| Antibodies to Neuron Specific Enolase Proteins | ||||||
| From The Manufacturers | ||||||
| Antigen (Clone) IgG |
Distributor (Producer) |
Epitope (Immunogen) |
Host (Formulation) |
Methods | Specificity | Reactivity |
| NSE
(-poly) IgG |
Abcam Cat# ab834 |
immunogen = NSE (gamma-gamma isoenzyme) isolated from bovine brain | Polyclonal rabbit (liquid, whole antiserum, azide) |
IH(F,P) | human isoenzymes of NSE containing gamma subunits | Human, cow; others not tested |
| NSE
(-poly) IgG |
Abcam Cat# ab944 |
immunogen = NSE isolated from bovine brain | Polyclonal rabbit (liquid, protein-A, PBS, azide, prediluted) |
IH(F,P) | gamma-enolase isoenzymes | Human, cow; others not tested |
| NSE
(E27) IgG1 |
Abcam Cat# ab6260 |
immunogen = purified neuron specific enolase from human brain | Monoclonal Mouse (liquid, protein-A, PBS, azide) |
IH(F,P) | gamma-gamma enolase isozyme of 46 kD | Human; others not tested |
| NSE
(MIG-N3) IgG1κ |
Abcam Cat# ab8197 |
immunogen = purified human gamma-gamma enolase | Monoclonal Mouse (liquid, protein-A, PBS, BSA, azide) |
IH(P), WB |
binds to human gamma-gamma enolase and to material from guinea pigs; does not bind to serum protein A | Human; others not tested |
| NSE
(5G10) IgG2b |
Abcam Cat# ab8324 |
immunogen = purified NSE from human brain | Monoclonal Mouse (liquid, protein-G, PBS, azide) |
ELISA, IH(P) |
45 kD band corresponding to NSE | Human; others not tested |
| NSE
(1C1) IgG2a |
Abcam Cat# ab8328 |
immunogen = purified NSE from human brain | Monoclonal Mouse (liquid, protein-G, PBS, azide) |
ELISA | 45 kD band corresponding to NSE | Human; others not tested |
| NSE
(5A4) IgG2a |
Abcam Cat# ab10169 |
immunogen = recomb. full length human protein | Monoclonal Mouse (liquid, protein-G, PBS, azide) |
ELISA, IH(P), WB |
NSE | Human; others not tested |
| NSE
(5E2) IgG2a |
Abcam Cat# ab10171 |
immunogen = full length native human protein, purified | Monoclonal Mouse (liquid, protein-G, PBS, azide) |
ELISA, IH(P), WB |
NSE | Human; others not tested |
| NSE
(BBS/NC/ VI-H14) IgG1 |
Abcam Cat# ab11097 |
immunogen = purified human gamma enolase | Monoclonal Mouse (liquid, protein-A, BSA, azide) |
IH(P) | gamma enolase; does not recognise the alpha or beta isoenzymes of enolase | Human, rat, dog, guinea pig; others not tested |
| NSE (V1-H14) IgG1k |
Accurate | immunogen = gamma-gamma enolase purified from human brain | Monoclonal Mouse (liquid, supernat, Tris, azide) | IB, IH(P) | gamma-gamma-enolase (46kDa), not alpha- or beta- subunits of NSE | human |
| NSE (-mono) IgG1k |
Accurate | immunogen = purified human NSE | Monoclonal (predilute, buffered) | ELISA, IH(F,P) |
NSE in neurons and their axonal and dendritic processes in the brain | |
| NSE (-poly) |
Accurate | immunogen = bovine neuron specific enolase | Polyclonal Rabbit (predilute, buffered) | IH(F,P) | gamma-gamma or alpha-gamma isoenzymes of NSE, in brain, Langerhans islets and peripherial nerves of skin | Human |
| NSE (5E2) IgG2a |
Accurate | immunogen = purified NSE from human brain. | Monoclonal Mouse (lyophilized) | IH(F,P) | gamma gamma enotase enzyme which is present in neurons, ganglion cells and neuroendocrine (APUD) cells | human, guinea pig |
| NSE (5E2) IgG2a |
Accurate | Monoclonal Mouse (purified Ig, PBS, glycrol, azide) | IH(P), WB | binds neuronal and adrenal tumours and other neuroendocrine tumours of epithelial origin | human, guinea pig | |
| NSE (IC1, 5A4, 5E2, 5G10) IgG2a, IgG2b(5G10) |
Advanced Immunochem cat# M-NSE3 |
immunogen = Neuron-Specific Enolase | Monoclonal Mouse (purified, PBS, azide) | EIA, IH(F,P) |
45kDa NSE | Human |
| NSE (-poly) IgG |
Biodesign | immunogen = gamma-gamma isoenzyme isolated from human brain | Polyclonal Rabbit (sera, IgG fract) | IC, IH(F,P) | NSE of human and other mammalian species; no cross-reaction with the alpha subunit, may be cross-reaction with the beta subunit | Human, mammalian |
| Antigen (Clone) IgG |
Distributor (Producer) |
Epitope (Immunogen) |
Host (Formulation) |
Methods | Specificity | Reactivity |
| NSE (21) IgG1 |
Biodesign | immunogen = gamma-enolase purified from human brain | Monoclonal Mouse (purified, protein-A, buffered) | gamma-enolase (enzyme active site) | ||
| NSE (17, 19) IgG1 |
Biodesign | immunogen = gamma-enolase | Monoclonal Mouse (purified, buffered, no preserve) | gamma-enolase | ||
| NSE,g (5G10) IgG2b |
Biodesign | immunogen = purified neuron-specific enolase from human brain | Monoclonal Mouse (purified, protein-A, PBS, azide) | ELISA, IB, IH(P) |
NSE | Human |
| NSE,g (5E2) IgG2a |
Biogenesis | immunogen = bovine neuron specific enolase | Polyclonal Rabbit (predilute, IgG, PBS, azide) | IH(F,P) | gamma-gamma or alpha-gamma isoenzymes of NSE, in most neurons | Human |
| NSE (-protein) |
Biogenesis | source = NSE from human brain | (liquid, purified, Tris, mercapto) | 50-55 kDa NSE (SDS-PAGE) | ||
| NSE,g (5E2) IgG2a |
Biogenesis | immunogen = purified NSE from human brain | Monoclonal Mouse (liquid, purified, Ig-fract, PBS, no preserve) | EIA, IB, IH(P) |
45 kDa NSE | Human |
| NSE (5E2) IgG2a IgG2a |
Biomeda | immunogen = purified human NSE | Monoclonal Mouse (predilute) | IH(F,P) | human 46 kD gamma gamma enolase enzyme which is present in neurons, ganglion cells and neuroendocrine (APUD) cells | Human, Guinea Pig. Others not tested |
| NSE (-poly) Ig |
Biomeda | immunogen = purified human NSE | polyclonal Rabbit (predilute) | ELISA, IH(P) |
gamma gamma enolase enzyme which is present in neurons, ganglion cells and neuroendocrine (APUD) cells | Human, Bovine, Horse, Porcine, sheep, Rabbit, Rat, Guinea Pig, Chicken, Dog. Others not tested. |
| Antigen (Clone) IgG |
Distributor (Producer) |
Epitope (Immunogen) |
Host (Formulation) |
Methods | Specificity | Reactivity |
| NSE (5E2) IgG2a |
Biomeda | immunogen = purified NSE from human brain | Monoclonal Mouse (dehydtated, concentrated) | IH(F,P) | 46 kDa gamma gamma enolase enzyme which is present in neurons, ganglion cells and neuroendocrine (APUD) cells | Human |
| NSE (-poly) Ig |
Biomeda | immunogen = Neuron specific enolase isolated from bovine brain | polyclonal Rabbit (dehydrated) | IH(F,P), WB | neurons and their axonal and dendritic processes in brain tissue and with the Langerhans islets in the pancreas and peripheral nerves in the skin | Bovine |
| NSE (5E2) IgG2a |
Biomeda | immunogen = purified NSE from human brain | Monoclonal Mouse (bovine serum, Tris, BMC-6, azide) | IH(F,P) | 46 kDa gamma gamma enolase enzyme which is present in neurons, ganglion cells and neuroendocrine (APUD) cells | Human |
| NSE (-poly) Ig |
Biomeda | immunogen = NSE isolated from bovine brain | polyclonal Rabbit (Tris, azide, concentrate) | IH(F,P), WB |
isozymes of NSE which contain gamma-subunits, in brain, pancreas | Human, Bovine, Horse, Porcine, sheep, Rabbit, Rat, Guinea Pig, Chicken, Dog. Others not tested. |
| NSE (-poly) Ig |
Chemicon | immunogen = NSE isolated from bovine brain | poplyclonal Rabbit (Tris, azide, concentrate) | IH(F,P), WB |
isozymes of NSE which contain gamma-subunits, in brain, pancreas | Human, Bovine, Horse, Porcine, sheep, Rabbit, Rat, Guinea Pig, Chicken, Dog. Others not tested. |
| NSE-gg (-mono) IgG1 |
Chemicon | immunogen = rat gg-neuron specific enolase | monoclonal Mouse (IgG, purified, Tris acetate) | IH | gg-NSE, No cross-reactivity with aa-NSE, bb-NSE or CK-BB. | Human, Bovine, Rat |
| Antigen (Clone) IgG |
Distributor (Producer) |
Epitope (Immunogen) |
Host (Formulation) |
Methods | Specificity | Reactivity |
| NSE-gg (5E2) IgG2a |
Chemicon | monoclonal Mouse (ascites, protein-A, PBS azide) | IC, IH, WB |
human gamma enolase polypeptide of 45 kDa found on neuronal and adrenal tumours, e.g. phaeochromo cytomas, paragangliomas, neuroblastomas and other neuroendocrine tumours of epithelial origin | Human, guinea pig. Others not tested. | |
| NSE (BBS/NC/ VI-H14) IgG1,k |
DAKO Cat# M0873 |
immunogen = gg-enolase purified from human brain | Monoclonal Mouse (liquid, supernat, Tris, azide) | IC(P) | normal and neoplastic cells of neuronal and neuroendocrine origin; neurons in human cerebral cortex and brainstem nuclei; ganglion cells in the human gastrointestinal tract and myelinated and unmyelinated nerve fibers | Human, guinea pig |
| g-NSE (BBS/NC/ VI-H14) IgG1,k |
DAKO Cat# M1557 |
immunogen = gg-enolase purified from human brain | Monoclonal Mouse (liquid, supernat, Tris, azide) | IH(F,P) | g-subunits of enolase in neurons of the cerebral cortex, brainstem nuclei, in neuronal cytoplasm and processes; not with the a- or b-subunit. Cytoplasmic staining | Human, rat, guinea pig |
| g-NSE
(-poly) |
DAKO Cat# N1516 |
immunogen = 90 kDa homodimer gg-enolase from bovine brain | Polyclonal rabbit (serum, Tris, azide) | IH(F,P) | human isoenzymes of neuron specific enolase which contain gamma-subunits; cytoplasmic staining | Human, bovine? |
| NSE (M09202) IgG1 |
Fitzgerald | immunogen = human NSE | monoclonal Mouse (ascites, purified, Tris acetate) | 45 kDa gamma subunits of NSE of neuronal origin but not with the alpha subunits of glial origin. It may cross react with beta subunits from muscle | Human, rat, bovine. | |
| NSE (M32231) IgG2b |
Fitzgerald | immunogen = human NSE | monoclonal Mouse (ascites, affinity purified, PBS, azide) | ELISA, IB, IC |
gamma subunits of NSE of neuronal origin but not with the alpha subunits of glial origin. It may cross react with beta subunits from muscle | Human |
| NSE (M32233) IgG2a |
Fitzgerald | immunogen = human NSE | monoclonal Mouse (ascites, purified, PBS, azide) | ELISA, IB, IC |
gamma subunits of NSE of neuronal origin but not with the alpha subunits of glial origin. It may cross react with beta subunits from muscle | Human |
| NSE (M32234) IgG2a |
Fitzgerald | immunogen = human NSE | monoclonal Mouse (ascites, purified, PBS, azide) | ELISA, IB, IC |
gamma subunits of NSE of neuronal origin but not with the alpha subunits of glial origin. It may cross react with beta subunits from muscle | Human |
| NSE (-protein) |
Fitzgerald | source = human brain | (NaH2PO4 buffer, pH 7.4 with 0.15M NaCl and 5mM MgSO4) | Raw donor materials were tested negative to HBsAg, HIV 1/2 and HCV | ||
| Antigen (Clone) IgG |
Distributor (Producer) |
Epitope (Immunogen) |
Host (Formulation) |
Methods | Specificity | Reactivity |
| NSE (-poly) |
Harlan | immunogen = highly purified g-g-enolase from bovine brain | Polyclonal Rabbit (sera, PBS, azide) | ELISA, IH, WB |
NSE | guinea-pig, rat, mouse, pig, gerbil, cat, rabbit, dog, sheep, cow, horse |
| NSE (MIG-N3) IgG1k |
Harlan | Monoclonal Mouse (purified Ig, azide) | IH(P), WB |
gg-enolase (Mw46kD); labels neurons, APUD cells, ganglion cells in the GI tract, myelinated and unmyelinated nerve fibres. | human, guinea-pig | |
| NSE (5E2) IgG2a |
Harlan | Monoclonal Mouse (purified, PBS, glycerol, azide) | IH(P), WB |
Neuron Specific Enolase (Mw68kD) | human, guinea-pig | |
| NSE (5E2) IgG2a |
Upstate Biotech | immunogen = purified human NSE (from brain) | Monoclonal Mouse (purified, protein-A) | IC, not WB |
48 kDa Neuron Specific Enolase | human, guinea-pig, monkey |
| NSE (NSE-1G4) IgG2ak |
Zymed | immunogen = Bovine brain | Monoclonal Mouse (purified, predilute) | IH(F,P) | gamma subunits of NSE of neuronal origin but not with the alpha subunits of glial origin; it may cross react with beta subunits from muscle | |
| NSE (NSE-1G4) IgG2ak |
Zymed | immunogen = Bovine brain | Monoclonal Mouse (2nd gen. predilute, titered, for use with Histostain kits) | IH(F,P) | gamma subunits of NSE of neuronal origin but not with the alpha subunits of glial origin; it may cross react with beta subunits from muscle | |
| NSE (NSE-1G4) IgG2ak |
Zymed | immunogen = Bovine brain | Monoclonal Mouse (liquid, titered, ready to use) | IH(F,P) | gamma subunits of NSE of neuronal origin but not with the alpha subunits of glial origin; it may cross react with beta subunits from muscle | |
| NSE (NSE-1G4) IgG2ak |
Zymed | immunogen = Bovine brain | Monoclonal Mouse (liquid, concentrate) | IH(F,P) | gamma subunits of NSE of neuronal origin but not with the alpha subunits of glial origin; it may cross react with beta subunits from muscle | |
| Antigen (Clone) IgG |
Distributor (Producer) |
Epitope (Immunogen) |
Host (Formulation) |
Methods | Specificity | Reactivity |
