Julien C, Tremblay C, Phivilay A, Berthiaume L, Emond V, Julien P, Calon F. High-fat diet aggravates amyloid-beta and tau pathologies in the 3xTg-AD mouse model. Neurobiol Aging. 2010 Sep;31(9):1516-31. PubMed Abstract

REAGENTS/MATERIAL:
Antibodies used include:
anti-actin (Chemicon International, Temecula, CA); rabbit anti-Amyloid β/A4 Protein Precursor770 (C31) (Bachem, Torrance, CA) for C-terminal fragment of APP (CTF-α and CTF-β); monoclonal mouse anti-Aβ (6E10) (Covance) for APP measures; rabbit anti-amyloid oligomers (A11) (Millipore, Billerica, MA) to detect soluble amyloid oligomers; anti-Apolipoprotein E (Novus Biologicals, Littleton, CO); rabbit anti-Cofilin (Cell Signaling); monoclonal mouse anti-Drebrin (MX823) (Progen, Heidelberg, Germany); and monoclonal mouse anti-Drebrin (M2F6) (MBL, Woburn, MA); anti-GFAP (Sigma–Aldrich); monoclonal mouse anti-LR11/SorLA/gp250 (48/LR11) (BD Biosciences, San Jose, CA); rabbit anti-PAK1/2/3 (Cell Signaling Technology); anti-PSD-95 (clone K28/43, discontinued) (Upstate Biotechnology, Lake Placid, NY); monoclonal mouse anti-SNAP-25 (Covance); monoclonal mouse anti-Synaptophysin (SVP-38) (Chemicon International); rabbit anti-Syntaxin 3 (Novus Biologicals, Littleton, CO); monoclonal mouse anti-tau (tau-13) (Covance, Berkeley, CA); monoclonal mouse anti-phosphorylated tau (PHF1) (Dr Peter Davies, Albert Einstein College of Medicine); monoclonal mouse anti-phosphorylated tau (CP13) (Dr Peter Davies, Albert Einstein College of Medicine) and monoclonal mouse anti-tau (AT270) (1:500; Pierce).
ELISA:
Insoluble Aβ40 and Aβ42 were measured by the βAmyloid [1–40] and βAmyloid [1–42] ELISA kits (Biosource, Camarillo, CA). Soluble Aβ40 and Aβ42 were measured using anti-Human β Amyloid (1–42) ELISA kit (WAKO, Osaka, Japan).