Critical role of CDK5 and Polo-like kinase 2 in homeostatic synaptic plasticity during elevated activity. - AlzForum Alzheimer Research Paper of the Week


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Home: Papers of the Week

Annotation

	Seeburg DP, Feliu-Mojer M, Gaiottino J, Pak DT, Sheng M. Critical role of CDK5 and Polo-like kinase 2 in homeostatic synaptic plasticity during elevated activity. Neuron. 2008 May 22;58(4):571-83. PubMed Abstract

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REAGENTS/MATERIAL:
Phospho-specific a-pS1328 SPAR antibodies were generated by immunizing rabbits (Covance Research Products) with a SPAR peptide corresponding to residues 1323–1335, in which S1328 was phosphorylated. Phospho-antibodies were purified first by negative selection over a column of covalently attached unphosphorylated peptide, followed by binding to a column of phosphorylated peptide. Polyclonal GKBD-SPAR antibodies were generated by immunizing rabbits with purified MBP-GKBD fusion protein. The polyclonal C-SPAR antibody has been described in Pak et al., 2001. Mouse monoclonal PSD-95 antibody K28/43 was a gift from J. Trimmer (UC Davis); rabbit polyclonal p25/p35 antibody was a gift from Li-Huei Tsai (MIT).
The following commercial antibodies were used: mouse monoclonal anti-myc, agarose conjugate (9E10) (Santa Cruz); rabbit anti-GST (Z-5) (Santa Cruz Biotechnology); mouse monoclonal anti-α-tubulin (B-5-1-2) (Sigma Aldrich); rabbit anti-GFP (MBL International)