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REAGENTS/MATERIAL:
Primary antibodies used were:
rabbit anti-JAK2, phos Tyr1007/1008 (Cell Signaling, Beverly, MA, USA);
rabbit anti-STAT3, phos Tyr705 (Cell Signaling);
rabbit ERK1/2, phos Thr202/Tyr204 (Cell Signaling);
monoclonal rabbit anti-total-JAK2 (24B11, discontinued) (Cell Signaling);
monoclonal rabbit anti-total-STAT3 (79D7) (Cell Signaling);
goat anti-ChAT (Chemicon, Temecula, CA, USA);
monoclonal mouse anti-Aβ1–16 (6E10) (Biosource –discontinued);
rabbit anti-total-ERK1 (Santa Cruz Biotechnology, Santa Cruz, CA, USA);
rabbit anti-SOCS3 (IBL, Gunma, Japan);
rabbit anti-SHP2 (Abcam, Cambridge, UK);
rabbit anti-PIAS3;
rabbit anti-actin (Sigma-Aldrich, St. Louis, MO, USA);
rabbit anti-MAP2 (Sigma-Aldrich);
rabbit anti-mAChR, M1 (Santa-Cruz);
rabbit anti-mAChR, M1 (Abcam);
rabbit anti-p75 NTR (Santa-Cruz);
monoclonal mouse anti-Aβ40 (BAN50/BA27) (Wako Chemicals) and
monoclonal mouse anti-Aβ42 (BAN50/BC05) (Wako Chemicals)
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