Neuropeptide Y fragments derived from neprilysin processing are neuroprotective in a transgenic model of Alzheimer's disease. - AlzForum Alzheimer Research Paper of the Week


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Home: Papers of the Week

Annotation

	Rose JB, Crews L, Rockenstein E, Adame A, Mante M, Hersh LB, Gage FH, Spencer B, Potkar R, Marr RA, Masliah E. Neuropeptide Y fragments derived from neprilysin processing are neuroprotective in a transgenic model of Alzheimer's disease. J Neurosci. 2009 Jan 28;29(4):1115-25. PubMed Abstract

	Comments on Paper and Primary News

	Primary News: Peptide Brace Against AD—Insulin, Neuropeptide Y Tame Aβ Toxicity Comment by: Tony Turner
	Submitted 17 February 2009 \| Permalink	Posted 2 March 2009
	The comment that the cleavage of neuropeptide Y to generate a biologically active fragment by neprilysin (Neutral EndoPeptidase-24.11) is the first such example for the enzyme is incorrect. At least one example has previously been reported in the metabolism of calcitonin gene-related peptide (CGRP) (Davies et al., 1992). References: Davies D, Medeiros MS, Keen J, Turner AJ, Haynes LW. Endopeptidase-24.11 cleaves a chemotactic factor from alpha-calcitonin gene-related peptide. Biochem Pharmacol. 1992 Apr 15;43(8):1753-6. Abstract View all comments by Tony Turner

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REAGENTS/MATERIAL:
Antibodies used in this study are:
Immunocytochemical analysis of neurodegeneration: monoclonal mouse anti-synaptophysin (Millipore Bioscience); monoclonal mouse anti-MAP2 (AP20) (Millipore Bioscience); monoclonal mouse anti-NeuN (A60) (Millipore Bioscience) or monoclonal mouse anti-GFAP (GA5) (Millipore Bioscience).
Western Bloting analysis: monoclonal mouse anti-FL APP (22C11) (Millipore Bioscience); monoclonal mouse anti-human APP (8E5) (Elan Pharmaceuticals); monoclonal mouse anti-Amyloid β (6E10) (Signet Laboratories); monoclonal mouse anti-NEP (Abcam); monoclonal mouse anti-BDNF (35928.11) (Calbiochem); monoclonal mouse anti-NGF (25623.1) (Calbiochem, discontinued); polyclonal anti-Neurotropin-3 (Promega); monoclonal mouse anti-Neurotropin-4 (36507) (R&D Systems) and rabbit anti-Neuropeptide Y (Peninsula Laboratories Bachem)
Determination of Aβ, SP, ENK, and NPY levels by ELISA: ELISA kits for NPY (Phoenix Pharmaceuticals); Substance P (Assay Designs) and Met-ENK (Peninsula Laboratories Bachem)
Analysis of FL-NPY and NPY CTFs by immunoblot and immunocytochemistry: rabbit polyclonal anti-FL-NPY (Peninsula Laboratories); an antibody against NPY CTFs that was prepared at Invitrogen by immunizing rabbits with the peptide corresponding to the amidated 31–36 aa of NPY with an extra Cys added to the N-terminus for conjugation, and rabbit polyclonal anti-NPY CTFs (20 –36) (Santa Cruz).
Double immunocytochemical and laser-scanning confocal analysis: monoclonal anti-NEP (Abcam); monoclonal mouse anti-APP (8E5) (Elan Pharmaceuticals) or rabbit polyclonal antibodies against FL-NPY (Peninsula Laboratories).
Lentivirus NEP preparation: Flow cytometry using a monoclonal mouse anti-NEP (56C6) (Research Diagnostics Fitzgerald)