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Levels of Aβ1–40
and Aβ1–42 were measured using commercially available ELISA kits
(Biosource/Invitrogen) according to the manufacturer’s instructions.
GR and CRF-R1 immunohistochemical staining was performed using published methods
(Chen et al., 2000;
Klimaviciute et al., 2006). Antibodies used were
mouse monoclonal anti-GR (BuGR2) (Affinity BioReagents) and
goat anti-CRF-R1 (Santa Cruz Biotech).
For immunohistochemical staining of Aβ plaques,
rabbit anti-Aβ pan (Biosource/Invitrogen)
raised against Aβ synthetic peptide, in the region of amino acids 15–30.