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Keywords:
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Search Results:
Antigen Class
=histones
Narrow Results
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Method:
Species Reactivity:
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----------------
all monoclonal
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Abcam Ltd
AnaSpec, Inc
Cell Signaling Techn
CHEMICON INTERNATION
Novus Biologicals, I
Upstate Biotechnolog
exclude selected host
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1 to 28 of 28 results
Important Notice:
We are striving to include as many companies or products as possible, but we do not make a claim to being comprehensive. We may inadvertently omit specific companies or products due to a lack of information.
Antibodies to histone proteins
From the Manufacturers
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity
Reactivity
top
acetyl-histone H3
(poly)
Upstate and Chemicon are Millipore
cat# 06-599
immunogen = KLH-conjugated peptide corresp. to a.a. 1-20 of Tetrahymena histone H3(ARTKQTAR[K*] STGG[K*] APRKQLC), where K* is acetylated
polyclonal rabbit
(liquid, purified, Tris-glycine, saline, azide)
ChIP, IC, IP, WB
specific for acetylated histone H3
human, mouse, tetrahymena
acetyl-histone H4
(poly)
Upstate and Chemicon are Millipore
cat# 06-866
Reference:
Stante, 2009
immunogen = KLH-conjugated peptide corresp. to a.a. 2-19 of Tetrahymena histone H4 (AGGAcKGGAcKGM GAcKVGAAcKRHS-C), acetylated on lysines 5, 8, 12 and 16
polyclonal rabbit
(liquid, whole antiserum, unpurified, azide)
ChIP, WB
specific for acetylated histone H4, 10 kDa; weakly cross-reacts with acetylated histone H2B from HeLa cells
human, tetrahymena, eukaryote
Dimethyl-histone H3 (Lys9)
(poly)
IgG
Upstate and Chemicon are Millipore
cat# 17-648
References:
Peleg, 2010
Gupta, 2010
immunogen = a KLH-conjugated, branched synthetic peptide containing the sequence ..Rme2KSTG.., in which me2K corresponds to dimethyl-lysine at residue 9 of human histone H3
polyclonal rabbit
(liquid, rabbit serum, azide)
ChIP, WB
specific for Dimethyl-Histone H3 (Lys9)
human, mouse, mammal (various)
H2A.X
(poly)
IgG
Cell Signaling
Cat# 2595
immunogen = synthetic peptide (KLH-coupled) corresp. to residues near the carboxy-terminus of human histone H2A.X
polyclonal rabbit
(liquid, affinity purified, HEPES, saline, BSA, glycerol)
IC, IF, WB
recognizes endogenous levels of histone H2A.X independent of phosphorylation and ubiquitination
monkey, human
H2A.X
(poly)
IgG
Upstate and Chemicon are Millipore
Cat# 07-627
immunogen = synthetic peptide (C-SATVGPK APSGGKKA) corresp. to aa 121-135 of human histone H2A.X
polyclonal rabbit
(liquid, antiserum, azide, glycerol)
WB
recognizes histone H2A.X
various vertebrate
H2A.X phos Ser139
(20E3)
IgG
Cell Signaling
Cat# 9718
immunogen = synthetic phospho-peptide (KLH-coupled) corresp. to residues surrounding Ser139 of human H2A.X
monoclonal rabbit
(liquid, HEPES, saline, BSA, glycerol
Also available
Alexa Fluor® 488, Cat# 9719
Alexa Fluor® 647, Cat# 9720)
FC, IC, IF,
IH(P)
, WB
detects histone H2A.X phospho. at Ser139
human, mouse
H2A.X phos Ser139
(poly)
IgG
Cell Signaling
Cat# 2577
immunogen = synthetic phospho-peptide (KLH-coupled) corresp. to residues surrounding Ser139 of human H2A.X
polyclonal rabbit
(liquid, HEPES, saline, BSA, glycerol)
FC, IC, IF,
IH(P)
, WB
detects endogenous levels of histone H2A.X phospho. at Ser139, 15 kDa
human, rat, mouse
H2A.X phos Ser139
(JBW301)
IgG
1
Upstate and Chemicon are Millipore
Cat# 05-636
Reference:
Matheu, 2007
immunogen = peptide (C-KATQA [pS]QEY) corresp. to amino acids 134-142 of human histone H2A.X
monoclonal mouse
(liquid, protein-G purified, phosphate buffer, azide, glycerol; also available biotin conj., 16-193, or FITC conj., 16-202A)
IC, IF, WB
recognizes histone H2A.X phospho. at Ser139
various vertebrate
H2A.X phos Ser139
(poly)
IgG
Upstate and Chemicon are Millipore
Cat# 07-164
immunogen = synthetic peptide (C-KATQA [pS]QEY) corresp. to aa 134-142 of human histone H2A.X
polyclonal rabbit
(liquid, protein-A purified, Tris, NaCl, azide, glycerol)
IP, WB
recognizes histone H2A.X phospho. at Ser139
various vertebrate
Histone H1
(mono)
IgG
2a
AnaSpec
Cat# 53553
immunogen = a nuclei of myeloid leukemia biopsy cells
monoclonal mouse
(liquid, ascites, protein-A purified, PBS, BSA, azide)
IF,
IH(P)
detects Histone H1
human
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity
Reactivity
top
Histone H2B
(poly)
IgG
Upstate and Chemicon are Millipore
Cat# 07-371
immunogen = KLH-conjugated, synthetic peptide corresponding to amino acids 118-126 (CG-AVTKYTSSK) of human Histone H2B
polyclonal rabbit
(liquid, purified, Tris-glycine, saline, glycerol, azide)
ChIP, WB
Recognizes and is specific for Histone H2B, MW ~15 kDa
human, S. cerevisiae, xenopus, chicken
Histone H2B acetyl
(poly)
IgG
Upstate and Chemicon are Millipore
Cat# 07-373
immunogen = synthetic peptide in which there is an acetyl-lysine at residues 5, 12, 15 and 20 of human Histone H2B
polyclonal rabbit
(liquid, Tris-glycine, saline, glycerol, azide)
WB
specific for acetylated histone H2B, 15 kDa
human
Histone H2B acetyl Lys12
(poly)
IgG
Upstate and Chemicon are Millipore
Cat# 07-336
immunogen = peptide APAPK AcKGSKKAITK corresponding to 7-20 amino acid of human Histone H2B member F
polyclonal rabbit
(liquid, antiserum, glycerol, azide)
WB
specific for acetyl-histone H2B (Lys12), 15 kDa
human
Histone H2B acetyl Lys120
(poly)
IgG
Upstate and Chemicon are Millipore
Cat# 07-564
immunogen = peptide containing the sequence VTacKYT in which acK corresponds to acetylated lysine 120 of human histone H2B
polyclonal rabbit
(liquid, Tris-glycine, glycerol, azide)
DB, WB
specific for Histone H2B acetylated on lysine 120, ~15 kDa
human
Histone H2B acetyl Lys15
(poly)
IgG
Upstate and Chemicon are Millipore
Cat# 07-343
immunogen = peptide containing the seqeunce GSAcKKAI in which AcK corresponds to acetyl-lysine at residue 15 of human Histone H2B
polyclonal rabbit
(liquid, antiserum, glycerol, azide)
WB
specific for acetyl-histone H2B (Lys15), 15 kDa
human
Histone H2B acetyl Lys20
(poly)
IgG
Upstate and Chemicon are Millipore
Cat# 07-347
immunogen = peptide containing AITAcKAQ in which AcK corresponds to acetyl-lysine at residue 20 of human Histone H2B
polyclonal rabbit
(liquid, antiserum, glycerol, azide)
WB
specific for acetyl-histone H2B (Lys20), 15 kDa
human
Histone H2B acetyl Lys5
(poly)
IgG
Upstate and Chemicon are Millipore
Cat# 07-382
immunogen = synthetic peptide containing an acetyl-lysine at residue 5 of human Histone H2B
polyclonal rabbit
(liquid, purified, glycerol, azide)
WB
specific for acetyl-histone H2B (Lys5), 14-16 kDa
human, various vertebrate
Histone H2B, phos Ser14
(MC603)
IgG
Upstate and Chemicon are Millipore
Cat# 05-751
immunogen = peptide containing the sequence KGpSKK in which pS corresponds to phospho-serine 14 of human histone H2B
monoclonal rabbit
(liquid, protein-A purified, Tris-glycine, saline, azide, glycerol
Available as FITC conj,
16-251
)
FC;
not
WB
detects histone H2B phosphorylated on Serine 14, MW ~15 kDa
human
(wide range expected based on sequence homology)
Histone H2B, phos Ser14
(poly)
IgG
Upstate and Chemicon are Millipore
Cat# 07-191
immunogen = KLH-conjugated, synthetic peptide corresp. to amino acids 6-16 of human histone H2B (C-SAPAPKKG [pS]KK)
polyclonal rabbit
(liquid, polyclonal IgG, buffer, azide, glycerol)
ELISA, IC, IH, WB
detects phospho-Histone H2B , MW ~15 kDa
human
(broad species cross-reactivity expected based on sequence homology)
Histone H3
(poly)
Novus Biologicals
Cat# NB500-267
immunogen = synthetic peptide corresponding to a sequence within amino acids 100-200 of human Histone H3
polyclonal rabbit
(antisera, PBS, azide)
WB
specific for Histone H3; ~17 kDa band in WB
human, mouse
(expect all mammals, and others)
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity
Reactivity
top
histone H3
(mono)
IgG
Upstate and Chemicon are Millipore
cat# 17-10046
Reference:
Gupta, 2010
immunogen = KLH-conjugated, synthetic peptide corresponding to the C-terminus of human Histone H3
monoclonal rabbit
(liquid, supernatant, azide)
ChIP, WB
recognizes Histone H3 near the C-terminus
human, rat, mouse, S. cerevisiae, chicken
(Broad species cross-reactivity expected due to sequence homology.)
histone H3
(poly)
Upstate and Chemicon are Millipore
cat# 06-755
Reference:
Stante, 2009
immunogen = KLH-conjugated peptide corresponding to amino acids 1-20 of human histone H3 (ARTKQTARKST GGKAPRKQLC)
polyclonal rabbit
(liquid, Protein-A purified, Tris-glycine, saline, azide, glycerol)
ChIP, IC, WB
detects histone H3, Mr 17 kDa. Does not effectively recognize acetylated/phosphor. histone H3.
human, rat, mouse
(peptide sequence identical in wide range of animal and plant species.)
Histone H3 phos Ser10
(kit)
IgG
Chemicon and Upstate are Millipore
Cat# HCS204
Reference:
Damiani, 2008
immunogen = Phospho-histone H3
rabbit
(in vitro toxicology assay, needs HCS imaging system equipped with beam-splitters and filters)
IF
for identifying and quantifying phospho-histone H3(Ser10) expression in cellular imaging applications
various animal
histone H4
(kit)
Upstate and Chemicon are Millipore
cat# 17-295
Reference:
Liu, 2007
uses anti-acetyl Histone H4 polyclonal antibody (06-866)
(liquid, Protein-A Agarose/Salmon Sperm DNA, azide, buffers, EDTA)
ChIP
immuno precipitates acetylated histone H4. Works with all eukaryotic cell types. Quantitative PCR detection is recommended.
non-specific
histone H4
(kit)
Upstate and Chemicon are Millipore
cat# 17-229
uses anti-acetyl Histone H4 polyclonal antibody (06-866)
(liquid, Protein-A Agarose/Salmon Sperm DNA, buffers)
ChIP, IP
immuno precipitates transcriptionally active chromatin using anti-acetyl-histone H4.
non-specific
Histone H4 acetyl K12
(poly)
IgG
Abcam
cat# ab1761
Reference:
Peleg, 2010
immunogen = Synthetic peptide: GKGLGK
Ac
GGAKC.
polyclonal rabbit
(liquid, whole antiserum, azide)
ChIP, ELISA,
IF(C)
,
IH(P)
, IP, WB
specific for Histone H4 acetylated at lysine 12
human, rat, mouse, S. cerevisiae, S. pombe, drosophila
MeCP2
(poly)
IgG
Upstate and Chemicon are Millipore
cat# 17-013
Reference:
Gupta, 2010
immunogen = a KLH-conjugated, synthetic peptide corresponding to amino acids 465-478 of mouse MeCP2 (C-PRPNREEPVDSRTP) with an N-terminal cysteine added for conjugation purposes
polyclonal rabbit
(liquid, rabbit IgG in Tris-Glycine, saline, glycerol, azide)
WB
specific for MeCP2
rat, mouse
Trimethyl-histone H3 (Lys4)
(mono)
IgG
Upstate and Chemicon are Millipore
cat# 17-613
Reference:
Gupta, 2010
immunogen = a BSA-conjugated, synthetic peptide containing the sequence …RT[me3K]QT… in which me3K corresponds to trimethyl-lysine 4 of human histone H3
monoclonal rabbit
(liquid, protein A purified rabbit IgG in Tris-Glycine, saline, glycerol, azide)
ChIP, WB
specific for Trimethyl-Histone H3 (Lys4)
human, mouse, mammal (various)
1 to 28 of 28 results
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