Conti L, Sipione S, Magrassi L, Bonfanti L, Rigamonti D, Pettirossi V, Peschanski M, Haddad B, Pelicci P, Milanesi G, Pelicci G, Cattaneo E. Shc signaling in differentiating neural progenitor cells. Nat Neurosci. 2001 Jun;4(6):579-86. PubMed Abstract

REAGENTS/MATERIAL:
Human telencephalic primary cells were isolated from fetuses at 8-9 weeks after conception. ST14A cells which carry Shc LY294002 and wortmannin were applied at 10 uM and 5 uM, respectively for inhibition experiments. Antiserum against ShcC, 1:100 dilution, commercially available (Transduction Laboratories) Anti MAP2, 1:200 dilution (Roche) Anti-bIII-Tubulin, 1:200 dilution (Promega) Anti-NF-68, 1:200 dilution (Transduction Laboratories)/ Anti-GFAP, 1:200 dilution (Sigma) Polyclonal anti-ShcA for immunoprecipitation (Transduction Labratories) Anti-phosphotyrosine polyclonal antibody, 1:2000 dilution (New England Biolabs) Anti-PSD-95 monoclonal antibody (Transduction Laboratories) Anti-Bad, 1:1000 dilution, (New England Biolabs) Anti Erk1, 1:4000 dilution, (Santa Cruz Biotechnology) Anti-Akt, 1:1000 dilution, (New England Biolabs)

FUTURE DIRECTION:
Studies on ShcC levels and activity needed when determining strategies for interfereing with neuronal cell demise in neurodegenerative disease. See Reuters Health story: http://dailynews.yahoo.com/htx/nm/20010522/hl/stemcells_1.html See Scientific American story: http://www.sciam.com/news/052301/2.html