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Paquet D, Bhat R, Sydow A, Mandelkow EM, Berg S, Hellberg S, Fälting J, Distel M, Köster RW, Schmid B, Haass C.
A zebrafish model of tauopathy allows in vivo imaging of neuronal cell death and drug evaluation. J Clin Invest.
2009 May;119(5):1382-95.
PubMed Abstract
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REAGENTS/MATERIAL:
The following antibodies/kits were used in this paper for IF, immunohistochemistry (IHC), and WB:
monoclonal mouse anti-Tau, C-terminal region (T46) IF, WB (Zymed Invitrogen);
rabbit anti-Tau, 4-repeat section (K9JA) IF, WB (DAKO);
anti-Tau (DA9) IHC (P. Davies, Albert Einstein College of Medicine, NY, USA);
monoclonal mouse anti-PHF-Tau, phosphorylated at Ser202 (AT8) IF, IHC, WB (Pierce, Thermo Scientific);
monoclonal mouse anti-Tau, phosphorylated at Ser422 (422) IF, WB (C. Czech, Hoffmann La Roche, Basel, Switzerland);
monoclonal mouse anti-PHF-Tau, phosphorylated at Thr231 (AT180) IF, WB (Pierce, Thermo Scientific);
monoclonal mouse anti-Tau, phosphorylated at Ser262/356 (12E8) IF, WB (P. Seubert, Elan Pharmaceuticals, San Francisco, CA, USA);
monoclonal mouse anti-Tau (MC1) IF (P. Davies);
monoclonal mouse anti-Tau, phosphorylated at Ser396/404 (PHF1) IF, WB (P. Davies);
monoclonal mouse anti-synaptotagmin (znp-1) IHC (Developmental Studies Hybridoma Bank, U IOWA);
for WB, rabbit anti-tau, phosphorylated at Serine 396 (pS396) and monoclonal mouse anti-tau (Tau5)
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