Four different transgenic mouse models of AD were used in the current studies, R1.40, Tg2576, and Tg2576/PSEN1 and APP23.
Immunocytochemistry used the following antibodies: proliferating cell nuclear antigen (PCNA) mouse monoclonal antibody recognizing the PCNA p36 protein (Dako) was diluted 1:250. The rabbit polyclonal cyclin A antibody (ab 7956; working dilution, 1:200; Abcam) raised against the C-terminal domain of cyclin A2. The mouse monoclonal NeuN antibody (dilution, 1:500; Chemicon) was used as a neuronal marker. The rat monoclonal CD45 antibody (catalog #MCA1388; working dilution, 1:500; Serotec) was used as a microglial maker.