| Antibodies to Glial Fibrillary Acidic Proteins | ||||||
| Antigen (Clone) IgG |
Distributor (Producer) |
Epitope (Immunogen) |
Host (Formulation) |
Methods | Specificity | Reactivity |
| GFAP
(-poly) |
Abcam Cat# AB7260 |
immunogen = native GFAP purified from pig spinal cord | Polyclonal rabbit (liquid, whole serum, azide) | IC, IF, IH, WB |
55 kDa GFAP, astrocytic cells | Human, rat, mouse, cat, pig, cow, mammalian |
| GFAP
(-poly) |
Abcam Cat# AB7779 |
immunogen = GFAP from bovine spinal cord | Polyclonal rabbit (liquid, whole serum, Tris, BMC-6, azide) | IH(F,P) | astrocytes in brain; in PNF, Shwann cells, satellite cells, and enteric glial cells | |
| GFAP
(-poly) |
Abcam Cat# AB929 |
immunogen = GFAP isolated from cow spinal cord | Polyclonal rabbit (liquid, PBS, azide, predilute) | IH(F,P) | astrocytes and some ependymal cells in brain; in PNF, Shwann cells, satellite cells, and enteric glial cells | Human, cat, chicken, cow, guinea pig, hamster, monkey, rat, mouse, sheep, kangaroo |
| GFAP
(GF-01) IgG1 |
Abcam Cat# AB7806 |
immunogen = pellet of pig brain cold-stable proteins after depolymer- ization of microtubules | Monoclonal Mouse (liquid, PBS) | ELISA, IC, IH(F,P), WB |
GFAP in astrocytes | Human, pig; not rat, mouse |
| GFAP
(2A5) IgG1k |
Abcam Cat# AB8136 |
immunogen = native GFAP purified from pig spinal cord | Monoclonal Mouse (tissue culture supernat) | ELISA, IF, IH(P), WB |
GFAP in astrocytic cells; 55 kDa in WB. | Human, mammalian |
| GFAP
(6F2) IgG1 |
Abcam Cat# AB8975 |
immunogen = GFAP from human brain | Monoclonal Mouse (liquid, tissue culture supernat, azide) | IH(F,P), WB |
GFAP in astrocytic cells in CNS, Schwann cells in PNS | Human; others not tested |
| GFAP
(GA5) IgG1 |
Abcam Cat# AB9551 |
immunogen = GFAP isolated from porcine spinal cord | Monoclonal Mouse (purified Ig, PBS, preserved) | IH(P) | GFAP in astrocytes, ependymal cells and corresponding tumors; no cross react with other intermediate filaments | Human, pig, rat, chicken; others not tested |
| GFAP
(GF-01) IgG1 |
Accurate Cat# YM3012 |
human spinal cord GFAP | Monoclonal Mouse (supernatant, sodium azide) | IH(F,P) | 50 kDa GFAP, cytoplasm | Human, swine |
| GFAP
(-poly) |
Accurate Cat# BMD209M reference (rabbit GFAP) Sun, 2001 |
bovine spinal cord GFAP | Polyclonal rabbit (serum, purified) | IH(F,P) | GFAP, astrocytes, schwann cells, entire glial cells | Human |
| GFAP
(6F2) |
Accurate cat# ICC-MGF |
monoclonal | 56 kDa GFAP | |||
| GFAP
(6F2) IgG1 |
Accurate Cat# YM3002 |
Monoclonal Mouse (supernatant, sodium azide) | FC,
IH(F,P) |
56 kDa GFAP | Human, mouse | |
| GFAP
(-poly) |
Accurate cat# ICC-PGF |
Polyclonal rabbit | 56 kDa GFAP | |||
| GFAP
(G-A-5) |
Accurate cat# MAS-410p |
monoclonal
(purified) |
IH(F,P) | Bergman Glial Cell, astrocyte | Human,
rat, swine |
|
| GFAP
(6F2) |
Accurate cat# AXL-884M |
monoclonal | Human | |||
| GFAP
(-poly) |
Accurate Cat# YMPS032 |
Polyclonal rabbit (serum) |
IH(F,P) | 56 kDa GFAP | Human,
bovine, rat |
|
| Antigen (Clone) IgG |
Distributor (Producer) |
Epitope (Immunogen) |
Host (Formulation) |
Methods | Specificity | Reactivity |
| GFAP
(6F2) |
Accurate cat# AXL-7161M |
monoclonal | HRP | Human | ||
| GFAP
(G-A-5) |
Accurate cat# BYA-6077-1 |
monoclonal | ||||
| GFAP
(G-A-5) |
Accurate cat# MED-CLA100 |
monoclonal (supernatant) | IH(P) | Human | ||
| GFAP
(GFP/6F2) |
Accurate cat# MED-CLA75/5 |
Monoclonal
(supernatant) |
IH(P) | Human | ||
| GFAP
(-poly) |
Accurate cat# BMD-02-209G |
Polyclonal rabbit | HRP (kit,
25 tests) |
Human | ||
| GFAP
(-poly) |
Accurate cat# BMD-02-209M |
Polyclonal rabbit | HRP (kit) | Human | ||
| GFAP
(-poly) |
Accurate cat# BMD-02-209 |
Polyclonal rabbit | HRP (kit) | Human | ||
| GFAP | Accurate cat# BMD-020D |
monoclonal (prediluted) |
Human, | |||
| GFAP
(-poly) |
Accurate cat# BYA-1077-1 |
Polyclonal rabbit (IgG fract) |
Human | |||
| Antigen (Clone) IgG |
Distributor (Producer) |
Epitope (Immunogen) |
Host (Formulation) |
Methods | Specificity | Reactivity |
| GFAP
(-poly) |
Accurate cat# BMD-N076 |
Polyclonal rabbit (lyophilized, reconstitution buffer) |
IH(F,P) | |||
| GFAP
(-poly) |
Accurate cat# BMD-V2023 |
Polyclonal rabbit (liquid) |
Bovine | |||
| GFAP
(-poly) |
Accurate cat# AXL-1935 |
Polyclonal rabbit (ready-to-use) |
for PAP | Bovine | ||
| GFAP
(-poly) |
Accurate cat# AXL-457 |
Polyclonal rabbit | Hu, Bov, Cat, Rat, Chick, Guinea pig, Ham, Shp, Kang, Mon, Mouse | |||
| GFAP
(-poly) |
Accurate cat# BMD-209M |
Polyclonal rabbit (prediluted) |
Human | |||
| GFAP
(-poly) |
Accurate cat# BMD-A51 |
Polyclonal rabbit (concentrate) |
Human | |||
| GFAP
(G-A-5) |
Accurate cat# BYA-6377-1 |
monoclonal (purified) |
IH(P),
(C-OH fix) |
Hu,Sw,Cat,
Rat,Ch., Goldfish, Octopus,Snail, |
||
| Antigen (Clone) IgG |
Distributor (Producer) |
Epitope (Immunogen) |
Host (Formulation) |
Methods | Specificity | Reactivity |
| GFAP
(GF5) IgG2b |
Advanced Immunochem cat# aichHG2b-GF5 |
Monoclonal Mouse (purified) |
EIA, IF, IH, WB |
43-45 kD band, astrocytes, intermediate filaments | Human | |
| GFAP
(-poly) Ig |
Advanced Immunochem cat# 021223 |
Polyclonal rabbit (lyophilized) |
ELISA, IF, PAP | astrocytes, support cells of brain and spinal cord | Human | |
| GFAP
(-poly) |
Advanced Immunochem cat# aich31223 reference Song, 2002 van Praag, 2002 Kaspar, 2002 Valličres, 2002 |
polyclonal guinea pig (serum) |
ELISA | Human | ||
| GFAP
(EB4) |
Affiniti cat# GA1170 |
Monoclonal Mouse | ||||
| GFAP (-poly) |
Affiniti cat# GA1171 |
Polyclonal Rabbit | ||||
| GFAP (-poly) |
ABR | immunogen = cytoskeletal preparation of human spinal cord | Polyclonal Rabbit (affinity purified, PBS, azide) |
IH, WB |
GFAP in astrocytes and Schwann cells | Human, mouse, rat |
| GFAP (GF-01) Ig |
ANAWA cat# 4650-0280 |
monoclonal (Ig fract) |
IH(P) | |||
| GFAP
(-poly) |
ANAWA cat# 4650-0100 |
monoclonal (prediluted) | IH(P) | |||
| GFAP
(-poly) |
ANAWA | Polyclonal rabbit (prediluted) |
IH(P) | |||
| GFAP
(-poly) |
ANAWA cat# 4650-0204 |
polyclonal rabbit (serum) |
IH | |||
| GFAP
(-poly) |
ANAWA cat# 4650-0254 |
polyclonal rabbit (serum) |
IH, ELISA | |||
| Antigen (Clone) IgG |
Distributor (Producer) |
Epitope (Immunogen) |
Host (Formulation) |
Methods | Specificity | Reactivity |
| GFAP
(-poly) |
American Qualex cat# M0370 |
monoclonal | ||||
| GFAP
(-poly) |
American Qualex cat# R2090 |
polyclonal | ||||
| GFAP
(6F2) IgG2a |
ARP cat# 03-10505 |
Monoclonal Mouse (cul. sup.) |
IC, IH(F,P),
WB |
Human,
mouse |
||
| GFAP
(6F2) IgG2b |
ARP cat# 05-50160 |
Monoclonal Mouse (purified) |
ELISA, IF,
IH, WB |
Human | ||
| GFAP
(GF12.24) IgG2a |
ARP
cat# 03-61011, purified; cat# 03-65011, prediluted |
monoclonal
(purified or prediluted) |
IC, IH(F,P),
WB |
Human, bov,
rat, mouse |
||
| GFAP
(-poly) |
ARP cat# 03-10555 |
polyclonal
rabbit (serum) |
IC, IH(F,P),
WB |
Human, bov,
rat |
||
| GFAP
(-poly) |
Autogen Bioclear cat# M004 |
monoclonal | ||||
| GFAP
(-poly) |
Autogen Bioclear cat# SC6170 |
polyclonal | ||||
| Antigen (Clone) IgG |
Distributor (Producer) |
Epitope (Immunogen) |
Host (Formulation) |
Methods | Specificity | Reactivity |
| GFAP
(131-17719) IgM |
Biodesign cat# M53027M |
monoclonal
mouse (pur., azide) |
IH | astrocytes and glial related tumors | ||
| GFAP
(GF12.24) IgG2a |
Biodesign cat# M54411M |
monoclonal mouse (lyophilized) |
IH(P),
WB |
50kD GFAP in human brain sections | Human,
bovine, rat, mouse |
|
| GFAP
(GF-01) IgG1 |
Biodesign cat# M61312M |
monoclonal
mouse (azide) |
IH(F,P) | 50kD GFAP in astrocytes, ependymal cells, folliculo- stellate cells | Human, porcine | |
| GFAP
(6F2) IgG1 |
Biodesign cat# M61203M |
monoclonal
mouse (azide, supernatant) |
IF,
IH(F,P) |
56kD GFAP band
in crude brain extracts |
Human,
mouse |
|
| GFAP
(-poly) |
Biodesign cat# M90094S |
23 amino-acid synthetic peptide selected from the amino-acid sequence of GFAP | polyclonal
sheep (affinity purified) |
IH(P) | Human | |
| GFAP
(-poly) |
Biogenesis cat# 4650-0100 |
Bovine spnal cord | polyclonal
rabbit (azide, dilute IgG) |
IH(F,P) | reacts strongly with human GFAP | Human |
| Antigen (Clone) IgG |
Distributor (Producer) |
Epitope (Immunogen) |
Host (Formulation) |
Methods | Specificity | Reactivity |
| GFAP
(-poly) |
Biogenesis cat# 4650-0204 |
Homogeneous GFAP isolated from bovine spinal cords | polyclonal
rabbit (azide, dilute IgG) |
IB,
IH(P) |
monospecific to GFAP in astrocytes | human, bovine, rat, dog, cat and mouse. |
| GFAP
(-poly) |
Biogenesis cat# 4650-0254 |
GFAP from chicken spinal cord | polyclonal
rabbit (azide, dilute IgG) |
IB,
IH(F) |
In IB, GFAP and no other intermediate filament subunits | chicken, mouse, rat and human brain |
| GFAP (-mono) IgG1 |
Biogenesis cat# 4650-0280 |
GFAP isolated from human brain. | monoclonal mouse (azide, diluted IgG) | IH(F,P) | In IB, 56 kD GFAP band. Astrocytes in reactive and neoplastic conditions. | |
| GFAP
(GF-05) IgG1 |
Biogenesis cat# 4650-0309 |
monoclonal
mouse (predilute) |
ELISA, IB,
IH(P?) |
38-45K neurofilaments in immunoblots | may react with GFAP from other species | |
| GFAP
(GA5) IgG1 |
Biogenex cat# 020M reference Lewis, 2001 |
GFAP isolated from porcine spinal cord | monoclonal
mouse (azide, diluted ) |
IH(P) | stains Bergmann glia and astrocytes, localizes specifically GFAP | Human |
| GFAP
(-poly) |
Biogenex cat# 020P |
GFAP isolated from bovine spinal cord. | polyclonal
rabbit (azide, IgG fract.) |
IH(P) | astrocytes and Bergmann glia | many species including human, mouse, and rat |
| Antigen (Clone) IgG |
Distributor (Producer) |
Epitope (Immunogen) |
Host (Formulation) |
Methods | Specificity | Reactivity |
| GFAP
(-poly) |
Biomeda cat# 209M |
Bovine spinal cord. | polyclonal
rabbit (pur., prediluted.) |
IH(F,P) | stains astrocytes, schwann cells, satellite cells and enteric glial cells | Human |
| GFAP
(-poly) |
Biomeda cat# A51 |
polyclonal
rabbit (azide, serum.) |
IH | glial fibrillary acidic protein. | ||
| GFAP
(6F2) IgG1k |
Biomeda cat# 020D |
Glial fibrillary acidic protein isolated from human brain | monoclonal
mouse (predilute) |
IB,
IH(F,P) |
In IB, 52 kD GFAP band. Astrocytes, CNS ependymal cells, astrocytomas and ependymomas | Human, mouse, rabbit |
| GFAP
(6F2) IgG1k |
Biomeda cat# K085 |
Glial Fibrillary Acidic Protein from human brain | monoclonal
mouse (azide) |
IH(F,P) | 52 kD intermediate filament protein GFAP | Human |
| GFAP
(-poly) |
Biomeda cat# N076 |
Bovine spinal cord. | polyclonal
rabbit (azide, serum.) |
IH(F,P) | Astrocytes, ependymal cells, Schwann cells, satellite cells and enteric glial cells | |
| GFAP
(6F2) IgG1k |
Biomeda cat# V1058 |
GFAP isolated from human brain | monoclonal
mouse (prediluted) |
IB,
IH(F,P) |
specific against GFAP only | Human, mouse, rabbit |
| GFAP
(-poly) |
Biomeda cat# V2023 |
Bovine spinal cord. | polyclonal
rabbit (azide.) |
IH(F,P) | stains astrocytes, schwann cells, satellite cells and enteric glial cells | |
| GFAP
(MIG-G2) IgG1k |
Biosource | Purified glial fibrillary acidic protein | monoclonal
mouse (azide, supernatant) |
IH(F,P) | human, guinea pig, and rat | |
| Antigen (Clone) IgG |
Distributor (Producer) |
Epitope (Immunogen) |
Host (Formulation) |
Methods | Specificity | Reactivity |
| GFAP
(-poly) |
Biotrend | Bovine spinal cord | polyclonal
rabbit (azide, prediluted.) |
IH(F,P) | reacts strongly with human GFAP | Human |
| GFAP
(-poly) |
Biotrend | Homogeneous GFAP isolated from bovine spinal cords | polyclonal
rabbit (azide, serum.) |
IB,
IH(P) |
in rat brain cerebellum stains only astrocytes | human, bovine, rat, dog, cat and mouse |
| GFAP
(-poly) IgG1 |
Biotrend | GFAP isolated from human brain | monoclonal
mouse (azide, prediluted) |
IH(F,P) | 56 kD GFAP band.
astrocytes in reactive and neoplastic conditions |
|
| GFAP
(-poly) IgG1 |
Biotrend | purified GFAP from human brain | monoclonal
mouse (azide, Ig fract.) |
ELISA,
IB, IH |
38-45K neurofilaments in immunoblots | may react with other GFAP from other species |
| GFAP
(6F2) IgG1k |
Biotrend | glial fibrillary protein from human brain. | monoclonal mouse (prediluted) |
IH(F,P) | 52 kD intermediate filament protein GFAP in brain and spinal cord | |
| GFAP
(-poly) |
Biotrend | glial fibrillary protein isolated from cow spinal cord | polyclonal
rabbit (azide, Ig fract..) |
IH | astrocytes, ependymal cells, Schwann cells, satellite cells and enteric glial cells | cat, chicken, cow, guinea pig, hamster, monkey, rat, mouse, sheep, kangaroo |
| GFAP
(SMI 26) |
Biotrend cat# GA 2114 |
monoclonal
mouse |
||||
| GFAP
(-poly) |
Biotrend cat# GA-1171, GA-1171B |
polyclonal
rabbit (serum) |
IH,
WB |
|||
| GFAP
(SMI 25) |
Biotrend cat# GA 2112 |
monoclonal
mouse |
||||
| GFAP
(SMI 26) |
Biotrend cat# GA 2106 |
cocktail | monoclonal mouse | |||
| Antigen (Clone) IgG |
Distributor (Producer) |
Epitope (Immunogen) |
Host (Formulation) |
Methods | Specificity | Reactivity |
| GFAP
(SMI 24) |
Biotrend cat# GA 2110 |
monoclonal mouse | ||||
| GFAP
(DB 9) IgG1 |
Biotrend cat# GA 1170, GA 1170A, GA 1170B |
monoclonal mouse
(ascites) |
IH, WB | Human | ||
| GFAP
(6F2) IgG1k |
Biotrend cat# PDM078 |
monoclonal
mouse (predil., supernatant) |
IH | Human | ||
| GFAP
(-poly) IgG1k |
Biotrend cat# PDR028 |
polyclonal
rabbit (predilute, supernatant) |
IH | Human | ||
| GFAP
(GA4) IgG1k |
Biotrend cat# MOB684 |
monoclonal
mouse (supernatant) |
IH | Human | ||
| GFAP
(6F2) IgG1k |
Biotrend cat# MOB199 |
monoclonal mouse (supernatant) | Human | |||
| GFAP
(GF5) IgG2b |
Biotrend cat# 4G25 |
monoclonal
mouse (Ig fract.) |
EIA,IF,
IH,WB |
Human | ||
| GFAP
(-poly) |
BTI cat# BT-575 |
homogeneous GFAP isolated from bovine spinal cords | polyclonal
rabbit (serum) |
IH(P),
WB |
GFAP | human, bovine, rat, dog, cat and mouse |
| GFAP
(G-A-5) IgG1 |
Boehringer Mannheim Cat# 814369 reference Seri, 2001 |
purified glial filament | monoclonal mouse
(lyophilized) |
IC,
IH(F,P) |
astrocytes and Bergman glia cells | human, rat, chicken, pig |
| GFAP (-mono) IgG2a |
CalBiochem cat# 345860 |
Bovine GFAP | monoclonal rat,
(supernatant, sodium azide) |
ELISA, IB,
IH, IP |
56 kDa GFAP, cytoplasm | Human, bovine, rodent |
| Antigen (Clone) IgG |
Distributor (Producer) |
Epitope (Immunogen) |
Host (Formulation) |
Methods | Specificity | Reactivity |
| GFAP
(6F2) IgG1 |
Caltag cat# MON3002 |
monoclonal mouse (azide,
supernatant) |
IB,
IH(F,P) |
cells containing GFAP.
56 kDa band in IB |
Human,
mouse |
|
| GFAP
(GF-01) IgG1 |
Caltag cat# MON3012 |
monoclonal mouse (azide) | IH(F,P) | astrocytes and to a lesser degree ependymal cells, folliculo- stellate cells | Human,
porkine |
|
| GFAP
(-poly) IgG1 |
Caltag cat# PS032 |
human spinal cord.
antigen location, cytoplasm |
polyclonal rabbit
(serum) |
IH(F,P) | Human,
bovine, rat |
|
| GFAP
(-poly) IgG1 |
ICN Cappel cat# 10555 |
polyclonal rabbit | ELISA, IB,
IH(F,P) |
Human | ||
| GFAP
(6F2) IgG |
ICN Cappel cat# 10505 |
monoclonal
mouse |
ELISA, IB,
IH(F,P) |
Human | ||
| GFAP
(6F2) IgG1 |
Capricorn cat# GFP055-48205 |
monoclonal | IB,
ELISA |
IB with 38-45K neurofilaments | Human | |
| GFAP
(GA5) IgG1 |
Cellmarque | monoclonal mouse (ascites, purified, BSA, PBS, azide) |
IH(F,P) | GFAP in astrocytes, Schwann cells, satellite cells, enteric glial cells and some groups of ependymal cells | ||
| GFAP
(-poly) |
Chemicon Cat# AB1540 |
Human GFAP purified from brain | polyclonal
guinea pig (neat, azide) |
ELISA | ||
| GFAP
(-poly) |
Chemicon Cat# AB1980 reference (rabbit GFAP) Wu, 2002 Valličres, 2002 |
Human degraded glial fibrillary acidic protein | polyclonal rabbit (neat, azide) |
ELISA, IB, IPA, RIA | ~50 kDa band, glial cells of the nervous system, glial tumoral cells in IC | human, rat, gerbil, mouse, rabbit, equine and bovine |
| GFAP
(-poly) |
Chemicon Cat# IHC20786 |
immunogen = GFAP isolated from bovine spinal cord | polyclonal rabbit (serum, Ig fract, PBS, Tween, ProClin; predilute) |
IH(P), diagnosis |
GFAP in astrocytes and microglia in the brain and in peripheral nerves; cytoplasmic | human, cat, chicken, cow, guinea pig, hamster, kangaroo, monkey, mouse, rat, sheep |
| GFAP
(-poly) IgG1 |
Chemicon Cat# AB5040 |
polyclonal rabbit (serum, azide) |
ELISA, IIF, IPA |
astrocytes; support (non-nerve) cells of brain and spinal cord | human, porcine, rat, mouse, rabbit. | |
| GFAP
(-poly) |
Chemicon Cat# AB5804 |
immunogen = purified bovine GFAP | polyclonal rabbit (serum, no preserve) |
IC, IH |
stains astrocytes and microglia in the brain, and stains peripheral nerves | human, bovine, dog, rat; others not tested |
| Antigen (Clone) IgG |
Distributor (Producer) |
Epitope (Immunogen) |
Host (Formulation) |
Methods | Specificity | Reactivity |
| GFAP (-mono) |
Chemicon cat# MAB1604 reference (mono GFAP) Van Uden, 2002 |
HUT 78 T-cell line. | Monoclonal Mouse (ascites) |
IH(F) | Reacts with neuroblastoma but not rhabdomyo- sarcoma cells | |
| GFAP
(GA5) IgG1 |
Chemicon Cat# IHC20796 |
immunogen = GFAP isolated from porcine spinal cord | Monoclonal Mouse (predilute) |
IH(P), diagnosis |
GFAP in astrocytes and microglia in the brain and in peripheral nerves; cytoplasmic | human, mammalian |
| GFAP
(G-A-5) IgG1 |
Chemicon cat# MAB3402 |
Purified glial filament | monoclonal
mouse (Ig, purified, lyophilized) |
IC,
IH |
In tissue sections stains astrocytes and Bergman glia cells | human, pig, chicken and rat |
| GFAP (-mono) IgG1 |
Chemicon cat# MAB360 |
Purified GFAP from porcine spinal cord. | monoclonal mouse
(ascites, azide) |
IB, IC,
IH(P) |
~50 kDa band; astrocytes and Bergmann glia cells, glioma and glial cell derived tumors | Human, rat and porcine |
| GFAP
(-poly) |
Cortex cat# CR8013R |
polyclonal
rabbit (neat) |
||||
| GFAP
(DP46-10) IgG1 |
Coulter cat# IM1061 |
monoclonal mouse
(purified) |
IH | 52 kDa band; astrocytes and some ependymal cells | Human | |
| GFAP
(GF12-24) IgG2a |
Cymbus, cat# CBL411 distributed by Chemicon |
Monoclonal Mouse (affinity purified, lyophilized) |
IH(P) WB, diagnosis |
50 kDa GFAP in human brain sections; no cross with other intermediate filaments | Human, bovine, rat, mouse; others not tested | |
| GFAP
(-poly) |
Cyto Skeleton cat# AGF01-A, AGF01-B |
polyclonal
goat (affinity purified) |
IH,
WB |
Human | ||
| GFAP
(-poly) |
Dako cat# N1506 reference (poly -human): Fukumoto 2002 Song, 2002 (poly -bovine) Mastrianni, 2001 |
GFAP from bovine spinal cord | Polyclonal rabbit (liquid, Tris, carrier, azide, ready-to-use) |
IH(P), diagnosis |
GFAP in astrocytes and some ependymal cells of the brain and spinal cord; cytoplasmic; confirmation of glioma in unusual clinical sites | Human, cow |
| GFAP
(-poly) |
Dako cat# H0083 reference (poly -mouse) Sigurdsson 2001 Giasson, 2002 Klein 2002 Lazerov 2002 |
GFAP isolated from cow spinal cord | Polyclonal rabbit (liquid, Ig fract, Tris, stabilizer, ready-to-use) |
IH(F,P), diagnosis |
GFAP; astrocytes in the brain, follicular-stellate cells of the pituitary gland, Schwann cells, and enteric glia cells; cytoplasmic | Human, cow, cat, dog, mouse, rat, sheep |
| GFAP
(-poly) |
Dako cat# Z0334 |
GFAP isolated from cow spinal cord | Polyclonal rabbit (serum, Ig fract, azide) |
IC(F,P), diagnosis |
astrocytes, ependymal cells, Schwann cells, satellite cells and enteric glial cells; cytoplasmic | Human, cat, chicken, cow, guinea pig, hamster, kangaroo, monkey, mouse, rat, sheep |
| Antigen (Clone) IgG |
Distributor (Producer) |
Epitope (Immunogen) |
Host (Formulation) |
Methods | Specificity | Reactivity |
| GFAP
(6F2) IgG1k |
Dako cat# M0761 reference (mono -mouse): Cherny 2001 |
immunogen = GFAP from human brain | Monoclonal Mouse (liquid, supernat, Tris, azide) |
IH(F,P), diagnosis |
52kDa intermediate filament protein GFAP in brain and spinal cord, not other intermediate filament proteins | Human, mouse, rabbit |
| GFAP
(6F2) |
Dako cat# U7038 |
immunogen = GFAP from human brain | Monoclonal Mouse (liquid, Tris, stabilizer, preserve) |
IH(F,P) | 52kDa intermediate filament protein GFAP in brain and spinal cord, not other intermediate filament proteins; astrocytes and some CNS ependymal cells | Human, mouse, rabbit |
| GFAP
(GA5) IgG1 |
Diagnostic Biosys cat# Mob064 |
monoclonal
(concentrated) |
IH | |||
| GFAP
(GA5) IgG1 |
Diagnostic Biosys cat# PDM008 |
monoclonal
(prediluted) |
IH | |||
| GFAP
(6F2) IgG1 |
Diagnostic Biosys cat# Mob199 |
monoclonal
(concentrated) |
IH | |||
| GFAP
(6F2) IgG1 |
Diagnostic Biosys cat# PDM078 |
monoclonal
(prediluted) |
IH | |||
| GFAP
(-poly) |
Diagnostic Biosys cat# RP014 |
polyclonal
rabbit (concentrated) |
IH | |||
| GFAP
(-poly) |
Diagnostic Biosys cat# PDR028 |
polyclonal
rabbit (prediluted) |
||||
| GFAP
(G-A-5) |
Diasorin cat# 22252 |
GFAP isolated from bovine spinal cord. | polyclonal rabbit (prediluted) |
IF, IH |
||
| GFAP
(2A5) IgG1k |
EnCor Biotech cat# MCA2A5 |
extensively purified GFAP extracted from pig spinal cord | Monoclonal Mouse (concentrated tissue culture supernatant) |
IF, WB |
GFAP | Human, cow, pig, mouse, rat, all other mammals, avian |
| GFAP
(-poly) Ig |
EnCor Biotech cat# RPCA-GFAP |
GFAP purified from Triton X-100 extract of myelin associated material from bovine spinal cord | Polyclonal rabbit (crude serum, high titre) |
IC, WB |
GFAP on cells in tissue culture or in tissue sections | Human, rat, mouse, cat, other mammals |
| GFAP
(-poly) IgY |
EnCor Biotech cat# CPCA-GFAP |
GFAP purified from Triton X-100 extract of myelin associated material from bovine spinal cord | Polyclonal chicken (affinity purified) |
IF | GFAP | Human, rat, mouse, cat, other mammals |
| GFAP
(GF2) |
Enzo cat# 30934, C34934 |
monoclonal (concentrated or ready-to-use) |
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| GFAP | ICN Pharm. cat# 697021 |
Monoclonal Mouse (liquid) |
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| GFAP
(-poly) |
ICN Pharm. cat# 657851 |
polyclonal
rabbit (liquid IgG fraction) |
Human | |||
| GFAP
(8) IgG1 |
ICN Biomed cat# 691821 |
human spinal cord. | monoclonal mouse
(supernatant) |
ELISA,
IB, IH |
recognizes the
56Kd GFAP protein |
Human |
| Antigen (Clone) IgG |
Distributor (Producer) |
Epitope (Immunogen) |
Host (Formulation) |
Methods | Specificity | Reactivity |
| GFAP
(-poly) IgG1 |
ICN Biomed cat# 691102 |
fusion of mouse myeloma cells and
mouse spleen cells immunized with purified porcine GFAP. |
polyclonal Rabbit | PAP,
IH |
single 51 kDa band.in IB | Porcine, broadly cross-reactive |
| GFAP
(GA-5) IgG1 |
Innogenex cat# AM2185 |
GFAP isolated from porcine spinal cord | monoclonal mouse
(azide) |
IB,
IH |
50kD glial fibrillary acidic protein | Human, pig, rat, chicken |
| GFAP
(-poly) |
Innogenex cat# AP2185 |
GFAP isolated from porcine spinal cord | polyclonal
rabbit (azide) |
IH(P) | Human, mouse, and rat | |
| GFAP | Innovex cat# MAB341 |
monoclonal
mouse |
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| GFAP
(GA-5) IgG1 |
Kamiya cat# MC023 |
Porcine Spinal Cord | monoclonal
mouse (purified, ascites) |
IH,
WB |
51-52 kDa glial fibrillary acidic protein | human, rat, and chicken, others not tested. |
| GFAP (G25-8) IgG |
Kamiya cat# MC020 |
Monoclonal Mouse (PBS, BSA, azide) | IH(P) | GFAP | ||
| GFAP
(-poly) |
Lab Vision cat# RB-087 |
immunogen = GFAP isolated from cow spinal cord | Polyclonal rabbit (serum, PBS, BSA, azide; or ready to use for IH(P)) |
IH(P) | GFAP, cytoplasmic; stains astrocytes, glial cells, ependymal cells, and their tumors; some weak staining of axons in PNS | Human, monkey, cow, sheep, guinea pig, rat, mouse, hamster and chicken; others not tested |
| GFAP
(ASTRO5) IgM |
Lab Vision cat# MS-1180 |
immunogen = purified GFAP | Monoclonal Mouse (ascites, affinity purified, PBS, with or w/o BSA & azide) |
IF, IH(P) |
GFAP, cytoplasmic; stains astrocytes, glial cells, ependymal cells, and their tumors; for differentiating primary gliomas from metastatic lesions | Human; others not tested |
| GFAP
(ASTRO6) IgG1 |
Lab Vision cat# MS-1376 |
immunogen = GFAP | Monoclonal Mouse (ascites, protein G purified, PBS, with or w/o BSA & azide) |
IF, IH(P), WB |
GFAP, cytoplasmic; stains astrocytes, glial cells, ependymal cells, and their tumors; for differentiating primary gliomas from metastatic lesions | Human, pig, rat, chicken; others not tested |
| GFAP
(ASTRO5 & ASTRO6) IgM & IgG1 |
Lab Vision cat# MS-1407 |
immunogen = GFAP | Monoclonal Mouse (ascites, affinity & protein G purified, PBS, with or w/o BSA & azide, or ready to use) |
IF, IH(P) |
GFAP, cytoplasmic; stains astrocytes, glial cells, ependymal cells, and their tumors; for differentiating primary gliomas from metastatic lesions | Human; others not tested |
| GFAP
(GA5) IgG1 |
Lab Vision cat# MS-280 |
immunogen = porcine spinal cord | Monoclonal Mouse (ascites, protein G purified, PBS, with or w/o BSA & azide) |
IF, IH(P) IP, WB |
GFAP, cytoplasmic; stains astrocytes, glial cells, ependymal cells, and their tumors; for differentiating primary gliomas from metastatic lesions | Human; others not tested |
| GFAP
(YC10) IgG1 |
Leinco cat# G138 |
residues 3-13 of GFAP | Monoclonal Mouse (pur., lyophilized) |
IB, IH |
recognizes sequence T-phospho S-A-A-R-R (residues 7-12) of GFAP; not unphospho GFAP or other other phospho intermediate filament proteins | Porcine, Human and Bovine, not mouse or rat |
| GFAP
(-poly) |
Maine
Biotech |
Homogenous GFAP isolated from bovine spinal cord | polyclonal
rabbit (serum, azide) |
IB,
IH |
stains only astrocytes
in IH |
Human, bovine, rat, dog, cat and mouse |
| Antigen (Clone) IgG |
Distributor (Producer) |
Epitope (Immunogen) |
Host (Formulation) |
Methods | Specificity | Reactivity |
| GFAP
(YC10) IgG1 |
MBL
Internatl |
residues 3-13 of GFAP | monoclonal mouse
(lyophilized) |
IB,
IC |
recognizes sequence T-pS- A-A-R-R (residues 7-12 of GFAP) specifically. Only phospho-GFAP | Porcine, Human and Bovine, not mouse or rat |
| GFAP
(6F2) IgG1 |
NovoCastra | Glial fibrillary acidic protein from human brain. | monoclonal mouse (lyophlized, supernat) | IH(F,P) | 52kD GFAP, staining is cytoplasmic | Human, porcine |
| GFAP
(GA5) IgG1 |
NovoCastra | Porcine spnal cord | monoclonal mouse (lyophlized, supernat) | IH(F,P) | 52kD GFAP, staining is cytoplasmic | Human |
| GFAP [R40] (-poly) |
Novus Biologicals Cat# NB300-141 reference Yoo, 2003 |
native GFAP purified from porcine spnal cord | Polyclonal Rabbit (serum, azide) |
IC(P), IF, IP, WB |
mammalian GFAP | Human, rat, mouse, cat, pig, cow, mammals |
| GFAP (-poly) |
Novus Biologicals Cat# ab7779 produced by Abcam (see above) |
immunogen = GFAP from bovine spinal cord | Polyclonal rabbit (liquid, whole serum, Tris, BMC-6, azide) | IH(F,P) | astrocytes in brain; in PNF, Shwann cells, satellite cells, and enteric glial cells | |
| GFAP (-poly) |
Novus Biologicals Cat# ab929 produced by Abcam (see above) |
immunogen = GFAP isolated from cow spinal cord | Polyclonal rabbit (liquid, PBS, azide, predilute) | IH(F,P) | astrocytes and some ependymal cells in brain; in PNF, Shwann cells, satellite cells, and enteric glial cells | Human, cat, chicken, cow, guinea pig, hamster, monkey, rat, mouse, sheep, kangaroo |
| GFAP
(2A5) IgG1 |
Novus Biologicals Cat# NB300-142 |
native GFAP purified from porcine spnal cord | Monoclonal Mouse (liquid, supernat) | ELISA, IF, IH(P), IP, WB |
55kD GFAP, astrocytic cells, their progenitors and descendents | Human, mammals |
| GFAP
(GF-01) IgG1 |
Novus Biologicals Cat# ab7806 produced by Abcam (see above) |
immunogen = pellet of pig brain cold-stable proteins after depolymer- ization of microtubules | Monoclonal Mouse (liquid, PBS) | ELISA, IC, IH(F,P), WB |
GFAP in astrocytes | Human, pig; not rat, mouse |
| GFAP
(6F2) IgG1 |
Novus Biologicals Cat# ab8975 produced by Abcam (see above) |
immunogen = GFAP from human brain | Monoclonal Mouse (liquid, tissue culture supernat, azide) | IH(F,P), WB |
GFAP in astrocytic cells in CNS, Schwann cells in PNS | Human; others not tested |
| GFAP
(GA5) IgG1 |
Novus Biologicals Cat# ab9551 produced by Abcam (see above) |
immunogen = GFAP isolated from porcine spinal cord | Monoclonal Mouse (purified Ig, PBS, preserved) | IH(P) | GFAP in astrocytes, ependymal cells and corresponding tumors; no cross react with other intermediate filaments | Human, pig, rat, chicken; others not tested |
| GFAP (131-17719) IgG1 |
OEM Concepts cat# M2-M27 |
monoclonal mouse | ||||
| GFAP
(YC10) |
Panvera cat# MBL-MY013 |
monoclonal
pig |
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| GFAP
(-poly) |
Progen cat# GP52 |
polyclonal
guinea pig (serum) |
IH(F,P),
WB |
detection of 52 kD GFAP in astrocytomas, gangliomas,
medullo- blastomas,
certain ependymonas, certain teratomas |
human, bovine, rat | |
| GFAP (C9) IgG1k |
Progen cat# 16073 |
monoclonal
mouse (lyophilized) |
IH(F,P) | discrimination of glial tumors, including astrocytomas and ependymonas, | human, rat | |
| GFAP
(GF12.24) IgG2a |
Progen cat# 65011 |
. | monoclonal mouse (predilute, supernat) | IC,
IH(F,P), WB |
Specifically detects glia cells, astrocytes and tumors derived therefrom | human, bovine, rat, mouse |
| GFAP
(GF12.24) IgG2a |
Progen cat# 61011 |
monoclonal
mouse (pur, lyophilized) |
IC,
IH(F,P), WB |
Specifically detects glia cells, astrocytes and tumors derived therefrom | human, bovine, rat, mouse | |
| Antigen (Clone) IgG |
Distributor (Producer) |
Epitope (Immunogen) |
Host (Formulation) |
Methods | Specificity | Reactivity |
| GFAP
(-poly) IgG1 |
Progen cat# 10555 |
polyclonal rabbit (serum) | IC,
IH(F,P), WB |
Stains glia cells, astrocytes and tumors derived therefrom | human, rat, bovine | |
| GFAP
(1B4) IgG2b |
RDI | bovine spinal cord homogenate | monoclonal
mouse |
IH(F,P),
IIF, WB |
GFAP in brain homogenates | human, mouse, rat, bovine, ovine, canine, pig, rabbit, guinea pig and chick |
| GFAP
(2E1) IgG2b |
RDI | bovine spinal cord homogenate | monoclonal
mouse |
IH(F,P),
IIF, WB |
GFAP in brain homogenates | human, mouse, rat, bovine, ovine, canine, pig, rabbit, guinea pig and chick |
| GFAP
(4A11) IgG2b |
RDI | bovine spinal cord homogenate | monoclonal
mouse |
IH(F,P),
IIF, WB |
GFAP in brain homogenates | human, mouse, rat, bovine, ovine, canine, pig, rabbit, guinea pig and chick |
| GFAP
(1B4,2E1, 4A11) IgG2b |
RDI | bovine spinal cord homogenate | monoclonal mouse (cocktail) | IH(F,P),
IIF, WB |
GFAP in brain homogenates | human, mouse, rat, bovine, ovine, canine, pig, rabbit, guinea pig and chick |
| GFAP
(-poly) IgG |
RDI | epitope corresponding to amino acids 413-431 mapping at the carboxy terminus of human GFAP | polyclonal
goat (azide) |
IH,
WB |
GFAP, non cross reactive with other intermediate filaments | Human, mouse, rat |
| Antigen (Clone) IgG |
Distributor (Producer) |
Epitope (Immunogen) |
Host (Formulation) |
Methods | Specificity | Reactivity |
| GFAP
(-poly) IgG |
RDI | epitope corresponding to amino acids 2-19 mapping at the amino terminus of human GFAP | polyclonal goat
(azide) |
IH,
WB |
GFAP, non cross reactive with other intermediate filaments | Human, |
| GFAP
(-poly) IgG |
RDI | GFAP isolated from spinal cord | polyclonal
rabbit (azide) |
IB,
IH(F,P) |
56 kDa GFAP protein | Human, cow, rat |
| GFAP
(GF12.24) IgG2a |
RDI | Intermediate filament cytoskeleton from cultured human glioma cells | monoclonal mouse (Protein-A, recombinant, lyophilized) |
IB, IH(F,P) |
marker for cell typing; prenatal diagnosis of neural tube defects | human, bovine, rat and mouse |
| GFAP
(GF5) IgG2b |
RDI | Purified glial fibrillary acidic protein from human brain | monoclonal
mouse (azide) |
ELISA,
IIF, IH |
43-45 kD band corr. to GFAP from human brain and spinal cord. | Human |
| GFAP (-poly) IgG |
Santacruz | epitope mapping at the carboxy terminus of human GFAP | polyclonal goat (IgG affinity purified, PBS, azide, gelatin, blocking peptide) |
IH, IP, WB |
GFAP | Human, mouse, rat |
| GFAP (-poly) IgG |
Santacruz | epitope mapping at the amino terminus of human GFAP | polyclonal goat (IgG affinity purified, PBS, azide, gelatin, blocking peptide) |
IH, WB |
GFAP | Human, mouse, rat |
| GFAP (-poly) IgG |
Santacruz | recombinant protein corresponding to amino acids 1-50 mapping at the amino terminus of human GFAP | polyclonal rabbit (IgG, PBS, azide, gelatin, blocking peptide) |
IH, IP WB |
GFAP | Human, mouse, rat |
| GFAP (-mono) IgG1 |
Santacruz | immunogen = full length human GFAP | monoclonal mouse (IgG, PBS, azide, gelatin) |
IH(P), not WB |
GFAP | Human |
| GFAP
(6F2) IgG1k |
Scytek cat# A00013 |
GFAP from human brain | monoclonal
mouse (ready to use) |
IH(F,P) | 52 kD intermediate filament protein GFAP in brain and spinal cord. | Human |
| Antigen (Clone) IgG |
Distributor (Producer) |
Epitope (Immunogen) |
Host (Formulation) |
Methods | Specificity | Reactivity |
| GFAP
(GA5) IgG1k |
Scytek cat# A9064C |
glial fibrillary protein from human brain | monoclonal
mouse (azide, supernatant) |
IH | 52 kD intermediate filament protein GFAP in brain and spinal cord. Labels some astrocytes and some CNS ependymal cells but not oligo dendrocytes or neurons. | |
| GFAP
(-poly) IgG |
Scytek cat# A9114C |
glial fibrillary protein isolated from cow spinal cord | polyclonal rabbit (azide, purified) | IH | GFAP | cat, chicken, cow, guinea pig, hamster, monkey, rat, mouse, sheep, kangaroo |
| GFAP
(DP46.10) IgG1 |
Serotec | Intermediate filament from bovine spinal cord | monoclonal
mouse (azide, supernatant) |
IH(F,P) | a 50kD intermediate filament found only in glial cells or cells of glial origin (eg astrocytes, ependymal cells and Schwann cells). This antibody stains normal glial cells as well as astrocytomas and ependyomas | Human, rabbit, mouse |
| GFAP
(DP46.10) IgG1 |
Serotec | Intermediate filament from bovine spinal cord | monoclonal
mouse (azide, supernatant, ready to use) |
IH,
WB |
a 50kD intermediate filament found only in glial cells or cells of glial origin (eg astrocytes, ependymal cells and Schwann cells). | Human, rabbit, mouse |
| Antigen (Clone) IgG |
Distributor (Producer) |
Epitope (Immunogen) |
Host (Formulation) |
Methods | Specificity | Reactivity |
| GFAP
(G-A-5) IgG1 |
Sigma Aldrich cat# C9205 reference (anti-GFAP) Sun, 2001 |
porcine spinal cord GFAP | monoclonal
mouse (azide, purified) |
IH(P), double labeling | ||
| GFAP
(G-A-5) IgG1 |
Sigma Aldrich cat# G3893 |
porcine spinal cord GFAP | Monoclonal Mouse (azide, purified) |
IB, IIF,
IH(P) |
GFAP; does not cross react with vimentin | human, pig, rat |
| GFAP
(-poly) IgG |
Sigma Aldrich cat# G9269 reference Götz, 2001 |
GFAP from human brain | polyclonal rabbit (azide, IgG fract.) |
IH(P),
IB |
GFAP in astrocytes, glia cells (Bergmann glia), gliomas and other glial cell derived tumors | rat |
| GFAP
(-poly) |
Sigma Aldrich cat# IMMH6 |
polyclonal rabbbit | IH kit | Human | ||
| GFAP
(52) IgG1 |
Transduction is now BD Biosciences
cat# G49220 |
residues GEVIKESK- QEHKDVM, position: 418 - 432 | monoclonal mouse (predilute, supernat) | IF, IH, WB | 50 kDa GFAP | Human, rat, mouse, chick |
| GFAP
(-poly) |
Ventana cat# 760-2516 |
polyclonal | Human | |||
| GFAP
(ZCG29) IgG1k |
Zymed | Human brain | monoclonal mouse
(ascites) |
IH | 56 kD GFAP band from immunoblots of crude brain extracts | Human |
| GFAP
(-poly) |
Zymed | Bovine spinal cord | polyclonal
rabbit (purified) |
IH(F,P) | normal and neoplastic astrocytic cells | Human, rat ,
mouse and other animal species |
| GFAP
(2.2B10) IgG2bk |
Zymed | fibrous neuroglia of multiple sclerosis plaques. | monoclonal
rat (purified, unconjugatd) |
ELISA,
IF, IH, WB |
55 kD protein present in astrocytes and Bergmann-glial cells. | Bovine,
human and rodent |
| Antigen (Clone) IgG |
Distributor (Producer) |
Epitope (Immunogen) |
Host (Formulation) |
Methods | Specificity | Reactivity |
