Phosphorylation of the translation initiation factor eIF2alpha increases BACE1 levels and promotes amyloidogenesis. - AlzForum Alzheimer Research Paper of the Week


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Home: Papers of the Week

Annotation

	O'Connor T, Sadleir KR, Maus E, Velliquette RA, Zhao J, Cole SL, Eimer WA, Hitt B, Bembinster LA, Lammich S, Lichtenthaler SF, Hébert SS, De Strooper B, Haass C, Bennett DA, Vassar R. Phosphorylation of the translation initiation factor eIF2alpha increases BACE1 levels and promotes amyloidogenesis. Neuron. 2008 Dec 26;60(6):988-1009. PubMed Abstract

	Comments on Paper and Primary News

	Comment by: George Perry (Disclosure)
	Submitted 7 January 2009 \| Permalink	Posted 8 January 2009
	I recommend this paper

	Comments on Related Papers

	Related Paper: The E693Delta mutation in amyloid precursor protein increases intracellular accumulation of amyloid beta oligomers and causes endoplasmic reticulum stress-induced apoptosis in cultured cells. Comment by: George Perry (Disclosure)
	Submitted 10 February 2009 \| Permalink	Posted 11 February 2009
	I recommend this paper

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REAGENTS/MATERIAL:
Immunoprecipitation: rabbit anti-BACE1 (PA1-757; Affinity Bioreagents)
Immunohistochemistry: monoclonal mouse anti-Aβ total (4G8) (MAB1561; Chemicon)
ELISA: standards made from purified recombinant human Aβ40 peptide were added in duplicate to a pre-coated human Aβ40-specific sandwich ELISA plate (BioSource, Camarillo, CA)
Western Blotting: monoclonal mouse anti-BACE-1 (3D5) (Zhao, 2007); monoclonal mouse anti-fIAPP (22C1) (MAB348, Chamicon); monoclonal mouse anti-β-Actin (AC-15) (A1978, Sigma); monoclonal rabbit anti-eIF2a-Phos Ser51 (119A11) (3597, Cell Signaling); rabbit anti-eIF2a (poly) (9722, Cell Signaling); rabbit anti-c-Jun-Phos Ser63 (poly) (9261, Cell Signaling); rabbit anti-c-Jun (9162, Cell Signaling); monoclonal mouse anti-JNK-Phos Thr183/185 (G9) (9255, Cell Signaling); rabbit anti-elF4E-Phos Ser209 (9741, Cell Signaling); rabbit anti-JNK (9252, Cell Signaling); rabbit anti-Caspase 3 (9662, Cell Signaling); goat anti-Neprilysin (AF1126, R&D Systems); rabbit anti-IDE (ab32216, Abcam) and rabbit anti-PS1-NT (gift from Dr. Gopal Thinakaran, University of Chicago)