This protocol describes the preparation of primary cultures consisting of >95% microglia from neonatal mouse cerebral cortex. It also works for late embryos, for rat and for most CNS regions. Our protocols for preparing mixed glial or highly enriched astroglial cultures are also available in this database.
- Saura J, Tusell JM, Serratosa J. High-yield isolation of murine microglia by mild trypsinization. Glia. 2003 Dec;44(3):183-9. PubMed.
microglia, mouse, rat, rodent, primary cell, glia