
human
(all mammals)
14-3-3 (aC-12)
(poly)
IgG
Santa Cruz
Cat# sc-12672
Cat# sc-12672 P (peptide)
immunogen = epitope mapping near the C-terminus of Arabidopsis thaliana 14-3-3
polyclonal goat
(liquid, PBS, azide, gelatin, affinity purified)
ELISA, IF, WB
14-3-3
Arabidopsis thaliana
14-3-3 (at-82)
(poly)
IgG
Santa Cruz
Cat# sc-33752
immunogen = epitope corresp. to aa 21-75 mapping within an internal region of Arabidopsis thaliana 14-3-3
polyclonal rabbit
(liquid, PBS, azide, gelatin)
ELISA, IF, IP, WB
14-3-3 and to a lesser extent 14-3-3-like proteins
Arabidopsis thaliana
14-3-3 (B-11), pan
(mono)
IgG
1
Santa Cruz
Cat# sc-133232
immunogen = raised against aa 1-246 representing full length human 14-3-3β
monoclonal mouse
(liquid, PBS, azide, gelatin)
ELISA, IF, IP, WB
14-3-3
human, rat, mouse
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity
Reactivity
top
14-3-3 (B-8), pan
(mono)
IgG
1
Santa Cruz
Cat# sc-133233
immunogen = raised against aa 1-246 representing full length human 14-3-3β
monoclonal mouse
(liquid, PBS, azide, gelatin)
ELISA, IF, IP, WB
14-3-3
human, rat, mouse
14-3-3 (Fl-246), pan
(mono)
IgG
1
Santa Cruz
Cat# sc-13959
immunogen = raised against aa 1-246 representing full length human 14-3-3β
monoclonal mouse
(liquid, PBS, azide, gelatin)
ELISA, IF, IP, WB
14-3-3 protein family
human, rat, mouse
14-3-3 (K-19), pan
(poly)
IgG
Santa Cruz
Cat# sc-629
Cat# sc-629 P (peptide)
Cat# sc-629 AC (agarose)
Cat# sc-629 PE
Cat# sc-629 AF405 (Alexa Fluor® 405)
Cat# sc-629 AF488 (Alexa Fluor® 488)
Cat# sc-629 AF647 (Alexa Fluor® 647)
immunogen = epitope mapping at the N-terminus of human 14-3-3β
polyclonal rabbit
(liquid, PBS, azide, gelatin, affinity purified)
ELISA, FC, IF,
IH(P)
, IP, WB
detects members of 14-3-3 protein family
human, rat, mouse
14-3-3 (K-19), pan
(poly)
IgG
Santa Cruz
Cat# sc-629 PE
Cat# sc-629 AF405 (Alexa Fluor® 405)
Cat# sc-629 AF488 (Alexa Fluor® 488)
Cat# sc-629 AF647 (Alexa Fluor® 647)
immunogen = epitope mapping at the N-terminus of human 14-3-3β
polyclonal rabbit
(liquid, PBS, azide, gelatin, affinity purified)
FC, IF
detects members of 14-3-3 protein family
human, rat, mouse
14-3-3 (K-19), pan
(poly)
IgG
Santa Cruz
Cat# sc-629
Cat# sc-629 P (peptide)
Cat# sc-629 AC (agarose)
immunogen = epitope mapping at the N-terminus of human 14-3-3β
polyclonal rabbit
(liquid, PBS, azide, gelatin, affinity purified)
ELISA,
IH(P)
, IP, WB
detects members of 14-3-3 protein family
human, rat, mouse
14-3-3 Binding Domain (phos S)
(poly)
IgG
Abcam
Cat# ab14127
immunogen = synthetic phospho (Ser) 14-3-3-binding-motif peptides
polyclonal rabbit
(liquid, HEPES, BSA, NaCl, no preservative, glycerol, protein A purified)
ELISA,
IH(P)
, IP, WB
binds peptides and proteins containing a motif composed of phospho-Ser with proline at the +2 position and arginine or lysine at the -3 position
14-3-3 binding motif, phos Ser
(3i2)
IgG
1
US Biological
Cat# 0014-30
immunogen = synthetic phospho-(Ser) 14-3-3 binding motif peptides (KLH coupled)
monoclonal mouse
(liquid, ascites, HEPES, NaCl, BSA, glycerol, azide)
ELISA, IP, WB
wide range of peptides containing this phosphorylated 14-3-3 binding motif;
no
cross-reaction with corresponding non-phosphorylated sequences
14-3-3 binding motif, phos Ser
(poly)
IgG
US Biological
Cat# 0014-33
immunogen = synthetic phospho-(Ser) 14-3-3 binding motif peptides (KLH coupled)
polyclonal rabbit
(liquid, HEPES, NaCl, BSA, glycerol, affinity purified)
ELISA,
IH(P)
, IP, WB
binds peptides and proteins containing a motif composed of phospho-Ser with proline at the +2 position and arginine or lysine at the -3 position;
no
cross-reaction with corresp. non-phosphorylated sequences or with other phospho-Thr/Ser/Tyr -containing motifs
14-3-3 pan
(2Q248)
IgG
2a
Abcam
Cat# ab14121
immunogen = synthetic peptide, corresp. to aa208-244 of rat 14-3-3
monoclonal mouse
(liquid, supernatant, no preservative)
WB
14-3-3 protein (33 kDa)
rat
14-3-3 pan
(mono)
IgG
1
, κ
AnaSpec
Cat# 53202
immunogen = 14.3.3 gamma recombinant protein of human origin
monoclonal mouse
(protein A purified, PBS, BSA, azide)
IP, WB
recognizes 14-3-3
human
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity
Reactivity
top
14-3-3 phos Ser
(3i2)
IgG
1
Abcam
Cat# ab14126
immunogen = synthetic phospho(Ser) 14-3-3 binding motif peptides (KLH coupled)
monoclonal mouse
(liquid, HEPES, NaCl, glycerol, BSA, no preservative, protein A purified)
ELISA, IP, WB
binds peptides/ proteins containing phospho-Ser surrounded by Pro at the +2 position and Arg/Lys at the -3 position.
No
crossreactivity is observed with corresp. nonphosphorylated seq.
14-3-3 phos Ser58
(poly)
IgG
Abcam
Cat# ab30554
immunogen = phosphopeptide corresp. to aa residues surrounding the phosphorylation site Ser58 of rat 14-3-3 protein
polyclonal rabbit
(liquid, HEPES, BSA, NaCl, no preservative, glycerol, affinity purified)
WB
specific for the ~29k 14-3-3 protein phosphorylated at Ser58
human, rat
(expect mouse and xenopus due to seq. homology)
14-3-3α/β
(22-IID8B)
IgG
Abcam
Cat# ab12347
immunogen = lysed transformed amnion cells (AMA) (human)
monoclonal rabbit
(liquid, PBS, PMSF, glycerol, no preservative, protein G purified)
IC,
IH(P)
, WB
specific for 14-3-3 alpha + beta
monkey, human, rat, mouse, rabbit, pig, sheep, dog, cow
14-3-3α/β
(E58)
IgG
Abcam
Cat# ab32377
immunogen = synthetic peptide corresp. to residues in the N-terminal of human 14-3-3 alpha + beta
monoclonal rabbit
(liquid, Ig fract., tris-glycine, NaCl, BSA, glycerol, azide)
FC,
IH(P)
, WB;
not
IC or IP
specific for 14-3-3 alpha + beta
human, rat, mouse
(expect cow and sheep due to seq. homology)
14-3-3α/β
(Y62)
IgG
Abcam
Cat# ab32560
immunogen = synthetic peptide corresp. to residues in the C-terminal of human 14-3-3 alpha + beta
monoclonal rabbit
(liquid, tris-glycine, NaCl, BSA, glycerol, azide)
FC, IC,
IH(P)
, IP, WB
specific for 14-3-3 alpha + beta
human, rat, mouse
14-3-3α/β
(poly)
IgG
Abcam
Cat# ab37550
immunogen = synthetic peptide selected within aa 200~300 of human 14-3-3 beta, conj. KLH
polyclonal rabbit
(liquid, PBS, azide, protein G purified)
ELISA,
IH(P)
, WB
binds both transcript variants of 14-3-3 (alpha and beta)
human
14-3-3α/β
(poly)
IgG
Abcam
Cat# ab37551
immunogen = synthetic peptide selected within aa 50~150 of human 14-3-3 beta (N-terminal), conj. KLH
polyclonal rabbit
(liquid, PBS, azide, protein G purified)
ELISA,
IH(P)
, WB
binds to both isoforms of 14-3-3 (alpha and beta)
human
14-3-3α/β
(poly)
IgG
Abcam
Cat# ab52995
immunogen = synthetic peptide derived from the N-terminus of human 14-3-3 alpha/beta protein
polyclonal rabbit
(liquid, PBS, azide, affinity purified)
ELISA, WB
broadly reactive with the ~29-32 kDa members of the 14-3-3 family of proteins
human, rat, mouse, cow, C. elegans, xenopus
14-3-3β
(4E1)
IgG
2b
, κ
Abcam
Cat# ab12341
immunogen = recombinant full length rat protein
monoclonal mouse
(liquid, PBS, azide, sucrose, protein A purified)
IP, WB
14-3-3beta
rat, mouse
14-3-3β
(60C10)
IgG
2b
, κ
Abcam
Cat# ab16859
immunogen = recombinant full length human protein
monoclonal mouse
(liquid, PBS, no preservative, glycerol, protein A purified)
ELISA, WB
14-3-3beta
human, rat, mouse
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity
Reactivity
top
14-3-3β
(poly)
IgG
Abcam
Cat# ab15260
immunogen = synthetic peptide (unfortunately, the aa seq. is considered to be commercially sensitive) (human) (N-terminal)
polyclonal rabbit
(liquid, PBS, azide, BSA, affinity purified)
IH(P)
, IP, WB
14-3-3beta
human, rat, mouse
14-3-3β
(poly)
IgG
Abcam
Cat# ab16730
immunogen = recombinant full length protein (human)
polyclonal rabbit
(liquid, PBS, no preservative, glycerol, protein A purified)
WB
14-3-3beta
human, rat, mouse
14-3-3β
(poly)
IgG
Abcam
Cat# ab15262
immunogen = synthetic peptide (unfortunately, the aa seq. is considered to be commercially sensitive) (human) (N-terminal)
polyclonal rabbit
(liquid, PBS, azide, BSA, affinity purified, prediluted)
IH(P)
14-3-3beta
human, rat, mouse
14-3-3β
(poly)
AnaSpec
Cat# 53203
immunogen = a synthetic peptide corresponding to a protein sequence close to N-terminus of human 14-3-3β
polyclonal rabbit
(epitope specific, affinity purified, PBS, BSA, azide)
IH(P)
, IP, WB
14-3-3beta
human, rat, mouse
14-3-3β (4E1)
(mono)
IgG
2b
Santa Cruz
Cat# sc-59417
immunogen = raised against recombinant rat 14-3-3β
monoclonal mouse
(liquid, PBS, azide, gelatin)
IP, WB
14-3-3β
rat, mouse
14-3-3β (60C10)
(mono)
IgG
2b
Santa Cruz
Cat# sc-59419
immunogen = raised against recombinant human 14-3-3β
monoclonal mouse
(liquid, PBS, azide, gelatin)
IP, WB
14-3-3β
human, rat, mouse
14-3-3β (A-15)
(poly)
IgG
Santa Cruz
Cat# sc-17288
Cat# sc-17288 P (peptide)
immunogen = epitope mapping within an internal region of human 14-3-3
polyclonal goat
(liquid, PBS, azide, gelatin)
ELISA, IF,
IH(P)
, IP, WB
14-3-3 β
human, rat, mouse
14-3-3β (A-6)
(mono)
IgG
2b
Santa Cruz
Cat# sc-25276
Cat# sc-25276 P (peptide)
Cat# sc-25276 AC (agarose)
immunogen = epitope mapping at the C-terminus of human 14-3-3
monoclonal mouse
(liquid, PBS, azide, gelatin)
ELISA, IF,
IH(P)
, IP, WB
14-3-3β
human, rat, mouse
14-3-3β (C-20)
(poly)
IgG
Santa Cruz
Cat# sc-628
Cat# sc-628 P (peptide)
Cat# sc-628 FITC
Cat# sc-628 TRITC
Reference:
Emmanouilidou, 2010
immunogen = epitope mapping at the C-terminus of human 14-3-3β
polyclonal rabbit
(liquid, PBS, azide, gelatin)
ELISA, IF,
IH(P)
, IP, WB
14-3-3β and, to a lesser extent, other 14-3-3 family members
human, rat, mouse, cow, mammal (various)
14-3-3β (H-8)
(mono)
IgG
2b
Santa Cruz
Cat# sc-1657
Cat# sc-1657 P (peptide)
Cat# sc-1657 AC (agarose)
Cat# sc-1657 HRP
immunogen = epitope mapping at the N-terminus of human 14-3-3β
monoclonal mouse
(liquid, PBS, azide, gelatin)
ELISA, FC, IF,
IH(P)
, IP, WB
detects members of the 14-3-3 protein family
human, rat, mouse
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity
Reactivity
top
14-3-3β (H-8)
(mono)
IgG
2b
Santa Cruz
Cat# sc-1657 PE
Cat# sc-1657 FITC
Cat# sc-1657 TRITC
Cat# sc-1657 AF405 (Alexa Fluor® 405)
Cat# sc-1657 AF405 (Alexa Fluor® 488)
Cat# sc-1657 AF647 (Alexa Fluor® 647)
immunogen = epitope mapping at the N-terminus of human 14-3-3β
monoclonal mouse
(liquid, PBS, azide, gelatin)
FC, IF
detects members of the 14-3-3 protein family
human, rat, mouse
14-3-3β/ε/ζ (3C8)
(mono)
IgG
2b
Santa Cruz
Cat# sc-59420
immunogen = raised against human 14-3-3β fusion protein
monoclonal mouse
(liquid, PBS, azide, gelatin)
IP, WB
14-3-3β/ε/ζ (beta, epsilon, zeta)
human, rat, mouse
14-3-3β/ζ
(4E2)
IgG
Abcam
Cat# ab14114
immunogen = tissue / cell preparation (lysed transformed human amnion cells)
monoclonal mouse
(liquid, PBS, no preservative, glycerol, PMSF, protein G purified)
IC, WB
14-3-3beta/zeta
monkey, human, rat, mouse, pig, sheep, dog, cow
14-3-3β/ζ phos ser185
(poly)
IgG
Abcam
Cat# ab79166
immunogen = synthetic phosphopeptide corresp. to aa surrounding phospho Serine185
polyclonal sheep
(liquid, BSA, azide, glycerol, TBS, affinity purified)
WB
recognizes 14-3-3 phospho Serine185. It is specific for the phosphorylated form of beta and zeta 14-3-3 that are found endogenously phosphorylated only in brain tissue
human, rat, mouse, sheep, cow
14-3-3γ
(mono)
IgG
2b
, κ
Abcam
Cat# ab58071
immunogen = recombinant fragment corresp. to aa70-170 of human 14-3-3 gamma
monoclonal mouse
(liquid, PBS, no preservative, protein G purified)
WB
14-3-3γ
human
14-3-3γ
(3F7)
IgG
1
, κ
Abcam
Cat# ab14110
immunogen = recombinant full length human 14-3-3 gamma protein
monoclonal mouse
(liquid, PBS, no preservative, no BSA, protein G purified)
IC, IF, IP, WB
14-3-3 protein (beta, gamma and zeta isoforms)
human, rat
14-3-3γ
(3F8)
IgG
1
, κ
Abcam
Cat# ab14118
immunogen = recombinant full length human 14-3-3 gamma protein
monoclonal mouse
(liquid, PBS, no preservative, protein G purified)
IP, WB
14-3-3γ;
no
crossreactivity with other isoforms of 14-3-3
human
14-3-3γ
(3F8)
IgG, κ
Abcam
Cat# ab14117
immunogen = recombinant full length human 14-3-3 gamma protein
monoclonal mouse
(liquid, PBS, BSA, azide, protein G purified)
IP, WB
recognizes both zeta and gamma isoforms. This antibody shows no crossreactivity with other isoforms of 14-3-3
human
14-3-3γ
(CG31)
IgG
1
, κ
Abcam
Cat# ab76525
immunogen = recombinant full length human 14-3-3 gamma protein
monoclonal mouse
(liquid, PBS, no preservative, no BSA, protein G purified)
IP, WB
14-3-3γ;
no
crossreactivity with other isoforms of 14-3-3
human
14-3-3γ
(poly)
IgG
Abcam
Cat# ab69592
immunogen = synthetic peptide derived from internal of human 14-3-3 gamma
polyclonal rabbit
(liquid, PBS, NaCl, azide, glycerol, affinity purified)
ELISA, IC, IF,
IH(P)
, WB
detects endogenous levels of total 14-3-3 gamma protein
human, rat, mouse
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity
Reactivity
top
14-3-3γ
(poly)
IgG
Abcam
Cat# ab79165
immunogen = synthetic peptide from the N-terminal area of sheep 14-3-3 gamma
polyclonal rabbit
(liquid, whole antiserum, no preservative)
ELISA, WB
14-3-3 gamma protein
human, rat, mouse, sheep, cow, chicken
14-3-3γ
(mono)
IgG
1
, κ
AnaSpec
Cat# 53200
immunogen = recombinant 14.3.3-γ protein of human origin
monoclonal rabbit
(liquid, protein-G purified, PBS, BSA, azide)
IP, WB
14-3-3gamma
human
14-3-3γ
(HS23)
IgG
1
,κ
Novus Biologicals
Cat# NB100-407
immunogen = an N-terminal fragment of human 14-3-3 gamma
monoclonal mouse
(liquid, ascites)
WB
specific for human non-modified N-terminus 14-3-3 gamma; naturally occurring form of 14-3-3 gamma in which the N-terminus Met is removed and the Val is acetylated is not recognized by this antibody
human
(others not tested)
14-3-3γ
(KC21)
IgG
2a
Novus Biologicals
Cat# NB100-406
immunogen = an N-terminal fragment of human 14-3-3 gamma where the N-terminal Val was acetylated
monoclonal mouse
(liquid, ascites)
WB
specific for human 14-3-3 gamma; where the N-terminal Met is removed, resulting in an acetylated N-terminal Val. Unprocessed (non-modified) 14-3-3 gamma is not recognized by this antibody
human
(others not tested)
14-3-3γ (6A1)
(mono)
IgG
2a
Santa Cruz
Cat# sc-69955
Cat# sc-69955 P (peptide)
immunogen = raised against the N-terminus of human 14-3-3 γ
monoclonal mouse
(liquid, PBS, azide, gelatin)
IP, WB
14-3-3γ (gamma);
not
recommended for detection of unprocessed (non-modified) 14-3-3 γ
human
14-3-3γ (A-12)
(poly)
IgG
Santa Cruz
Cat# sc-31958
Cat# sc-31958 P (peptide)
immunogen = epitope mapping at within an internal region of human 14-3-3 γ
polyclonal goat
(liquid, PBS, azide, gelatin, affinity purified)
ELISA, IF, IP, WB
14-3-3γ and, to a lesser extent, 14-3-3ζ
human, rat, mouse
14-3-3γ (C-16)
(poly)
IgG
2a
Santa Cruz
Cat# sc-731
Cat# sc-731 P (peptide)
immunogen = epitope mapping at the C-terminus of human 14-3-3 γ
polyclonal rabbit
(liquid, PBS, azide, gelatin)
ELISA, IF,
IH(P)
, IP, WB
14-3-3γ and, to a lesser extent, 14-3-3 η
human, rat, mouse, cow, mammal (various)
14-3-3γ (KC21)
(mono)
IgG
2a
Santa Cruz
Cat# sc-59421
immunogen = raised against the N-terminus of human 14-3-3 γ
monoclonal mouse
(liquid, PBS, azide, gelatin)
IP, WB
detection of the naturally occurring form of 14-3-3 γ where the N-terminal Met is removed, resulting in an acetylated N-terminal Valine;
not
recommended for detection of unprocessed (non-modified) 14-3-3 γ
human
14-3-3γ (L-17)
(poly)
IgG
Santa Cruz
Cat# sc-31957
Cat# sc-31957 P (peptide)
immunogen = epitope mapping at within an internal region of human 14-3-3 γ
polyclonal goat
(liquid, PBS, azide, gelatin, affinity purified)
ELISA
14-3-3γ and, to a lesser extent, 14-3-3ζ
human, rat, mouse
14-3-3ε
(5A5)
IgG
2a
, κ
Abcam
Cat# ab71506
immunogen = recombinant full length human protein purified from
E. coli
monoclonal mouse
(liquid, PBS, azide, protein G purified)
ELISA, WB
14-3-3epsilon
human
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity
Reactivity
top
14-3-3ε
(poly)
IgG
Abcam
Cat# ab43057
immunogen = synthetic peptide from within N-terminus of 14-3-3 epsilon
polyclonal rabbit
(liquid, whole antiserum)
ELISA,
IH(P)
, WB
14-3-3epsilon; may
not
react with recombinant protein expressed in
E. coli
that is not N-acetylated
human, rat, mouse, rabbit, sheep, cow, chicken
(all mammals)
14-3-3ε
(poly)
IgG
Abcam
Cat# ab40117
immunogen = synthetic peptide from C-terminus of 14-3-3 epsilon
polyclonal rabbit
(liquid, whole antiserum)
ELISA,
IH(P)
, WB
14-3-3epsilon
human, rat, mouse, rabbit, sheep, cow, chicken
(all mammals)
14-3-3ε
(poly)
IgG
Abcam
Cat# ab64983
immunogen = synthetic peptide from C-terminus of human 14-3-3 epsilon
polyclonal rabbit
(liquid, PBS, glycerol, NaCl, azide, affinity purified)
ELISA, WB
detects endogenous levels of total 14-3-3 epsilon protein
human, rat, mouse
14-3-3ε
(poly)
IgY
Abcam
Cat# ab40117
immunogen = full length protein human 14-3-3 epsilon
polyclonal chicken
(liquid, PBS, no preservative, affinity purified)
WB
14-3-3epsilon
human, rat, mouse, cow
14-3-3ε
(poly)
IgG
LifeSpan Biosciences, Inc.
Cat# LS-C42371
immunogen = synthetic corresp. to C-terminal sequence of sheep 14-3-3ε
polyclonal rabbit
(liquid, serum)
ELISA,
IH(P)
, WB
14-3-3ε (C-terminal, 30kDa)
human, rat, mouse, rabbit, sheep, bovine, chicken
14-3-3ε
(poly)
IgG
LifeSpan Biosciences, Inc.
Cat# LS-C42374
immunogen = synthetic peptide corresp. to acetylated N-terminal sequence of sheep 14-3-3ε
polyclonal rabbit
(liquid, serum)
ELISA,
IH(P)
, WB
14-3-3ε (N-terminal, 30kDa)
human, rat, mouse, rabbit, sheep, bovine, chicken
14-3-3ε
(poly)
IgG
LifeSpan Biosciences, Inc.
Cat# LS-C40063
immunogen = human YWHAE (aa1-256) with GST Tag
polyclonal mouse
(liquid, PBS, unpurified serum)
ELISA, WB
14-3-3 protein epsilon (YWHAE)
human
14-3-3ε
(poly)
IgY
LifeSpan Biosciences, Inc.
Cat# LS-C32298
Discontinued
immunogen = recombinant protein from human YWHAE (aa1-255)
polyclonal chicken
(liquid, PBS, no preservative, affinity purfied)
WB
14-3-3 protein epsilon (YWHAE)
human, rat, mouse, bovine
14-3-3ε
(8j12)
IgG
2a
, κ
US Biological
Cat# 0014-04
immunogen = recombinant human 14-3-3 epsilon (1-255aa) purified from
E. coli
monoclonal mouse
(liquid, PBS, azide, protein G affinity purified)
ELISA, WB
wide range of peptides containing this phosphorylated 14-3-3 binding motif;
no
cross-reaction with corresponding non-phosphorylated sequences
human
14-3-3ε (5A5)
(mono)
IgG
2a
Santa Cruz
Cat# sc-130547
immunogen = full length recombinant human 14-3-3ε
monoclonal mouse
(liquid, PBS, azide, gelatin)
ELISA, WB
14-3-3ε (epsilon)
human, mouse
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity
Reactivity
top
14-3-3ε (8C3)
(mono)
IgG
2a
Santa Cruz
Cat# sc-23957
immunogen = partially purified proteins of sheep pineal gland
monoclonal mouse
(liquid, PBS, azide, gelatin)
IF,
IH(P)
, IP, WB
14-3-3ε (epsilon)
human, rat
14-3-3ε (A-14)
(poly)
IgG
Santa Cruz
Cat# sc-31961
Cat# sc-31961 P (peptide)
immunogen = epitope mapping within an internal region of human 14-3-3 ε
polyclonal goat
(liquid, PBS, azide, gelatin)
ELISA, IF, WB
14-3-3ε (epsilon)
human, rat, mouse, cow, mammal (various)
14-3-3ε (E-20)
(poly)
IgG
Santa Cruz
Cat# sc-31962
Cat# sc-31962 P (peptide)
immunogen = epitope mapping within an internal region of human 14-3-3 ε
polyclonal goat
(liquid, PBS, azide, gelatin, affinity purified)
ELISA, IF, IP, WB
14-3-3ε (epsilon)
human, rat, mouse, cow, mammal (various)
14-3-3ε (T-16)
(poly)
IgG
Santa Cruz
Cat# sc-1020
Cat# sc-1020 P (peptide)
immunogen = epitope mapping within a divergent domain of human 14-3-3 ε
polyclonal rabbit
(liquid, PBS, azide, gelatin, affinity purified)
ELISA, IF, IP, WB
14-3-3ε (epsilon)
human, rat, mouse, sheep, cow, mammal (various)
14-3-3ζ
(8C3)
IgG
2a
Abcam
Cat# ab36777
immunogen = tissue / cell preparation (sheep pineal gland)
monoclonal mouse
(liquid, ascites)
WB
14-3-3 zeta
rat, sheep
14-3-3ζ
(poly)
IgG
Abcam
Cat# ab51129
immunogen = synthesized non-phosphopeptide derived from human 14-3-3 zeta around the phosphorylation site of serine 58 (R-S-Sp-W-R)
polyclonal rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified)
ELISA,
IH(P)
, WB
endogenous levels of total 14-3-3 zeta protein
human, rat, mouse
14-3-3ζ
(poly)
IgG
Abcam
Cat# ab32622
immunogen = synthetic peptide: A G D D K K G I V D Q S Q Q A Y, corresp. to aa134 - 149 of human 14-3-3 zeta
polyclonal rabbit
(liquid, PBS, BSA, azide, affinity purified)
IH(P)
, WB
specific for 14-3-3 zeta (30kDa)
human, rat, mouse
(expect chicken, cow and dog due to seq. homology)
14-3-3ζ
(poly)
IgG
Abcam
Cat# ab69593
immunogen = synthetic peptide from an internal region of human 14-3-3 zeta
polyclonal rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified)
ELISA, WB
specific for 14-3-3 zeta (30kDa)
human, rat, mouse
14-3-3ζ
(poly)
IgG
Abcam
Cat# ab52875
immunogen = recombinant fusion protein corresp. to aa51-150 of mouse 14-3-3 zeta
polyclonal mouse
(liquid, whole serum, glycerol, no preservative)
WB
specific for 14-3-3 zeta (30kDa)
mouse
(human, rat, chicken, cow, sheep due to seq. homology)
14-3-3ζ
(poly)
IgG
Abcam
Cat# ab32623
immunogen = synthetic peptide: A G D D K K G I V D Q S Q Q A Y, corresp. to aa134 - 149 of human 14-3-3 zeta
polyclonal rabbit
(liquid, PBS, BSA, azide, affinity purified, prediluted)
IH(P)
specific for 14-3-3 zeta
human, rat, mouse
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity
Reactivity
top
14-3-3ζ
(poly)
AnaSpec
Cat# 53204
immunogen = a synthetic peptide corresponding to a protein sequence close to amino acids 134-149 of human 14.3.3-ζ
polyclonal rabbit
(epitope specific, affinity purified, PBS, BSA, azide)
IH(P)
, WB
recognizes 14-3-3zeta
human, rat, mouse
14-3-3ζ
(poly)
IgG
Biolegend
Cat# 637801/2
immunogen = full-length human 14-3-3 zeta protein
polyclonal rabbit
(liquid, PBS, glycerol, azide, affinity purified)
WB
28 kDa 14-3-3ζ
human
(mouse, very weak)
14-3-3ζ (C16)
(poly)
IgG
Santa Cruz
Cat# sc-1019
Cat# sc-1019 P (peptide)
immunogen = epitope mapping within a relatively divergent domain of human 14-3-3ζ
polyclonal rabbit
(liquid, PBS, azide, gelatin, affinity purified)
ELISA, IF,
IH(P)
, IP, WB
14-3-3ζ and, to a lesser extent, 14-3-3 β and σ
human, rat, mouse, sheep, cow, mammal (various)
14-3-3ζ (L-15)
(poly)
IgG
Santa Cruz
Cat# sc-31964
Cat# sc-31964 P (peptide)
immunogen = epitope mapping at within an internal region of human 14-3-3ζ
polyclonal goat
(liquid, PBS, azide, gelatin, affinity purified)
ELISA
14-3-3ζ ; and, to a lesser extent, 14-3-3α and β
human, rat, mouse
14-3-3ζ (V-16)
(poly)
IgG
Santa Cruz
Cat# sc-31965
Cat# sc-31965 P (peptide)
immunogen = epitope mapping within an internal region of human 14-3-3ζ
polyclonal goat
(liquid, PBS, azide, gelatin, affinity purified)
ELISA, IF, IP, WB
14-3-3ζ and, to a lesser extent, 14-3-3 ε
human, rat, mouse
14-3-3ζ phos Ser58
(poly)
IgG
Abcam
Cat# ab51109
immunogen = synthetic phosphopeptide from human 14-3-3-zeta, around the phosphorylation site of serine 58 (R-S-pS-W-R)
polyclonal rabbit
(liquid, PBS, azide, NaCl, glycerol, affinity purified)
ELISA,
IH(P)
, WB
endogenous levels of 14-3-3 zeta only when phosphorylated at serine 58 (28kDa)
human, rat, mouse
14-3-3ζ phos Ser58
(poly)
IgG
Santa Cruz
Cat# sc-101623
immunogen = epitope corresp. to phosphorylated Ser 58 of human 14-3-3ζ
polyclonal rabbit
(liquid, PBS, azide, gelatin)
IF,
IH(P)
, IP, WB
detection of Ser 58 phosphorylated 14-3-3 ζ
human, rat, mouse
14-3-3ζ/δ
(poly)
IgG
Abcam
Cat# ab63635
immunogen = synthetic non-phosphopeptide derived from human 14-3-3 zeta + delta around the phosphorylation site of threonine 232 (S-D-TP-Q-G)
polyclonal rabbit
(liquid, PBS, glycerol, NaCl, azide, affinity purified)
ELISA, IC, IF,
IH(P)
, WB
detects endogenous levels of total 14-3-3 zeta + delta protein.
human, mouse
14-3-3ζ/δ
(poly)
IgG
Abcam
Cat# ab37560
immunogen = synthetic peptide selected within aa 200-300 of human 14-3-3 zeta (C-terminal)
polyclonal rabbit
(liquid, PBS, azide, protein G purified)
ELISA,
IH(P)
, WB
specific for 14-3-3 zeta + delta
human
14-3-3ζ/δ
(poly)
IgG
Abcam
Cat# ab18706 (clone P6148)
immunogen = recombinant frag., corresp. to N-terminal aa1-150 of 14-3-3 zeta
polyclonal rabbit
(liquid, PBS, glycerol, azide, affinity purified)
WB
specific for 14-3-3 zeta + delta
human, mouse
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity
Reactivity
top
14-3-3ζ/δ
(poly)
IgG
Biolegend
Cat# 614801/2
immunogen = NIH-recombinant (partial), N-terminal 14-3-3 protein
polyclonal rabbit
(liquid, PBS, glycerol, azide, affinity purified)
WB
30 kDa 14-3-3ζ/δ
human, mouse
14-3-3ζ/δ phos T232
(poly)
IgG
Abcam
Cat# ab59374
immunogen = synthetic phosphopeptide derived from human 14-3-3 zeta + delta around the phosphorylation site of threonine 232 (S-D-Tp-Q-G)
polyclonal rabbit
(liquid, PBS, NaCl, azide, glycerol, affinity purified)
ELISA,
IH(P)
detects endogenous levels of 14-3-3 zeta + delta only when phosphorylated at threonine 232
human, mouse
14-3-3η
(poly)
IgG
Abcam
Cat# ab28665
immunogen = synthetic peptide, corresp. to a region between aa71-120 of human 14-3-3η
polyclonal rabbit
(liquid, PBS, no preservative, sucrose, affinity purified)
IH(P)
, WB
14-3-3eta (28kDa)
human
14-3-3η
(poly)
IgG
Abcam
Cat# ab69591
immunogen = synthetic peptide (human) from an internal region
polyclonal rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified)
ELISA, IC, IF, WB
detects endogenous levels of total 14-3-3eta
human, rat, mouse
14-3-3η
(poly)
IgG
2a
, κ
Abcam
Cat# ab58073
immunogen = recombinant fragment corresp. to aa71-170 of human 14-3-3 eta
polyclonal rabbit
(liquid, PBS, no preservative, protein G purified)
WB
14-3-3eta
human
14-3-3η
(poly)
IgG
Abcam
Cat# ab79163
immunogen = synthetic peptide from the N-terminal area of sheep 14-3-3 eta
polyclonal rabbit
(liquid, whole serum, no preservative)
WB
14-3-3eta
human, rat, mouse, rabbit, sheep, cow, chicken
14-3-3η
(poly)
IgG
LifeSpan Biosciences, Inc.
Cat# LS-C29907
immunogen = synthetic peptide corresp. to a region of human 14-3-3η (YWHAH)
polyclonal rabbit
(lyophilized, affinity purified)
IH, WB
14-3-3 protein eta(YWHAH)
human, rat, mouse
14-3-3η
(poly)
IgG
LifeSpan Biosciences, Inc.
Cat# LS-C42372
immunogen = synthetic peptide corresp. to acetylated N-terminal sequence of sheep 14-3-3 eta
polyclonal rabbit
(liquid, serum)
IH(P)
, WB
14-3-3 protein eta(YWHAH)
human, rat, mouse, rabbit, sheep, bovine, chicken
14-3-3η (K-12)
(poly)
IgG
Santa Cruz
Cat# sc-17286
Cat# sc-17286 P (peptide)
immunogen = epitope mapping within an internal region of human 14-3-3 η
polyclonal goat
(liquid, PBS, azide, gelatin)
ELISA, IF, IP, WB
14-3-3η (zeta)
human, rat, mouse
14-3-3η/γ/ζ
(poly)
IgG
Abcam
Cat# ab14124
immunogen = Synthetic peptide: K M K G D Y Y R Y L A E, corresp. to aa124-135 of 14-3-3 eta + gamma + zeta
polyclonal rabbit
(liquid, whole serum, no preservative)
IH(P)
, WB;
not
IP
recognizes eta, gamma and zeta isoforms at 27kDa
mammal (various)
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