Vetrivel KS, Meckler X, Chen Y, Nguyen PD, Seidah NG, Vassar R, Wong PC, Fukata M, Kounnas MZ, Thinakaran G. Alzheimer disease Abeta production in the absence of S-palmitoylation-dependent targeting of BACE1 to lipid rafts. J Biol Chem. 2009 Feb 6;284(6):3793-803. PubMed Abstract

REAGENTS/MATERIAL:
Antibodies used in this study:
BACE1 was detected using rabbit anti-BACE1 [residues 46-163] and 7523 [residues 46-60] (provided by Dr. Christian Haass, Ludwig-Maximilians-University, Munich), or monoclonal anti-BACE1-Cat1 [residues 46-460] and anti-FLAG M2 (Sigma).
Rabbit polyclonal antiserum CTM1 was raised against a synthetic peptide corresponding to the C-terminal 15 amino acids of APP followed by the c-Myc epitope (MEQKLISEEDLN). Rabbit polyclonal anti-PS1_NT (residues 1-65) has been described.
Both mAb anti-Aβ 26D6 and anti-Aβ (B436) (epitope within residues 1-12 of Aβ) react with the amino-terminal region of Aβ and also recognize sAPPα; mAb B113 is selective for Aβ40.
The following antibodies were purchased from commercial sources:
monoclonal mouse anti-GM130 (35) (BD Biosciences); monoclonal mouse anti-syntaxin 6 (30) (BD Biosciences); monoclonal mouse anti-flotillin-2 (29) (BD Biosciences); monoclonal mouse anti-γ-adaptin (88) (BD Biosciences); monoclonal mouse anti-EEA1 (14) (BD Biosciences); monoclonal mouse anti-HA (16B12) (Covance Research Products); monoclonal mouse anti- Aβ (4G8) (Covance) and monoclonal mouse anti-APP, secretory (OM84) (Chemicon Millipore).