Overexpression of low-density lipoprotein receptor in the brain markedly inhibits amyloid deposition and increases extracellular A beta clearance. - AlzForum Alzheimer Research Paper of the Week


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Home: Papers of the Week

Annotation

	Kim J, Castellano JM, Jiang H, Basak JM, Parsadanian M, Pham V, Mason SM, Paul SM, Holtzman DM. Overexpression of low-density lipoprotein receptor in the brain markedly inhibits amyloid deposition and increases extracellular A beta clearance. Neuron. 2009 Dec 10;64(5):632-44. PubMed Abstract

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REAGENTS/MATERIAL:
Sandwich ELISA for Ab and ApoE:
For Ab ELISA, HJ2 (anti-Ab35-40) and HJ7.4 (anti-Ab37-42) were used as capture antibodies and HJ5.1-biotin (anti-Ab13-28) as the detection antibody. Monoclonal anti-human-ApoE (WUE4) and anti-ApoE (Calbiochem) were used for apoE ELISA.
For in vivo microdialysis experiments, human Ab1-x from collected fractions was measured using m266 antibody (anti-Ab13- 28) to capture and 3D6-biotinylated antibody (anti-Ab1-5) to detect.
Quantitative Analyses of Neuroinflammatory Response
Brain sections cut with a freezing sliding microtome were immunostained with anti-CD11b antibody (BD Pharmingen), anti-CD45 (Serotec), and anti-GFAP (Chemicon).
Western Blot Blots were probed for LDLR with antibodies from Novus, Abcam, and a gift from Dr. Guojun Bu at Washington University. Blots were also probed for CT22 (Zymed), HA (Covance) and ApoJ (Covance).