Active gamma-secretase complexes contain only one of each component. - AlzForum Alzheimer Research Paper of the Week


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Annotation

	Sato T, Diehl TS, Narayanan S, Funamoto S, Ihara Y, De Strooper B, Steiner H, Haass C, Wolfe MS. Active gamma-secretase complexes contain only one of each component. J Biol Chem. 2007 Nov 23;282(47):33985-93. PubMed Abstract

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REAGENTS/MATERIAL:
Antibodies used in this study are:
mouse monoclonal anti-Amyloidβ (6E10) (CovanceResearch Products); mouse monoclonal anti-FLAG (M2) (Sigma Aldrich); rabbit monoclonal anti-penta-HIS (Quiagen); rabbit anti-PS1 (B17) (B. De Strooper); mouse monoclonal anti-myc (Invitrogen); mouse monoclonal anti-HA (HA-7) (Sigma Aldrich); Aβ40 and Aβ42 were detected by sensitive and specific ELISAs; anti-IgG (protein G Plus/protein A-agarose)(EMD Calbiochem); rabbitanti-Nicastrin (Sigma Aldrich); rabbit anti-PS1-CTF (4627) (D. Selkoe); mouse monoclonal anti-PS1-NTF (AB14) (S. Gandy); rabit anti-myc (Sigma Aldrich) rat monoclonal anti-HA (3F10) (Roche Applied Science); mouse monoclonal anti-HIS-agarose (HIS-1) (Sigma Aldrich); rabbit anti-ECS (Bethyl); rabbit anti-Aph-1 (Affinity BioReagents); rabbit anti-Aph-1aL and Aph-1aS (H. Steiner, C. Hass); rabbit anti-Biotin (Bethyl); rabbit anti-APP (C9) (D. Selkoe); rabbit anti-TMP21 (I. Schulz); rat monoclonal anti-PS1 (hPS1-NT) (Chemicon Millipore); rabbit anti-PEN2 (Abcam); rabbit anti-GST (Zymed Invitrogen)