Englund H, Sehlin D, Johansson AS, Nilsson LN, Gellerfors P, Paulie S, Lannfelt L, Pettersson FE. Sensitive ELISA detection of amyloid-beta protofibrils in biological samples. J Neurochem. 2007 Oct;103(1):334-45. PubMed Abstract

REAGENTS/MATERIAL:
Antibodies used in this study, for Sandwich ELISA and Western Blotting:
Two clones were generated to amyloid-beta protofibrils, producing mouse monoclonal mAb158 (IgG2a) and mAb1C3 (IgG1).
mouse monoclonal Anti-α-synuclein (211) (Santa Cruz), Anti-islet amyloid polypeptide (IAPP) polyclonal antibody pAb A110 (gift from P. Westermark, Uppsala University) and the anti-medin polyclonal antibody pAb 179 (gift from A. Larsson, Uppsala University) were used as positive controls in experiments on amyloidogenic proteins other than Aβ; mouse monoclonal Anti-Amyloidβ, anti-APP (6E10) (Signet), was used as control anti-Aβ antibody;