|
Antibodies to β-galactosidase
|
| From the Manufacturers
|
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
β-Galactosidase
(2E9)
IgG1
|
Abcam
Cat# ab10074 |
immunogen = full length
β-Galactosidase expressed in E. coli |
monoclonal mouse
(liquid, PBS, azide, protein A purified) |
IA |
β-galactosidase |
E. Coli |
β-Galactosidase
(BG-02)
IgG1
|
Abcam
cat# ab1047 |
immunogen = purified recombinant full length native β-Galactosidase from E. coli |
monoclonal mouse
(liquid, PBS, azide, purified by ppt methods) |
IC, IF, WB |
recognizes a non-catalytic centre epitope of β-galactosidase |
E. Coli |
β-Galactosidase
(BGAL01)
IgG1
|
Abcam
Cat# ab3291 |
immunogen = full length
β-Galactosidase from E. coli |
monoclonal mouse
(liquid, PBS, BSA, azide, protein G purified) |
IP, WB |
β-galactosidase |
E. Coli |
β-Galactosidase
(DC1 4C7/HD4C7)
IgG1
|
Abcam
Cat# ab116 |
immunogen =
β-Galactosidase EC.3.2.1.23 from E. coli |
monoclonal mouse
(liquid, PBS, protein A purified) |
ELISA, WB |
β-galactosidase |
E. Coli |
β-Galactosidase
(DC2 12H4)
IgG1
|
Abcam
Cat# ab18471 |
immunogen =
β-Galactosidase EC.3.2.1.23 from E. coli |
monoclonal mouse
(liquid, PBS, no preservative) |
ELISA |
β-galactosidase |
E. Coli |
β-Galactosidase
(DC3 10D6)
IgG1
|
Abcam
Cat# ab20164 |
immunogen =
β-Galactosidase EC 3.2.1.23 from E. coli |
monoclonal mouse
(liquid, PBS, no preservative) |
ELISA |
β-galactosidase |
|
β-Galactosidase
(poly)
IgG
|
Abcam
Cat# ab616 |
immunogen = recombinant full length β-Galactosidase from E. coli |
polyclonal rabbit
(liquid, purified Ig, KPBS, BSA, glycerol, azide) |
ELISA, IC, IF, IH(F, P, V), IP, WB |
β-galactosidase from E.coli showing a 464 kDa band (tetramer) under non-reducing conditions of SDS-PAGE and 116 kDa band under reducing conditions. |
human, rat, mouse, E. Coli |
β-Galactosidase
(poly)
IgY
|
Abcam
cat# ab9361 |
immunogen = purified recombinant full length native β-Galactosidase from E. coli |
polyclonal chicken
(liquid, PBS, azide, affinity purified) |
ELISA, IC, IEP, IF, IH(F, P, V) |
β-galactosidase |
mouse, E. Coli, drosophila |
β-Galactosidase
(poly)
IgG
|
Abcam
Cat# ab6641 |
immunogen = full length β-Galactosidase from E. coli |
polyclonal rabbit
(liquid, purified, KPBS, BSA, glycerol, azide, conj. FITC) |
IC, IF, IH(C) |
β-galactosidase from E.coli showing a 464 kDa band (tetramer) under non-reducing conditions of SDS-PAGE and 116 kDa band under reducing conditions. |
mouse, E. Coli
(possible mouse) |
β-Galactosidase
(poly)
IgG
|
Abcam
Cat# ab6645 |
immunogen = full length β-Galactosidase from E. coli |
polyclonal rabbit
(liquid, PBS, azide, conj. biotin) |
DB, ELISA, IH(F), IM, IP, WB |
β-galactosidase |
mouse, E. Coli |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
β-Galactosidase
(poly)
IgG
|
Abcam
Cat# ab6646 |
immunogen = full length β-Galactosidase from E. coli |
polyclonal rabbit
(liquid, purified, KPBS, gentamicin sulfate, conj. HRP) |
DB, ELISA, IH(F), IM, IP, WB |
β-galactosidase |
E. Coli |
β-Galactosidase
(poly)
IgG
|
Abcam
Cat# ab4761 |
immunogen = purified full length native β-Galactosidase from E. coli |
polyclonal rabbit
(liquid, PBS, BSA, azide) |
IH(P), WB |
recombinant β-galactosidase in WB |
E. Coli |
β-Galactosidase
(poly)
IgG
|
Abcam
Cat# ab12081 |
immunogen = recombinant full length β-Galactosidase from E. coli |
polyclonal goat
(liquid, PBS, azide) |
WB |
β-galactosidase (116kDa) |
E. Coli |
β-Galactosidase
(poly)
IgY
|
Abcam
Cat# ab36647 |
immunogen = synthetic peptide, corresp. to aa1-1024 of E. coli β-Galactosidase |
polyclonal chicken
(liquid, PBS, no preservative, affinity purified) |
WB |
β-galactosidase |
E. Coli |
β-Galactosidase
(mono)
IgG1
|
AnaSpec
Cat# 53244 |
immunogen = E.Coli β-galactosidase |
monoclonal mouse
(ascites, protein-G purified, PBS, BSA, azide) |
IP, WB |
β-galactosidase |
non-specific |
β-Galactosidase
(DC1-4C7)
IgG1
|
Chemicon and Upstate are Millipore
MAB3468
Reference:
Jäderstad, 2010 |
immunogen = β-Galactosidase from E. coli |
monoclonal mouse
(liquid, ascites, no preservative) |
ELISA, WB |
reacts with Beta- Galactosidase |
|
β-Galactosidase
(poly)
|
Chemicon and Upstate are Millipore
AB1211-5MG |
immunogen = β-Galactosidase from E. coli |
polyclonal rabbit
(liquid, purified Ig, potassium phosphate buffer, saline, azide) |
ELISA, IH, IP, WB |
reacts with Beta- Galactosidase (E. coli). Monospecific by IEP. Some cross reactivity with Beta Galactosidase from other species may occur |
E. Coli, various vertebrate |
β-Galactosidase
(poly)
|
Chemicon and Upstate are Millipore
AB986 |
immunogen = β-Galactosidase from E. coli |
polyclonal rabbit
(antiserum, delipidized, defibrinized, lyophilized) |
ELISA, IH, IP, WB |
reacts with Beta- Galactosidase (E. coli). Monospecific by IEP. Some cross reactivity with Beta Galactosidase from other species |
E. Coli, various vertebrate |
β-Galactosidase
(poly)
|
LifeSpan Biosciences, Inc.
Cat# LS-C32044
available in the USA only |
immunogen = synthetic peptide |
polyclonal chicken
(affinity purified) |
WB |
β-Galactosidase |
(bacteria) |
β-Galactosidase
(poly)
IgG
|
MP Biomedicals
Cappel
Cat# 55978 |
immunogen = β-Galactosidase, covalently coupled to 2ml of Sepharose-4B |
polyclonal rabbit
(chromatography gel in 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, 10% glycerol) |
purification |
reacts with β- Galactosidase (no cross to E. coli) |
|
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
β-Galactosidase
(poly)
IgG
|
MP Biomedicals
Cappel
Cat# 55976
Reference:
Breunig, 2007 |
immunogen = β-Galactosidase from E. coli |
polyclonal rabbit
(liquid, purified, delipidized, saline, sodium phosphate) |
DB, ELISA, EMSA, ID, IF, IH, WB |
reacts with β- Galactosidase; specially prepared for the detection and purification of recomb. proteins |
|
β-Galactosidase
(mono)
IgG1
|
Sigma Aldrich
G 4644
|
immunogen = β-Galactosidase purified from E. coli |
monoclonal mouse
(ascites, Ig fraction, PBS, no preservative, lyophilized) |
WB |
reacts only with β-galactosidase from E. coli, no
reactivity is observed with β-galactosidase from other
sources |
E. Coli |
β-Galactosidase
(GAL-13)
IgG1
|
Sigma Aldrich
G 8021
|
immunogen = β-Galactosidase purified from E. coli |
monoclonal mouse
(ascites fluid, azide) |
DB, ELISA, IC |
reacts with soluble
β-d-galactosidase and retains the enzymatic activi ty.
The antibody reacts with the native enzyme when
coated on solid phase in ELISA or an immunodot blot |
E. Coli |
β-Galactosidase
(GAL-13)
IgG1
|
Sigma Aldrich
B0271
|
immunogen = β-Galactosidase purified from E. coli |
monoclonal mouse
(purified Ig, aqueous solution, PBS, BSA, azide, biotin conj.) |
DB, ELISA |
reacts with soluble
β-d-galactosidase and retains the enzymatic activi ty.
The antibody reacts with the native enzyme when
coated on solid phase in ELISA or an immunodot blot |
E. Coli |
β-Galactosidase
(GAL-40)
IgG1
|
Sigma Aldrich
G 6282
|
immunogen = β-Galactosidase purified from E. coli |
monoclonal mouse
(ascites fluid, azide) |
DB, ELISA, IB, IC |
reacts with native and denatured-reduced E. coli β-galactosidase
(116 kD); may be used
for detection of β-galactosidase expressed by E. coli
lacZ gene encoded in many cloned gene sequences,
and serves as an indicator for fusion proteins encoded
by an inserted DNA sequence |
E. Coli |
β-Galactosidase
(2B35)
IgG1
|
US Biological
Cat# G1041-07 |
immunogen =
β-Galactosidase expressed in E.coli |
monoclonal mouse
(liquid, PBS, azide, protein A purified) |
ELISA |
β-galactosidase of E. coli and Aspergilllus oryzae |
E. Coli
(<10% bovine) |
β-Galactosidase
(3A11)
IgG1
|
US Biological
Cat# G1041-02 |
immunogen = recombinant
β-Galactosidase protein |
monoclonal mouse
(liquid, ascites, HEPES, NaCl, BSA, glycerol, no preservative) |
ELISA, IC, IF, IP, WB |
detects β-galactosidase fusion proteins overexpressed in cells; does not recognize any other overexpressed protein |
various species |
β-Galactosidase
(3F184)
IgG1
|
US Biological
Cat# G1041-08
Cat# G1041-08X (without BSA and azide) |
immunogen =
β-Galactosidase expressed in E.coli |
monoclonal mouse
(liquid, PBS, BSA, azide, protein G purified) |
IB, IP, WB |
β-galactosidase of E. coli |
E. Coli, various species |
β-Galactosidase
(3H21)
IgM
|
US Biological
Cat# G1041-04 |
immunogen = recombinant purified
β-Galactosidase expressed in E.coli |
monoclonal mouse
(liquid, ascites, PBS, azide, glycerol) |
ELISA, WB |
recognizes β-galactosidase protein (~16kD). In ELISA, reacts with the soluble and native enzyme coated on the plate. Does not detect the denatured or reduced proteins in WB |
E. Coli |
β-Galactosidase
(3H402)
IgG1
|
US Biological
Cat# G1041-09 |
immunogen =
β-Galactosidase expressed in E.coli |
monoclonal mouse
(liquid, ascites, no preservative) |
ELISA, WB |
β-galactosidase |
E. Coli |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
β-Galactosidase
(6A87)
IgG2b
|
US Biological
Cat# G1041-07B |
immunogen =
β-Galactosidase expressed in E.coli |
monoclonal mouse
(liquid, PBS, azide, purified) |
ELISA |
β-galactosidase |
E. Coli |
β-Galactosidase
(poly)
IgG
|
US Biological
Cat# G1041-40 |
immunogen =
β-Galactosidase expressed in E.coli |
polyclonal rabbit
(liquid, serum, PBS, glycerol, azide, purified by delipidated and defibrinated) |
DB, ELISA, IP, WB |
β-galactosidase |
E. Coli |
β-Galactosidase
(poly)
IgG
|
US Biological
Cat# G1041-41 |
immunogen =
β-Galactosidase expressed in E.coli |
polyclonal rabbit
(liquid, PBS, glycerol, azide, highly purified) |
DB, ELISA, IF, IH(F, P), IP, WB |
β-galactosidase (~117kDa) |
E. Coli |
β-Galactosidase
(poly)
IgY
|
US Biological
Cat# G1041-11 |
immunogen = full length
β-Galactosidase |
polyclonal chicken
(liquid, PBS, no preservative, glycerol free, affinity purified) |
ELISA, WB |
β-galactosidase |
human, rat, mouse, E. Coli |
β-Galactosidase
(poly)
IgY
|
US Biological
Cat# G1041-12 |
immunogen = full length
β-Galactosidase |
polyclonal chicken
(liquid, PBS, glycerol, affinity purified) |
WB |
bacterial β-galactosidase |
human, rat, mouse, E. Coli |
β-Galactosidase
(poly)
IgG
|
US Biological
Cat# G1041-05F |
immunogen = recombinant purified E. coli
β-Galactosidase |
polyclonal rabbit
(liquid, serum, PBS, azide, glycerol, purified) |
ELISA, WB |
β-galactosidase from E. coli |
E. Coli |
β-Galactosidase
(poly)
IgG
|
US Biological
Cat# G1041-02A |
immunogen = E. coli
β-Galactosidase |
polyclonal rabbit
(liquid, PBS, azide, protein G purified) |
ELISA, WB |
β-galactosidase from E. coli showing a 464kD band (tetramer) under non-reducing conditions of SDS-PAGE and 116kD band under reducing conditions. |
E. Coli |
β-Galactosidase
(poly)
IgG
|
US Biological
Cat# G1041-10 |
immunogen = purified E. coli
β-Galactosidase |
polyclonal rabbit
(lyophilized, PBS, no preservative, affinity purified) |
ELISA; not WB |
β-galactosidase |
human, E. Coli |
β-Galactosidase
(poly)
IgG
|
US Biological
Cat# G1041-05B |
immunogen = recombinant purified E. coli
β-Galactosidase |
polyclonal rabbit
(liquid, PBS, azide, glycerol, purified) |
ELISA, WB |
β-galactosidase from E. coli |
E. Coli |
β-Galactosidase
(poly)
IgG
|
US Biological
Cat# G1041-42 |
immunogen = affinity purified
β-Galactosidase expressed in E.coli |
polyclonal rabbit
(liquid, PBS, BSA, glycerol, azide, highly purified, conj. biotin) |
ELISA, IF, IH(F, P, V), WB |
recognizes E. coli β-galactosidase (464kDa) |
E. Coli |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
β-Galactosidase
(poly)
IgG
|
US Biological
Cat# G1041-05C |
immunogen = recombinant purified E. coli
β-Galactosidase |
polyclonal rabbit
(liquid, PBS, azide, glycerol, purified, conj. biotin) |
ELISA, WB |
β-galactosidase from E. coli |
E. Coli |
β-Galactosidase
(poly)
IgG
|
US Biological
Cat# G1041-05D |
immunogen = recombinant purified E. coli
β-Galactosidase |
polyclonal rabbit
(liquid, PBS, azide, glycerol, purified, conj. FITC) |
IF |
β-galactosidase from E. coli |
E. Coli |
β-Galactosidase
(poly)
IgG
|
US Biological
Cat# G1041-43 |
immunogen = affinity purified
β-Galactosidase expressed in E.coli |
polyclonal rabbit
(liquid or lyophilized, PBS, BSA, glycerol, gentamicin sulfate, affinity purified, conj. HRP) |
ELISA, IH(F, P, V), IP, WB |
recognizes E. coli β-galactosidase (464kDa) |
E. Coli
(expect other sources) |
β-Galactosidase
(poly)
IgG
|
US Biological
Cat# G1041-05E |
immunogen = recombinant purified E. coli
β-Galactosidase |
polyclonal rabbit
(liquid, PBS, thimerosal, glycerol, purified, conj. HRP) |
ELISA |
β-galactosidase from E. coli |
E. Coli |
β-Galactosidase
(poly)
IgG
|
US Biological
Cat# G1041-05G |
immunogen = recombinant purified E. coli
β-Galactosidase |
polyclonal rabbit
(liquid, PBS, thimerosal, glycerol, purified, conj. sepharose) |
IP |
β-galactosidase from E. coli |
E. Coli |
β-Galactosidase (40-1A)
(mono)
IgG1
|
Santa Cruz
Cat# sc-65670 |
immunogen = full length native β-Galactosidase of E. coli origin |
monoclonal mouse
(liquid, PBS, azide, gelatin) |
ELISA, IF, IP, WB |
β-galactosidase |
E. Coli |
β-Galactosidase (BG-02)
(mono)
IgG1
|
Santa Cruz
Cat# sc-51599 |
immunogen = β-Galactosidase purified from E. coli |
monoclonal mouse
(liquid, PBS, azide, gelatin) |
IF, WB |
detection of non-catalytic center epitope of β-galactosidase |
E. Coli |
β-Galactosidase (BGAL01)
(mono)
IgG1
|
Santa Cruz
Cat# sc-57739 |
immunogen = full length native β-Galactosidase of E. coli |
monoclonal mouse
(liquid, PBS, BSA, azide, gelatin) |
ELISA, IP, WB |
detection of non-catalytic center epitope of β-galactosidase |
E. Coli |
β-Galactosidase (SPM373)
(mono)
IgG1
|
Santa Cruz
Cat# sc-56394 |
immunogen = β-Galactosidase of E. coli |
monoclonal mouse
(liquid, PBS, BSA, azide, gelatin) |
IP, WB |
β-galactosidase |
E. Coli |
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