Anderson JM, Hampton DW, Patani R, Pryce G, Crowther RA, Reynolds R, Franklin RJ, Giovannoni G, Compston DA, Baker D, Spillantini MG, Chandran S. Abnormally phosphorylated tau is associated with neuronal and axonal loss in experimental autoimmune encephalomyelitis and multiple sclerosis. Brain. 2008 Jul;131(Pt 7):1736-48. PubMed Abstract

REAGENTS/MATERIAL:
Primary antibodies used were:
mouse monoclonal anti-glial fibrillary acid protein-Cy3 conjugate (G-A-5) (Sigma, Poole, UK); biotinylated mouse monoclonal anti-Neuronal Nuclei (NeuN) (A60) (Chemicon, Chandlers Ford, UK); rat monoclonal anti-F4/80 (CI:A3-1) (AbD Serotec, Kidlington, UK); rat polyclonal antibody specific for myelin basic protein (MBP) (AbD Serotec, UK), mouse monoclonal anti-tau phospho Ser202/Thr205 (AT8) (Autogen Bioclear); rabbit polyclonal anti-neurofilament 200kD (Sigma Aldrich); mouse monoclonal phospho-independent anti-tau (Tau5) (Calbiochem, Nottingham, UK); mouse monoclonal anti-tau phospho Thr231/Ser235 (AT180) (Autogen Bioclear); mouse monoclonal anti-tau phospho Thr181 (AT270) (Autogen Bioclear); mouse monoclonal anti-tau phospho Ser212/Thr214 (AT100) (Autogen Bioclear); mouse monoclonal anti-tau phospho Ser396/Ser404 (PHF-1) (gift from Dr P Davies, Albert Einstein College of Medicine, Bronx, NY); antiserum MT1 recognizes the three adult mouse tau isoform but fails to recognize human tau isoforms (kind gift from Dr M. Goedert, MRC Laboratory of Molecular Biology, Cambridge, England); antiserum BR134 recognizes the C terminus of both human and mouse tau, was performed as described previously (Goedert et al., 1992); mouse monoclonal anti-human B lymphocyte (LN3) (MP Biomedicals, Cambridge, UK); mouse mAb anti-human GFAP (1: 500; Covance, Harrogate, UK); mouse monoclonal anti-APP-A4 (22C11) (Chemicon) and mouse monoclonal anti-non-phopsphorylated neurofilament (SMI-32) (Covance)