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REAGENTS/MATERIAL:
Anti-ceNup35 (NPP-19), anti-ceNup107 (NPP -5) and anti-ceNup153 (NPP -7) were
kindly provided by P. Askjaer (Galy et al., 2003).
Anti-Ndc1 and anti-Nup153 antibodies were a gift of U. Kutay (Mansfeld et al., 2006)
and B. Burke (Bodoor et al., 1999).
Anti-Nup107 used for immunofluorescence studies was a gift of V. Doye (Belgareh et al., 2001).
Anti-Lap2β was a gift from R. Foisner.
The following antibodies were purchased:
monoclonal mouse anti-myosin Heavy Chain (MF-20) (DSHB, University of Iowa); 31300,
monoclonal mouse anti-GAPDH (6C5) (Abcam); 25781
anti-H2B (Upstate);
monoclonal mouse anti-tubulin (B512) (Sigma Aldrich); 9336
rabbit anti-Lamin A (Sigma Aldrich); 31318 .
anti-tubulin β III (Covance);
monoclonal mouse anti NPC proteins (mAb414) 31337 (Covance) and
monoclonal mouse anti-Nup93 (3332 C2a) 31338 (Santa Cruz).
Anti-ceNup160 (NPP -6) and mammalian Nup43, Sec13 and Nup107, used for western
blot and immunoprecipitation studies, were generated by injecting rabbits with GSTceNup160
(aa1183-1586), His-hSec13, MBP-hNup43, and His-mNup107 (aa. 600-926) recombinant proteins.
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