Liu B, Rasool S, Yang Z, Glabe CG, Schreiber SS, Ge J, Tan Z. Amyloid-peptide vaccinations reduce {beta}-amyloid plaques but exacerbate vascular deposition and inflammation in the retina of Alzheimer's transgenic mice. Am J Pathol. 2009 Nov;175(5):2099-110. PubMed Abstract

REAGENTS/MATERIAL:
Primary Antibodies used in this study: rabbit anti-human APP (Affinity Bioreagents, Golden, CO); mouse monoclonal anti-Aβ (BAM01 or 6F/3D) cross-reacts with APP (Neomarkers, Fremont, CA); mouse monoclonal anti-Aβ (6E10) cross-reacts with APP (Covance Research Products, Denver, PA); mouse monoclonal anti-Aβ42 (12F4) (Signet Covance); mouse monoclonal anti-Aβ40 (5C3) (Assay Designs Stressgen, Ann Arbor, MI); mouse monoclonal anti-GFAP (GA5) a marker for astrocytes or Müller cells (Sigma-Aldrich); rabbit anti-IBA1, a marker for microglia (Biocare Medical, Concord, CA); mouse monoclonal anti-hyperphosphorylated tau (AT8) (Pierce Thermo Scientific, Rockford, IL) and rabbit anti-von Willebrand factor (Sigma-Aldrich)