|
Antibodies to α-, β-, γ-Secretase Proteins
|
| From the Laboratories
|
Antigen
(Clone)
IgG |
Name
(Source)
used in |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
β-secretase
(poly)
|
7523
(C. Haass
Paper)
Reference:
Rajendran, 2006 |
|
polyclonal rabbit
|
IC, IP, WB |
N terminus (amino acids 46-60) |
human |
BACE1
(3D5)
IgG1
|
BACE-Cat1
Reference:
O'Connor, 2008 |
immunogen = N terminus catalytic domain (amino acids 46–460) with N-terminal 6xHis tag |
monoclonal mouse
(affinity purified) |
ELISA, IB, IF, IH |
recognizes the
catalytic domain of BACE-1.
Paper by Zhao et al., 2007 |
human, mouse |
BACE1
(9B21)
|
Ab9B21
(SmithKline Beecham Pharmaceuticals, Harlow, UK)
Reference:
Parkin, 2007 |
immunogen = BACE1-Fc fusion protein |
monoclonal mouse
|
IB |
recognizes the
catalytic domain of BACE1; allows detection of soluble shed BACE1 |
human |
memapsin 2 /BACE1
(poly)
|
anti-M2
Reference:
Chang, 2007 |
immunogen = memapsin 2 ectodomain (Paper) |
polyclonal goat
(liquid, affinity purified, Alexa 488 conj.) |
Block, IF, IS |
Memapsin 2 ectodomain; some cross with memapsin 1 (BACE-2) |
mouse |
| From the Manufacturers
|
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
α-Secretase
(kit)
|
R&D Systems
Cat #
FP001
Reference:
Wang, 2007 |
immunogen = APP
peptide Y E V H H Q K L V using E D A N S D A B C Y L as the reporter system |
(extraction buffer, reaction buffer, 96 well microplate) |
IF |
detects enzymatic activity of the α-secretase class of proteases associated with
the cleavage of APP |
|
β-Secretase
(kit)
|
R&D Systems
Cat #
FP002
Reference:
Wang, 2007 |
immunogen = APP
peptide REEVNLDAEFKR using EDANS/DABCYL as the reporter system |
(extraction buffer, reaction buffer, 96 well microplate) |
IF |
detects enzymatic activity of the β-secretase class of proteases associated with
the cleavage of APP |
|
γ-Secretase
(kit)
|
R&D Systems
Cat #
FP003
Reference:
Wang, 2007 |
immunogen = APP
peptide GVVIATVIV using EDANS/DABCYL as the reporter system |
(extraction buffer, reaction buffer, 96 well microplate) |
IF |
detects enzymatic activity of the γ-secretase class of proteases associated with
the cleavage of APP |
|
BACE
(poly)
IgG
|
Calbiochem EMD Biosciences
Cat# PC529
Discontinued |
immunogen = synthetic peptide corresp. to aa 485-501 (C L R Q Q H D D F A D D I S L L K) of human BACE |
polyclonal rabbit
(liquid, PBS, azide, purified by ion exchange chromatography) |
WB |
70 kDa BACE |
human, mouse |
BACE
(poly)
IgG
|
Calbiochem EMD Biosciences
Cat# 195100 |
immunogen = a synthetic peptide, C-I R L P L R S G L G G A P L G L R L P R, corresp. to aa 26-45 of human BACE |
polyclonal rabbit
(liquid, serum, no preserve) |
IP, WB |
recognizes the ~65 and ~75 kDa glycosylated froms of the BACE protein |
human |
BACE
(poly)
IgG
|
Calbiochem EMD Biosciences
Cat# 195101 |
immunogen = synthetic peptide, E T D E E P E E P G R R G S F V E M V D-C, corresp. to aa 46-65 of human BACE |
polyclonal rabbit
(liquid, serum, no preserve) |
IP, WB |
recognizes the ~65 and ~75 kDa glycosylated from of BACE protein |
human |
BACE
(poly)
IgG
|
Calbiochem EMD Biosciences
Cat# 195102 |
immunogen = synthetic peptide, C-R Q Q H D D F A D D I S L L K, corresp. to aa 487-501 of human BACE |
polyclonal rabbit
(liquid, serum, no preserve) |
IP, WB |
recognizes the ~65 and ~75 kDa glycosylated forms of BACE protein |
human, hamster |
BACE
(kit)
|
Calbiochem EMD Biosciences
Cat#565785 |
|
|
IF |
BACE activity |
(biological and purified samples) |
BACE
(61-3E7)
IgG1
|
Chemicon and Upstate are Millipore
Cat# MAB5308
references:
Li, 2003
Fukumoto, 2002
Lee, 2002 |
immunogen = synthetic peptide, C-terminus of BACE |
monoclonal mouse
(purified, PBS, azide) |
IC(C), IH(P), IP, WB |
BACE, not BACE2; recognizes pro and mature forms |
human, primate (various), rat, mouse
(other species not tested) |
BACE
(poly)
|
Chemicon and Upstate are Millipore
Cat# AB5940 |
immunogen = synthetic peptide corresponding to C-terminus of human BACE |
polyclonal rabbit
(liquid, serum, affinity purified, PBS, azide) |
IH, WB |
BACE; recognizes a band of ~70-75 kDa. and at ~45 kDa |
monkey, human, rat, mouse
(other species not tested) |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
BACE
(poly)
IgG
|
Chemicon and Upstate are Millipore
Cat# AB5488 |
immunogen = synthetic peptide corresponding to aa 485-501 of BACE |
polyclonal goat
(serum, thimerosal) |
ELISA, IH, WB |
70 kDa glycosylated form of BACE. |
human |
BACE
(poly)
Ig
|
Chemicon and Upstate are Millipore
Cat# AB5832 |
immunogen = synthetic peptide corr. to aa 458-501 of BACE |
polyclonal rabbit
(purified, PBS, azide) |
WB |
recognizes BACE |
human, mouse |
BACE
(poly)
IgG
|
Exalpha Biologicals
Cat# X1135P |
synthetic peptide, aa485-501 of human BACE, C-terminal |
polyclonal rabbit
(purified, PBS, azide) |
WB |
70 kDa BACE |
human, mouse |
BACE
(poly)
IgG
|
Novus Biologicals
cat# NB120-10716 |
immunogen = synthetic peptide, C L R Q Q H D D F A D D I S L L K, aa 458-501 of human BACE |
polyclonal rabbit
(liquid, affinity purified, BSA, PBS, azide) |
WB |
Transfected BACE1 |
human
(expect mouse, rat) |
BACE
(poly)
IgG
|
Novus Biologicals
cat# NB120-2077 |
immunogen = Synthetic peptide, corresp. to aa. 485-501 of Human BACE; peptide sequence differs from those of mouse and rat BACE by one amino acid |
polyclonal rabbit
(liquid antisera, PBS, azide, purified) |
WB |
detects the 70 kD band of BACE |
human, rat, mouse
(others not tested) |
BACE
(poly)
IgG
|
ProSci
Cat# 2253
Reference:
Rockenstein, 2007 |
immunogen = aa 485 to 501, (C L R Q Q H D D F A D D I S L L K) of human BACE |
polyclonal rabbit
(purified, azide, Store at -20°) |
WB |
70 kDa BACE |
human, mouse |
BACE
(poly)
IgG
|
QED Bioscience
Cat# 2253 |
immunogen = synthetic peptide corresp. to aa. 485-501 near the C-terminal of human BACE |
polyclonal rabbit
(liquid, purified, PBS, azide) |
IB |
~70 kDa BACE protein |
human, mouse |
BACE
(poly)
|
AbD Serotec
Cat # AHP-740 |
immunogen = synthetic peptide, C L R Q Q H D D F A D D I S L L K, corresp.to the C-terminal aa seq. 458-501 of human BACE |
polyclonal goat
(liquid, serum, purified, azide) |
IH(P) |
BACE, C-terminal |
human |
BACE (61-3E7)
(poly)
IgG1
|
Santa Cruz
Cat# sc-33711 |
immunogen = full length of human BACE |
polyclonal mouse
(purified, PBS, azide) |
IF, IH(P), IP, WB |
BACE |
human, rat, mouse |
BACE (ab-1)
(poly)
IgG
|
Calbiochem EMD Biosciences
Cat# PC478 |
immunogen = synthetic peptide corresp. to aa 44-59 of human BACE |
polyclonal rabbit
(liquid, antiserum) |
IH(P) |
BACE |
human |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
BACE (C-15)
(poly)
IgG
|
Santa Cruz
Cat# sc-10055 |
immunogen peptide mapping at the C-terminus of human BACE2 |
polyclonal goat
(purified, PBS, azide) |
IH, WB |
BACE |
human, rat, mouse |
BACE (D-16)
(poly)
IgG
|
Santa Cruz
Cat# sc-10053 |
immunogen = peptide mapping within and internal region of human BACE2 |
polyclonal goat
(purified, PBS, azide) |
IH, WB |
BACE |
human, rat, mouse |
BACE (M-83)
(poly)
IgG
|
Santa Cruz
Cat# sc-10748 |
immunogen = peptide mapping corr. to aa. 419-501 at the C-terminus of mouse BACE |
polyclonal rabbit
(purified, PBS, azide) |
IH, IP, WB |
BACE |
human, rat, mouse |
BACE (Z-183)
(mono)
IgG1
|
Santa Cruz
Cat# sc-73729 |
immunogen = raised against the aa22-460 of human BACE |
monoclonal mouse
(purified, PBS, azide) |
FC, IF, IH(P), WB |
BACE |
human, mouse |
BACE 1
(2C1)
IgGmix, κ
|
Novus Biologicals
cat# H00023621-M01
supplied by Abnova
available in North America only
|
immunogen = full length recombinant BACE1 |
monoclonal mouse
(liquid, purified, no preservative) |
WB |
BACE1 - beta-site APP-cleaving enzyme 1 |
human
(others not tested) |
BACE 1
(2C1)
IgG mix, κ
|
Novus Biologicals
cat# H00023621-M01
produced by Abnova |
immunogen = BACE1 (AAH65492, aa22-502 a.a) full length recombinant protein with GST tag |
monoclonal mouse
(liquid, no preservative, purified) |
ELISA, IH(P), WB |
BACE1 |
human |
BACE 1
(poly)
IgG
|
Novus Biologicals
cat# NB100-92016 |
immunogen = synthetic peptide corresp. to aa S28 |
polyclonal rabbit
(liquid, PBS, azide, affinity purified) |
IF, IH, WB |
detects endogenous levels of BACE protein |
human, mouse |
BACE 1
(poly)
IgG
|
Novus Biologicals
cat# NB100-94362 |
immunogen = raised against a peptide corresp. to aa485 to 501 of human BACE |
polyclonal rabbit
(liquid, PBS, azide) |
WB |
BACE 1 |
human, mouse |
BACE 1
(poly)
IgG
|
Novus Biologicals
cat# NB110-39056 |
immunogen = synthetic peptide corresp. to internal domain of human BACE1 |
polyclonal rabbit
(liquid, PBS, azide, affinity purified) |
IH(P) |
BACE1 |
human |
BACE 1
(poly)
IgG
|
Novus Biologicals
cat# NB110-39052 |
immunogen = synthetic peptide near N-terminus (aa30-80) of human |
polyclonal rabbit
(liquid, PBS, azide, affinity purified) |
IH(P) |
BACE1 |
human |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
BACE 1
(poly)
|
Novus Biologicals
cat# NB100-90596 |
immunogen = synthetic peptide near N-terminus (aa30-80) of human BACE |
polyclonal rabbit
(liquid, PBS, azide, affinity purified) |
IH(P) |
BACE1 |
human
(22% seq. homology with mouse and rat) |
BACE 1
(poly)
|
Novus Biologicals
cat# H00023621-D01P
produced by Abnova |
immunogen = BACE1 (NP_620428.1, aa1-476) full-length human protein |
polyclonal rabbit
(liquid, no preservative, protein A purified) |
ELISA, WB |
BACE1 |
human |
BACE 1
(poly)
|
Novus Biologicals
cat# NB100-65622 |
immunogen = synthetic peptide C L R Q Q H D D F A D D I S L L K corresp. to the C-terminal aa seq. 458-501 of human BACE |
polyclonal goat
(liquid, whole antisera, azide) |
IH(P) |
BACE1 |
human |
BACE 1
(poly)
IgG
|
Novus Biologicals
cat# NB100-92016 |
immunogen = peptide made to the amino acid region S28 |
polyclonal rabbit
(liquid, PBS, azide, affinity purified) |
ELISA, IF, IH |
endogenous levels of BACE1 |
human, mouse |
BACE 1
(poly)
IgG
|
Novus Biologicals
cat# NB120-2077 |
immunogen = synthetic peptide, corresp. to aa485-501 of human BACE |
polyclonal rabbit
(liquid, PBS, azide, purified) |
WB |
endogenous levels of BACE1 |
human, rat, mouse |
BACE 1 phos Ser498
(poly)
IgG
|
Novus Biologicals
cat# PAB0414 |
immunogen = synthetic peptide corresp. to residues surrounding Ser498 of human BACE1 |
polyclonal rabbit
(liquid, whole antiserum, azide) |
ELISA, IH(P), WB |
BACE1-beta-site APP-cleaving enzyme 1 [Ser498] |
human |
BACE 2
(poly)
IgG
|
Novus Biologicals
cat# NB120-8025 |
immunogen = synthetic peptide corresponding to aa near the N-terminus of human BACE 2 |
polyclonal rabbit
(liquid, PBS, azide, purified) |
IH, WB |
BACE2 |
human, rat, mouse |
BACE 2
(poly)
IgG
|
Novus Biologicals
cat# NBP1-03211 |
immunogen = synthetic peptide corresp. to aa44-59 of human BACE2 |
polyclonal rabbit
(liquid, PBS, azide, affinity purified) |
IH(P), WB |
BACE2 |
human, rat, mouse |
BACE 2
(poly)
IgG
|
Novus Biologicals
cat# NB100-94360 |
immunogen = raised against a synthetic peptide (A P T P G P G T P A E R H A D G) corresp. to aa44 to 59 of human BACE2 |
polyclonal rabbit
(liquid, PBS, azide) |
WB |
BACE /Asp2 |
human, rat, mouse |
BACE 2
(poly)
IgG
|
Novus Biologicals
cat# NB100-66046 |
immunogen = synthetic peptide corresp. to aa44-59 of human BACE2 |
polyclonal rabbit
(liquid, PBS, whole antiserum, azide) |
WB |
recognizes an epitope within the N-terminal region of BACE2, a homologue of the beta-site APP cleaving enzyme (BACE) |
human, rat, mouse |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
BACE 2
(poly)
IgG
|
Novus Biologicals
cat# NB100-94361 |
immunogen = raised against a peptide corresp. to aa496 to 511 of human BACE |
polyclonal rabbit
(liquid, PBS, azide) |
WB |
BACE /Asp2 |
human |
BACE 2
(poly)
IgG
|
Novus Biologicals
cat# NB120-5670 |
immunogen = synthetic peptide: V S E I S G P F S T E D I A S N C, corresp. to aa441-457 of human BACE2 |
polyclonal rabbit
(liquid, PBS, azide, affinity purified) |
WB |
BACE 2 |
human, rat, mouse |
BACE 2
(poly)
IgG
|
Novus Biologicals
cat# NB120-5671 |
immunogen = synthetic peptide: C P E V V N D E S S LV R H R W K, corresp. to aa503-518 of human BACE2 |
polyclonal rabbit
(liquid, PBS, azide, affinity purified) |
WB |
BACE 2 |
human |
BACE 2
(poly)
IgG
|
Novus Biologicals
cat# NB120-8025 |
immunogen = synthetic peptide corresp. to aa near the N-terminus of human BACE 2 |
polyclonal rabbit
(liquid, PBS, azide, purified) |
IH, WB |
BACE /Asp2 (70 kDa) |
human, rat, mouse |
BACE 2
(poly)
IgG
|
Novus Biologicals
cat# NBP1-03211 |
immunogen = synthetic peptide corresp. to aa44-59 of human BACE2 |
polyclonal rabbit
(liquid, PBS, azide, affinity purified) |
IH(P), WB |
BACE /Asp2 (aa44-59) |
human, rat, mouse |
BACE 2
(poly)
IgG
|
Novus Biologicals
cat# NB100-94361 |
immunogen = peptide corresp. to aa496-511 of human BACE2 |
polyclonal rabbit
(liquid, PBS, azide, purified) |
WB |
BACE /Asp2 |
human |
BACE, CT
(poly)
IgG
|
AnaSpec
Cat# 54101 |
immunogen = a synthetic peptide corresponding to 17 amino acids at the C-terminus of human BACE |
polyclonal rabbit
(liquid, ion exchange purified, PBS, azide) |
IC, WB |
detects BACE |
human, mouse |
BACE/Asp2
(poly)
|
A D I
Cat# BACE11-A
Cat# BACE11-S |
immunogen = a 17 aa peptide, designated BACE11-P control peptides, near the CT of human BACE/Asp2, KLH conj. |
polyclonal rabbit
(liquid, serum or affinity purified) |
ELISA, WB |
BACE/Asp2 only, not BACE2/Asp1 |
rat, mouse |
BACE/Asp2
(poly)
|
A D I
Cat# BACE12-A
Cat# BACE12-S |
immunogen = a 10 aa peptide, designated BACE12-P control peptide, near the extracellular NT of mouse BACE/Asp2, KLH conj. |
polyclonal rabbit
(serum, or affinity purified) |
ELISA, WB |
BACE/Asp2 only, not BACE2/Asp1 |
human, rat |
BACE1
(poly)
IgG
|
Abcam
cat# ab10716 |
immunogen = synthetic peptide, C L R Q Q H D D F A D D I S L L K, aa 458-501 of human BACE |
polyclonal rabbit
(liquid, affinity purified, BSA, PBS, azide) |
WB |
Transfected BACE1 |
human
(expect mouse, rat) |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
BACE1
(poly)
IgG
|
Abcam
cat# ab11028 |
immunogen = synthetic peptide, C L R Q Q H D D F A D D I S L L K, aa 458-501 of human BACE |
polyclonal rabbit
(liquid, whole serum, azide) |
IH(P) |
70 kDa BACE, plasma membrane |
human
(expect mouse, rat) |
BACE1
(poly)
IgG
|
Abcam
cat# ab2077
Reference:
Xie, 2007 |
immunogen = synthetic peptide, corresp. to aa 485-501 of human BACE |
polyclonal rabbit
(liquid, PBS, azide, puriafied) |
WB |
BACE |
human, rat, mouse |
BACE1
(poly)
IgG
|
Abcam
cat# ab23796 |
immunogen = recombinant full length protein human BACE: solubilized total recombinant protein |
polyclonal rabbit
(liquid, PBS, BSA, protein A purified) |
WB |
BACE1; cross-reactivity with BACE2 is almost negligible |
human
(others not tested) |
BACE1
(poly)
IgG
|
Abcam
cat# ab23800 |
immunogen = synthetic peptide (I R L P L R S G L G G A P L G L R L P R) corresp. to aa 26-45 of human BACE1 |
polyclonal rabbit
(liquid, whole serum, azide) |
WB |
proBACE; not mature BACE |
human
(expect mouse, rat) |
BACE1
(poly)
IgG
|
Abcam
cat# ab23801
Discontinued |
immunogen = synthetic peptide (E T D E E P E E P G R R G S F V E M V D) corresp. to aa 46-65 of human BACE1 |
polyclonal rabbit
(liquid, whole serum, azide) |
WB |
proBACE and mature BACE |
human
(expect mouse, rat) |
BACE1
(poly)
IgG
|
Abcam
cat# ab23802
Discontinued |
immunogen = synthetic peptide (R Q Q H D D F A D D I S L L K) corresp. to aa 487-501 of human BACE1 |
polyclonal rabbit
(liquid, whole serum, azide) |
WB |
proBACE and mature BACE |
human
(expect mouse, rat, dog) |
BACE1
(poly)
IgG
|
Abcam
cat# ab62034 |
immunogen = synthetic peptide from the N- terminus of human BACE1, conjugated to KLH |
polyclonal rabbit
(liquid, PBS, azide, affinity purified) |
IH(P) |
BACE |
human, rat, mouse |
BACE1
(poly)
IgG
|
Abcam
cat# ab62048 |
immunogen = synthetic peptide from an internal seq. of human BACE1, conj. to KLH |
polyclonal rabbit
(liquid, PBS, azide, affinity purified) |
IH(P) |
BACE1 |
human |
BACE1
(poly)
IgG
|
Abcam
cat# ab10716 |
immunogen = synthetic peptide, C L R Q Q H D D F A D D I S L L K, aa 458-501 of human BACE |
polyclonal rabbit
(liquid, affinity purified, BSA, PBS, azide) |
WB |
Transfected BACE1 |
human
(expect mouse, rat) |
BACE1
(poly)
|
Biomol Enzo Life Sciences
cat# SA-465 |
immunogen = synthetic peptide based on the catalytic domain of the human BACE1 seq. |
polyclonal rabbit
(liquid, PBS, glycerol, azide, affinity purified) |
IB |
recognizes zymogen and mature BACE1; no cross with BACE2 |
human, rat, mouse
(others not tested) |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
BACE1
(poly)
IgG
|
Biosource Invitrogen
Cat# AHB0241 |
immunogen = solubilized total recombinant protein |
polyclonal rabbit
(affinity purified, PBS, BSA, azide) |
WB |
BACE1, 70 kDa |
human
(others not tested) |
BACE1
(poly)
|
Biosource Invitrogen
cat# AHB0291
discontinued |
immunogen = synthetic peptide, corr. to C-terminal 15 residues of human β-secretase 1 |
polyclonal rabbit
(processed whole serum, azide) |
WB |
proBACE, and mature BACE |
human
(others not tested) |
BACE1
(GP45)
Ig
|
Covance Research Products, Inc.
Cat# PGP 624C |
immunogen = a peptide sequence C L R Q Q H D D F A D D I S L L K , the C-terminal 17 aa of human BACE1 |
monoclonal guinea pig
(crude antiserum) |
IH, IP, WB |
recognises the mature and immature forms of BACE |
human, rat, mouse |
BACE1
(poly)
|
I B L Company, Ltd.
Cat# 18711 |
immunogen = synthetic peptide of C-terminal of human BACE1, (C L R Q Q H D D F A D D I S L L K) |
polyclonal rabbit
(lyophilized, affinity purified, PBS, BSA, azide) |
IH(P), IP, WB |
BACE 1 |
human, rat, mouse |
BACE1
(kit)
|
I B L Company, Ltd.
Cat# 27752 |
|
|
ELISA |
BACE 1 |
human, rat, mouse |
BACE1
(poly)
|
LifeSpan Biosciences, Inc.
cat# LS-A8893 |
immunogen = synthetic peptide of internal domain of human BACE1, conj. KLH |
polyclonal rabbit
(liquid, PBS, azide) |
IH(P) |
BACE 1 |
human |
BACE1
(poly)
|
LifeSpan Biosciences, Inc.
cat# LS-A8548 |
immunogen = synthetic peptide near the N-terminus of human BACE1, conj. KLH |
polyclonal rabbit
(liquid, affinity purified) |
IH |
BACE 1 |
human
(mouse has 94% identity/similarity; rat has 100% identity/similarity) |
BACE1
(poly)
|
LifeSpan Biosciences, Inc.
cat# LS-C12062
available in USA only |
immunogen = synthetic peptide corresp. to 10 aa near the NT of mouse BACE/Asp2 conjugated to KLH |
polyclonal rabbit
(DEAE-column chromatography) |
ELISA, IF, IH, WB |
BACE 1 |
mouse
(human has 100% homology and rat has 90% homology) |
BACE1
(poly)
|
LifeSpan Biosciences, Inc.
cat# LS-C377 |
immunogen = synthetic peptide corresp. to 17 aa near the CT of human BACE |
polyclonal rabbit
(liquid, PBS, azide, ion exchange chromatography) |
IC, WB |
BACE 1 |
human, mouse |
BACE1
(poly)
|
LifeSpan Biosciences, Inc.
cat# LS-C11849
available in USA only |
immunogen = synthetic peptide (C L R Q Q H D D F A D D I S L L K) |
polyclonal goat
(unpurified serum) |
ELISA, IH |
recognizes the C-terminal aa sequence 485-501 of human beta amyloid cleaving enzyme protein
|
human |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
BACE1
(poly)
|
LifeSpan Biosciences, Inc.
cat# LS-C12061
available in USA only |
immunogen = synthetic peptide 17 aa within the cytoplasmic, C-terminal domain of the rat NHE4, conj. KLH |
polyclonal goat
(unpurified serum) |
ELISA, WB |
BACE1
|
human |
BACE1
(poly)
|
LifeSpan Biosciences, Inc.
cat# LS-C12063
available in USA only |
immunogen = synthetic peptide 10 aa peptide near the NT of mouse BACE/Asp2 |
polyclonal goat
(unpurified serum) |
ELISA, WB |
BACE1
|
human
(expect mouse and rat) |
BACE1
(poly)
|
LifeSpan Biosciences, Inc.
cat# LS-C12065
available in USA only |
immunogen = synthetic peptide 10 aa peptide near the NT of mouse BACE/Asp2, conj. to KLH |
polyclonal rabbit
(DEAE-column chromatography) |
ELISA, WB |
BACE/Asp2
|
mouse
(expect human and rat due to seq. homology) |
BACE1
(poly)
|
LifeSpan Biosciences, Inc.
cat# LS-C12066
available in USA only |
immunogen = synthetic peptide 17 aa peptide near the CT of human BACE/Asp2, conj. to KLH |
polyclonal rabbit
(DEAE-column chromatography) |
ELISA, WB |
BACE/Asp2
|
human
(expect mouse and rat due to seq. homology) |
BACE1
(poly)
|
LifeSpan Biosciences, Inc.
cat# LS-C2951 |
immunogen = synthetic peptide conjugated to KLH |
polyclonal rabbit
|
ELISA |
BACE 1 |
human |
BACE1
(poly)
|
LifeSpan Biosciences, Inc.
cat# LS-C2952 |
immunogen = synthetic peptide conjugated to KLH |
polyclonal rabbit
|
ELISA |
BACE 1 |
human |
BACE1
(poly)
|
LifeSpan Biosciences, Inc.
cat# LS-C2954 |
immunogen = synthetic peptide conjugated to KLH |
polyclonal rabbit
|
ELISA |
BACE 1 |
human |
BACE1
(poly)
|
LifeSpan Biosciences, Inc.
cat# LS-C3876
available in USA only |
immunogen = solubilized total recombinant protein |
polyclonal rabbit
(protein A purified) |
WB |
BACE 1; cross-reactivity with BACE2 is almost negligible |
human |
BACE1
(poly)
|
LifeSpan Biosciences, Inc.
cat# LS-C3877
available in USA only |
immunogen = synthetic peptide corresp. to aa sequence 26-45 of BACE1 |
polyclonal rabbit
(unpurified serum) |
WB |
BACE 1; proBACE; does not detect mature BACE |
human |
BACE1
(poly)
|
LifeSpan Biosciences, Inc.
cat# LS-C12067
available in USA only |
immunogen = synthetic peptide C L R Q Q H D D F A D D I S L L K corresp. to the C-terminal aa458-501 of human BACE |
polyclonal goat
(unpurified serum) |
IH(P) |
recognizes beta amyloid cleaving enzyme (BACE), a 70kD aspartic protease |
human |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
BACE1
(poly)
|
LifeSpan Biosciences, Inc.
cat# LS-C12068
available in USA only |
immunogen = synthetic peptide derived from the C-terminal region of the human BACE protein |
polyclonal rabbit
(DEAE-column chromatography) |
WB |
recognizes C-terminal of BACE protein |
mouse
(expect human and rat due to seq. homology) |
BACE1
(poly)
|
LifeSpan Biosciences, Inc.
cat# LS-C17788
available in USA only |
immunogen = synthetic peptide corresponding to aa 485-501 (C485 L R Q Q H D D) |
polyclonal rabbit
(DEAE-column chromatography) |
WB |
recognizes secretase. (~70kDa) |
human |
BACE1
(poly)
|
LifeSpan Biosciences, Inc.
cat# LS-C11526 |
|
polyclonal goat
|
WB |
BACE1 |
human |
BACE1
(poly)
|
LifeSpan Biosciences, Inc.
cat# LS-C3878
available in USA only |
immunogen = synthetic peptide corresp. to aa46-65 (E T D E E P E E P G R R G S F V E M V D) of human BACE1 |
polyclonal rabbit
(unpurified serum) |
WB |
proBACE and mature BACE |
human |
BACE1
(poly)
|
LifeSpan Biosciences, Inc.
cat# LS-A8549 |
immunogen = synthetic peptide near the N-terminus of human BACE1, conj. KLH |
polyclonal rabbit
(liquid, PBS, azide, affinity purified) |
IH(P) |
BACE 1 |
human
(mouse and rat have 22% identity and 44% similarity) |
BACE1
(poly)
IgG
|
LifeSpan Biosciences, Inc.
cat# LS-C42439 |
immunogen = synthetic peptide near the N-terminal of human BACE1 |
polyclonal rabbit
(liquid, PBS) |
IH(P) |
BACE 1 |
human |
BACE1
(poly)
|
LifeSpan Biosciences, Inc.
cat# LS-C26627 |
|
polyclonal rabbit
|
WB |
BACE 1 |
|
BACE1
(poly)
IgG
|
AbD Serotec
Cat # AHP-1119 |
immunogen = synthetic peptide near N-terminal of human BACE1 |
polyclonal rat
(liquid, Ig, purified, PBS, azide) |
IH(P) |
BACE1 |
human, rat, mouse |
BACE1
(poly)
|
Zymed/ Invitrogen
Cat# 34-4900 |
immunogen = synthetic peptide derived from the C-terminal region of human BACE
|
polyclonal rabbit
(affinity purified, PBS, azide) |
WB |
C-terminal region of mouse BACE |
mouse
(expect rat and human; others not tested) |
BACE-1
(137612)
IgG1
|
R&D Systems
Cat #
IC-931F |
|
monoclonal mouse
(liquid, PBS, azide, CFS conj.) |
FC |
intended to identify and quantitate cells expressing cytoplasmic forms of the protein rhBACE-1 |
human |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
BACE-1
(137612)
IgG1
|
R&D Systems
Cat #
MAB-931
Reference:
Wang, 2008 |
immunogen = purified, NS0-derived recombinant human BACE-1 ectodomain (aa 22-460) |
monoclonal mouse
(IgG fract, protein-G, PBS, trehalose, lyophilized) |
ELISA, FC, IC, WB |
rhBACE-1 |
human |
BACE-1
(137612)
IgG1
|
R&D Systems
Cat #
IC-931A |
|
monoclonal mouse
(liquid, PBS, azide, APC conj.) |
FC |
intended to identify and quantitate cells expressing cytoplasmic forms of the protein rhBACE-1 |
human |
BACE-1
(137626)
IgG1
|
R&D Systems
Cat #
MAB-9311 |
immunogen = purified, NS0-derived recombinant human BACE-1 ectodomain (aa 22-460) |
monoclonal mouse
(IgG fract, protein-G, PBS, trehalose, lyophilized) |
ELISA, IP, Neut, WB |
rhBACE-1, ectodomain |
human |
BACE-1
(poly)
|
R&D Systems
Cat #
AF-931 |
immunogen = purified, NS0-derived recombinant human BACE-1 ectodomain |
polyclonal goat
(affinity purified, PBS, lyophilized) |
ELISA, WB |
rhBACE-1 |
human |
BACE-1
(poly)
IgG
|
R&D Systems
Cat #
BAF-931 |
immunogen = purified, NS0-derived recombinant human BACE-1 ectodomain |
polyclonal goat
(affinity purified, Biotin, PBS, lyophilized) |
WB |
BACE-1 |
human |
BACE1 (42)
(poly)
|
I B L Company, Ltd.
Cat# 28051 |
immunogen = synthetic peptide of a part of human BACE1, (R L P R E T D E E P E E P G R R) |
polyclonal rabbit
(purified, PBS, BSA, azide, lyophilized) |
IH |
BACE 1 |
human, rat, mouse |
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