Fagan AM, Mintun MA, Shah AR, Aldea P, Roe CM, Mach RH, Marcus D, Morris JC, Holtzman DM. Cerebrospinal fluid tau and ptau181 increase with cortical amyloid deposition in cognitively normal individuals: Implications for future clinical trials of Alzheimer's disease EMBO Molecular Medicine. 2009 Nov 1;1(8-9):371-380. Abstract

REAGENTS/MATERIAL:
CSF samples were analysed for Ab42, total tau and phospho-tau181 (ptau181) by enzyme-linked immunosorbant assay (ELISA) (InnotestTM, Innogenetics, Ghent, Belgium). CSF Ab40 was assayed by ELISA (Cirrito et al, 2003), and plasma Ab1–40, Ab1–42 and Abx–40, Abx–42 were analysed by xMAP (bead-based) ELISA (Inno-Bia Plasma Ab Forms Multiplex AssayTM; Innogenetics, Ghent, Belgium). CSF Ab38 was analysed by an antibody-based electrochemiluminescence assay according to the manufacturer’s recommendations (MSD 96 well Multi-Array Human (6E10) Abeta 38 Ultra-Sensitive KitTM, Meso Scale Discovery, Gaithersburg, MD)