|
Antibodies to Acetylcholine-related Proteins
|
| From the Manufacturers
|
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
Acetyl Choline
(mono)
IgG2aκ
|
Abcam
Cat# ab6470
Discontinued |
immunogen = Choline- glutaric anhydride (GA)-Carriers |
monoclonal mouse
(ascitic fluid, purified, merthiolate) |
IC, IH |
Choline-GA |
rat |
Acetyl choline
(HYChol001)
IgG2aκ
|
Abcam
Cat# ab37010 |
immunogen = Chemical / Small Molecule, Choline-Glutaric acid conjugated to protein carrier |
monoclonal mouse
(ascitic fluid, purified, PBS, Thimerosal) |
ELISA, IC, IH(F), WB |
The antibody specifically recognizes choline and acetylcholine bound by glutaric anhydride to a protein carrier |
|
Acetyl Choline
(poly)
IgG
|
Abcam
Cat# ab34935 |
immunogen = Acetylcholine conjugated to KLH
|
polyclonal rabbit
(liquid, Ammonium Sulphate fractionation, IgG fraction, PBS, azide) |
ELISA, IH(F) |
Acetylcholine |
rat
(expect wide number of species) |
Acetyl- choline
(poly)
IgG
|
ATS
Cat#AB-T02 |
immunogen = synthetic choline glutaric acid conj. to bovine serum albumin |
polyclonal rabbit
(purified, lyophilized, glycerol) |
ELISA, IC, IH(P) |
Choline-GA-Protein, acetylcholine |
non-specific
(all species) |
Acetyl Cholinesterase
(poly)
|
Biodesign Meridian LifeScience
Cat# Q59321G
|
immunogen = acetyl cholinesterase from bovine erythrocytes |
polyclonal goat
(lyophilized, azide, purified) |
ELISA |
acetyl cholinesterase purified or partially purified from erythrocytes |
bovine
(others unknown) |
Acetylcholine
(mono)
IgG2a
|
Chemicon and Upstate are Millipore
Cat# MAB5302 |
immunogen = choline-glutaric anhydride-BSA |
monoclonal mouse
(liquid, azide, purified) |
ELISA, IH(V), WB |
choline-GA-BSA, -AA-BSA, -SA-BSA; 1/>50,000 reactivity with farnesol-BSA, retinoic acid-BSA, myristic acid-BSA, acetycholine and choline |
rat
(others not tested) |
Acetylcholine
(poly)
|
Chemicon and Upstate are Millipore
Cat# AB5522 |
immunogen = acetycholine-glutaric anhydride-GA-PL |
polyclonal rabbit
(liquid, serum, azide) |
IH |
choline-GA-PL, choline-ally alcohol-G-BSA; 1/>10,000 reactivity with phosphatidylcholine, choline and acetylcholine |
rat
(others not tested) |
Acetylcholine
(poly)
|
ISL (Immune Systems Limited)
Cat# 126-4796
Discontinued |
immunogen = KLH conj. acetylcholine |
polyclonal rabbit
(purified Ig) |
IH(P) |
Acetylcholine |
various species |
acetylcholine
(2MG1)
IgG2a, κ
|
US Biological
Cat# A0526-30 |
|
monoclonal mouse
(liquid, glycerol, merthiolate, ammonium sulfate ppt., gel filtration) |
ELISA, IC, IH |
acetylcholine |
rat |
acetylcholine
(poly)
IgG
|
US Biological
Cat# A0526-31 |
immunogen = choline-glutaric anhydride (GA)-BSA |
polyclonal rabbit
(liquid, PBS, glycerol, no preservative, affinity purified) |
ELISA, IC, IH |
acetylcholine |
|
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
ACh
(poly)
|
Anawa
Cat# 0030-2004 (produced by BIOTREND) |
immunogen = acetylcholine conj. with polylysine by glutaraldehyde |
polyclonal rabbit
(liquid, PBS, merthiolate) |
ELISA, IH(F, V) |
Reacts with glutaraldehyde conj. choline (100%) and acetylcholine (40%) |
|
ACh
(poly)
|
Biotrend
Cat# BT00-3020-04 |
immunogen = synthetic acetylchone conjugated to KLH |
polyclonal rabbit
(Ig fract., liquid, PBS, azide) |
ELISA, IH |
specific for acetylcholine |
various species |
ACh
(poly)
|
AbD Serotec
Cat# 0030-2004 |
immunogen = synthetic acetylchone conjugated to KLH |
polyclonal rabbit
(liquid, PBS, azide, affinity purified) |
ELISA, IH(F) |
acetylcholine |
various species |
ACh transporter VAT
(poly)
|
ISL (Immune Systems Limited)
Cat# 126-4798 |
immunogen = seq. -F D G C E D D Y N Y Y S R S conj. to KLH
|
polyclonal sheep
|
|
|
rat |
ACh transporter VAT
(poly)
|
ISL (Immune Systems Limited)
Cat# 126-0008 |
immunogen = Tg conjugate |
polyclonal goat
(purified Ig) |
|
|
rat |
ACh/AChE
Assay Kit
(kit)
|
Molecular Probes Invitrogen
cat# A12217 |
sensitive and stable fluorogenic probe for H2O2 |
(Amplex Red, HRP) |
IF |
AChE levels as low as 0.002 U/mL; ACh levels as low as 0.3 µM |
|
AChE
(190-01)
IgG1,κ
|
Abcam
Cat# ab17774 |
immunogen =
synthetic peptide T D T L D E A E R Q, corresp. to aa 574-583, at C-terminal of human and bovine brain AChE (absent from the erythrocyte enzyme), coupled to PPD with glutaraldehyde
|
monoclonal mouse
(liquid, PBS, azide, protein A/G purified) |
ELISA, WB |
specific for brain AChE; not AChE from erythrocytes |
human, cow
(others not tested) |
AChE
(HR2)
IgG2b
|
Abcam
Cat# ab2803 |
immunogen = purified human cerebellar acetylcholine esterase |
monoclonal mouse
(liquid, ascites, PBS, azide) |
ELISA, IH(F), IP; not WB |
AChE; stains nerve fibers and terminals. Does not stain BChE |
human, guinea pig, rabbit, cat, cow
(others not tested) |
AChE
(HYB111-05)
IgG1,κ
|
Abcam
Cat# ab23455 |
immunogen =
full length protein of brain AChE, purified and detergent solubilized (human)
|
monoclonal mouse
(liquid, PBS, azide, protein G purified) |
ELISA, WB |
acetylcholinesterase; no cross with BtChE |
human
(not Torpedo marmorata or flounder) |
AChE
(ZR3)
IgG2b
|
Abcam
Cat# ab2802 |
immunogen = purified rat brain Acetylcholinesterase |
monoclonal mouse
(liquid, ascites, PBS, azide) |
IH(F), IP; not WB |
soluble and membrane associated AChE; stains nerve fibers and terminals |
rat, guinea pig, rabbit, cat
(others not tested) |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
AChE
(poly)
|
Abcam
Cat# ab7396 |
immunogen = Acetyl Cholinesterase [Bovine Erythrocytes] |
polyclonal goat
(liquid IgG, purified, K phosphate saline, azide) |
DB, ELISA, IP, Neut, WB |
Acetylcholinesterase |
cow
(other species not tested) |
AChE
(poly)
IgG
|
Abcam
Cat# ab7397 |
immunogen = Acetyl Cholinesterase [Bovine Erythrocytes] |
polyclonal goat
(liquid IgG fraction, purified, phosphate, BSA, gentamicin sulfate, HRP conj.) |
DB, ELISA, EM, IH(F), IM, WB |
Acetylcholinesterase |
cow
(other species not tested) |
AChE
(poly)
IgG
|
Abcam
Cat# ab31276 |
immunogen =
synthetic peptide: Q F D H Y S K Q D R C S D L, corresp. to C- terminal aa 601-614 of human Acetylcholinesterase
|
polyclonal goat
(liquid, TBS, BSA, azide, affinity purified) |
ELISA, WB |
Acetylcholinesterase |
human
(expect Mouse, Rabbit, Rat, Cat) |
AChE
(poly)
IgG
|
Abcam
Cat# ab34533 |
Immunogen =
acetylcholinesterase from bovine erythrocytes
|
polyclonal goat
(liquid, PBS, BSA, azide; biotinylated) |
DB, ELISA, IM, WB |
Acetylcholinesterase |
cow
(others not tested) |
AChE
(poly)
IgG
|
Abcam
Cat# ab34936 |
immunogen =
full length, native, acetyl-Cholinesterase from electric eel (purified)
|
polyclonal rabbit
(liquid, PBS, BSA, azide) |
ELISA, WB |
AChE |
electrophorus
(perhaps human at high concentration) |
AChE
(poly)
IgG
|
Abcam
Cat# ab35384 |
immunogen =
native acetylcholine esterase from human RBCs
|
polyclonal sheep
(liquid, PBS, azide) |
ELISA |
Acetylcholine Esterase |
human
(perhaps other species) |
AChE
(poly)
IgG
|
Abcam
Cat# ab35381 |
immunogen =
native from human rbc's
|
polyclonal sheep
(liquid, PBS, MgCl, ZnCl, glycerol, azide; conj. to AP) |
ELISA |
AChE |
human
(perhaps other species) |
AChE
(12B4)
IgG1
|
A B R - Thermo Scientific
Cat#
HYB 111-05-02 |
immunogen = brain acetylcholinesterase, human, purified and detergent solubilized. |
monoclonal mouse
(liquid, PBS, azide, protein A/G purified) |
ELISA, WB |
AChE; no cross-reactivity to BtChE or AChE from flounder or Torpedo marmorata.
|
human |
AChE
(1B4)
IgG3
|
A B R - Thermo Scientific
Cat#
HYB 132-06-02
discontinued |
immunogen = brain acetylcholinesterase from Cercopithecus monkey, purified and detergent solubilized |
monoclonal mouse
(liquid, PBS, azide, protein A/G purified) |
ELISA, WB |
AChE from brain |
human |
AChE
(2E7)
IgG1
|
A B R - Thermo Scientific
Cat#
HYB 101-03-02 |
immunogen = bovine G4-acetylcholinesterase, purified and detergent solubilized |
monoclonal mouse
(liquid, PBS, azide, protein A/G purified) |
ELISA, WB |
AChE; native, heat denatured and SDS plus heat denatured; brain or erythrocytes. No cross-reactivity to BtChE from human serum, AChE from flounder body muscle or Torpedo marmorata.
|
human, bovine |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
AChE
(4E11)
IgG1
|
A B R - Thermo Scientific
Cat#
HYB 190-01-02 |
immunogen = C-terminal 10 residues of human and bovine brain AChE, absent from the erythrocyte enzyme, coupled to PPD with glutaraldehyde. |
monoclonal mouse
(liquid, PBS, azide, protein A/G purified) |
ELISA, WB |
AChE brain, not erythrocytes. Weak cross-reactivity with Torpedo
marmorata AChE but not with AChE from electric eel or human BtChE
|
various species |
AChE
(HR2)
IgG2b
|
A B R - Thermo Scientific
Cat# MA3-042 |
immunogen = purified human cerebellar acetylcholinesterase |
monoclonal mouse
(ascites, PBS, azide) |
ELISA, IH(F), IP; not WB |
AChE in nerve fibers and terminals. This antibody does not detect BChE. |
human, guinea pig, rabbit, cat, bovine |
AChE
(ZR3)
IgG2b
|
A B R - Thermo Scientific
Cat# MA3-041 |
immunogen = purified rat brain acetylcholinesterase |
monoclonal mouse
(ascites, PBS, azide) |
IH, IP; not WB |
AChE in nerve fibers and terminals. Reacts with both the soluble and membrane associated AChE. |
rat, guinea pig, rabbit, cat |
AChE
(HR2)
IgG2b
|
Alexis Enzo Life Sciences
Cat# ALX-802-003 |
immunogen = purified human cerebellar acetylcholinesterase (AChE) |
monoclonal mouse
(liquid, ascites, PBS, azide) |
ELISA, IH(F), IP; not WB |
AChE; no cross-reactivity with butyrylcholine esterase (BChE) |
human, guinea pig, rabbit, cat, bovine |
AChE
(ZR3)
IgG2b
|
Alexis Enzo Life Sciences
Cat# ALX-802-004 |
immunogen = purified rat brain acetylcholine esterase (AChE) |
monoclonal mouse
(liquid, ascites, PBS, BSA, azide, affinity purified) |
IH, IP; not WB |
detects AChE; no cross-reactivity with butyrylcholine esterase (BChE) |
rat, guinea pig, rabbit, cat |
AChE
(HR2)
IgG2b
|
Axxora
Cat# ALX-802-003
produced by Alexis |
immunogen = purified human cerebellar acetylcholinesterase (AChE) |
monoclonal mouse
(liquid, ascites, PBS, azide) |
ELISA, IH(F), IP; not WB |
AChE; no cross-reactivity with butyrylcholine esterase (BChE) |
human, guinea pig, rabbit, cat, bovine |
AChE
(ZR3)
IgG2b
|
Axxora
Cat# ALX-802-004
produced by Alexis |
immunogen = purified rat brain acetylcholine esterase (AChE) |
monoclonal mouse
(liquid, ascites, PBS, BSA, azide, affinity purified) |
IH, IP; not WB |
detects AChE; no cross-reactivity with butyrylcholine esterase (BChE) |
rat, guinea pig, rabbit, cat |
AChE
(AE-1)
IgG1
|
Biotrend
Cat# 0030-2229 |
immunogen = acetylcholine esterase (AChE), purified from human erythrocytes |
monoclonal mouse
(Ig fract., liquid, PBS) |
ELISA, IH |
AChE, neuromuscular junctions. Reactive with different epitope to clone AE-2. |
monkey, human |
AChE
(AE-2)
IgG1
|
Biotrend
cat# 0030-2249 |
immunogen = acetylcholine esterase (AChE), purified from human erythrocytes |
monoclonal mouse
(Ig fract.) |
ELISA, IH, IS, WB |
AChE, neuromuscular junctions. 65KDa band by WB. Reacts with different epitope to clone AE-1. |
monkey, human |
AChE
(HYB 101-03)
IgG1 κ
|
Biotrend
Cat#
HYB 101-03-1 |
immunogen = acetylcholinesterase (AChE) from bovine brain or erythrocytes |
monoclonal mouse
(liquid, Ig fract) |
ELISA, WB |
AChE |
human |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
AChE
(HYB 101-03)
IgG1 κ
|
Biotrend
Cat#
HYB 101-03-02 |
immunogen = acetylcholinesterase (AChE) from bovine brain or erythrocytes |
monoclonal mouse
(liquid, Ig fract) |
ELISA, WB |
AChE |
human |
AChE
(HYB 111-05)
IgG1 κ
|
Biotrend
Cat#
HYB 111-05-1 |
immunogen = acetylcholinesterase (AChE) from human brain |
monoclonal mouse
(liquid, Ig fract) |
ELISA, WB |
AChE |
human |
AChE
(HYB 111-05)
IgG1 κ
|
Biotrend
Cat#
HYB 111-05-02 |
immunogen = acetylcholinesterase (AChE) from human brain |
monoclonal mouse
(liquid, Ig fract) |
ELISA, WB |
AChE |
human |
AChE
(HYB 132-06)
IgG3 κ
|
Biotrend
Cat#
HYB 132-06-1 |
immunogen = acetylcholinesterase (AChE) from human brain |
monoclonal mouse
(liquid, Ig fract) |
ELISA, WB |
AChE |
human |
AChE
(HYB 132-06)
IgG3 κ
|
Biotrend
Cat#
HYB 132-06-02 |
immunogen = acetylcholinesterase (AChE) from human brain |
monoclonal mouse
(liquid, Ig fract) |
ELISA, WB |
AChE |
human |
AChE
(HYB 190-01)
IgG1 κ
|
Biotrend
Cat#
HYB 190-01-1 |
immunogen = acetylcholinesterase (AChE) aa 574-583 from human brain |
monoclonal mouse
(liquid, Ig fract) |
ELISA, WB |
AChE |
bovine |
AChE
(HYB 190-01)
IgG1 κ
|
Biotrend
Cat#
HYB 190-01-02 |
immunogen = acetylcholinesterase (AChE) aa 574-583 from human brain |
monoclonal mouse
(liquid, Ig fract) |
ELISA, WB |
AChE |
bovine |
AChE
(poly)
|
Biotrend
cat# BT00-3022-69 |
immunogen = highly pure acetylcholine esterase from human erythrocytes |
polyclonal sheep
(liquid, Ig fract., PBS, azide) |
ELISA |
recognizes AChE; may work with IH and WB |
human |
AChE
(poly)
IgG
|
Biotrend
Cat# BT00-3022-89 |
immunogen = highly pure acetylcholine esterase from human erythrocytes |
polyclonal sheep
(liquid, Ig fract, PBS, glycerol, azide) |
ELISA |
recognizes AChE; may work with IH and WB |
human
(may react with other species) |
AChE
(poly)
|
Biotrend
Cat#
200-101-232 |
immunogen = acetylcholine esterase from bovine erythrocytes |
polyclonal goat
(lyophilized, IgG fract, potassium phosphate saline, azide, purified) |
DB, ELISA, IA, IP, WB |
AChE |
|
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
AChE
(poly)
|
Biotrend
Cat#
200-106-232 |
immunogen = acetylcholine esterase from bovine erythrocytes |
polyclonal goat
(lyophilized, IgG fract, potassium phosphate saline, BSA, azide, purified, biotinylated) |
ELISA, IB, IH, IM |
AChE |
|
AChE
(poly)
|
Biotrend
Cat#
200-103-232 |
immunogen = human acetylcholine esterase from |
polyclonal goat
(Ig fract, HRP conj.) |
ELISA |
AChE |
|
AChE
(poly)
|
Biotrend
Cat#
100-101-232 |
immunogen = human acetylcholine esterase from |
polyclonal goat
(serum) |
ELISA |
AChE |
|
AChE
(ZR3)
IgG2b
|
Calbiochem EMD Biosciences
Cat# NB04 |
immunogen = purified rat brain acetylcholinesterase |
monoclonal mouse
(liquid, ascites, azide) |
IH(F), IP; not WB |
Recognizes both soluble and membrane-associated acetylcholinesterase. Does not cross-react with butyrylcholinesterase (BChE) |
rat, guinea pig, rabbit, cat
(not mouse, chicken or human) |
AChE
(AE-1)
IgG1
|
Chemicon and Upstate are Millipore
Cat# MAB303 |
immunogen = acetylcholinesterase AChE from human erythrocyte |
monoclonal mouse
(liquid, PBS, azide, purified) |
ELISA, IH, IP |
acetylcholinesterase |
monkey, human |
AChE
(AE-2)
IgG1
|
Chemicon and Upstate are Millipore
Cat# MAB304 |
immunogen = acetylcholinesterase AChE from human erythrocyte |
monoclonal mouse
(liquid, ascites, PBS, azide) |
IH, IP |
acetylcholinesterase |
monkey, human, rat, chicken |
AChE
(AR-1)
IgG1
|
Chemicon and Upstate are Millipore
Cat# MAB337 |
immunogen = acetylcholinesterase AChE from human erythrocyte |
monoclonal mouse
(liquid, ascites, PBS, glycerol, HEPES, Tris-HCl, BSA, PVP-40, Tween 20, azide) |
ELISA, IH, RIA; not WB |
acetylcholinesterase |
monkey, human, rat |
AChE
(AE-2)
IgG
|
DSHB U. Iowa
Cat# AE-2 |
immunogen = adult human erythrocyte acetyl cholinesterase |
monoclonal mouse
|
IH, IP, IS |
Cholinesterase in muscle, neuron, erythrocyte |
primate (various), guinea pig, dog, calf
(not rat, chicken, frog) |
AChE
(AE-3)
IgG
|
DSHB U. Iowa
Cat# AE-3 |
immunogen = adult human erythrocyte acetyl cholinesterase |
monoclonal mouse
|
IH, IP |
Cholinesterase in muscle, neuron, erythrocyte |
primate (various), guinea pig, dog
(not rat, calf, chicken, frog) |
AChE
(C1B7)
IgG, κ
|
DSHB U. Iowa
Cat# C1B7 |
immunogen = human erythrocyte membrane acetyl cholinesterase |
monoclonal mouse
|
IP, IS |
binds to erythrocyte ghosts |
human |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
AChE
(ZR3)
IgG2b
|
EMD Biosciences, Inc.
cat# NB04 |
immunogen = purified rat brain acetyl cholinesterase |
monoclonal mouse
(liquid, diluted ascites, azide) |
IH(F), IP; not WB |
reacts with both the soluble and membrane associated enzyme, and does not crossreact with BChE |
rat, guinea pig, rabbit, cat
(not human, mouse, chicken) |
AChE
(poly)
|
Everest Biotech Ltd
Cat# EB06659 |
immunogen = peptide with seq. Q F D H Y S K Q D R C S D L, from the C-terminus of the protein |
polyclonal goat
(liquid IgG, purified, Tris saline, BSA, azide) |
ELISA, WB |
Acetylcholinesterase |
human
(expect mouse, rat, cat, rabbit from sequence similarity) |
AChE
(AE-1)
IgG1
|
ISL (Immune Systems Limited)
Cat# 126-0001 |
immunogen = native AChE from erythrocytes |
monoclonal mouse
(Ig-IE) |
ELISA |
AChE |
human |
AChE
(AE-2)
IgG1
|
ISL (Immune Systems Limited)
Cat# 126-0002 |
immunogen = native AChE |
monoclonal mouse
(purified Ig) |
ELISA |
AChE |
|
AChE
(poly)
|
ISL (Immune Systems Limited)
Cat# 126-0003 |
immunogen = native from RBCs |
polyclonal sheep
(Ig-AS) |
ELISA |
AChE |
human |
AChE
(poly)
|
ISL (Immune Systems Limited)
Cat# 126-0004 |
immunogen = native AChE from RBCs |
polyclonal sheep
(Ig-AP) |
ELISA |
AChE |
human |
AChE
(poly)
IgG
|
LifeSpan Biosciences, Inc.
Cat# LS-C18743 |
immunogen = acetyl cholinesterase [bovine erythrocytes] |
polyclonal goat
(lyophilized, KPBS, azide) |
ELISA, IF, IP, WB |
Acetylcholinesterase |
bovine |
AChE
(ZR3)
IgG2b
|
Novagen EMD Chemicals, Inc.
Cat# NB04
(produced by Calbiochem) |
immunogen = purified rat brain acetylcholinesterase |
monoclonal mouse
(liquid, diluted ascites, azide) |
IH(F), IP; not WB |
recognizes both soluble and membrane-associated acetylcholinesterase. No cross-reaction with BChE (butyrylcholinesterase) |
rat, guinea pig, rabbit, cat
(not mouse, chicken or human) |
AChE
(HR2)
IgG2b
|
Novocastra
Cat# NCL-AChE |
immunogen = purified human cerebellar acetylcholinesterase |
monoclonal mouse
(lyophilized, supernatant, azide) |
IH(F); not IH(P) |
AChE |
human |
AChE
(mono)
IgG1, κ
|
Novus Biologicals
cat# NBP1-05073 |
immunogen = bovine G4-acetyl-cholinesterase, purified and detergent solubilized |
monoclonal mouse
(liquid, ascites, PBS, azide, protein A purified) |
ELISA, WB |
specificity for native, heat denatured and SDS plus heat denatured AChE |
bovine |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
AChE
(mono)
IgG1, κ
|
Novus Biologicals
cat# NBP1-05139 |
immunogen = human brain acetyl-cholinesterase, purified and detergent solubilized |
monoclonal mouse
(liquid, ascites, PBS, azide, protein A purified) |
ELISA, WB |
specificity for acetylcholinesterase; no cross-reaction with BtChE |
human
(not flounder or torpedo marmorata) |
AChE
(mono)
IgG1, κ
|
Novus Biologicals
cat# NBP1-05146 |
immunogen = C-terminal 10 residues (aa 574-583) of brain acetylcholinesterase (human and bovine), absent from the erythrocyte enzyme |
monoclonal mouse
(liquid, ascites, PBS, azide, protein A purified) |
ELISA, WB |
specific for brain not erythrocyte AChE; no cross-reaction with BtChE |
human, bovine
(weak reactivity to torpedo marmorata; not electric eel) |
AChE
(mono)
|
Novus Biologicals
cat# H00000043-B01
supplied by Abnova |
immunogen = ACHE (AAH94752, 1-640 aa) full-length human protein |
monoclonal mouse
(liquid, antiserum, glycerol, no preservative) |
ELISA, WB |
AChE - acetylcholinesterase; reactive against recombinant protein with GST tag |
human |
AChE
(2C3)
IgG2a, κ
|
Novus Biologicals
cat# H00000043-M02
supplied by Abnova |
immunogen = AChE (NP_000656, aa515- 615) partial recombinant protein with GST tag |
monoclonal mouse
(liquid, ascites, purified, no preservative) |
ELISA, WB |
AChE - acetylcholinesterase (YT blood group) |
human |
AChE
(AE-2)
IgG1
|
Novus Biologicals
cat# NB100-65260 |
immunogen = Acetylcholinesterase (AChE) from human erythrocytes |
monoclonal mouse
(liquid, PBS, ascites, azide) |
IH(F), IP |
Acetylcholinesterase |
human |
AChE
(poly)
IgG
|
Novus Biologicals
cat# NB 100-1519 |
immunogen = synthetic peptide Q F D H Y S K Q D R C S D L, representing the C-terminus of the human protein according to NP_000656. |
polyclonal goat
(liquid, purified) |
ELISA, WB |
Acetylcholine esterase |
human
(expect rat, mouse, cat, rabbit from homology) |
AChE
(poly)
IgG
|
Novus Biologicals
cat# NB 600-911 |
immunogen = acetyl cholinesterase [bovine erythrocytes] |
polyclonal goat
(liquid, PBS, azide, affinity purified) |
ELISA, IP, WB |
Acetylcholine Esterase |
bovine |
AChE
(poly)
IgG
|
Novus Biologicals
cat# NB 100-2014 |
immunogen = acetyl cholinesterase [bovine erythrocytes] |
polyclonal goat
(liquid, PBS, azide, BSA, thimerisol; conj. to HRP) |
ELISA, IF, WB |
Acetylcholine Esterase |
bovine |
AChE
(poly)
IgG
|
Novus Biologicals
cat# NB100-62292 |
immunogen = native from erythrocytes |
polyclonal sheep
(liquid, PBS, azide) |
ELISA |
Acetylcholinesterase |
human |
AChE
(poly)
IgG
|
Novus Biologicals
cat# NB100-62293 |
immunogen = native from erythrocytes |
polyclonal sheep
(liquid, PBS, azide, conj. to AP) |
ELISA |
Acetylcholinesterase |
human |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
AChE
(poly)
IgG
|
Novus Biologicals
cat# NB100-66375 |
immunogen = highly purified acetylcholine esterase from Electrophorus electricus. EC no. 3.1.1.7 |
polyclonal rabbit
(liquid, ascites, azide, protein G purified) |
ELISA, WB |
Acetylcholinesterase |
human, electrophorus |
AChE
(poly)
|
Novus Biologicals
cat# H00000043-D01P
supplied by Abnova
MaxPab antibody |
immunogen = ACHE (AAH94752.1, aa1-640) full-length human protein |
polyclonal rabbit
(liquid, protein A purified, no preservative) |
ELISA, WB |
reactive against AChE - acetylcholinesterase recombinant protein with GST tag |
human |
AChE
(46)
IgG1
|
BD Pharmingen
Cat# 610267 |
immunogen = aa. 411-609 at the C-terminal of human AChE |
monoclonal mouse
(glycerol, PBS, BSA, azide, positive control) |
IF, IH, IP, WB |
68 kDa acetyl cholinesterase |
human, rat, mouse, dog, chicken |
AChE
(HR2)
IgG2b
|
RDI /Fitzgerald
cat# 10R-A104A
formerly RDI-ACHEXabm |
immunogen = purified human cerebellar AChE protein |
monoclonal mouse
(purified, ascites, PBS, BSA, azide) |
IF, IP, WB |
acetyl cholinesterase |
human, rat, guinea pig, mouse, rabbit, cat, bovine |
AChE
(mono)
IgG1
|
Santa Cruz
Cat# sc-32283 |
immunogen = human erythrocyte acetylcholinesterase (AchE) |
monoclonal mouse
(PBS, gelatin, azide) |
IF |
AChE |
human |
AChE
(poly)
|
Santa Cruz
Cat# sc-6430 |
immunogen = peptide mapping at the C-terminus of acetylcholine esterase (AchE) of human origin |
polyclonal goat
(IgG in PBS, affinity purified, gelatin, azide; blocking peptide [sc-6430P]) |
IF, IH, WB |
AChE |
human, rat, mouse |
AChE
(poly)
|
Santa Cruz
Cat# sc-6431 |
immunogen = peptide mapping at the N-terminus of acetylcholine esterase (AchE) of human origin |
polyclonal goat
(IgG in PBS, affinity purified, gelatin, azide; blocking peptide [sc-6431]) |
IF, IP, WB |
AChE |
human |
AChE
(poly)
|
Santa Cruz
Cat# sc-6432 |
immunogen = peptide mapping at the N-terminus of acetylcholine esterase (AchE) of mouse origin |
polyclonal goat
(IgG in PBS, affinity purified, gelatin, azide; blocking peptide [sc-6432P]) |
IF, IP, WB |
AChE |
rat, mouse |
AChE
(poly)
|
Santa Cruz
Cat# sc-11409 |
immunogen = recombinant protein corresp. to aa 481-614 mapping at the C-terminus of acetylcholine esterase (AchE) of human origin. |
polyclonal rabbit
(IgG in PBS, gelatin, azide) |
IF, IP, WB |
AChE |
human, rat, mouse |
AChE
(2C3)
IgG2a
|
AbD Serotec
Cat# MCA2900Z |
immunogen = recombinant protein corresp. to aa 515 - 615 of human AChE |
monoclonal mouse
(liquid, PBS, azide free, protein A purified) |
WB |
AChE |
human, mouse |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
AChE
(AE-2)
IgG1
|
AbD Serotec
Cat# OBT1641 |
immunogen = acetylcholinesterase (AChE) from human erythrocyte |
monoclonal mouse
(liquid, ascites, PBS, azide) |
IH(F), IP |
AChE |
human |
AChE
(poly)
|
AbD Serotec
Cat# 0200-0042 |
immunogen = native, acetylcholinesterase |
polyclonal rabbit
(liquid, PBS, BSA, azide, [NH4]2[SO4]) |
ELISA, WB |
AChE (72kDa); may react with ACh (68kDa) in RBC |
eel
(expect human) |
AChE
(poly)
|
AbD Serotec
Cat# 0030-2269 |
immunogen = native, acetylcholinesterase from erythrocytes |
polyclonal sheep
(liquid, PBS, azide, [NH4]2[SO4]) |
ELISA |
AChE |
human |
AChE
(poly)
|
AbD Serotec
Cat# 0030-2289 |
immunogen = native, acetylcholinesterase from erythrocytes |
polyclonal sheep
(liquid, PBS, glycerol, ZnCl2, MgCl2, azide, [NH4]2[SO4]; AlkPhos) |
ELISA |
AChE |
human |
AChE
(2C3)
IgG2a, κ
|
Sigma Aldrich
Cat# WH0000043M2 |
immunogen = AChE aa515-615 partial recombinant protein with GST tag, NP_000656 |
monoclonal mouse
(liquid, PBS, purified) |
IB, IH, IP |
AChE |
human, mouse |
AChE
(ZR3)
IgG2b
|
Sigma Aldrich
Cat# A6970 |
immunogen = rat brain acetylcholinesterase |
monoclonal mouse
(liquid, PBS, BSA, azide, purified) |
IH, IP |
AChE |
rat, guinea pig, rabbit, cat
(not chicken, mouse or human) |
AChE
(poly)
IgG1
|
Sigma Aldrich
Cat# HPA019704 |
immunogen = acetylcholinesterase precursor recombinant protein epitope signature tag (PrEST) |
polyclonal rabbit
(liquid, PBS, glycerol, azide, affinity purified) |
IH(P) |
AChE |
human |
AChE
(2Q6)
IgG1
|
US Biological
Cat# A0526-42B |
immunogen = acetylcholinesterase (AChE) from lysed human erythrocyte |
monoclonal mouse
(liquid, PBS, azide, affinity purified) |
ELISA |
acetylcholinesterase |
monkey, human |
AChE
(2Q7)
IgG1
|
US Biological
Cat# A0526-42A |
immunogen = acetylcholinesterase (AChE) from lysed human erythrocyte |
monoclonal mouse
(liquid, ascites, PBS, azide) |
ELISA |
acetylcholinesterase |
monkey, human |
AChE
(4i348)
IgG2b
|
US Biological
Cat# A0526-42D |
immunogen = purified human cerebellar acetylcholinesterase |
monoclonal mouse
(liquid, ascites, PBS, azide) |
ELISA, IH, IP; not WB |
acetylcholinesterase; not butyrylcholinesterase (BChE) |
human, guinea pig, rabbit, cat, bovine |
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