Hu X, Crick SL, Bu G, Frieden C, Pappu RV, Lee JM.
Amyloid seeds formed by cellular uptake, concentration, and aggregation of the amyloid-beta peptide.
Proc Natl Acad Sci U S A. 2009 Dec 1;106(48):20324-9.
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This is an interesting study. The ability to visualize intracellular accumulation of Aβ after 24-hour incubation with very low extracellular Aβ (1 nm) is remarkable. The internalization seems to be sequence-specific; this would require a receptor (still unknown) with high affinity to Aβ.
The study also demonstrates that internalized Aβ formed HMW aggregates. Moreover, these aggregates are capable of seeding Aβ fibril formation in vitro. The fate of these aggregates remains uncertain based on this paper. Do they get released back into the media by exocytosis?