Non-cell-autonomous effect of human SOD1 G37R astrocytes on motor neurons derived from human embryonic stem cells. - AlzForum Alzheimer Research Paper of the Week


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Home: Papers of the Week

Annotation

	Marchetto MC, Muotri AR, Mu Y, Smith AM, Cezar GG, Gage FH. Non-cell-autonomous effect of human SOD1 G37R astrocytes on motor neurons derived from human embryonic stem cells. Cell Stem Cell. 2008 Dec 4;3(6):649-57. PubMed Abstract

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REAGENTS/MATERIAL:
The primary antibodies used were:
For immunofluorescence:
monoclonal mouse anti-Pax6 (PAX6) (DSHB, U. Iowa, Iowa City, IA); monoclonal mouse anti-Islet 1 (DSHB, U. Iowa); monoclonal mouse anti-Hb9 (81-5C10) (DSHB U. Iowa); monoclonal mouse anti-human Nestin (rat-401) (Chemicon Millipore); rabbit anti-Olig2 (Chemicon); anti-ChAT (Chemicon); monoclonal mouse anti-A2B5 (A2B5-105) (Chemicon); monoclonal mouse anti-βIII-tubulin (TuJ1) (Covance Research Products); monoclonal mouse anti-HoxC8 (C952-12E) (Covance Research Products); anti-GFP (Molecular Probes-Invitrogen, CA); anti-GFAP (DAKO, Carpinteria, CA); and monoclonal mouse anti-GAD65 (GAD-6) (Sigma-Aldrich, St Louis, MO).
Assessment of virus titering using monoclonal mouse anti-human SOD1 (SD-G6) (Sigma-Aldrich)
For Western Blotting:
monoclonal mouse anti-actin (AC-15) (Ambion, Austin, TX); rabbit anti-iNOS (Abcam, Cambrige, MA); monoclonal mouse anti-CHGA (23A1) (Abcam); rabbit anti-CC (Abcam) and rabbit anti-NOX2 (Abcam)