Damiani D, Alexander JJ, O'Rourke JR, McManus M, Jadhav AP, Cepko CL, Hauswirth WW, Harfe BD, Strettoi E. Dicer inactivation leads to progressive functional and structural degeneration of the mouse retina. J Neurosci. 2008 May 7;28(19):4878-87. PubMed Abstract

REAGENTS/MATERIAL:
Primary antibodies and dilutions were as follows:
mouse monoclonal anti-rhodopsin (1D4) (Sigma Aldrich); rabbit anti-recoverin (Chemicon); mouse monoclonal anti-protein kinase C-α (MC5) (Sigma Aldrich); rabbit anti-protein kinase C-α (sc-208) (Santa Cruz Biotechnology); mouse monoclonal anti-calbindin D-28k (CB955) (Sigma Aldrich); rabbit anti-calbindin D-28k (sc-280) (Swant); mouse monoclonal anti-Goα (2A) (Chemicon); mouse monoclonal anti--neurofilament 200 kDa (N52) (Sigma Aldrich); rabbit anti-mGluR6 (1:2000; from Dr. S. Nakanishi, Osaka University, Osaka, Japan); mouse anti-postsynaptic density protein 95 (PSD95) (AbCam, UK): mouse monoclonal anti-glutamine synthase (GS-6) (Chemicon); rabbit anti-GFAP (Sigma Aldrich); goat anti-choline acetyl transferase (Chemicon); mouse monoclonal anti-Cre recombinase (2D8) (Chemicon); rabbit anti-GFP–Alexa Fluor 488 (Invitrogen); rabbit anti-phosphohistone H3 (Chemicon Millipore); rabbit anti-atypical PKC (C20) (Santa Cruz Biotechnology) rabbit anti-zonula occludens-1 (Zymed Invitrogen); rabbit anti-laminin (Sigma Aldrich); guinea pig anti-vesicular glutamate transporter (Chemicon) and rabbit anti-vesicular GABA transporter (Synaptic Systems); Rabbit anti-S-cone opsin (1:1000; Chemicon) and Alexa Fluor 488-PNA lectins (1:400; Invitrogen) were used to label cone photoreceptors.