Chu J, Hong NA, Masuda CA, Jenkins BV, Nelms KA, Goodnow CC, Glynne RJ, Wu H, Masliah E, Joazeiro CA, Kay SA.
A mouse forward genetics screen identifies LISTERIN as an E3 ubiquitin ligase involved in neurodegeneration.Proc Natl Acad Sci U S A.
2009 Feb 17;106(7):2097-103.
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REAGENTS/MATERIAL: Antibodies: Immunohistochemistry: Primary antibodies used were rabbit anti-GFAP (Dako)
and monoclonal mouse anti-nonphospho-neurofilament (SMI32) (Sternberger Monoclonals Inc.). Protein Extraction and Western Blot Analyses: Primary bodies used:
monoclonal mouse anti-tau-5 (Biosource Invitrogen),
monoclonal mouse anti-human PHF phospho-tau, (AT8) (Autogen bioclear) and
monoclonal mouse anti-human PHF phospho-tau (AT180) (Autogen bioclear).
A monoclonal Lister antibody (clone5L11) raised against a N-terminally truncated version of human LISTERIN (starting from N713) was used.