 |
|
|
|
| |
Submit a Comment on this Paper |
|
|
|
|
| |
 |
|
| |
REAGENTS/MATERIAL:
The primary antibodies used were:
monoclonal mouse anti-Dcp1a (Abcam);
rabbit anti-Dcp1 serum (kindly provided by B. Séraphin, CNRS, Gif-sur-Yvette), raised against a recombinant human Dcp1a;
goat anti-PSD95 antibody (Santa Cruz);
rabbit anti-Ago2, raised against a recombinant rat Ago2 protein fragment, detects all four mammalian Ago proteins (from T. Hobman, University of Alberta, Edmonton, Alberta, Canada);
rabbit anti-RCK/p54 (Bethyl Laboratory);
anti-rRNA monoclonal Y10B (from J. Steitz, Yale University Medical School, New Haven, CT);
anti-ZBP1 antibodies (from the laboratory of R. H. Singer, Albert Einstein College of Medicine, NY) and
anti-TAU1 antibody (gift from V. Homburger, IGF, Montpellier, France).
The autoimmune serum against human GW182 protein (18033; gift from M. J. Fritzler, University of Calgary, Calgary, Alberta, Canada) was used for detection of GW182 and
was used as the source of primary antibody against GW182 to stain the P-body-like structures.
|
|
|
|
Home
