Synaptic NMDA receptor activity boosts intrinsic antioxidant defenses. - AlzForum Alzheimer Research Paper of the Week


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Home: Papers of the Week

Annotation

	Papadia S, Soriano FX, Léveillé F, Martel MA, Dakin KA, Hansen HH, Kaindl A, Sifringer M, Fowler J, Stefovska V, McKenzie G, Craigon M, Corriveau R, Ghazal P, Horsburgh K, Yankner BA, Wyllie DJ, Ikonomidou C, Hardingham GE. Synaptic NMDA receptor activity boosts intrinsic antioxidant defenses. Nat Neurosci. 2008 Apr;11(4):476-87. PubMed Abstract

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REAGENTS/MATERIAL:
Antibodies used in this study, for Western blotting, are:
rabbit anti-phospho-FKHR (Thr24) (Cell Signaling); rabbit anti-FKHR (Cell Signaling); mouse monoclonal anti-beta-tubulin, isotype III (SDL.3D10) (Sigma-Aldrich); rabbit anti-Thioredoxin (Santa Cruz); mouse monoclonal anti-Txnip/VDUP-1 (JY1) (MBL); mouse monoclonal anti-2 Cys Peroxiredoxin (6E5) (Abcam); goat anti-Sulfiredoxin (P-16) (Santa Cruz); rabbit anti-Sestrin 2 (Cell Signaling); rabbit anti-Prx-SO2/3H (Abcam); rabbit anti-Thioredoxin Reductase (Abcam); mouse monoclonal anti-phospho-Ser 473-Akt (587F11) (Cell Signaling); rabbit anti-Akt (Cell Signaling); rabbit anti-Txnip/VDUP-1 (Zymed Invitrogen);
For Immunofluorescence: monoclonal mouse anti-myc (9E10) (Santa Cruz); goat anti-Sulfiredoxin (S-17) (Santa Cruz); mouse monoclonal anti-NeuN (A60) (Chemicon Millipore) and mouse monoclonal anti-GFAP (G-A-5) (Sigma Aldrich);