Antibodies and dilutions used in this study include 6E10 (1:1000; Signet, Dedham, MA) for APP in in vivo studies, CTF20 (1:5000; Calbiochem, San Diego, CA) for C99 and C83, 22C11 (1:1000; Chemicon, Temecula, CA) and HT7 (1:3000; Innogenetics, Gent, Belgium) for APP in in vitro studies, AT8 (1:1000; Pierce), AT180 (1:1000; Pierce), anti-caspase cleaved APP (cAPP) (1:3000; Chemicon), anti-BACE (1:1000; Calbiochem), anti-glucocorticoid receptor (1:300; Affinity BioReagents, Golden, CO), and b-actin (1:10,000; Sigma, St. Louis, MO).
3XTg-AD mice were used in this study.
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