Specific SIRT1 activation mimics low energy levels and protects against diet-induced metabolic disorders by enhancing fat oxidation. - AlzForum Alzheimer Research Paper of the Week


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Annotation

	Feige JN, Lagouge M, Canto C, Strehle A, Houten SM, Milne JC, Lambert PD, Mataki C, Elliott PJ, Auwerx J. Specific SIRT1 activation mimics low energy levels and protects against diet-induced metabolic disorders by enhancing fat oxidation. Cell Metab. 2008 Nov;8(5):347-58. PubMed Abstract

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REAGENTS/MATERIAL:
Western blotting:
monoclonal mouse anti-total-AMPK (F6) (Cell Signaling); rabbit anti-phosphorylated AMPKα(Cell Signaling); rabbit anti-phosphorylated ACC (Cell Signaling); anti-p53(Cell Signaling); rabbit anti-acetylated p53 (Cell Signaling)
Immunoprecipitation
monoclonal rabbit anti-PGC-1a (3G6) (Cell Signaling) and monoclonal rabbit anti-FOXO1 (L27) (Cell Signaling)




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