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Nielsen MS, Gustafsen C, Madsen P, Nyengaard JR, Hermey G, Bakke O, Mari M, Schu P, Pohlmann R, Dennes A, Petersen CM.
Sorting by the cytoplasmic domain of the amyloid precursor protein binding receptor SorLA. Mol Cell Biol.
2007 Oct;27(19):6842-51.
PubMed Abstract, View on AlzSWAN
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REAGENTS/MATERIAL:
Antibodies used in this study are:
primary rabbit antibodies raised against SorLA-Vps10p-D and a monoclonal anti-Sortilin-Vps10p-D antibody,
specific antibodies against PACS-1 (a gift from Gary Thomas), goat anti-Vps35 (Abcam), chicken anti-AP-2 μ-chain (Abcam), rabbit anti-AP-1 μ-chain, rabbit anti-SNX1 (Abcam), rabbit anti-SNX1 (Bethyl Labs), rabbit anti-human TGN46 (Serotec, Norway),
rat monoclonal anti-mouse Lamp1/2 (GL2A7) (Hybridoma Bank, Iowa), monoclonal antibody (2C2) recognizing a luminal epitope of MPR300, mouse monoclonal anti-AP-1 γ (88) (BD Transduction),
mouse monoclonal anti-AP-2 α (8) (BD Transduction), mouse monoclonal anti-vti 1a (45) (BD Transduction), mouse monoclonal anti-vti 1b (7) (BD Transduction), anti-LRP (LDL-related protein (LRP)/LR1)
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Retromer sorting: a pathogenic pathway in late-onset Alzheimer disease.
