This protocol describes the preparation of primary glial cultures consisting of ~75% astrocytes and ~25% microglia. The protocol specifies neonatal mouse cerebral cortex, but also works for late embryos, for rat, and for most CNS regions. Our protocols for preparing highly enriched astroglial or microglial cultures are also available in this database.
- Saura J. Microglial cells in astroglial cultures: a cautionary note. J Neuroinflammation. 2007;4:26. PubMed.
microglia, astrocytes, glia, mouse, rat, rodent, primary cell culture