Winkler DT, Bondolfi L, Herzig MC, Jann L, Calhoun ME, Wiederhold KH, Tolnay M, Staufenbiel M, Jucker M. Spontaneous hemorrhagic stroke in a mouse model of cerebral amyloid angiopathy. J Neurosci. 2001 Mar 1;21(5):1619-27. PubMed Abstract

REAGENTS/MATERIAL:
Generation of B6,D2-TgN (Thy1-APPSwe)23 Trasgenic mice as described in Sturchler-Pierrat et al). APP23 mice overexpress APP751 with the Swedish double mutation under the control of a neuron-specific Thy-1 promoter element, backcrossed with C57BL/6J mice. Cresyl violet, hematoxylin and eosin (H&E), and Congo red staining were done. Avidin-biotin-peroxidase complex method (Vector Laboratories) with diaminobenzidine as chormogen. Polyclonal antibody AS42/14 specifically to Ab42 Polyclonal antibody FCA3340 and FCA3542 specifically to Ab40 and Ab42, respectively, gift from F. Checlair. Mouse monoclonal antody to a-smooth muscle actin, clone 1A4; (Sigma) Mouse monoclonal antibody to b-dystroglycan (Novocastra) Polyclonal antibody to glial fibrillary acidic protein (GFAP) (Dako) Polyclonal antibody to cystatin C (Accurate Chemicals) Polyclonal antibody to mouse serum amyloid P component (SAP) (Calbiochem) Polyclonal antibody to AB (NT12) Secondary antibodies, Alexa 568 goat anti-rabbit IgG, Alexa 488 goat anti-mouse IgG (Molecular Probes)

FUTURE DIRECTION:
Further evaluation of CAA in AD dementia needed. Vaccination in APP23 mice might be a good study tool on CAA since PDAPP transgenic mice do not develop significant CAA.