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Antibodies
Antibody Directory
Initial Search
Antigen Class:
-- all --
14-3-3
4E-BP1
4-hydroxy-nonenal
5-HT /serotonin
ABC, ABC transporter
ACC
ACE
Acetylcholine-related
Actin related
ADAM and ADAMTS
Adaptin
Adaptor Protein Complex (AP)
Adenomatus Polyposis Coli /APC
Adenylate cyclase
AFP
AGE and RAGE
Akt and serine/threonine kinases
Alix
Alpha-1-antitrypsin
α-, β-, γ-secretase
AMCase
Amino acids, phosphorylated
AMPAR, NMDAR, GluR1, GluR2
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AMPK
Amyloid-beta related
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AP-2 transcription factor
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APP, APLP
Arc
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ASK
ATPase
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Bassoon
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CD45
CDK1/CDC2 and CDK2
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CD_ except CD11b, CD40, CD45 and CD147
c-Fos and c-Jun
Chemokine receptor
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DCP
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DJ-1
Dlg/Discs-large
Dlx2
DNA/RNA Oxidative Damage
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Doublecortin
DR6
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Dynamin related protein
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eIF /Eukaryotic initiation factor
ECE
ECT, Lipid Metabolism
EEA1
EGF, EGFr
EGR-1 /Zif268
Elav proteins
Elongation Factors
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ERAB
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FADD
FAS-Related
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Fe65
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FKBP12
FKHR/FoxO proteins
FLAG, ECS and other tags
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Futsch
GABA related and Gephyrin
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GAP related
GAPDH /G3PDH
GATA
GFAP
GFP and RFP
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glucocorticoid receptor /GR
Glutamine Synthetase
Go
Golgi matrix/membrane, GM130
Grb-2
GRKs
GSK-3
GST
HA and HIS peptides
Hb9
Heat Shock Proteins
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HES
Histone deacetylase (HDAC)
histones
hnRNP
Homer
Hox
Human nucleii
Huntingtin
Huntington's-related
Iba-1
IFN-γ
IGF Receptor
IgG
Insulin Receptor
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IRF
iron metabolism: transferrin, ferroportin, CP
IRS
Islet
JAK2
JNK/SAPK-1/2/3 and MAPK8-10
Ki-67
Kinesin Heavy Chain
Lamin and Nuclear Pore Complex
Laminin
Lamp
Lck and Lck Ligand
LDL Receptor family
LHX2
LL37 /CAP18
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LTE4
Ly-6 surface proteins
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Matrix Metalloproteinase (MMP)
MeCP2
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myc and V5 epitope tags
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Myosin
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NDUFS4
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NeuN
Neurexin
Neuro D
Neurofib Tangles and p62
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Neurogenin
Neuropeptide Y
Neurophysin Related
NFkB, IkB and related proteins
NG2
NGF, NGF-related
NGFR, Trk-A/B
Nieman Pick C1
Nitrotyrosine
Nkx
NOS
Notch, Jagged, Delta
NOX2
NSE
NUMB and NUMBLike
Occludin
O-GlcNAc
Olfactory Proteins
Oligodendrocyte markers
Optineurin
Orexin
p19
p21 and p27
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p53
PAK
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Parvalbumin
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PDGF, PDGF Receptor
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Phosphorylated Strings
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PINK1
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PKA, PKC and related
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Presenilin Complex, Nicastrin, Ubiquilin
Presenilins
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Proline disulfide isomerase /PDI
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proteolipid protein /PLP
PSA-NCAM
PSD95
PTPs
Rab or Ras related, except Rab3
Rac and Cdc42
RAP
Recoverin
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Retrolinkin
Retromer Complex
Rho
Rhodopsin
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Ryanodine receptor
S-100
S6 Kinase
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SHP2
SIRT1/Sir2 and SIRT2
SNX
SOCS3/CIS3
SOD
Sodium channel, voltage gated
Somatostatin related
Sortilins
SOS-1
Sox
Spectrins
Splicing Factors
SR-BI
Src
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SRF
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Stat1, Stat3 and related proteins
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SUMO
Synaptic Vesicle proteins
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TACE
TAR DNA-binding protein /TDP-43
Tau
Tbr
TdT
TGF
Thioredoxin related
TIA related
Tip60
TLR /Toll-like Receptor
TMP21
TNF
Tom
tPA and PAI-1
TRA-1
Trans-Golgi network /TGN
TRRAP
Tryptophan Hydroxylase
TSG101
Tubulin
TUC (TOAD/Ulip/CRMP)
Tyrosine Hydroxylase
Tyrosine kinase
UbcH
Ubiquitin
Urocortin
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Vav
VCAM
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VEGF and VEGF Receptor
Veli
VGAT
vGluT
von Willebrand factor
Vps35
WNT3
ZO-1
(plus)
Keywords:
any keywords
all keywords
Search Results:
Antigen Class
=CDK1/CDC2 and CDK2
Narrow Results
Host:
Method:
Species Reactivity:
Distributor:
-- select --
goat
mouse
rabbit
----------------
all monoclonal
all polyclonal
all kit
-- select --
DB
ELISA
FC
IC
IF
IF(C)
IH
IH(F)
IH(P)
IP
RIA
WB
and
-- select --
amphibian (various)
Arabidopsis thaliana
bear
bovine
chicken
cow
dog
drosophila
frog
guinea pig
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human
mammal (various)
mink
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mouse
primate (various)
rabbit
rat
rodent (various)
S. pombe
sea star
sheep
various species
woodchuck
xenopus
and
-- select --
Abcam Ltd
AnaSpec, Inc
Biolegend, Inc.
Cell Signaling Techn
CHEMICON INTERNATION
Epitomics, Inc.
LifeSpan Biosciences
Novus Biologicals, I
PharMingen BD Biosci
Phosphosolutions
SANTA CRUZ BIOTECHNO
Serotec Inc
Transduction Laborat
Upstate Biotechnolog
exclude selected host
-- select --
DB
ELISA
FC
IC
IF
IF(C)
IH
IH(F)
IH(P)
IP
RIA
WB
-- select --
amphibian (various)
Arabidopsis thaliana
bear
bovine
chicken
cow
dog
drosophila
frog
guinea pig
hamster
human
mammal (various)
mink
monkey
mouse
primate (various)
rabbit
rat
rodent (various)
S. pombe
sea star
sheep
various species
woodchuck
xenopus
1 to 100 of 149 results
Important Notice:
We are striving to include as many companies or products as possible, but we do not make a claim to being comprehensive. We may inadvertently omit specific companies or products due to a lack of information.
CDK1/CDC2 and CDK2
From the Manufacturers
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity
Reactivity
top
Cdc2
(A17)
IgG
2a
Abcam
Cat# ab18
immunogen = C-terminal fragment starting at aa 85 of Xenopus p34cdc2 expressed in
E. coli
; epitope = residues 220-227 of mouse cdc2, L G T P N N E V
monoclonal mouse
(liquid, PBS, protein A purified)
ELISA,
IH(F, P)
, IP, RIA, WB
cdc2; inhibits the activation of p34cdc2 kinase by cyclins
human, rat, mouse, xenopus, chicken
Cdc2
(POH1)
IgG
2a
Cell Signaling
Cat# 9116
immunogen = recombinant human cdc2 fusion protein
monoclonal mouse
(liquid, HEPES, NaCl, BSA, glycerol, azide)
IF(C)
,
IH(P)
, IP, WB
detects endogenous levels of total cdc2 protein;
not
other cdks
monkey, human
Cdc2
(poly)
Cell Signaling
Cat# 9112
immunogen = synthetic peptide (KLH-coupled) corresp. to residues surrounding Tyr15 of human cdc2
polyclonal rabbit
(liquid, HEPES, NaCl, BSA, glycerol, azide, protein A purified)
WB
endogenous levels of total cdc2 protein
human, rat, mouse
Cdc2
(mono)
IgG
Epitomics
Cat# 1053-1
Immunogen = synthetic peptide corresponding to resiudes of human Cdc2
monoclonal rabbit
(liquid Tris-glycine, glycerol, azide)
FC, IP, WB
Cdc2
human, various species
Cdc2
(mono)
IgG
Epitomics
Cat# 1484-1
Immunogen = synthetic peptide corresponding to residues near the C-terminus of human Cdc2
monoclonal rabbit
(frozen liquid, tris-glycine, NaCl, glycerol, azide)
IH(P)
, WB
34 kDa Cdc2
human, rat, mouse, bovine
Cdc2
(mono)
LifeSpan Biosciences, Inc.
Cat# LS-C5886
available in USA only
immunogen = recombinant human cdc2 fusion protein. apparent MW: 34kD
monoclonal mouse
(ascites)
ELISA, IC, IF, IH, IP, WB
endogenous levels of cdc2;
no
cross reaction with other cdks
monkey, human
(
not
mouse or rat)
Cdc2
(mono)
LifeSpan Biosciences, Inc.
Cat# LS-C5893
available in USA only
immunogen = recombinant protein consisiting of the C-terminal two-thirds of the Xenopus p34cdc2
monoclonal mouse
(protein A purified)
ELISA, IC, IH, IP, WB
endogenous levels of cdc2;
no
cross reaction with other cdks
human, rodent (various), amphibian (various), chicken
Cdc2
(mono)
LifeSpan Biosciences, Inc.
Cat# LS-C5963
available in USA only
immunogen = recombinant protein C-terminal two thirds of Xenopus cdc2 expressed in
E.coli
monoclonal mouse
(protein A purified)
IF, IH, IP, WB
cdk1 (also known as p34cdc2) at 34kD
human, woodchuck, rat, guinea pig, mouse, xenopus, chicken
Cdc2
(mono)
LifeSpan Biosciences, Inc.
Cat# LS-C5965
available in USA only
immunogen = recombinant protein C-terminal two thirds of Xenopus cdc2 expressed in
E.coli
monoclonal mouse
(protein A purified)
IF, IH, IP, WB
cdk1 (also known as p34cdc2) at 34kD
human, woodchuck, rat, guinea pig, mouse, xenopus, chicken
Cdc2
(mono)
LifeSpan Biosciences, Inc.
Cat# LS-C5968
available in USA only
immunogen = purified recombinant human cdk1 (p34cdc2)
monoclonal mouse
(DEAE-column chromatography)
ELISA, IH, IP, WB
cdk1 (also known as p34cdc2) at 34kD
monkey, human, mink, bovine
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity
Reactivity
top
Cdc2
(mono)
LifeSpan Biosciences, Inc.
Cat# LS-C14410
available in USA only
immunogen = Xenopus cdc2 p34; maps near the C-terminus of protein and its core is thought to be L G T P N N E V
monoclonal mouse
(IgG fract.)
IF, IH, IP, WB
cdk1 (also known as p34cdc2) at 34kD;
no
cross-reaction with cdk2/p32 and other cyclin dependent kinases (cdks)
human, rat, mouse, xenopus, chicken
Cdc2
(mono)
LifeSpan Biosciences, Inc.
Cat# LS-C18772
immunogen = recombinant human p34 cdc2 fusion protein.
monoclonal mouse
(protein A purified)
IH, IP, WB
cdk1 (also known as p34cdc2) at 34kD
human, rat, mouse
Cdc2
(mono)
LifeSpan Biosciences, Inc.
Cat# LS-C5890
available in USA only
immunogen = C-terminal fragment starting at aa85 of Xenopus p34cdc2 expressed in
E. coli
; epitope is thought to be residues 220-227 of mouse cdc2, L G T P N N E V
monoclonal mouse
ELISA, IH, IP, WB
cdc2
human, mouse, rodent (various), xenopus, amphibian (various), chicken
Cdc2
(mono)
LifeSpan Biosciences, Inc.
Cat# LS-C5953
available in USA only
immunogen = recombinant human cdk1 (p34cdc2) fusion protein
monoclonal mouse
(protein A purified)
IH, IP, WB
cdk1 (also known as p34cdc2) at 34kD;
no
reaction with other cdks
human, rat, mouse
Cdc2
(mono)
LifeSpan Biosciences, Inc.
Cat# LS-C22029
available in USA only
immunogen = synthetic peptide corresp. to residues in C-terminus of human Cdk2)
monoclonal rabbit
(supernatant)
IC, IP, WB
cdk2
human
Cdc2
(mono)
LifeSpan Biosciences, Inc.
Cat# LS-C26672
monoclonal mouse
IH, WB
cdc2
human
Cdc2
(mono)
LifeSpan Biosciences, Inc.
Cat# LS-C5967
available in USA only
immunogen = purified recombinant human p34 cdc-2 fusion protein
monoclonal mouse
(ascites)
IH(P)
, WB
detects the three bands within the 34kD region corresponding to the p34 protein and it's cleavage products
human
Cdc2
(mono)
LifeSpan Biosciences, Inc.
Cat# LS-C22011
available in USA only
immunogen = synthetic peptide corresp. to residues of human Cdc2
monoclonal rabbit
(supernatant)
IP, WB
cdc2
human
Cdc2
(mono)
LifeSpan Biosciences, Inc.
Cat# LS-C22012
available in USA only
immunogen = a synthetic peptide corresp. to residues near the C-terminus of human Cdc2
monoclonal rabbit
(supernatant)
IH, WB
cdc2
human
Cdc2
(poly)
LifeSpan Biosciences, Inc.
Cat# LS-C18771
immunogen = Cdc2 peptide corresp. to the C-terminus of the human protein conjugated to KLH
polyclonal rabbit
(delipidation and defibrination)
ELISA, IH, IP, WB
cdc2
human
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity
Reactivity
top
Cdc2
(poly)
LifeSpan Biosciences, Inc.
Cat# LS-C5884
available in USA only
immunogen = synthetic peptide corresp. to the C-terminus of the human cdc2 protein (KLH coupled)
polyclonal rabbit
(DEAE-column chromatography)
ELISA, IH, IP, WB
cdc2 protein (34kD);
no
cross-reaction with other related cdks
human, rat, mouse
Cdc2
(poly)
LifeSpan Biosciences, Inc.
Cat# LS-C5961
available in USA only
immunogen = synthetic peptide corresp. to aa 292-297 (Cys-N Q I K K M) of the C-terminus of human cdk1, conj. KLH
polyclonal rabbit
(protein A purified)
ELISA, IF, IP, WB
cyclin-dependent kinase-1 (cdk1, 34kDa);
no
cross-reaction with other members of the cdk family
human, rat, mouse
Cdc2
(poly)
LifeSpan Biosciences, Inc.
Cat# LS-C5964
available in USA only
immunogen = recombinant protein of two-thirds of the C-terminal region of Xenopus cdc2 (cdk1) expressed in
E. coli
polyclonal rabbit
(protein A purified)
ELISA, IP, WB
cyclin-dependent kinase-1 (cdk1, 34kDa);
no
cross-reaction with other members of the cdk family
human, woodchuck, rat, guinea pig, mouse, xenopus, chicken
Cdc2
(poly)
LifeSpan Biosciences, Inc.
Cat# LS-C22028
available in USA only
immunogen = synthetic peptide (C F L S K M L V Y D P A K R I S G K M A L K H P Y F) corresp. to aa263-287 of cdk1/cdc2 (KLH)
polyclonal rabbit
(unpurified serum)
ELISA, IP, WB
cyclin-dependent kinase-1 (cdk1, 34kDa);
no
cross-reaction with other members of the cdk family
human, rat
Cdc2
(poly)
LifeSpan Biosciences, Inc.
Cat# LS-C5885
available in USA only
immunogen = synthetic peptide derived from the sequence of human cdc2 surrounding Tyr15
polyclonal rabbit
(protein A purified)
ELISA, WB
endogenous levels of total cdc2 protein
human, rat, mouse
Cdc2
(poly)
LifeSpan Biosciences, Inc.
Cat# LS-C5952
available in USA only
immunogen = purified synthetic peptide corresp. to the C-terminal region of human cdc2 1 (KLH coupled)
polyclonal rabbit
(DEAE-column chromatography)
IH(F)
, WB;
not
IP
cdc2 at 34kDa
human, mammal (various)
Cdc2
(poly)
LifeSpan Biosciences, Inc.
Cat# LS-C5955
available in USA only
immunogen = 19 residue synthetic peptide (E G V P S T A I R E I S L L K E-G G C) corresp. to the residues 42-57 of the human 34kDa cdc2 kinase 1 coupled to KLH
polyclonal rabbit
(DEAE-column chromatography)
IP, WB
cdc2 at 34kDa and related proteins at 32kDa and 58kDa
human, rat, mouse, sheep, sea star, frog, chicken
Cdc2
(poly)
LifeSpan Biosciences, Inc.
Cat# LS-C5956
available in USA only
immunogen = 19 residue synthetic peptide (E G V P S T A I R E I S L L K E-G G C) corresp. to the residues 42-57 of the human 34kDa cdc2 kinase 1 coupled to KLH
polyclonal rabbit
(protein A purified)
IP, WB
cdc2 at 34kDa
human, rat, mouse, sheep, sea star, frog, chicken, various species
Cdc2
(poly)
LifeSpan Biosciences, Inc.
Cat# LS-C5957
available in USA only
immunogen = peptide corresp. to aa 292-297 (Cys-N Q I K K M) from the C-terminal of human cdk1/p34cdc2 coupled to KLH
polyclonal rabbit
(protein A purified)
IF, IP
cdc2 at 34kDa
human, rat, mouse
Cdc2
(poly)
LifeSpan Biosciences, Inc.
Cat# LS-C5960
available in USA only
immunogen = A 16 residue synthetic peptide E G V P S T A I R E I S L L K E(GGC) based on the human, mouse, rat and hamster 34kD cdc2-encoded protein kinase (residues 42-57) conj. KLH
polyclonal rabbit
(DEAE-column chromatography)
IP, WB
cdc2 at 34kDa; sometimes reactivity to cdc2 42kDa is seen in WB
monkey, human, rat, mouse, hamster, rabbit, dog, bovine
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity
Reactivity
top
Cdc2
(poly)
LifeSpan Biosciences, Inc.
Cat# LS-C5962
available in USA only
immunogen = peptide corresp. to aa 292-297 (Cys-N Q I K K M) from the C-terminal of human cdk1/p34cdc2 conj. KLH
polyclonal rabbit
(protein A purified)
IF, IP
cdc2 at 34kDa
human, rat, mouse
Cdc2
(poly)
LifeSpan Biosciences, Inc.
Cat# LS-C183
immunogen = synthetic peptide
polyclonal rabbit
(HEPES, NaCl, BSA, glycerol, affinity purified)
WB
cdc2 at 34kDa
human, rat, mouse, drosophila
Cdc2
(1A4-1A9)
IgG
1
, κ
Novus Biologicals
Cat# H00000983-M01
supplied by Abnova; available in N. America only
immunogen = full length recombinant CDC2
monoclonal mouse
(liquid, purified, no preservative)
WB
CDC2 - cell division cycle 2, G1 to S and G2 to M
human
(other species not tested)
Cdc2
(1C7)
IgG
1
, κ
Novus Biologicals
Cat#H00000983-M02
supplied by Abnova
available in N. America only
immunogen = full length recombinant CDC2
monoclonal mouse
(liquid, purified, no preservative)
WB
CDC2 - cell division cycle 2, G1 to S and G2 to M
human
(other species not tested)
Cdc2
(1G10)
IgG
mix
, λ
Novus Biologicals
Cat#H00000983-M03
supplied by Abnova
available in N. America only
immunogen = partial recombinant CDC2
monoclonal mouse
(liquid, purified, no preservative)
WB
CDC2 - cell division cycle 2, G1 to S and G2 to M
human, rat, mouse
(other species not tested)
Cdc2
(8F1)
IgG
2a
, λ
Novus Biologicals
Cat#H00000983-M04
supplied by Abnova
available in N. America only
immunogen = partial recombinant CDC2
monoclonal mouse
(liquid, purified, no preservative)
WB
CDC2 - cell division cycle 2, G1 to S and G2 to M
human, rat, mouse
(other species not tested)
Cdc2
(POH-1)
IgG
2a
Novus Biologicals
Cat# NB 500-357
immunogen = recombinant full length human protein
monoclonal mouse
(liquid, azide)
IC,
IH(P)
, IP, WB
CDC2
monkey, human, mink, cow
(
not
mouse, rat, drosophila, S.cerevisiae, S. pombe, or Xenopus; other species not tested)
Cdc2
(poly)
Novus Biologicals
Cat# H00000983-A01
supplied by Abnova
available in N. America only
immunogen = partial recombinant CDC2
polyclonal mouse
(liquid, serum, glycerol, no preservative)
WB
CDC2 - cell division cycle 2, G1 to S and G2 to M
human
(other species not tested)
Cdc2
(poly)
IgG
Novus Biologicals
Cat# NB 600-466
immunogen = synthetic peptide (human) conjugated to KLH
polyclonal rabbit
(liquid, serum, no buffer, azide)
ELISA, IH, WB
detects CDC2;
no
reaction to other related cyclin dependent kinases; inhibits activation of p34cdc2 kinase by cyclins
human, rat, mouse
(other species not tested)
Cdc2
(poly)
IgG
Novus Biologicals
Cat# NB 120-7953
Reference:
Wang, J, 2010
immunogen = C-terminal synthetic peptide (human)
polyclonal rabbit
(liquid, IgG purified, PBS, azide)
IH, IP, WB
detects CDC2 p34
human, rat, mouse
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity
Reactivity
top
Cdc2
(mono)
IgG
2a
Santa Cruz
Cat# sc-51578
immunogen = human recombinant Cdc2 protein
monoclonal mouse
(liquid, PBS, gelatin, azide)
IF,
IH(P)
, IP, WB
Cdc2
human
Cdc2
(mono)
IgG
1
Santa Cruz
Cat# sc-8395
immunogen = aa 6-24 mapping at the N-terminus of cdc2 p34 of human origin
monoclonal mouse
(liquid, PBS, gelatin, azide; blocking peptide sc-8395P)
IF, IP, WB
Cdc2 p34
human, rat, mouse
Cdc2
(mono)
IgG
2a
Santa Cruz
Cat# sc-54
immunogen = aa 224-230, mapping within a central region of human Cdc2 p34
monoclonal mouse
(liquid, PBS, gelatin, azide, affinity purified; blocking peptide sc-54; conj. agarose sc-54AC; conj. FITC sc-54 FITC; conj. rhodamine sc-54 TRICT; conj. HRP sc-54 HRP)
IF,
IH(P)
, IP, WB;
kinase assay
Cdc2 p34
human, mouse, mammal (various), xenopus
Cdc2
(mono)
IgG
1
Santa Cruz
Cat# sc-53216
immunogen = C-terminal fragment starting at Methionine 85 of
Xenopus
Cdc2 p34
monoclonal mouse
(liquid, PBS, gelatin, azide)
WB
Cdc2 p34
xenopus
Cdc2
(mono)
IgG
2b
Santa Cruz
Cat# sc-53217
immunogen = peptide spanning the C-terminal 14 residues of
S. pombe
Cdc2 (A K R A L Q Q N Y L R D F H)
monoclonal mouse
(liquid, PBS, gelatin, azide)
WB
Cdc2 p34
S. pombe
Cdc2
(poly)
IgG
Santa Cruz
Cat# sc-12826
epitope = mapping near the C-terminus of Cdc2 of
Arabidopsis thaliana
origin
polyclonal goat
(liquid, PBS, gelatin, azide; blocking peptide sc-12826P)
IF, WB
Cdc2
Arabidopsis thaliana
Cdc2
(poly)
IgG
Santa Cruz
Cat# sc-954
immunogen = epitope mapping at the C-terminus of Cdc2 p34 of human origin
polyclonal rabbit
(liquid, PBS, gelatin, azide, affinity purified; blocking peptide sc-954)
IF,
IH(P)
, IP, WB
Cdc2 p34
human, rat, mouse, cow, various species
cdc2 (p34)
(POH-1)
IgG
2a
,κ
Biolegend
Cat# 626902
immunogen = recombinant human cdc2 protein
monoclonal mouse
(affinity purified, PBS, azide)
IF,
IH(P)
, IP, WB
detects cdc2
monkey, human, cow, bovine
(
not
with mouse, rat, Xenopus, Drosophila)
Cdc2 (PSTAIRE)
(poly)
IgG
Santa Cruz
Cat# sc-53
immunogen = epitope mapping within the conserved PSTAIRE domain of human Cdc2 p34
polyclonal rabbit
(liquid, PBS, gelatin, azide, affinity purified; blocking peptide sc-53)
IF, IP, WB
Cdc2 p34 and other cyclin dependent kinases containing PSTAIRE motif
human, rat, mouse, drosophila, xenopus, chicken
cdc2 /CDK1
(3E1.1)
IgG
1
Abcam
Cat# ab20151
immunogen = C-terminal fragment, starting from methionine 85 of Xenopus Cdc2
monoclonal mouse
(liquid, PBS, no preservative)
WB
reacts strongly with immunoblotted antigen but does not immunoprecipitate native p34cdc2
bear, xenopus
(other species not tested)
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity
Reactivity
top
cdc2 /CDK1
(E161)
IgG
Abcam
Cat# ab32384
immunogen = synthetic peptide corresp. to residues surrounding Tyr15 of human Cdc2
monoclonal rabbit
(liquid, tris-glycine saline, BSA, glycerol, azide)
IC,
IH(P)
, IP, WB;
not
FACS
detects Cdc2 without phosphorylation on Tyrosine 15; does
not
detect Y15 phosphorylated Cdc2
human, rat, mouse
(expect Cow; other species not tested)
cdc2 /CDK1
(E53)
IgG
Abcam
Cat# ab32030
immunogen = synthetic human peptide
monoclonal rabbit
(liquid, tris-glycine saline, BSA, glycerol, azide)
IP, WB;
not
FACS, ICC, IHC
cdc2
human
(
not
mouse, rat)
cdc2 /CDK1
(POH-1)
IgG
2a
Abcam
Cat# ab8040
immunogen = recombinant full length human protein
monoclonal mouse
(liquid, PBS, azide, protein A purified)
IC,
IH(P)
, IP, WB
Cdc2
monkey, human, mink, cow
(
not
Mouse, Rat, Drosophila melanogaster, S. cerevisiae, S. pombe, Xenopus)
cdc2 /CDK1
(SPM234)
Abcam
Cat# ab17786
immunogen = recombinant fragment from the C-terminal 2/3rds (aa 220-227) of Xenopus laevis cdc2 expressed in
E.coli
monoclonal mouse
(liquid, protein G purified, prediluted)
IH(P)
Cdc2
human, woodchuck, rat, guinea pig, mouse, xenopus, chicken
cdc2 /CDK1
(Y100.4)
IgG
2b
Abcam
Cat# ab5467
immunogen = synthetic C-terminal peptide: A K R A L Q Q N Y L R D F H (Schizosaccharo- myces pombe)
monoclonal mouse
(liquid, PBS, no preservative, protein A purified)
WB
cdc2 (34kDa)
S. pombe
(other species not tested)
cdc2 /CDK1
(YE324)
IgG
Abcam
Cat# ab32094
immunogen = synthetic peptide corresp. to residues near the C-terminus of human Cdc2
monoclonal rabbit
(liquid, tris-glycine saline, BSA, glycerol, azide)
IH(P)
, WB;
not
FACS, IC or IP
Cdc2
human
(
not
mouse, rat)
cdc2 /CDK1
(poly)
IgG
Abcam
Cat# ab7954
immunogen = synthetic peptide from human C- terminal
polyclonal rabbit
(liquid, PBS, azide)
IC,
IH(F)
, IP, WB
cdk2
human, rat, mouse
(other species not tested)
cdc2 /CDK1
(poly)
IgG
Abcam
Cat# ab7953
immunogen = synthetic peptide from human C-terminal
polyclonal rabbit
(liquid, PBS, azide)
IH(P)
, IP, WB
cdc2
human, rat, mouse
cdc2 /CDK1
(poly)
IgG
Abcam
Cat# ab6537
immunogen = synthetic human peptide, conjugated to KLH
polyclonal rabbit
(liquid, whole antisera)
ELISA,
IH(P)
, IP, WB
cdc2;
no
reaction against other related cyclin dependent kinases; inhibits the activation of p34cdc2 kinase by cyclins
human, rat, mouse
cdc2 /CDK1
(poly)
IgG
Abcam
Cat# ab33128
immunogen = synthetic peptide, C F L S K M L V Y D P A K R I S G K M A L K H P Y F corresp. to aa 263/287 of Cdk1, conjugated to KLH,
polyclonal rabbit
(liquid, whole serum, azide)
ELISA, IP, WB
cdk1/cdc2
human, rat, mouse
(expect chicken, cow, cylomolgus)
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity
Reactivity
top
cdc2 /CDK1
(poly)
IgG
Abcam
Cat# ab31687
immunogen = synthetic peptide: E G V P S T A I R E I S L L K E-GGC corresp. to aa 42-57 of human Cdk1, conj. to KLH
polyclonal rabbit
(liquid, tris-glycine saline, glycerol, azide, protein A purified)
IP, WB
Cdc2
human, rat, mouse, sheep, sea star, xenopus, chicken
(expect wide range of species)
cdc2 /CDK1
(poly)
IgG
Abcam
Cat# ab22659
immunogen = synthetic peptide: E G V P S T A I R E I S L L K E(GGC) corresp. to aa 42-57 of human, mouse, rat and hamster Cdc2, with a 3 amino acid (GGC) linker arm attached and the peptide coupled to KLH
polyclonal rabbit
(liquid, borate buffered saline, affinity purified)
IP, WB
Cdc2
monkey, human, rat, mouse, hamster, rabbit, dog, cow
cdc2 /CDK1
(poly)
BD Pharmingen
Cat# 558900
immunogen = synthetic peptide corresp. to aa 292-297 (N Q I K K M) at C-terminal from human cdk1/cdc2
polyclonal rabbit
(liquid, serum, azide)
IP, WB
CDK1 (p34 cdc2);
not
other known cdk proteins
human, mouse
cdc2 /CDK1
(1)
IgG
1
BD Transduction
Cat# 610038 150μg
Cat# 610037 50μg
immunogen = aa 283-297 at C-terminal from human p34 cdc2
monoclonal mouse
(liquid, supernatant, BSA, glycerol, azide, affinity purified)
IF, IH, IP, WB
human, rat, mouse
cdc2 /CDK1 phos Y15
(44)
IgG
1
BD Transduction
Cat# 612307 150μg
Cat# 612306 50μg
immunogen = human cdc2 at phosphorylated Tyr15
monoclonal mouse
(liquid, supernatant, BSA, glycerol, azide, affinity purified)
IF, WB
Cdk1/cdc2 phosphorylated at Y15
human, rat, mouse
Cdc2 no phosY15
(mono)
IgG
Epitomics
Cat# 1161-1
Immunogen = synthetic peptide corresponding to residues surrounding Tyr15 of human Cdc2
monoclonal rabbit
(liquid tris-glycine, glycerol, azide)
IC,
IH(P)
, IP, WB
Cdc2 without phosphorylation on Tyr15.
Not
Y15-phosphorylated Cdc2
human, rat, mouse, bovine
cdc2 p34
(poly)
IgG
AnaSpec
Cat# 53353
immunogen = synthetic peptide derived from the C-terminus of human Cdc2 p34 protein
polyclonal rabbit
(liquid, affinity purified, PBS, BSA, azide)
IH(P)
, IP, WB
detects cdc2 p34
human, rat, mouse
cdc2 paired 15
(poly)
IgG
AnaSpec
Cat# 55025
immunogen = synthetic peptide corresp. to the sequence of human CDC2 around aa. 15 (EGTYGV)
polyclonal rabbit
(liquid, affinity purified, PBS, azide)
WB
detects cdc2
human
cdc2 phos T14
(poly)
IgG
Abcam
Cat# ab4823
immunogen = synthetic peptide derived from the region of the human cyclin-dependent kinase that contains threonine 14 and tyrosine 15
polyclonal rabbit
(liquid, PBs, no preservative, affinity purified)
IP, WB
recognizes Cdk 1 [pTpY14/15] and may recognize other cyclin-dependent kinases phosphorylated on these residues;
no
reactivity to Cdk 4 or Cdk 6
human, rat, mouse
Cdc2 phos Thr14
(poly)
Cell Signaling
Cat# 2543
immunogen = synthetic phosphopeptide (KLH-coupled) corresp. to residues surrounding Thr14 of human cdc2
polyclonal rabbit
(liquid, HEPES, NaCl, BSA, glycerol, azide, protein A purified)
WB
endogenous levels of total cdc2 protein only when phosphorylated at Thr14; may cross react with CDK2 and CDK3
monkey, human, rat, mouse, bovine, drosophila, xenopus
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity
Reactivity
top
Cdc2 phos Thr14/Tyr15
(poly)
IgG
Santa Cruz
Cat# sc-12340-R
immunogen = short aa seq. containing phosphorylated Thr14 and Tyr15 of human cdc2 p34
polyclonal rabbit
(liquid, PBS, gelatin, azide; blocking peptide sc-12340P)
IF, IP, WB
Cdc2 p34 phosphorylated at Thr14 and Tyr15 and correspondingly phosphorylated cdk2 and Cdk3
human, rat, mouse
Cdc2 phos Thr161
(poly)
Cell Signaling
Cat# 9114
immunogen = synthetic phospho-peptide (KLH-coupled) corresp. to residues surrounding Thr161 of human cdc2
polyclonal rabbit
(liquid, HEPES, NaCl, BSA, glycerol, protein A purified)
ELISA, IC, IF, IP, WB
detects endogenous levels of cdc2 only when phosphorylated at threonine 161; cross-reacts with endogenous CDK2 phosphorylated at threonine 160
human, rat, mouse
Cdc2 phos Thr161
(poly)
LifeSpan Biosciences, Inc.
Cat# LS-C5887
available in USA only
immunogen = synthetic phospho-peptide corresp. to residues surrounding Thr161 of human cdc2 (KLH coupled)
polyclonal rabbit
(protein A purified)
ELISA, IC, IF, IP, WB
endogenous levels of cdc2 phosphorylated at Thr161; cross-reacts with endogenous cdk2 phosphorylated at Thr160.
rat, mouse
Cdc2 phos Thr161
(poly)
IgG
Santa Cruz
Cat# sc-12341
Cat# sc-12341-R (rabbit)
immunogen = short aa seq. containing phosphorylated Thr161 of human cdc2 p34
polyclonal goat
(liquid, PBS, gelatin, azide; blocking peptide sc-12341P)
IF, WB
Cdc2 p34 phosphorylated at Thr161
human, rat, mouse
cdc2 phos Tyr15
(poly)
IgG
AnaSpec
Cat# 54332
immunogen = synthetic phospho-peptide corresp. to human CDC2 with phosphorylation site tyrosine 15 (EGT-pY-GV)
polyclonal rabbit
(liquid, affinity purified, PBS, azide)
DB
detects cdc2
human
Cdc2 phos Tyr15
(10A11)
IgG
Cell Signaling
Cat# 4539
immunogen = synthetic phosphopeptide (KLH-coupled) corresp. to residues surrounding Tyr15 of human cdc2
monoclonal rabbit
(liquid, HEPES, NaCl, BSA, glycerol, azide, protein A purified)
FC, IC, IF, IP, WB
endogenous levels of total cdc2 protein only when phosphorylated at Tyr15
monkey, human, rat, mouse
Cdc2 phos Tyr15
(poly)
Cell Signaling
Cat# 9111
immunogen = synthetic phosphopeptide (KLH-coupled) corresp. to residues surrounding Tyr15 of human cdc2
polyclonal rabbit
(liquid, HEPES, NaCl, BSA, glycerol, azide, protein A purified)
ELISA, IP, WB
detects endogenous levels of cdc2, CDK2 and CDK5 only when phosphorylated at tyrosine 15;
no
cross-reaction with CDK4, CDK6 or CDK7. Detects the yeast orthologue of cdc2 (cdc28) when phosphorylated at tyrosine 19
monkey, human, rat, mouse, drosophila, xenopus
Cdc2 phos Tyr15
(kit)
Cell Signaling
Cat# 9110
immunogen = synthetic peptide (KLH coupled) corresp.to residues surrounding the phsphorylation site
rabbit
(phosphoPlus® antibody kit)
ELISA, IP, WB
analysis of the phosphorylation status of cdc2 (Tyr15)
monkey, human, rat, mouse, drosophila, xenopus
cdc2 phos Tyr15
(poly)
Ig
Chemicon and Upstate are Millipore
Cat# AB3241
immunogen = synthetic peptide corresp. to aa surrounding the phosphoTry15 of human, rat, mouse and Xenopus CDC2
polyclonal rabbit
(liquid, HEPES, NaCl, BSA, glycerol, affinity purified)
WB
CDC2, phosphoTyr15 ~35kDa (labeling of CDC2 in WB of rat brain is blocked by the phosphopeptide but not by the dephosphopeptide
rat
(other species not tested)
cdc2 phos Tyr15
(poly)
Ig
Chemicon and Upstate are Millipore
Cat# AB3241-25μl
immunogen = synthetic peptide corresp. to aa surrounding the phospho Try15 of human, rat, mouse and Xenopus CDC2
polyclonal rabbit
(liquid, HEPES, NaCl, BSA, glycerol, affinity purified)
WB
CDC2, phosphoTyr15 ~35kDa (labeling of CDC2 in WB of rat brain is blocked by the phosphopeptide but not by the dephosphopeptide
rat
(other species not tested)
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity
Reactivity
top
Cdc2 phos Tyr15
(mono)
LifeSpan Biosciences, Inc.
Cat# LS-C22013
available in USA only
immunogen = synthetic peptide corresp. to residues surrounding Tyr15 of human Cdc2
monoclonal rabbit
(supernatant)
IC, IH, IP, WB
cdc2 phosphorylated at Tyr15
human
Cdc2 phos Tyr15
(poly)
LifeSpan Biosciences, Inc.
Cat# LS-C5888
available in USA only
immunogen = synthetic phospho-peptide corresp. to residues around Tyr15 of human cdc2, KLH coupled
polyclonal rabbit
(protein A purified)
IC, IF, IH, IP, WB
detects endogenous levels of cdc2, cdk2 and cdk5 only when catalytically inactivated by phosphorylation at Tyr15;
no
cross-reactivity with cdk4, cdk6 or cdk7
monkey, human, rat, mouse, xenopus
Cdc2 phos Tyr15
(poly)
LifeSpan Biosciences, Inc.
Cat# LS-C5889
available in USA only
immunogen = synthetic phospho-peptide corresp. to aa residues surrounding the phosphorylated Tyr15 of rat, human, mouse and Xenopus p34(Cdc2)
polyclonal rabbit
(protein A purified)
WB
p34 (cdc2) when phosphorylated on Tyr15
human, rat, mouse, xenopus
Cdc2 phos Tyr15
(poly)
LifeSpan Biosciences, Inc.
Cat# LS-C5891
available in USA only
immunogen = synthetic phospho-peptide corresp. to aa residues surrounding the phosphorylated Tyr15 of rat, human, mouse and Xenopus p34(Cdc2)
polyclonal rabbit
(DEAE-column chromatography)
WB
cdc2 when phosphorylated on Tyr15 (~35kDa)
human, rat, mouse, xenopus
Cdc2 phos Tyr15
(poly)
LifeSpan Biosciences, Inc.
Cat# LS-C5894
available in USA only
immunogen = synthetic phospho-peptide corresp. to aa residues surrounding the phosphorylated Tyr15 of rat, human, mouse and Xenopus p34(Cdc2)
polyclonal rabbit
(protein A purified)
DB, WB
cdc2 when phosphorylated on Tyr15 (~35kDa)
human, rat, mouse, drosophila, xenopus
Cdc2 phos Tyr15
(poly)
Phospho- solutions
Cat# p135-15
immunogen = phospho peptide corresp. to aa residues surrounding the phospho Tyr15 of rat cdc2
polyclonal rabbit
(serum, affinity purified)
WB
Specific for the ~38k cdc2 protein phosphor. at Tyr15
human, xenopus
(expect mouse, rat and zebra fish)
Cdc2 phos Tyr15
(poly)
IgG
Santa Cruz
Cat# sc-7989
Cat# sc-7989-R (rabbit)
immunogen = epitope mapping at the C-terminus of Cdc2 p34 of human origin
polyclonal goat
(liquid, PBS, gelatin, azide, affinity purified; blocking peptide sc-7989P)
IF, IP, WB
Cdc2 phosphorylated at Tyr15 and correspondingly phosphorylated Cdk2 and Cdk3
human, rat, mouse, cow, various species
cdc2 phos Y15
(poly)
IgG
Abcam
Cat# ab10533
immunogen = synthetic peptide (human) conjugated to KLH
polyclonal rabbit
(liquid, HEPES, NaCl, BSA, glycerol, no preservative, affinity purified)
WB
detects cdc2 and cdk2 only when catalytically inactivated by phosphorylation at Tyr15;
no
reaction with non-phosphorylated cdc2, nor cdk4, cdk6 or cdk7
human, mouse
Cdc2 phosThr14/Tyr15
(poly)
LifeSpan Biosciences, Inc.
Cat# LS-C5954
available in USA only
immunogen = synthetic phospho-peptide derived from the region of the human cyclin-dependent kinases that contain threonine 14 and tyrosine 15
polyclonal rabbit
(unpurified serum)
ELISA, IP, WB
cdk1; may recognize other cyclin-dependent kinases phosphorylated on the homologous residues;
no
reactivity to cdk4 or cdk6.
human, rat, mouse
Cdc2 phosTyr15
(poly)
IgG
AbD Serotec
Cat# AHP895
immunogen = synthetic phosphopeptide corresp. to an aa seq. within CDC2 which includes phosphorylated Tyr15
polyclonal rabbit
(liquid, HEPES, BSA, glycerol, azide, affinity purified)
WB
cdc2 phosphorylated at Tyr15 (34kDa)
human, xenopus
(expect rat, mouse, zebrafish)
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity
Reactivity
top
Cdc2 pTyr15
(poly)
Novus Biologicals
Cat# NB 500-142
immunogen = phosphopeptide corresp. to aa residues surrounding the phospho-Tyr15 of rat cdc2.
polyclonal rabbit
(liquid, serum, HEPES, NaCl, BSA, glycerol, affinity purified)
WB
CDC2 protein, phosphorylated at Tyr15 (34kDa)
human, rat, mouse, drosophila, xenopus
Cdc2 Tyr15
(poly)
LifeSpan Biosciences, Inc.
Cat# LS-C5892
available in USA only
immunogen = synthetic phospho-peptide corresp. to an aa seq. within CDC2 which includes phosphorylated Tyr 15
polyclonal rabbit
(protein G purified)
IH(F)
, WB
cdc2 phosphorylated at Tyr15
human
Cdc2B
(poly)
IgG
Santa Cruz
Cat# sc-12830
epitope = mapping near the C-terminus of Cdc2B of
Arabidopsis thaliana
origin
polyclonal goat
(liquid, PBS, gelatin, azide; blocking peptide sc-12830P)
IF, WB
Cdc2B
Arabidopsis thaliana
Cdc2-CRK7
(poly)
Novus Biologicals
Cat# NLS 7350
immunogen = synthetic peptide made to the internal region of cdc2-CRK7
polyclonal rabbit
(liquid, serum, PBS, azide)
IH(P)
CDC2-related protein kinase 7 (CRK7)
human
(other species not tested)
Cdc2L5
(1B3-1B11)
IgG
1
, κ
Novus Biologicals
Cat# H00008621-M01
supplied by Abnova
available in N. America only
immunogen = CDC2L5 (AAH01274, 1 a.a. ~ 325 a.a) full length recombinant protein with GST tag
monoclonal mouse
(liquid, purified, no preservative)
WB
CDC2L5 - cell division cycle 2-like 5 (cholinesterase-related cell division controller)
human
(other species not tested)
Cdc2L5
(3G1)
IgG
2a
, κ
Novus Biologicals
Cat# H00008621-M02
supplied by Abnova
available in N. America only
immunogen = paartial recombinant CDC2L5
monoclonal mouse
(liquid, purified, no preservative)
WB
CDC2L5 - cell division cycle 2-like 5 (cholinesterase-related cell division controller)
human
(other species not tested)
CDK phos Ser
(kit)
Cell Signaling
Cat# 2324
immunogen = synthetic phospho-CDK substrate peptides
rabbit
(liquid, HEPES, NaCl, BSA, glycerol, protein A purified)
ELISA,
IH(P)
, IP, WB;
DELFIA
detects phospho-serine in a (K/R)(S*)PX(K/R) motif; does
not
recognize phospho-serine in the absence of the CDK motif.
No
cross-reaction with phospho-threonine- or phospho-tyrosine-containing peptides/proteins
monkey, human, rat, mouse, bovine, drosophila, xenopus
Cdk1
(A17.1.1)
IgG
2a
Chemicon and Upstate are Millipore
Cat# MAB8878
immunogen = Xenopus p34cdc2
monoclonal mouse
(liquid, PBS, BSA, azide, purified)
IF,
IH(P)
, IP, WB; kinase assay
recognizes cdk1 (34 kDa) (cdk1/p34cdc2 protein kinase, a catalytic subunit of maturation promoting factor); epitope maps near the C-terminus of protein and its core is thought to be L G T P N N E V; shows
no
cross-reaction with cdk2/p32 and other cyclin dependent kinases (cdks)
human, rat, mouse, xenopus, chicken
(other species not tested)
CDK1 + CDK2
(AN21.2)
IgG
2a
Abcam
Cat# ab6434
immunogen = cdk2
monoclonal mouse
(liquid, PBS, protein A purified)
ELISA, WB
recognises both cdk1 and cdk2 - useful as a general cdk antibody since it reacts with both cdk1 and cdk2 and is very sensitive giving an intense signal
human
Cdk1/cdc2
(DW3)
IgG
Upstate and Chemicon are Millipore
Cat# 05-951
Discontinued
immunogen = His-tagged, full-length recombinant human cdk1/cdc2
monoclonal rabbit
(liquid, tris-glycine saline, glycerol, azide, protein A purified)
IP, WB
cdk1/cdc2 kinase
human, mammal (various)
(other species not tested)
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