Double mutant mice were generated by crossing hemizygote Tg2576 3 hemizygote JNPL3. Mice were maintained on a SW/DBA2/C57B6 background.
The following tau antibodies were used:
Ab39 (Yen, 1:10);
Alz50 (Davies, 1:10);
PHF1 (Davies, 1:100);
TG3 (Davies, 1:10);
CP3 (Davies, 1:100);
CP13 (Davies, 1:100);
PG5 (Davies, 1:100);
E-1 (Yen, 1:1000);
RT97 (Anderton, 1:100);
AT180 (Innogenetics, 1:100); polyclonal antibody to ubiquitin, UH19 (Ksiezak-Reding, 1:500);
a polyclonal antibody to amyloid-beta-crystallin (Novacastra, 1:250); an antibody to mitotic phosphoepitopes, MPM-2 (Accurate 1:200);monoclonal glial fibrillary acidic protein (GFAP) (Biogenex, 1:100); monoclonal APP (22C11) (Roche, 1:20);
Conformational tau epitopes: Alz50, MC-1, and Ab39.
Phosphorylated tau epitopes: RT97 (phospho-46), CP13 (phospho-202/205), CP3 (phospho-214), AT180 (phospho-231/235), TG3 (phospho-231/235), PHF-1 (phospho-396/404), and PG5 (phospho-409).
Other antibodies: polyclonal E1 (human-specific tau exon 1 aa 19-33), polyclonal WKS45 antibody that recognizes mouse and human tau (aa 258-266), PHF-1 (phopho-396/404)for hyperphosphorylated tau, beta-tubulin (Sigma)and MPM-2.
ELISA sandwich using BAN50/BA27 and BAN50/BC05 for amyloid-beta 40 and 42.