The regulation of tau phosphorylation by PCTAIRE 3: implications for the pathogenesis of Alzheimer's disease. - AlzForum Alzheimer Research Paper of the Week


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Annotation

	Herskovits AZ, Davies P. The regulation of tau phosphorylation by PCTAIRE 3: implications for the pathogenesis of Alzheimer's disease. Neurobiol Dis. 2006 Aug;23(2):398-408. PubMed Abstract

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REAGENTS/MATERIAL:

Samples were probed with antibodies against pKAβ (C-20; Santa Cruz), GSK3β (Cell Signaling), anti-cdk1/cdc2 (Upstate), cdk5 (J3; Santa Cruz), PCTAIRE 1 (gift of Dr. Julian Gannon and Dr. Tim Hunt) and β-actin (Sigma) according to the dilutions recommended by the providers. Antibodies raised in our laboratory including PCTAIRE 3 antibodies (1D3, 2C7 and 52G6) and tau antibodies (CP9, CP13, PHF1, CP3, PG5 and CP27) were used at a concentration of 1:20.