Human embryonic stem cell-derived motor neurons are sensitive to the toxic effect of glial cells carrying an ALS-causing mutation. - AlzForum Alzheimer Research Paper of the Week


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Annotation

	Di Giorgio FP, Boulting GL, Bobrowicz S, Eggan KC. Human embryonic stem cell-derived motor neurons are sensitive to the toxic effect of glial cells carrying an ALS-causing mutation. Cell Stem Cell. 2008 Dec 4;3(6):637-48. PubMed Abstract

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REAGENTS/MATERIAL:
Antibodies used in this study were:
monoclonal mouse anti-β-Tubulin III (TUJ1) (Covance); rabbit anti-β-Tubulin III (Sigma Aldrich); monoclonal mouse anti-Pax6 (PAX6) (DSHB, U. Iowa); monoclonal mouse anti-Nkx6.1 (F65A2) (DSHB, U. Iowa); monoclonal mouse anti-Nkx2.2 (74.5A5) (DSHB, U. Iowa); monoclonal mouse anti-Isl-1 (DSHB, U. Iowa); monoclonal mouse anti-Hb9 (81-5C10) (DSHB, U. Iowa, Tom M. Jessell and Susan Morton); goat anti-Chx10 (Santa Cruz); anti-Lhx2 (Santa Cruz) and anti-ChAT (Chemicon)