|
Antibodies to AGE and RAGE Proteins
|
| From the Manufacturers
|
Antigen (Clone) IgG |
Distributor (Producer) |
Immunogen (Epitope) |
Host (Formulation) |
Methods glossary |
Specificity |
Reactivity top |
AGE (poly) IgG
|
Abcam
cat # ab23720 |
immunogen = Advanced Glycation End Products- a mixture of BSA-AGE and HSA-AGE |
polyclonal rabbit (liquid, whole antiserum, azide) |
ELISA |
different AGE-products such as CML, Imidazolone and others |
human, various species (others not tested) |
AGE (poly) IgG
|
Abcam
cat # ab23722 |
immunogen = Advanced Glycation End Products- a mixture of BSA-AGE and HSA-AGE |
polyclonal rabbit (liquid, PBS, azide, affinity purified) |
ELISA |
AGE-products; cross-reacts with BSA-AGE; HSA-AGE <1% |
human (others not tested) |
AGE (poly) IgG
|
Autogen Bioclear
cat# GTX43720 produced by GeneTex |
immunogen = advanced glycation end products - a mixture of BSA-AGE and HSA-AGE |
polyclonal rabbit (liquid, antiserum, azide) |
ELISA |
reacts with a number of different AGE-products such as CML, Imidazolone and others |
human, various species |
AGE (poly)
|
Chemicon and Upstate are Millipore
cat# AB9890 |
immunogen = glycoaldehyde-modified protein |
polyclonal goat (liquid, serum, thimerosal) |
ELISA, WB |
AGE (Advanced Glycated End-products) |
human (others not tested) |
AGE (poly)
|
RDI /Fitzgerald
cat# RDI-GPA036H new Cat# = 20R-AG003 |
immunogen = glycoaldehyde-modified protein |
polyclonal goat (liquid, neat serum, no preservative) |
ELISA, WB |
advanced glycation end-product epitopes |
|
AGE (poly)
|
AbD Serotec
Cat# AHP1250 |
immunogen = glycated modified protein |
polyclonal goat (liquid, serum) |
ELISA, WB |
recognizes a variety of advanced glycated end product (AGE) epitopes |
human (others not tested) |
AGE (poly)
|
US Biological
cat# A0909-75C |
immunogen = carboxymethylated Keyhole Limpet Hemocyanin (CML-KLH). |
polyclonal goat (liquid, neat serum, azide) |
ELISA, WB |
binds to an N3-carboxymethyl-lysine (CML) modified proteins |
human |
AGE (poly) IgG
|
US Biological
cat# A0909-75D |
immunogen = carboxymethylated Keyhole Limpet Hemocyanin (CML-KLH). |
polyclonal goat (liquid, PBS, NaCl, azide, EDTA, affinity purified) |
ELISA, WB |
binds to an N3-carboxymethyl-lysine (CML) modified proteins |
|
AGE (poly) IgG
|
US Biological
cat# A0909-75E |
immunogen = carboxymethyllysine conj. to KLH (CML-KLH) |
polyclonal rabbit (liquid, PBS, azide, EDTA, affinity purified) |
ELISA, WB |
AGE |
|
AGE (cC-19) (poly) IgG
|
Santa Cruz
Cat# sc-9234 Cat# sc-9234 P (peptide) |
immunogen = peptide mapping at the C-terminus of AGE-1 of C. elegans origin |
polyclonal goat (liquid, affinity purified, PBS, azide) |
IF, WB |
AGE-1 |
C. elegans |
Antigen (Clone) IgG |
Distributor (Producer) |
Immunogen (Epitope) |
Host (Formulation) |
Methods glossary |
Specificity |
Reactivity top |
AGE-1 (cN-17) (poly) IgG
|
Santa Cruz
Cat# sc-9233 Cat# sc-9233 P (peptide) |
immunogen = peptide mapping near the N-terminus of AGE-1 of C. elegans origin |
polyclonal goat (liquid, affinity purified, PBS, azide) |
IF, WB |
AGE-1 |
C. elegans |
AGER (1C1) IgG2a, κ
|
Novus Biologicals
cat# H00000177-M02J |
immunogen = AGER full length recombinant protein with GST tag |
monoclonal mouse (liquid, PBS, purified, no preservative) |
ELISA, WB |
AGER - advanced glycosylation end product-specific receptor |
human (others not tested) |
AGER (1C1) IgG2a, κ
|
Novus Biologicals
cat# H00000177-M02A |
immunogen = AGER (AAH20669, 23-405 aa) full length recombinant protein with GST tag |
monoclonal mouse (liquid, ascites, purified, no preservative) |
ELISA, WB |
AGER - advanced glycosylation end product-specific receptor |
human (others not tested) |
AGER (1D1) IgG2a, κ
|
Novus Biologicals
cat# H00000177-M05 |
immunogen = AGER (AAH20669, aa87-197) partial recombinant protein with GST tag |
monoclonal mouse (liquid, ascites, PBS, purified, no preservative) |
ELISA, WB |
AGER - advanced glycosylation end product-specific receptor |
human (others not tested) |
AGER (2C2) IgG2a, κ
|
Novus Biologicals
cat# H00000177-M01 |
immunogen = AGER (AAH20669, aa23-405) full length recombinant protein with GST tag |
monoclonal mouse (liquid, ascites, PBS, purified, no preservative) |
ELISA, WB |
AGER - advanced glycosylation end product-specific receptor |
human (others not tested) |
AGER (poly)
|
Novus Biologicals
cat# H00000177-B01 |
immunogen = AGER (NP_001127, 1-404 aa) full length recombinant protein with GST tag |
polyclonal mouse (liquid, glycerol, purified, no preservative) |
ELISA, WB |
AGER - advanced glycosylation end product-specific receptor |
human (others not tested) |
AGER (poly)
|
Novus Biologicals
cat# H00000177-D01P |
immunogen = AGER (NP_001127.1, aa1-404) full-length human protein |
polyclonal rabbit (liquid, glycerol, purified, no preservative) |
ELISA, WB |
AGER - advanced glycosylation end product-specific receptor |
human (others not tested) |
AGER (2C2) IgG2a, κ
|
Sigma Aldrich
cat # WH0000177M1 |
immunogen = AGER (AAH20669, aa 23-405) full length recombinant protein with GST tag. MW of the GST tag alone is 26 kDa |
monoclonal mouse (liquid, PBS, sucrose, purified) |
ELISA, IB |
RAGE |
human |
AGER (poly)
|
Sigma Aldrich
cat # AV41642 |
immunogen = synthetic peptide corresp. to a region of human AGER with an internal ID of S09910 |
polyclonal rabbit (lyophilized, PBS, sucrose, affinity purified) |
IH, WB |
RAGE |
rat, mouse |
AGER (poly) IgG
|
Sigma Aldrich
cat # AV41643 |
immunogen = synthetic peptide corresp. to a region of human AGER with an internal ID of S09911 |
polyclonal rabbit (lyophilized, PBS, sucrose, affinity purified) |
IH, WB |
RAGE |
human |
Antigen (Clone) IgG |
Distributor (Producer) |
Immunogen (Epitope) |
Host (Formulation) |
Methods glossary |
Specificity |
Reactivity top |
EN-RAGE (poly) IgG
|
R&D Systems
Cat #AF1052 |
immunogen = purified E.coli-derived recombinant human extracellular newly identified RAGE-binding protein (EN-RAGE) |
polyclonal goat (affinity purified, PBS, filtered, lyophilized) |
ELISA, FC, IH, WB |
rhEN-RAGE |
human |
EN-RAGE (poly) IgG
|
R&D Systems
Cat# BAF1052 |
immunogen = purified E. coli-derived rh human extracellular newly identified RAGE-binding protein (EN-RAGE) |
polyclonal goat (lyophilized, PBS, BSA, filtered, affinity purified, biotinylated) |
FC, IH, WB |
EN-RAGE |
human |
EN-RAGE (poly) IgG
|
Sigma Aldrich
cat # E1032 |
immunogen = purified, E. coli-derived recombinant human extracellular RAGE-binding protein |
polyclonal goat (lyophilized, PBS, trehalose, filtered) |
ELISA, FC, IB, IH |
EN-RAGE |
human |
RAGE (mono) IgG2a, κ
|
Abcam
cat # ab54741 |
immunogen = recombinant full length protein, corresp. to aa23-405 of human RAGE
|
monoclonal mouse (liquid, PBS, no preservative, protein G purified) |
FC, WB |
RAGE |
human (others not tested) |
RAGE (poly) IgG
|
Abcam
cat # ab3611 Reference: Takeda, S, 2010 |
immunogen = synthetic peptide, (C)W R K R Q P(R/L) E E R K A P E S Q E D (N), corresp. to aa 362-380 of rat RAGE |
polyclonal rabbit (liquid, affinity purified, PBS, BSA, azide) |
FC, IH(F), WB |
RAGE |
rat, mouse (others not tested) |
RAGE (poly) IgG
|
Abcam
cat # ab-7764 |
immunogen = synthetic peptide corresp.to aa. 42-59 of human RAGE |
polyclonal goat (liquid, antisera, azide) |
IH(P) |
RAGE |
human (others not tested) |
RAGE (poly) IgG
|
Abcam
cat # ab20558 |
immunogen = synthetic peptide R A E L N Q S E E P E A G E S, corresp. to C-terminal amino acids 385-399 of human RAGE |
polyclonal goat (liquid, whole antisera) |
ELISA, IH(P) |
recognises amino acid sequence 385-399 of human receptor for advanced glycosolated end products (RAGE) |
human (others not tested) |
RAGE (poly) IgG
|
Abcam
cat # ab30381 |
immunogen = synthetic peptide, conj. to KLH, derived from within residues 350 to the C-terminus of Human RAGE |
polyclonal rabbit (liquid, affinity purified, PBS, BSA, azide) |
WB |
RAGE |
mouse (others not tested; expect Human) |
RAGE (poly) IgG
|
Abcam
cat # ab37647 |
immunogen = synthetic peptide, K G A P K K P P Q R L E W K L N T G R T C, corresp. to aa. 39-58 of human RAGE |
polyclonal rabbit (liquid, affinity purified, PBS, azide) |
ELISA, FC, WB |
RAGE |
human, mouse, cow (others not tested) |
RAGE (poly) IgG
|
Abcam
cat # ab-37647 |
Immunogen = synthetic peptide: K G A P K K P P Q R L E W K L N T G R T C, corresp. to aa39-58 of human RAGE |
polyclonal rabbit (liquid, PBS, azide, affinity purified) |
ELISA, FC, IC, IF, WB |
RAGE |
human, rat, mouse, cow (others not tested) |
Antigen (Clone) IgG |
Distributor (Producer) |
Immunogen (Epitope) |
Host (Formulation) |
Methods glossary |
Specificity |
Reactivity top |
RAGE (poly)
|
A B R - Thermo Scientific
cat # PA1-075 |
immunogen = synthetic peptide, C(362)W R K R Q P R(R/L) E E R K A P E S Q E D (N) (380) rat RAGE |
polyclonal rabbit (liquid, affinity purified, PBS, BSA, azide) |
IH(F), WB |
RAGE |
rat, mouse |
RAGE (poly)
|
A B R - Thermo Scientific
cat # PA1-23061 |
immunogen = synthetic peptide R A E L N Q S E E P E A G E S, corresp. to C-terminal amino acids 385-399 of human RAGE |
polyclonal goat (liquid, whole antisera) |
ELISA, IH(P) |
RAGE |
human |
RAGE (poly) IgG
|
A B R - Thermo Scientific
cat# PA1-38535 |
immunogen = synthetic peptide corresp. to residues 38-57 of human RAGE |
polyclonal rabbit (liquid, epitope affinity purified, PBS, BSA, azide) |
WB |
detects RAGE |
human, rat, mouse |
RAGE (poly)
|
A B R - Thermo Scientific
cat # PA1-26918 |
immunogen = synthetic peptide corresp. to residues 42-59 of human RAGE |
polyclonal goat (liquid, whole antisera) |
ELISA, IH(P) |
RAGE |
human |
RAGE (poly)
|
A B R - Thermo Scientific
cat # PA1-84173 |
immunogen = synthetic peptide corresp. to aa42-59 of human RAGE |
polyclonal goat (liquid, serum, azide) |
ELISA, IH(P) |
RAGE |
human |
RAGE (poly) IgG
|
AnaSpec
cat # 29618 |
immunogen = synthetic peptide corresp. to aa 38-57 of human RAGE |
polyclonal rabbit (liquid, affinity purified, PBS) |
ELISA, IP, WB |
RAGE |
human, mouse (may react to bovine and rat due to seq. homology) |
RAGE (mono) IgG2a
|
Autogen Bioclear
cat# LS-C26227 produced by LifeSpan Biosciences |
immunogen = recombinant truncated extracellular portion of human RAGE expressed in E. coli |
monoclonal mouse (liquid, serum, PBS, glycerol, thimerosal) |
ELISA, IF, IH(F, P), WB |
natural and recombinant RAGE |
human, bovine |
RAGE (8B42) IgG2a
|
Autogen Bioclear
cat# LS-C75327 produced by LifeSpan Biosciences |
immunogen = NS0-derived recombinant mouse RAGE extracellular domain |
monoclonal mouse (lyophilized, PBS, glycerol, trehalose) |
ELISA |
RAGE |
mouse |
RAGE (poly) IgG
|
Autogen Bioclear
cat# LS-C26228 produced by LifeSpan Biosciences |
immunogen = synthetic peptide corresponding to human receptor for advanced glycated end products, aa42-59, P K K P P Q R L E W K L N T G R T E |
polyclonal goat (liquid, serum, PBS, glycerol, thimerosal) |
ELISA, IH(F, P) |
RAGE |
human, rat, mouse |
RAGE (poly) IgG
|
Autogen Bioclear
cat# LS-C26226 produced by LifeSpan Biosciences |
immunogen = synthetic peptide corresp. to human receptor for advanced glycated end products, aa42-59, P K K P P Q R L E W K L N T G R T E |
polyclonal goat (liquid, serum, PBS, glycerol, thimerosal) |
ELISA, IH(F, P) |
recognizes aa 42-59 of human receptor for advanced glycated end products (RAGE) |
human |
Antigen (Clone) IgG |
Distributor (Producer) |
Immunogen (Epitope) |
Host (Formulation) |
Methods glossary |
Specificity |
Reactivity top |
RAGE (poly) IgG
|
Autogen Bioclear
cat# LS-C40337 produced by LifeSpan Biosciences |
immunogen = synthetic peptide corresp. to a region of human AGER |
polyclonal rabbit (liquid, serum, PBS, glycerol, thimerosal) |
IH(F), WB |
RAGE |
human |
RAGE (poly) IgG
|
Autogen Bioclear
cat# LS-C31853 produced by LifeSpan Biosciences |
immunogen = synthetic peptide corresp. to a region of human AGER |
polyclonal rabbit (liquid, PBS, azide, purified) |
IH(F), WB |
RAGE |
human |
RAGE (poly) IgG
|
Autogen Bioclear
cat# GTX40558 produced by GeneTex |
immunogen = synthetic peptide: R A E L N Q S E E P E A G E S, corresp. to C- terminal aa385-399 of human RAGE |
polyclonal goat (liquid, unpurified antiserum, azide) |
ELISA, IH(P) |
recognises aa seq. 385-399 of human receptor for advanced glycosolated end products (RAGE) |
human |
RAGE (poly) IgG
|
Autogen Bioclear
cat# GTX27764 produced by GeneTex |
immunogen = synthetic peptide - aa42-59 of human RAGE |
polyclonal goat (liquid, unpurified antiserum, azide) |
ELISA, IH(P) |
RAGE |
human |
RAGE (poly) IgG
|
Autogen Bioclear
cat# GTX23611 produced by GeneTex |
immunogen = synthetic peptide: C(362)W R K R Q P R (R/L)E E R K A P E S Q E D (NH2)(380) corresp. to aa362 - 380 from rat RAGE |
polyclonal rabbit (liquid, PBS, BSA, azide, affinity purified) |
IH(F), WB |
RAGE |
human, rat, mouse |
RAGE (poly)
|
Biodesign Meridian LifeScience
cat# Q2B710G |
immunogen = synthetic peptide P K K P P Q R L E W K L N T G R T E corresp. to aa 42-59 of human RAGE |
polyclonal goat (liquid, neat, azide) |
ELISA, IH(P) |
RAGE |
human |
RAGE (poly)
|
Biodesign Meridian LifeScience
cat# Q2B300G |
immunogen = synthetic peptide R A E L N Q S E E P E A G E S corresp. to aa 385-399 of human RAGE |
polyclonal goat (liquid, neat, azide) |
ELISA, IH(P) |
RAGE |
human |
RAGE (poly) IgG
|
Calbiochem EMD Biosciences
cat # ST1025 |
immunogen = synthetic peptide (P K K P P Q R L E W K L N T G R T E) corresp.to amino acids 42-59 of human RAGE |
polyclonal goat (liquid, Neat serum) |
IH(P) |
RAGE |
human |
RAGE (DD/A11) IgG2a
|
Chemicon and Upstate are Millipore
cat# MAB5328 |
immunogen = recombinant truncated extracellular part of RAGE produced in E. coli |
monoclonal mouse (liquid, PBS, azide) |
ELISA, IC(C), IH(P), WB |
RAGE both natural and recombinant |
human, bovine (others not tested) |
RAGE (poly)
|
Chemicon and Upstate are Millipore
cat# AB5484 |
immunogen = synthetic peptide corr. to aa 42-59 of human RAGE |
polyclonal goat (liquid, serum, thimerosal; also available azide free [AB5484Z]) |
ELISA, IH(P), WB |
RAGE from diabetic tissues and vessels and neurons from AD patients |
human |
Antigen (Clone) IgG |
Distributor (Producer) |
Immunogen (Epitope) |
Host (Formulation) |
Methods glossary |
Specificity |
Reactivity top |
RAGE (poly)
|
Chemicon and Upstate are Millipore
cat# AB5601 |
immunogen = synthetic peptide from C-terminus of human RAGE |
polyclonal goat (liquid, serum, thimerosal) |
ELISA, IH(P) |
RAGE from diabetic tissues and vessels |
human (others not tested) |
RAGE (poly)
|
Chemicon and Upstate are Millipore
cat# AB5484Z (azide free) |
immunogen = synthetic peptide corr. to aa 42-59 of human RAGE |
polyclonal goat (liquid, serum, no preservative) |
ELISA, IH(P) |
RAGE from diabetic tissues and vessels and neurons from AD patients |
human |
RAGE (poly) IgG
|
Chemicon and Upstate are Millipore
cat# AB9714 |
immunogen = synthetic peptide from human RAGE |
polyclonal rabbit (liquid, PBS, azide, affinity purifird) |
ELISA, FC, WB |
RAGE |
human, mouse, bovine |
RAGE (poly)
|
GeneTex
cat # GTX104958 |
immunogen = protein antigen contains a seq. within a region between 151 and 380 of the human RAGE protein |
polyclonal rabbit (liquid, tris, clycine, glycerol, thimerosal, affinity purified) |
WB |
RAGE |
human |
RAGE (poly) IgG
|
GeneTex
cat # GTX23611 |
immunogen = synthetic peptide: C(362)W R K R Q P R (R/L) E E R K A P E S Q E D (NH2)(380) corresp. to aa residues 362 - 380 from rat RAGE |
polyclonal rabbit (liquid, PBS, BSA, azide, affinity purified) |
IH(F), WB |
RAGE |
rat, mouse (others species not tested) |
RAGE (poly) IgG
|
GeneTex
cat # GTX27764
produced by Abcam |
immunogen = synthetic peptide corresp.to aa42-59 of human RAGE |
polyclonal goat (liquid, serum, azide) |
ELISA, IH(P) |
RAGE |
human (other species not tested) |
RAGE (poly) IgG
|
GeneTex
cat # GTX40558
|
immunogen = synthetic peptide, R A E L N Q S E E P E A G E S, corresp. to C- terminal aa 385-399 of human RAGE. |
polyclonal goat (liquid, whole antiserum, azide) |
ELISA, IH(P) |
recognises aa seq. 385-399 of RAGE |
human (other species not tested) |
RAGE (E182) IgG2, κ
|
Novus Biologicals
cat# NBP1-19732 |
|
monoclonal rat (liquid, antiserum, PBS, affinity purified, no preservative) |
WB |
detects endogenous levels of RAGE protein |
human (others not tested) |
RAGE (RM017-2B65) IgG2, κ
|
Novus Biologicals
cat# NBP1-22504 |
immunogen = mouse recombinant protein of the extra cellular domain of RAGE protein |
monoclonal rat (liquid, ascites, PBS, protein G purified, no preservative) |
IH(F), WB |
RAGE |
mouse (others not tested) |
RAGE (poly) IgG
|
Novus Biologicals
cat # NB100-63063 |
immunogen = peptide corresp. to aa42-59 of human RAGE |
polyclonal goat (liquid, antisera, azide) |
ELISA, IH(P) |
RAGE |
human (others not tested) |
Antigen (Clone) IgG |
Distributor (Producer) |
Immunogen (Epitope) |
Host (Formulation) |
Methods glossary |
Specificity |
Reactivity top |
RAGE (poly) IgG
|
Novus Biologicals
cat # NB300-616 |
immunogen = synthetic peptide W R K R Q P R (R/L)E E R K A P E D Q E D corresp. to aa 362-380 of rat RAGE |
polyclonal rabbit (liquid, PBS, BSA, azide, affinity purified) |
IH, WB |
recognizes aa sequence 362-380 of human receptor for advanced glycosolated end products (RAGE) |
rat, mouse (others not tested) |
RAGE (poly) IgG
|
Novus Biologicals
cat # NB100-65691 |
immunogen = peptide corresp. to aa42-59 of human RAGE |
polyclonal goat (liquid, antisera, azide) |
ELISA, IH(P), WB |
RAGE |
human, rat, mouse (others not tested) |
RAGE (poly) IgG
|
Novus Biologicals
cat # NB120-7764 |
immunogen = synthetic peptide, corresp. to aa42-59 of human RAGE |
polyclonal goat (liquid, antisera, azide) |
ELISA, IH(P) |
RAGE |
human (others not tested) |
RAGE (175409) IgG2a
|
R&D Systems
Cat# MAB11792 |
immunogen = NS0-derived recombinant mouse RAGE, extracellular domain |
monoclonal rat (lyophilized, filtered, PBS, BSA) |
ELISA, WB |
RAGE |
rat, mouse |
RAGE (175410) IgG2a
|
R&D Systems
Cat# MAB1179 |
immunogen = purified NS0-derived recombinant mouse RAGE, extracellular domain |
monoclonal rat (lyophilized, supernate, filtered, PBS, trehalose, protein-G purified) |
ELISA, IH, WB |
RAGE; no cross-reactivity with rhRAGE |
rat, mouse |
RAGE (175440) IgG2a
|
R&D Systems
Cat# BAM11791 |
immunogen = NS0-derived recombinant mouse RAGE, extracellular domain |
monoclonal rat (lyophilized, filtered, PBS, BSA, biotinylated) |
ELISA, WB |
RAGE extracellular domain |
rat, mouse |
RAGE (176902) IgG2b
|
R&D Systems
Cat# MAB11451 |
immunogen = purified NS0-derived recombinant human RAGE, extracellular domain |
monoclonal mouse (lyophilized, filtered, PBS, trehalose) |
Block, ELISA, WB |
RAGE; in WB 25% cross-reactivity with rmRAGE and rrRAGE |
human |
RAGE (176907) IgG1
|
R&D Systems
Cat# MAB1145 |
immunogen = purified NS0-derived recombinant human RAGE, extracellular domain |
monoclonal mouse (supernate, protein-G, PBS, trehalose, filtered, lyophilized) |
ELISA, WB |
RAGE |
human (no cross with rat or mouse) |
RAGE (poly) IgG
|
R&D Systems
Cat# AF1145 |
immunogen = purified NS0-derived recombinant human RAGE, extracellular domain |
polyclonal goat (affinity purified, PBS, trehalose, filtered, lyophilized) |
Block, ELISA, IH, WB |
rhRAGE |
human |
RAGE (poly) IgG
|
R&D Systems
Cat# AF1179 |
immunogen = purified NS0-derived recombinant mouse RAGE, extracellular domain |
polyclonal goat (affinity purified, PBS, trehalose, filtered, lyophilized) |
Block, ELISA, IH, WB |
RAGE; in ELISA, 25% crossd with rrRAGE and 5% cross with rhRAGE |
mouse |
Antigen (Clone) IgG |
Distributor (Producer) |
Immunogen (Epitope) |
Host (Formulation) |
Methods glossary |
Specificity |
Reactivity top |
RAGE (poly) IgG
|
R&D Systems
Cat# BAF1145 |
immunogen = purified NS0-derived recombinant human RAGE, extracellular domain |
polyclonal goat (affinity purified, Biotin, PBS, BSA, filtered, lyophilized) |
ELISA, WB |
rhRAGE; in WB, 25% cross with rmRAGE and rrRAGE |
human |
RAGE (poly) IgG
|
R&D Systems
Cat# BAF1179 |
immunogen = purified NS0-derived recombinant mouse RAGE, extracellular domain |
polyclonal goat (affinity purified, Biotin, PBS, BSA, filtered, lyophilized) |
WB |
RAGE; in WB, 30% cross with rrRAGE and 10% cross with rhRAGE |
mouse |
RAGE (poly) IgG
|
R&D Systems
Cat# AF1616 |
immunogen = purified NS0-derived recombinant rat RAGE, extracellular domain |
polyclonal goat (lyophilized, filtered, PBS, trehalose, affinity purified) |
ELISA, WB |
RAGE extracellular domain |
rat |
RAGE (poly) IgG
|
R&D Systems
Cat# BAF4750 |
immunogen = purified, NS0-derived, recombinant canine RAGE extracellular domain |
polyclonal sheep (lyophilized, PBS, BSA, filtered, affinity purified, biotinylated) |
ELISA, WB |
RAGE |
dog |
RAGE (poly) IgG
|
R&D Systems
Cat# AF4750 |
immunogen = purified, NS0-derived, recombinant canine RAGE extracellular domain |
polyclonal sheep (lyophilized, PBS, BSA, filtered, affinity purified) |
ELISA, WB |
RAGE extracellular domain |
dog |
RAGE (kit)
|
R&D Systems
Cat# DRG00 |
|
(capture ab = monoclonal anti-RAGE; detection ab = polyclonal anti-RAGE) |
ELISA |
quantitative determination of extracellular domain of RAGE |
human (cell culture supernates, serum and plasma) |
RAGE (kit)
|
R&D Systems
Cat# DY1145 |
|
(capture ab = monoclonal anti-hRAGE; detection ab = polyclonal goat anti-hRAGE biotinylated) |
ELISA |
quantitative determination of natural and recombinant RAGE |
human (cell culture supernates, serum and plasma) |
RAGE (kit)
|
R&D Systems
Cat# DY1179 |
|
(capture ab = rat monoclonal anti-mRAGE; detection ab = polyclonal rat anti-mRAGE biotinylated) |
ELISA |
quantitative determination of natural and recombinant RAGE |
human (cell culture supernates, serum and plasma) |
RAGE (poly)
|
RDI /Fitzgerald
Cat# RAGEabG New Cat# 20R-RG002 |
immunogen = synthetic peptide corr. to aa 42-59 (P K K P P Q R L E W K L N T G R T E) of human RAGE |
polyclonal goat (liquid, neat serum, azide or azide-free, positivecontrol) |
ELISA, IH(P) |
RAGE |
human |
RAGE (poly)
|
RDI /Fitzgerald
Cat# RAGECabG New Cat# = 20R-RG001 |
immunogen = synthetic peptide corresp. to aa 385-399 (R A E L N Q S E E P E A G E S) of human RAGE |
polyclonal goat (liquid, whole serum, azide or azide-free, positivecontrol) |
ELISA, IH(P) |
RAGE from vessels from diabetic tissues and vessels and neurons from AD brains |
human |
Antigen (Clone) IgG |
Distributor (Producer) |
Immunogen (Epitope) |
Host (Formulation) |
Methods glossary |
Specificity |
Reactivity top |
RAGE (poly)
|
RDI /Fitzgerald
cat# 20R-RR001 |
synthetic peptide corr. to aa 362-380 of RAGE |
polyclonal rabbit (liquid, affinity purified, PBS, BSA, azide) |
IH, WB |
detects C-terminal RAGE |
rat, mouse |
RAGE (poly)
|
AbD Serotec
Cat# AHP594 |
immunogen = peptide corresp. to aa 42-59 of human RAGE |
polyclonal goat (liquid, serum, azide) |
ELISA, IH(P) |
RAGE |
human (others not tested) |
RAGE (poly)
|
Sigma Aldrich
cat # R5278 |
immunogen = synthetic peptide corresp. to aa residues 362 - 380 from rat RAGE. |
polyclonal rabbit (liquid, PBS, BSA, azide, affinity purified) |
IH(F), WB |
RAGE |
rat, mouse |
RAGE (2Q96) IgG2a
|
US Biological
cat# R1031-15 |
immunogen = recombinant truncated extracellular part of RAGE produced in E. coli |
monoclonal mouse (liquid, PBS, azide, glycerol, protein A purified) |
ELISA, IC, IH, WB |
RAGE both natural and recombinant |
human, bovine |
RAGE (8A336) IgG
|
US Biological
cat# R1031-10C |
immunogen = monoclonal recombinant mouse RAGE |
monoclonal rat (liquid, serum, azide, glycerol, protein G purified) |
ELISA, IH, WB |
RAGE |
mouse (not human) |
RAGE (8B42) IgG2a
|
US Biological
cat# R1031-16 |
immunogen = NS0-derived recombinant mouse RAGE extracellular domain |
monoclonal rat (lyophilized, PBS, trehalose, protein G affinity purified) |
ELISA, WB |
RAGE |
mouse |
RAGE (poly) IgG
|
US Biological
cat# R1031-20 |
immunogen = synthetic peptide P K K P P Q R L E W K L N T G R T E that corresponds to aa 42-59 of the human receptor for advanced glycated end products |
polyclonal goat (liquid, serum, PBS, thimerosal, glycerol) |
ELISA, IH |
specific for Receptor for Advanced Glycosylation End products (RAGE) |
human |
RAGE (poly) IgG
|
US Biological
cat# R1031-10 |
immunogen = synthetic peptide P K K P P Q R L E W K L N T G R T E that corresp. to aa 42-59 of the human receptor for advanced glycated end products (RAGE) |
polyclonal goat (liquid, serum, azide, glycerol) |
ELISA, IH |
specific aa seq. 42-59 of human receptor for advanced glycated end products |
human |
RAGE (poly) IgG
|
US Biological
cat# R1031-20B |
immunogen = synthetic peptide corresp. to aa 42-59 of the human receptor for advanced glycated end products |
polyclonal goat (liquid, serum, PBS, thimerosal, glycerol) |
ELISA, IH(P) |
specific for Receptor for Advanced Glycosylation End products (RAGE) |
human |
RAGE (poly) IgG
|
US Biological
cat# R1031-20C |
immunogen = synthetic peptide: C(362)W R K R Q P R (R/L) E E R K A P E S Q E D (NH2)(380). Immunizing peptide corresponds to aa residues 362 - 380 from rat RAGE |
polyclonal rabbit (liquid, PBS, BSA, azide, affinity purified) |
IH(F), WB |
specific for Receptor for Advanced Glycosylation End products (RAGE) |
rat, mouse |
Antigen (Clone) IgG |
Distributor (Producer) |
Immunogen (Epitope) |
Host (Formulation) |
Methods glossary |
Specificity |
Reactivity top |
RAGE (poly) IgG
|
US Biological
cat# R1031-10D |
immunogen = synthesized phosphopeptide (KLH conjugated) derived from human Akt around the phosphorylation site of serine 473 (Q-F-SP-Y-S) |
polyclonal rabbit (liquid, PBS, glycerol, azide, affinity purified) |
ELISA, WB |
recognizes endogenous levels of RAGE |
human |
RAGE (9A11) (mono) IgG1
|
Santa Cruz
Cat# sc-80653 |
immunogen = aa 22-342 of mature human RAGE
|
monoclonal mouse (liquid, PBS, gelatin, azide) |
IF, WB |
RAGE |
human |
RAGE (A11) (mono) IgG2a
|
Santa Cruz
Cat# sc-80652 |
immunogen = truncated extracellular domain of human RAGE
|
monoclonal mouse (liquid, PBS, gelatin, azide) |
IF(C), IH(P), IP, WB |
RAGE, natural and recombinant |
human |
RAGE (C-20) (poly) IgG
|
Santa Cruz
Cat# sc-8229 Cat# sc-8229P (peptide) |
immunogen = peptide mapping at the C-terminus of human RAGE |
polyclonal goat (liquid, PBS, gelatin, azide, affinity purified) |
IF, IP, WB |
RAGE |
human, rat, mouse |
RAGE (D-5) (mono) IgG1
|
Santa Cruz
Cat# sc-74535 |
immunogen = aa 1-300 of human RAGE
|
monoclonal mouse (liquid, PBS, gelatin, azide) |
ELISA, IF, IP, WB |
RAGE |
human, rat, mouse |
RAGE (E-1) (mono) IgG1
|
Santa Cruz
Cat# sc-74473 |
immunogen = aa 1-300 of human RAGE
|
monoclonal mouse (liquid, PBS, gelatin, azide) |
ELISA, IF, IP, WB |
RAGE |
human, rat, mouse |
RAGE (H-300) (poly)
|
Santa Cruz
Cat# sc-5563 |
immunogen = aa 1-300 mapping at the N-terminus of human RAGE |
polyclonal rabbit (liquid, PBS, azide, gelatin) |
IF, IP, WB |
RAGE |
human, rat, mouse |
RAGE (M-20) (poly) IgG
|
Santa Cruz
Cat# sc-8231 Cat# sc-8231P (peptide) |
immunogen = peptide mapping at the C-terminus of mouse RAGE |
polyclonal goat (liquid, PBS, gelatin, azide, affinity purified) |
IF, IP, WB |
RAGE |
rat, mouse |
RAGE (N-16) (poly) IgG
|
Santa Cruz
Cat# sc-8230 Cat# sc-8230 P (peptide) |
immunogen = peptide mapping at the N-terminus of human RAGE |
polyclonal goat (liquid, PBS, gelatin, azide, affinity purified) |
IF, IP, WB |
RAGE |
human, rat, mouse, cow, mammal (various) |
RAGE (R12Y7) (mono) IgG2b
|
Santa Cruz
Cat# sc-80312 Cat# sc-80312L (azide-free) |
immunogen = extracellular domain of human RAGE
|
monoclonal mouse (liquid, PBS, gelatin, azide) |
WB |
RAGE |
human, rat, mouse |