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Home: Research: Compendia: Antibodies

Antibody Directory


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Search Results:		Antigen Class=FLAG, ECS and other tags

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	1 to 12 of 12 results


	Important Notice: We are striving to include as many companies or products as possible, but we do not make a claim to being comprehensive. We may inadvertently omit specific companies or products due to a lack of information.

Antibodies to FLAG, ECS and other tag proteins
From the Manufacturers
Antigen (Clone) IgG	Distributor (Producer)	Immunogen (Epitope)	Host (Formulation)	Methods glossary	Specificity	Reactivity top
DYKDDDDK tag (poly) IgG	Novus Biologicals Cat# NB100-63146	immunogen = keyhole limpet haemocyanin conjugated Enterokinase cleavage site peptide DYKDDDDK	polyclonal rabbit (liquid, antisera, phosphate, saline, azide, affinity purifued)	ELISA, WB	specific for the hydrophilic epitope tagging octapeptide sequence DYKDDDDK (Asp-Tyr-Lys-Asp- Asp-Asp-Asp-Lys), when fused to either the amino- or carboxyl- terminus of target proteins	human, rat, mouse (others not tested)
ECS (poly)	Bethyl Labs Cat# A190-102A Reference: Sato, 2007 (unspecified formulation)	immunogen = ECS, Enterokinase Cleavage Site (xxxDDDDK) conjugated to KLH	polyclonal rabbit (affinity isolated, PBS, azide; also available as affinity purified and HRP-conj., A190-102P)	ELISA, IC, WB	reacts with FLAGTM
ECS (poly)	Bethyl Labs Cat# A190-202A	immunogen = ECS, Enterokinase Cleavage Site (xxxDDDDK) conjugated to KLH	polyclonal rabbit (phosphate, saline, azide)	ELISA, IC, WB	reacts with FLAGTM
FLAG (2EL-1B11) IgG₁	Chemicon and Upstate are Millipore Cat# MAB3118 Reference: Pridgeon, 2007	immunogen = Synthetic peptide (Cys)-DYKDDDK, coupled to ovalbumin	monoclonal mouse (liquid, ascites, no preservative)	ELISA, IC(C), IP, WB	Reacts with both N-terminal and C-terminal fusion proteins and does not require Ca++ for binding. Reacts better in most cases than M2 or M4 FLAG clones	various vertebrate (others not tested)
FLAG (M2) IgG₁	Sigma Aldrich Cat# A9469 References: Wakabayashi, 2009 Serneels, 2009 Tsuda, 2008 Osawa, 2008 Sato, 2007 (all, unspecified formulation)		monoclonal mouse (purified Ig, buffered aqueous glycerol solution, TBS, glycerol, stabilizer, preservative, AP conj. also available as HRP conj., A8592 or biotin labeled, F9291)	DB, ELISA, WB	Binds to the FLAG epitope wherever it is located in the fusion protein: amino-terminal, Met amino-terminal, carboxy-terminal, or internal. Binding is not Ca2+-dependent; useful in detection of FLAG fusion proteins expressed in murine host	rat, mouse, various species
FLAG (M2) IgG₁	Sigma Aldrich Cat# A9594		monoclonal mouse (purified Ig, buffered aqueous glycerol solution, PBS, BSA, preservative, Cy3™)	IF(C)	Binds to the FLAG epitope wherever it is located in the fusion protein: amino-terminal, Met amino-terminal, carboxy-terminal, or internal. Binding is not Ca2+-dependent; useful in detection of FLAG fusion proteins expressed in murine host	rat, mouse, various species
FLAG (M2) IgG₁	Sigma Aldrich Cat# F4049 Reference: (for IF, but unspecified conjugation, Crippa, 2010 )		monoclonal mouse (purified Ig, buffered aqueous solution, PBS, BSA, azide, FITC)	IF(C)	Binds to the FLAG epitope wherever it is located in the fusion protein: amino-terminal, Met amino-terminal, carboxy-terminal, or internal. Binding is not Ca2+-dependent; useful in detection of FLAG fusion proteins expressed in murine host	rat, mouse, various species
FLAG (M2) IgG₁	Sigma Aldrich Cat# P2983		monoclonal mouse (High Sensitivity, M2 coated 96-well plates)	ELISA	Binds to the FLAG epitope wherever it is located in the fusion protein: amino-terminal, Met amino-terminal, carboxy-terminal, or internal. Suitable for screening for expression, study of protein:protein interactions and ELISA assays	various species
FLAG (M2) IgG₁	Sigma Aldrich Cat# F1804		monoclonal mouse (affinity isolated, buffered aqueous solution, phosphate, glycerol, no antimicrobial preservatives)	IB, IC, IH, IP	Binds to the FLAG epitope wherever it is located in the fusion protein: amino-terminal, Met amino-terminal, carboxy-terminal, or internal. Binding is not Ca2+-dependent; optimized for single banded detection of FLAG fusion proteins in mammalian, plant, and bacterial expression systems	various species
FLAG (M2) IgG₁	Sigma Aldrich Cat# F3165 Reference: (Kuhn, 2010, for IP, but see also Cat# F1804)		monoclonal mouse (purified IgG, buffered aqueous solution, phosphate, saline, azide)	ELISA, IP, WB	Binds to the FLAG epitope wherever it is located in the fusion protein: amino-terminal, Met amino-terminal, carboxy-terminal, or internal. Binding is not Ca2+-dependent	various species
Antigen (Clone) IgG	Distributor (Producer)	Immunogen (Epitope)	Host (Formulation)	Methods glossary	Specificity	Reactivity top
FLAG (SIG1-25) IgG₁	Sigma Aldrich Cat# F2555	immunogen = the FLAG™ peptide sequence conjugated to KLH	monoclonal rabbit (ascites fluid, azide)	ELISA, IB, IF	reacts with N-terminal and C-terminal FLAG fusion proteins.	various species
FLAG (poly) IgG	Sigma Aldrich Cat# F7425 References: Li H, 2010 Maruyama, H, 2010 He, 2009	immunogen = Synthetic FLAG sequence containing peptide DYKDDDDK-GC conjugated through a terminal cysteine thiol to maleimide-activated KLH	polyclonal rabbit (affinity purified, buffered aqueous solution, IgG, PBS, BSA, azide)	DB, IF, WB	recognizes the FLAG epitope located on FLAG fusion proteins; reacts with Nterminal, N-terminal-Met, and C-terminal FLAG fusion proteins	non-specific




	1 to 12 of 12 results





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